Endocrine Function of Ovarian Tissue Grafts After Normo- and Hypothermic Storage

Viktoria Kiroshka,Galyna Semko,Volodymyr Pyatikop,Ahmed Gavas,Tetyana Bondarenko,Iryna Rula,Yulia Bozhkova

doi:10.15407/cryo31.04.326

Abstract

Morphological characteristics and function of ovarian tissue after 2–120 hrs of storage at 4 and 37°C in incubation media with different composition (mannitol-containing solution (MСS) and phosphate buffered saline (PBS)) were investigated. The function of ovarian tissue after normo- and hypothermic storage was studied by heterotopic transplantation to recipient animals simultaneously with bilateral ovariectomy. After 2-hour storage at 37ºC and 24-hour one at 4ºC in all the studied media, the estradiol and progesterone levels in plasma of recipient animals were experimentally proved to be 18.2–27.5 and 13.5–18.5 ng/ml, respectively, (after fresh tissue transplantation these values for estradiol and progesterone made (28.1 ± 3.6) pg/ml and (19.1 ± 2.8) ng/ml, respectively). Prolongation of tissue storage time at 37ºC increased the number of degenerative follicles, augmented TBARS concentration and reduced sex hormone levels after transplantation using both MCS and PBS. A protective effect of MCS only, manifested in endocrine function restoration in recipient animals after transplantation ((13.5 ± 2.5) pg/ml of estradiol) was found after 48 hrs of ovarian tissue hypothermic storage. If PBS was used, the estradiol level was within the indices for ovariectomized animals ((7.1 ± 1.5 (pg/ml)).

Highlights

Morphological characteristics and function of ovarian tissue after 2–120 hrs of storage at 4 and 37°C in incubation media with different composition (mannitol-containing solution (MСS) and phosphate buffered saline (PBS)) were investigated
Follicle morphology was slightly changed (Fig. 1B) and granulosa cells were somewhat compressed after 2-hour incubation of ovarian tissue fragments at 37°C in the solution I
A critical decrease of normal follicles within the ovarian tissue structure down to 30–35% was found after 6-hour normothermia and 72-hour hypothermia

Summary

Introduction

Morphological characteristics and function of ovarian tissue after 2–120 hrs of storage at 4 and 37°C in incubation media with different composition (mannitol-containing solution (MСS) and phosphate buffered saline (PBS)) were investigated. The main reason of organ and tissue ischemia is a partial or complete interruption of blood supply, initiating a cascade of such responses as a decrease in the level of macroergic compounds, disorder of enzyme kinetics, accumulation of decay. Чутливість органів (головний мозок, серце, легені, нирки, скелетні м’язи) до концентрації ТБК-активних продуктів визначається їх стійкістю до гіпоксії [1,2,3]. Що введення манітолу до складу реперфузійних середовищ зменшує зону некрозу в таких органах, як головний мозок [7, 17, 27], серце [24], нирки [9] та скелетні м’язи [29]. Реперфузія ішемізованої тканини яєчника 20%-м розчином манітолу [33] зменшувала ступінь ішемічного ураження і сприяла відновленню її функції

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Conclusion