Abstract
Ð’ N€Ð°Ð±Ð¾N‚е Ð?N€Ð¾Ð²ÐµÐ´ÐµÐ½ NN€Ð°Ð²Ð½Ð¸N‚елNŒÐ½N‹Ð¹ анализ моN€N„ологиN‡ÐµNÐoой NN‚N€NƒÐoN‚NƒN€N‹, NндоÐoN€Ð¸Ð½Ð½Ð¾Ð¹ N„NƒÐ½ÐoN†Ð¸Ð¸ и N„ол-лиÐoNƒÐ»Ð¾Ð³ÐµÐ½Ð·Ð° N‚N€Ð°Ð½NÐ?ланN‚аN‚ов Ð?оловозN€ÐµÐ»Ð¾Ð¹ и неонаN‚алNŒÐ½Ð¾Ð¹ оваN€Ð¸Ð°Ð»NŒÐ½Ð¾Ð¹ N‚Ðoани ÐoN€N‹N Ð?оNле гиÐ?оN‚еN€Ð¼Ð¸N‡ÐµNÐoого N…N€Ð°Ð½ÐµÐ½Ð¸N (ГХ) в NN€ÐµÐ´Ð°N… N€Ð°Ð·Ð»Ð¸N‡Ð½Ð¾Ð³Ð¾ NоNN‚ава. ДлN вN‹Ð?олнениN N€Ð°Ð±Ð¾N‚N‹ бN‹Ð»Ð¸ иNÐ?олNŒÐ·Ð¾Ð²Ð°Ð½N‹ NледNƒNŽN‰Ð¸Ðµ меN‚одN‹: геN‚еN€Ð¾N‚оÐ?иN‡ÐµNÐoаN N‚N€Ð°Ð½NÐ?ланN‚аN†Ð¸N (иNNледование NN‚N€NƒÐoN‚NƒN€N‹ и N„NƒÐ½ÐoN†Ð¸Ð¸ оваN€Ð¸Ð°Ð»NŒÐ½Ð¾Ð¹ N‚Ðoани Ð?оNле ГХ); иммNƒÐ½Ð¾-N„еN€Ð¼ÐµÐ½N‚нN‹Ð¹ анализ (оÐ?N€ÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ ÐoонN†ÐµÐ½N‚N€Ð°N†Ð¸Ð¸ Ð?оловN‹N… гоN€Ð¼Ð¾Ð½Ð¾Ð² в Ð?лазме); моN€N„ологиN‡ÐµNÐoий (оN†ÐµÐ½Ðoа N†ÐµÐ»Ð¾NN‚-ноNN‚и NN‚N€NƒÐoN‚NƒN€N‹ N‚N€Ð°Ð½NÐ?ланN‚аN‚ов N‚Ðoани Ð?оNле ГХ). ÐÐoNÐ?еN€Ð¸Ð¼ÐµÐ½N‚алNŒÐ½Ð¾ доÐoазано, N‡N‚о оÐ?N€ÐµÐ´ÐµÐ»NNŽN‰Ð¸Ð¼ N„аÐoN‚оN€Ð¾Ð¼ N„оллиÐoNƒÐ»NN€Ð½Ð¾Ð³Ð¾ N€Ð°Ð·Ð²Ð¸N‚иN и NндоÐoN€Ð¸Ð½Ð½Ð¾Ð¹ N„NƒÐ½ÐoN†Ð¸Ð¸ N‚N€Ð°Ð½NÐ?ланN‚аN‚ов оваN€Ð¸Ð°Ð»NŒÐ½Ð¾Ð¹ N‚Ðoани N€Ð°Ð·Ð»Ð¸N‡Ð½N‹N… NN‚адий гиNN‚огенеза Ð?оNле ГХ NвлNеN‚NN NоNN‚ав NN€ÐµÐ´N‹ инÐoNƒÐ±Ð°N†Ð¸Ð¸. УNN‚ановлено, N‡N‚о Ð?оNле Ð?N€Ð¸Ð¼ÐµÐ½ÐµÐ½Ð¸N N„оNN„аN‚но-Nолевого бNƒN„еN€Ð° длN ГХ знаN‡Ð¸Ð¼Ð¾ NƒÐ¼ÐµÐ½NŒNˆÐ°Ð»NN N„оллиÐoNƒÐ»NN€Ð½N‹Ð¹ Ð?NƒÐ» и NнижалаNNŒ NN‚еN€Ð¾Ð¸Ð´Ð¾Ð³ÐµÐ½Ð½Ð°N N„NƒÐ½ÐoN†Ð¸N N‚N€Ð°Ð½NÐ?ланN‚аN‚ов ÐoаÐo Ð?оловозN€ÐµÐ»Ð¾Ð¹, N‚аÐo и неонаN‚алNŒÐ½Ð¾Ð¹ оваN€Ð¸Ð°Ð»NŒÐ½Ð¾Ð¹ N‚Ðoани. ПоÐoазано, N‡N‚о в N€ÐµÐ·NƒÐ»NŒN‚аN‚е гиÐ?оN‚еN€Ð¼Ð¸N‡ÐµNÐoого N…N€Ð°Ð½ÐµÐ½Ð¸N в NN€ÐµÐ´Ðµ, NодеN€Ð¶Ð°N‰ÐµÐ¹ манниN‚ол, ÐoолиN‡ÐµNN‚во N„оллиÐoNƒÐ»Ð¾Ð² (N€Ð°Ð·Ð½Ð¾Ð¹ NN‚еÐ?ени зN€ÐµÐ»Ð¾NN‚и и желN‚N‹Ðµ N‚ела) и NндоÐoN€Ð¸Ð½Ð½Ð°N N„NƒÐ½ÐoN†Ð¸N Ð?оNле N‚N€Ð°Ð½NÐ?ланN‚аN†Ð¸Ð¸ оваN€Ð¸Ð°Ð»NŒÐ½Ð¾Ð¹ N‚Ðoани бN‹Ð»Ð¸ NN€Ð°Ð²Ð½Ð¸Ð¼N‹ N N‚аÐoовN‹Ð¼Ð¸ Ð?оNле N‚N€Ð°Ð½NÐ?ланN‚аN†Ð¸Ð¸ NвежевN‹Ð´ÐµÐ»ÐµÐ½Ð½Ð¾Ð¹ N‚Ðoани.Probl Cryobiol Cryomed 2019; 29(1): XXX–XXXÂ
Highlights
There were comparatively analyzed the morphology, endocrine function and folliculogenesis of mature and neonatal rat ovarian tissue grafts after hypothermic storage (HS) in the media with different composition
When analyzing the morphological structure of the grafts of subgroups 1A and 3A there were revealed the follicular cysts, the inner wall of which was lined with several layers of elongated fibroblast-like cells with content [21]
The analysis of morphological structure of mature ovarian tissue grafts in animals of all the studied groups demonstrated the presence of follicles of different maturity degrees and corpus luteum
Summary
There were comparatively analyzed the morphology, endocrine function and folliculogenesis of mature and neonatal rat ovarian tissue grafts after hypothermic storage (HS) in the media with different composition. It was shown that when using the mannitol-containing solution for HS, the number of follicles of different maturity degree and corpus luteum and the endocrine function after ovarian tissue transplantation were comparable with those after fresh tissue transplantation. В связи с вышеизложенным целью работы было проведение сравнительного анализа морфологической структуры и эндокринной функции трансплантатов половозрелой и неонатальной овариальной ткани после гипотермического хранения в фосфатно-солевом буфере и маннитол-содержащем растворе
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