Уровень меди в молоке коров племенного хозяйства в зимний и весенний период

Abstract

As an essential test for all forms of life on earth, copper (Cu) is of great interest and a vast field for research. The inclusion of Cu in the functioning of a group of substances is carried out through amino acids. The scope of action of copper-containing proteins is quite wide. They are involved in the accumulation of blood cells and the functioning of the immune system, include increased lipid content, contraction of the cardiovascular system and body growth. The purpose of this work is to follow the dynamics of the Cu content in milk at the time of the season change and to establish the strength and direction of correlations with the amino acid composition of milk. In total, 88 samples of milk from the Ladoga breeding farm were studied in the work. Direct analysis in the Department of Physiology and Biochemistry of Farm Animals of the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst. In January, February, March and May, a random sample of 22 lactating cows was formed from the dairy herd. Milk samples were prepared for Cu analysis using the ETHOS EASY system (Italy), direct analysis was performed on a ZEEnit 650 P atomic absorption spectrometer (Analytik Jena AG). For the analysis of amino acids, an HPFA LC-20 AD Prominence system (Shimadzu, Japan) with an analytical module for post-column derivatization АРМ-1000 (Sevko&Co, Russia) is used. The highest values for Cu were set in March (149,4±7,6 μg/l), the lowest in May (79,9±4,2 μg/l). Significant differences with respect to January were established in May (p˂0,001) and March (p˂0,05). The most significant correlation coefficients in January – threonine, alanine, cysteine, tyrosine, phenylalanine, histidine, lysine; in February – aspartic and glutamic acids, threonine, serine, glycine, alanine, cysteine, valine, isoleucine, leucine, tyrosine, phenylalanine, histidine, lysine, proline; in March – aspartic and glutamic acids, threonine, serine, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, histidine, arginine.