Abstract
Thin-layer chromatography (TLC) has been used to study the influence of a number of factors (the nature of the stationary phase, the nature and concentration of the organic solvents and surfactants, the pH of the buffer solution) on the chromatographic characteristics of B vitamins: B 1 (thiamine), B6 (pyridoxine), B12 (cyanocobalamin). The stationary phases were plates with polar (Sorbfil on a polymer (highperformance plates, HTLC) and aluminum substrates), weakly polar (Polyamide-6), and nonpolar (RP-18 on an aluminum substrate) sorbents. As micellar mobile phases – micellar solutions of cetylpyridinium chloride in the concentration range 5–10-4 – 5–10-1 M, sodium dodecyl sulfate in the concentration range 2,5–10-4 – 5–10-1 М. It has been established that among aqueous-organic mobile phases (MF) the best results have been obtained for MF of the composition acetonitrile – water (15:385) on polar high-performance plates. More effective than acetonitrile – water is the PF of the composition acetonitrile – cetylpyridinium chloride (15:85) in the presence of a phosphate buffer solution with pH = 3. For the last PF, TLC determination of vitamins B1, B6, B12 in pharmaceutical preparations with a Sr value of interval 0.01–0.06 has been developed.
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