Особенности проявления постгипертонического лизиса эритроцитов некоторых млекопитающих

Elena A Semionova,Nataliya M Shpakova,Nataliya A Yershova,Nataliya V Orlova,Sergey S Yershov

doi:10.15407/cryo26.01.073

Abstract

The erythrocyte sensitivity of some mammalian species (human, rat, rabbit) to posthypertonic lysis effect when varying NaCl concentration in dehydration medium, cell incubation duration and temperature was studied. We demonstrated here a determining role of dehydration stage (compared to rehydration one) for posthypertonic lysis development in mammalian erythrocytes, since the salt concentration in hypertonic medium and incubation duration in it determined the level of cell hemolysis. The loss of potassium cations by human and rabbit cells, depending on salt concentration in the medium was revealed at dehydration stage. The level of posthypertonic lysis in human, rat and rabbit erythrocytes was established as determined by experimental temperature conditions. Values of posthypertonic lysis of mammalian erythrocytes are lower at0°C, than at37°C, and this feature is much pronounced for animal cells. The results of comparative analysis of cell response to posthypertonic lysis effect showed rabbit erythrocytes to have the maximum resistance, and the minimum one was in rat cells. Probl Cryobiol Cryomed 2016; 26(1):73-83.

Highlights

The erythrocyte sensitivity of some mammalian species to posthypertonic lysis effect when varying NaCl concentration in dehydration medium, cell incubation duration and temperature was studied
In order to evaluate the contribution of the processes, developing at both PHL stages, we varied the time of mammalian erythrocyte staying in hypertonic and isotonic media (Fig. 1 and 2, respectively)
Analysis of the findings suggested the dependence of human erythrocyte PHL on the osmolality of dehydration medium and the temperature, but in lesser extent

Summary

Introduction

The erythrocyte sensitivity of some mammalian species (human, rat, rabbit) to posthypertonic lysis effect when varying NaCl concentration in dehydration medium, cell incubation duration and temperature was studied. We demonstrated here a determining role of dehydration stage (compared to rehydration one) for posthypertonic lysis development in mammalian erythrocytes, since the salt concentration in hypertonic medium and incubation duration in it determined the level of cell hemolysis. Для изучения постгипертонического лизиса клеток на этапе отогрева используют разные модельные подходы. В основе ПГЛ клеток лежат два процесса: дегидратация при инкубировании в гипертонической среде и последующая регидратация, которая развивается при переносе клеток в изотонические условия, однако для гипотонического лизиса характерен только один процесс, связанный с набуханием клеток при их переносе в гипотонические условия. Поэтому для моделирования действия на клетки факторов криоповреждения в условиях отогрева клеточной суспензии лучше использовать ПГЛ

Methods

Results

Conclusion