Дослідження впливу аргініну глутамату на репаративні процеси у щурів з харчовою депривацією

Abstract

The purpose of the study was to experimentally substantiate the feasibility of arginine glutamate using, for the correction of reparative disorders in rats with partial food deprivation. Materials and methods. The work uses such research methods as modeling of food deprivation according to a patented method, modeling a wound defect of rat’s skin, evaluating the healing of wound defects by taking pictures with a camera with a resolution of 12 megapixels and subsequent processing with a special computer program Universal desktop ruler, version 11, for determining the area, determination of integral indicators of total protein, albumin, glucose, urea in blood serum of rats by spectrophotometric methods (“Filicit-diagnostics”). Statistical processing of the obtained results was carried out using non-parametric methods of analysis (MANOVA-test) using Statistica 6.0 software. Differences at p<0.05 were considered significant. Results and discussion. As a result of the food crisis, the share of hungry people in the world increases every year, and in 2022 it reached 9.8% of the world population. Starvation directly and indirectly prevents the successful healing of wounds, which is also complicated by impaired liver function. Partial food deprivation was accompanied by a violation of metabolic processes in the body of rats: a decrease in body weight (about 30%, p<0.05), a probable decrease in the content of glucose (by 24.5%), total protein and albumin (by 16.1 and 26.2%, respectively, p<0.05) with a simultaneous increase in the content of indicators of nitrogen metabolism in the blood. In addition, nutritionally deficient animals had a 4.7-day delay in wound healing compared to the control group. The use of arginine glutamate, in food-deprived rats caused a reduction in the wound skin defect and was accompanied by a partial normalization of metabolic processes: the total protein content and the amount of blood albumin were higher than those in the model pathology group without correction by 10.2 and 12.9%, respectively (p<0.05), the glucose content increased (+12.2%); simultaneously blood urea content was lower by 25.2% (p<0.05). Conclusion. The obtained data are an experimental basis for further clinical studies of the possibility of practical use of arginine glutamate in the complex treatment of patients with wound processes, especially under conditions of nutritional insufficiency