Abstract
Full-thickness skin grafts are used in reconstructive surgeries. Objectives Experimental study of the possibility of long-term preservation of viable skin grafts in severely impaired trophics at remote interaction with entities having stratiform periodic structure. Material and methods Full-thickness skin was excised from the rabbits' backside and dissected into explants sized 1.0 × 1.0 cm. The samples (n = 81) were divided into three groups and thermostated for 2 days at 37 °C at a various distance from the metal (aluminum) presented as a 20-layer package of smooth foil forming a stratiform periodic structure (SPS) (series I), chaotic layers of squeezed foil (series II) and a single-piece sheet (series III). Histological analysis was performed for the three series to evaluate the explants' viability after the thermostating. Results The highest survival estimates were seen in experimental explants of series I that interacted with the SPS of stratified foil layers. The wave nature of such remote interaction was suggested with delayed dystrophic and necrotic processes developing in the skin samples. Experimental samples of series III appeared to be less viable. The explant vitality in series II was sharply reduced due to rapidly spreading necrosis. Conclusion Skin explants were shown to retain viability for a longer time when interacting remotely with stratiform periodic structures in the absent trophics. These promising results can be practical for the development of wound dressings to improve survival of full-thickness skin transplantation in reconstruction of deep skin defects.
Highlights
Full-thickness skin grafts are used in reconstructive surgery to cover extensive skin lesions caused by deep burns
The muscle layer of rabbits located between the dermis and subcutaneous tissue was represented by striated tissue, muscle fibers with many elongated nuclei and connective tissue layers with nuclei well stained and identified (Fig. 2)
The sections showed total necrosis of the epithelium and the dermis with only the contours remained from the hair follicles
Summary
Full-thickness skin grafts are used in reconstructive surgery to cover extensive skin lesions caused by deep burns. With respect to skin regeneration, there is a limited number of papers to support the stimulating effect of SPS on cellular activity despite the use of composite scaffold matrices in the clinic [9], and more studies are needed to explore this topic. This necessitates the search for optimal wound coverings with the current medical needs for a high-quality wound treatment system to serve the basis for the development of new technologies for regenerative surgery
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