ОСОБЛИВОСТІ ВНУТРІШНЬОЇ БУДОВИ ЯЄЧНИКІВ ПОТОМСТВА ЩУРІВ ПІСЛЯ ВВЕДЕННЯ ПРОГЕСТЕРОНУ ПІД ЧАС ВАГІТНОСТІ

Abstract

In obstetric and gynecological practice for miscarriage treatment the hormone progesterone is widely used.The aim of the study – to learn the correlation of ovarian structures of rats (follicles, connective tissue, blood vessels and corpus luteum) after the administration of progesterone during pregnancy.Materials and Methods. The ovaries of 48 female Wistar rats were investigated on the 45th, 60th and 90th days of life, and they were divided into 3 groups: intact, experimental (the oily solution of progesterone in a dosage 0.33 ml was administered intramuscularly to the female rats from the 15th to the 18th day of pregnancy), and the control group, which was injected with a physiological solution of sodium chloride in an equivalent dosage. The ovaries were fixed in 10 % neutral formalin, serial paraffin cross sections were stained with hematoxylin and eosin.Results and Discussion. From the 45th to the 90th day in the ovaries of experimental animals the connective tissue volume increase and the relative area occupied by the follicles decrease were observed comparing to the control group of animals. The relative area occupied by corpus luteum was significantly greater in the offspring of animals exposed to progesterone during pregnancy than in control animals. As for the blood vessels, there was a decrease in the relative area of vessels in the ovaries of experimental animals during the entire period of observation.Conclusions. In the experimental group of animals we observed the connective tissue and corpus luteum volume increase, but relative area occupied by follicles and blood vessels decreases during the entire observation period.