Abstract
The impact of protective media supplemented with sucrose, glycerol, and maltose on the rabies virus CVS strain preservation during storage at 37, 5, –20, –80, and –196ºC has been studied. The temperature regimen of 37ºC was found as not suitable for virus storage, but temperature of 5ºC could be used for a short-term storage in corresponding protective medium in case of production need. It was shown that storage at –80 and –196ºC in DMEM-based preservation media containing 0.5% fetal bovine serum, supplemented with either 5% sucrose, or 5% glycerol, or 5% maltose, or sucrose and glycerol mixture provided high levels of the rabies virus CVS strain infectious activity within 24 months (observation period). At –20ºC, in a growth medium with sucrose and mixture of sucrose and glycerol, after 6 months 79–82% of the initial virus activity were preserved, and after 12 months that was 69–72%. Upon reaching –80ºC and during subsequent storage of virus samples at this temperature in the studied preservation media, the secondary crystallization of concentrated dissolved substances was prolonged. Probl Cryobiol Cryomed 2020; 30(2): 148–157
Highlights
The impact of protective media supplemented with sucrose, glycerol, and maltose on the rabies virus CVS strain preservation during storage at 37, 5, –20, –80, and –196oC has been studied
Under storage at 5oC for 3 months, the highest rate of virus preservation was observed in the additivefree growth medium (GM): 22% of the initial infectious activity, after 6 months the virus was inactivated in all the samples except the medium with sucrose and glycerol mixture, where 13% of the initial index was preserved
The storage temperatures of –80 and –196°C and DMEM-based protective media were established to provide a high infectious activity of the rabies virus CVS strain for 24 months
Summary
The impact of protective media supplemented with sucrose, glycerol, and maltose on the rabies virus CVS strain preservation during storage at 37, 5, –20, –80, and –196oC has been studied. It was shown that storage at –80 and –196oC in DMEM-based preservation media containing 0.5% fetal bovine serum, supplemented with either 5% sucrose, or 5% glycerol, or 5% maltose, or sucrose and glycerol mixture provided high levels of the rabies virus CVS strain infectious activity within 24 months (observation period). Якість антирабічних препаратів у ході виробництва можна забезпечити лише за наявності достатніх запасів вірусу сказу штаму CVS, отри-. The quality of rabies products in manufacturing may be only ensured when sufficient stocks of the rabies virus CVS strain, obtained from a single batch is available.
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