Abstract
The results of experimental investigation of autoimmune reaction targeted against neirospecific protein antigens in patients with congenital heart failure are presented. It is shown that titer for autoanibodies to these patients’ brain proteins is significantly higher compared with healthy persons. Elevation of titer of these autoantibodies is correlated with the ability to solve tasks for revealing complex analogies and decrease of life quality. Therefore, worsening of life quality and development of cognitive deficit in patients with cardiovascular diseases might be associated with autoimmune reaction against specific proteins of nervous tissue cells. The results obtained demonstrate that generation of autoantibodies in patients with congenital heart failure is linked with the cognitive deficit. One of the most important causes of cognitive decline could be hypoxia state due to surgery intervention. Consequences of hypoxia, in their turn, are accompanied by chronic oxidative stress. Molecular and cell injuries induced by free radicals are known to be the widely spread cause of various pathologies. Further investigations for validation of diagnostic hallmarks for estimation of the origin of progression of the cognitive deficit in patients with congenital heart failure are needed. These studies will allow clarifying mechanisms of influence of hypoxia-induced injuries associated with the aggravation of oxidative stress in nervous tissue cells. Understanding of relationship between decline of cognitive function and pathologies of cardiovascular system is necessary for more precise clinical diagnostics of the early prevention of possible complications and proper treatment of patients.
Highlights
Представлено результати експериментальних досліджень аутоімунної реакції, спрямованої проти нейроспецифічних білкових антигенів у пацієнтів з уродженою вадою серця
Elevation of titer of these autoantibodies is correlated with the ability to solve tasks for revealing complex analogies and decrease of life quality
Worsening of life quality and development of cognitive deficit in patients with cardiovascular diseases might be associated with autoimmune reaction against specific proteins of nervous tissue cells
Summary
У зразках сироватки методом імуноблотингу визначали присутність аутоантитіл. Відповідні фракції, збагачені білками клітин мозку, одержували як описано раніше (Nedzvetsky et al, 2006; Baydas et al, 2003). Імуноблотинг із моноспецифічними антитілами проти гліального фібрилярного кислого білка (ГФКБ) проводили як це описано раніше (Nedzvetsky et al, 2006). Отримані результати сканували та аналізували за допомогою комп’ютерної програми AlfaImager 2000. Для кожної сироватки пацієнтів із ВВС визначали максимальне розведення, за якого інтенсивність забарвлення основних поліпептидних зон була не меншою за умовну одиницю. Всі проби білків мозку містили однакову кількість загального білка (30 мкг). Вміст загального білка у пробах визначали методом Лоурі в модифікації Міллера (Miller, 1959). Отримані дані обробляли з використанням стандартних методів математичної статистики (пакет програм Statistica 6.0)
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