Abstract
The article presents data on the testing of an indirect method of DNA extraction from soil to identify the soil microbiota of leached chernozems based on the analysis of the 16s rRNA gene. For this method, the optimal amount of soil sample was set at 0.3 - 0.4 g. Isolation of DNA from samples depending on the depth of the layer showed that the highest concentration was obtained from samples from depths of 10-20, 20-30 cm. As a result of the analysis, preparations of total DNA were obtained in various concentrations and different optical purity.
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Schedule a callMore From: Scientific Works of North Caucasian Federal Scientific Center of Horticulture Viticulture Wine-making
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