Дослідження впливу чотирьох генеричних пестицидів лямбда-цигалотрину на репродуктивну функцію самців щурів wistar han

Abstract

Introduction. Objective is to identify hazard and asses reproductive toxicity risk of four generic lambda-cyhalothrin pesticides of different purity on male Wistar Han rats. Methods. Lambda-cyhalothrin (LC1 — 97 %, LC2 — 96 %, LC3 — 97,1 %, LC4 — 96,7 % purity) was administered intragastrically on a daily basis, exceptfor Saturday and Sunday, at doses of 0,0; 0,3 and 3,0 mg/kg body weight for three groups of animals, 20 males in each, for 11 weeks. Control animals received an equivalent amount of solvent. In parallel with control and experimental animals, there were intact females, intended for mating. At the end of inoculation, functional parameters of the state of gonads and the ability of animals to reproduce offspring were studied. The state of reproductive function in intact females was taken into account at day 20 of pregnancy. Results and discussion. The obtained data indicate that the exposure to four generic lambda-cyhalothrin substances at a dose of 3,0 mg/kg body weight for 11 weeks showed a general toxic effect (weight loss) in males under the effect of LC1. All four test substances have a reproductive toxicity at the maximum dose and have a pronounced antiandrogenic effect, which is manifested in changes in the morphological and functional parameters of the state of the gonads in the experimental groups of males. In addition, there was a negative effect on the fertility of males (LC2), which was concluded by the fertility index in intact females. Conclusion. Based on the results obtained, it can be concluded that all studied lambda-cyhalothrin samples have reproductive toxicity at a dose of 3,0 mg/kg body weight. No observed effect level (NOEL) for all studies substances is the dose of 0,3 mg/kg body weight. In the range of studied doses, there is a dose-effect dependence. Key words: pesticides, cyhalothrin, lambda-cyhalothrin, gonadotoxicity, reproductive toxicity.