ДОСЛІДЖЕННЯ ПРОТЕОЛІТИЧНОЇ АКТИВНОСТІ В НИРКАХ ТА ПЕЧІНЦІ ЩУРІВ ЗА РОЗВИТКУ ХРОНІЧНОЇ АЛКОГОЛЬНОЇ ІНТОКСИКАЦІЇ

Abstract

The study of biochemical processes in chronic alcohol intoxication is a topical issue of our time. A deeper understanding of the mechanism of action of proteinases in this pathological condition will help in the development and search for non-invasive diagnostic methods, thereby minimizing the risk of harming human health during complex diagnostic procedures. In our experiment, we investigated the general proteolytic activity, the activity of metalloproteinases and serine proteinases in the liver and kidneys of rats on an experimental model of chronic alcohol intoxication on days 1, 3, 7, and 11 of the experiment, as well as on days 21 and 28 after the cessation of ethanol administration. Male rats weighing 180–200 g were modeled for chronic alcohol intoxication by intragastric administration of 30% ethyl alcohol solution for 10 days on an empty stomach, at the rate of 2 ml per 100 g of animal weight. Liver and kidney homogenate by well-known methods. The concentration was determined by the Bradford method. The total proteolytic activity, the activity of metalloproteinases and serine proteinases were determined by the caseinolytic method with modifications. The total proteolytic activity, the activity of metalloproteinases and serine proteinases were determined by the caseinolytic method with modifications. It was shown that on the 3rd, 7th and 11th days of the experiment in the liver there was an increase in the total proteolytic activity and the activity of metalloproteinases. The activity of serine proteinases significantly increased on days 3 and 7 of the study. In the kidneys, a significant increase in all studied activities was observed only on the 3rd day. Such differences in the activities of metalloproteinases and serine proteinases can be associated with the different roles of these enzymes in physiological processes. Thus, we observed an increase in the activity of serine proteinases in acute intoxication, and in metalloproteinases in chronic intoxication.