Abstract
The aim of research is to evaluate the nature of seasonal variations in the cortisol level in Romanov sheep in the conditions of Western Siberia. The concentration of cortisol was studied in winter and summer in purebred non-pregnant Romanov ewes aged 18 months, raised in Western Siberia. Blood serum samples were obtained from 26 sheep in winter and 34 sheep in summer. The level of cortisol in the blood serum was determined by solid-phase ELISA (reagent kit Steroid ELISA – cortisol, Alcor Bio, semi-automatic ELISA analyzer Thermo Scientific Multiskan FC). Generally accepted methods of descriptive statistics were used: the Anderson-Darling criterion was used to check the conformity of the distribution of cortisol values, the Fligner-Keelin criterion was used to assess the homogeneity of variances, and the Student's t-test was used for intergroup comparisons. The calculations were performed in the R-Studio data processing and graphics environment. The average values of cortisol in the blood serum in winter and summer periods in ewes were established, which were within the physiological norm.The level of cortisol in the summer period significantly exceeds the cortisol content in ewes in the winter period by 117.2 nmol/l (p < 0.05). For Romanov sheep living in Western Siberia, reference intervals for the concentration of cortisol in the blood serum were established: in the winter from 10.5 to 122.3 nmol/l, and in the summer – from 78.5 to 286.7 nmol/l.
Published Version
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