Вплив одноразового перорального введення щурам асоціату таурин-декстрин на концентрацію амінокислот у плазмі крові

Abstract

The aim of the study was to test the ability of dextrin particles of different sizes, modified with N-stearylglutamic acid, to increase the transport of taurine into the blood plasma of rats. To achieve this goal, Wistar rats weighing 240–260 g were divided into control and four experimental groups. The control group was administered once perorally with water. The first experimental group was administered perorally with an aqueous solution of taurine at a dose of 100 mg/kg, the second and third ― a solution of taurine-dextrin associate with particle sizes of 60–90 nm and 500–800 nm, in which the dose of taurine was 100 mg/kg. The fourth experimental group (IV) was administered only dextrin. In 30 min, the rats were decapitated, and blood was collected. Blood cells and proteins were precipitated, and samples were derivatized with 1-fluoro-2,4-dinitrobenzene. Free amino acids of plasma were separated on a Dionex Ultimate 3000 liquid chromatograph equipped with a spectrophotometric detector, chromatographic column Luna C18 (2) 250×4.6 mm, elution type was gradient. It was registered that in the first and third experimental groups the content of taurine in the blood plasma of rats was 33.8% higher than in the control. Oral administration of taurine-dextrin associate with 60–90 nm particles increases the taurine content by 62.6% compared to the control and by 21.6% more than in the first and third experimental groups. This indicates efficient transport of taurine by dextrin only with particles of 60–90 nm. Administration of both taurine and taurine-dextrin associate reduced concentrations of glutamine, methionine, lysine, alanine, phenylalanine, and histidine. Moreover, the reason for the decrease in the content of the last two amino acids was not only taurine, but also the transporter itself. With the administration of the transporter the concentration of asparagine was lower than in control, and the content of arginine, on the contrary, higher. In the first experimental group, the concentration of aspartic acid and serine decreased, and in the second ― valine, leucine and tryptophan, in the third ― glutamic acid. The glycine content increased in the second experimental group. Ornithine content in all experimental groups except the third was higher, compared with the control. Thus, modified dextrin with a particle size of 60–90 nm increases the transport of taurine into the blood.