Abstract
The nucleic acids amplification is one of the key methods for molecular biology research and clinical diagnostics. The isothermal amplification methods, for example rolling circle amplification, are a good alternative for a widely spread polymerase chain reaction. Strand-displacement DNA polymerases are required for isothermal amplification. In this work, we studied the influence of temperature on the formation of specific and non-specific amplification products by 9°Nm, Vent exo-, Hemo KlenTaq DNA polymerases during rolling circle amplification. The temperature values for the most effective formation of non-specific products and specific concatemeric products were determined. The obtained data will allow the development of more specific isothermal amplification methods with DNA polymerases used.
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