Abstract
Dorsal root ganglia (DRG) are a potential source of neural stem cells, since they contain the neural crest-derived cells capable to dii¬€erentiate into neurons and dii¬€erent subpopulations of glial cells. It is known that under certain culture conditions, the DRG cells, derived from neonatal piglets form the i¬‚oating multicellular spheroids (MSs), capable to produce the i¬broblast-like cells (FLCs), glial cells (GCs) and neuron-like cells (NLCs). Here, we have cryopreserved the MSc, derived under serum-free culture conditions using 5, 7.5, 10% dimethyl sulfoxide (DMSO) and two-stage regimen of freezing. After cryopreservation, the MSs were established to preserve the capability to adhere as well as to produce three cell types: FLCs, GCs and NLCs. However, the capability of frozen-thawed MSs to produce dii¬€erent cell types was reduced. The cryoprotectant concentration ai¬€ected the cell type, prevailing in the culture, derived from the frozen-thawed MSs. After cryopresevation with 10% DMSO, the monolayer consisted mainly of FLCs, while with 5 and 7.5% DMSO it mostly comprised GLCs. Probl Cryobiol Cryomed 2019; 29(4): 354-364
Highlights
Dorsal root ganglia (DRG) are a potential source of neural stem cells, since they contain the neural crest-derived cells capable to differentiate into neurons and different subpopulations of glial cells
It is known that DRG comprises the bodies of sensitive neurons surrounding their Satellite glial cells (SGCs); Schwann cells, producing an axonal myelination; connective tissue cells of endoneuria [9]
The dimethyl sulfoxide (DMSO) concentration affects the cell type (GCs, neuron-like cells (NLCs) and fibroblast-like cells (FLCs)), which prevails in the culture after multicellular spheroids (MSs) thawing and transferring them under culture conditions
Summary
Dorsal root ganglia (DRG) are a potential source of neural stem cells, since they contain the neural crest-derived cells capable to differentiate into neurons and different subpopulations of glial cells. It is known that under certain culture conditions, the DRG cells, derived from neonatal piglets form the floating multicellular spheroids (MSs), capable to produce the fibroblast-like cells (FLCs), glial cells (GCs) and neuron-like cells (NLCs). The MSs were established to preserve the capability to adhere as well as to produce three cell types: FLCs, GCs and NLCs. the capability of frozen-thawed MSs to produce different cell types was reduced. The cryoprotectant concentration affected the cell type, prevailing in the culture, derived from the frozen-thawed MSs. After cryopresevation with 10% DMSO, the monolayer consisted mainly of FLCs, while with 5 and 7.5% DMSO it mostly comprised GLCs. Длительное сохранение жизнеспособной нервной ткани или клеток является современной и актуальной проблемой в связи с развитием методов лечения нейродегенеративных заболеваний и травм позвоночника. Единственный надежный способ долгосрочного хранения биологического материала, который позволяет не только сохранить его основные морфофункциональные характеристики, но и создать достаточный запас в криобанке [2]
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