ИССЛЕДОВАНИЕ АДСОРБЦИИ АМФОТЕРНОГО ПАВ АМИНОКСИДА НА МИКРОБНЫХ КЛЕТКАХ С ЦЕЛЬЮ ВЫДЕЛЕНИЯ ВЕЩЕСТВ БЕЛКОВОЙ ПРИРОДЫ

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A promising direction in obtaining effective forages is the creation of technology for obtaining protein from microbial biomass and providing livestock breeding with new types of protein-vitamin concentrates. The Acetobacter methylicum strain, a producer of fodder protein, was used as an object of research. One of the stages of microbial protein production - the stage of isolation of microbial synthesis products - was studied. Protein isolation was carried out at the stationary phase of growth, the time of reaching which was determined by the experimental growth curve of bacterial culture. To intensify the stage of obtaining the final product in this study it is proposed to use an ampholytic surfactant - aminoxide (alkyldimethylamine oxide). It was found that at the concentration of surfactant in the culture liquid up to 1% adsorption of surfactant on the cell walls of bacteria takes place, while there is a destruction of cell walls and, accordingly, a decrease in the number of viable cells in the population of Acetobacter methylicum, which is probably associated with changes in the permeability of cell walls and their subsequent lysis. As a result, the number of viable cells decreases and the amount of released protein increases, which was monitored by the PI parameter (percentage of cell viability in the population). It was shown that for this surfactant the optimal concentration for cell destruction and protein release is 0.5% and the incubation time with surfactant is enough 15 minutes. Changing the surfactant concentration does not result in significant protein yield.