Abstract

We discuss results of experiments evaluating effect of fixing solution on the body weight of three species of amphipods, Locustogammarus locustoides (Brandt, 1851), Orchestia ochotensis Brandt, 1851 and Parhyale zibellina Derzhavin, 1937, in order to estimate the degree of influence of 70% ethanol solution on the weight of crustaceans during long-term storage. As a result, we found that during the period of conservation lasting 16 years and 3 months, the average body weight of individuals of the first two species decreased by 21% and 26%, respectively in comparison to the initial weight. In case of P. zibellina, 18% decrease in body weight occurred after 13 years of storage. The need to consider effect of alcohol-containing solutions on the body weight of amphipods is discussed. This is particularly important in studies where weight values are used as input or intermediate data for subsequent calculations. These may include estimation of biological production, determination of energy metabolism rates and modeling of growth processes and etc. It is proposed to use correction coefficients to restore the values of the primary mass of amphipods that were stored in a fixed form.

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