Abstract
The article presents the dynamics of changes in female fertility of I, II and III generations of rats and morph functional state of rats uterus of I generation, which were fed with feed containing 20% traditional and genetically modified soy. Received three generations of rats that consumed feed containing 20% of GM and traditional soybeans. It was received generation of rats that consumed feed containing 20% of GM and traditional soybeans. The reproductive ability was evaluated by the fertility of animals. On 135 day of life 5 rat females from each group (60 days after birth) were taken out of the experiment through ether anesthesia, were decapitated, the uterus were taken for histological and histochemical research, which were fixed in 10% solution of neutral formalin, Karnua liquid, followed by pouring into paraffin. It was produced histoslices, stained with hematoxylin-eosin, by McManus combined with altsianic blue.It was established that long-term feeding of feed containing 20% GM soya negatively affected the 1 generation of animals, as expressed lowering of reproductive capacity of female rats and tendentious decrease in the number of heads of newborns in the young rat nests. In the next two generations of female rats of II and III groups probable differences in the investigated indices were not found which is probably due to the inclusion of adaptive compensatory mechanisms to change feeding ration.For histological examination of the rats uterus of the I generation, who consumed feed containing 20% soybean was set to reduce the secretion of glycans replacement, which has reduced barrier protection, damage of epithelial cells, development of atrophic processes in the myometrium and endometrium, cyst like extension of one uterine glands and vacuolic epithelial dystrophy of others, lymphoid-histiocytic infiltration of the lamina propria of the endometrium, indicating that the decline in morphological functional state of the uterus.
Highlights
Матеріал і методи дослідженьБуло сформовано 3 групи тварин, по 14 щурів у кожній (8 самок і 6 самців).
I група – контрольна, які одержували стандартний корм віварію.
II група – щурам згодовували корм з додаванням 20% традиційної сої сорту «Аннушка».
Summary
Було сформовано 3 групи тварин, по 14 щурів у кожній (8 самок і 6 самців). I група – контрольна, які одержували стандартний корм віварію. II група – щурам згодовували корм з додаванням 20% традиційної сої сорту «Аннушка». IІІ група – тварини отримували корм з додаванням 20% генномодифікованої сої (сорт «Roundap Ready» лінії 40–3–2, який містить трансгени cp4epsps та регуляторні елементи – промотор E35S і термінатор NOS). Які споживали корм з 20% вмістом ГМ і традиційної сої. Для оцінки постнатального розвитку приплоду визначали кількість народжених щуренят, мертвонароджених та здатністю до виживання у перші два місяці. У тварин відбирали кров, по 5 щурематок з кожної групи (60 добу після родів) виводили з експерименту шляхом ефірного наркозу, декапітували, проводили автопсію. Мікрофотографування гістопрепаратів здійснювали за допомогою мікроскопа OLYMPUS CX 41 та фотокамери OLYMPUS C-5050
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
