Abstract
The article discusses some aspects of improving the elements of technology for creating of healthy collection of indigenous grape varieties in vitro. To recover from phytoplasma infec-tion, a chemotherapy method using the antibi-otic Cefotaxime has been developed. At high concentrations of the antibiotic, intoxication of plants and deterioration in their quality charac-teristics were noted. Plants of Makhrovatchik variety showed an improvement in survival rate at antibiotic concentrations of 100 and 200 mg/l: 64.3 and 60.7%, respectively. For long-term cultivation without replantings, the effectiveness of slowing down growth process-es by increasing the density of the nutrient me-dium was studied. The study was carried out on the varieties Makhrovatchik, Tsimlyansky Sergienko. Studied concentrations of agar-agar were (6, 8, 10, 12, 13 and 15 g/l). A decrease in the rate of growth processes was noted in all studied varieties, but the rate of the decrease was different.
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