Abstract
In this research, we have studied the impact of electrolytes and Ca2+ ions on the development of hemolytic damages in human erythrocytes in cryoprotectant solutions under mechanical stress, as well as the ei¬€ect of freeze-thawing of cells in the presence of glycerol and polyethylene glycol (PEG) on their mechanical stability. The decrease in mechanical stability of cells was found when the salt concentration in extracellular medium increased. At the same time, the absence of electrolytes in cryoprotectant solutions reduced their stability under mechanical stress as well. The Ca2+ introduction into the media increased the cell hemolysis only in the presence of PEG, but not glycerol, apparently due to dii¬€ erent ei¬€ects of these substances on the activity of Ca2+-regulating systems. The introduction of salt of ethylenediaminetetraacetic acid (EDTA) into the media composition reduced the erythrocyte membrane mechanical stability in the presence of the both cryoprotectants, which might be due to the chelator ei¬€ect on membrane-bound Ca2+. Cryopreservation of erythrocytes increased their sensitivity to mechanical stress, and even the cryoprotectant removal could not restore the cell properties up to the control parameters. Probl Cryobiol Cryomed 2019; 29(4): 317-331
Highlights
In this research, we have studied the impact of electrolytes and Ca2+ ions on the development of hemolytic damages in human erythrocytes in cryoprotectant solutions under mechanical stress, as well as the effect of freeze-thawing of cells in the presence of glycerol and polyethylene glycol (PEG) on their mechanical stability
The effect of high electrolyte concentrations on erythrocyte membrane mechanical stability during cryopreservation can be studied in model experiments with mechanical stress
The exceeding of NaCl physiological range was found to increase the membrane sensitivity to stress, and the hemolytic damages of erythrocytes augmented with increasing salt concentration and stress exposure duration (Fig. 1)
Summary
We have studied the impact of electrolytes and Ca2+ ions on the development of hemolytic damages in human erythrocytes in cryoprotectant solutions under mechanical stress, as well as the effect of freeze-thawing of cells in the presence of glycerol and polyethylene glycol (PEG) on their mechanical stability. Shifts in salt concentration during water crystallization and thawing [17, 20] or the alterations in medium osmolarity when introducing/removing the hypertonic solutions of cryoprotective agents (CPAs) are accompanied by changes in cell volume, while the membranes pass through mechanical.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
