Відповідь ізольованих клітин букального епітелію людини на спільну дію протипухлинного антибіотика доксорубіцину і магнітного поля

Abstract

The combined effect of doxorubicin and magnetic field on the viability of isolated buccal epithelial cells two donors was investigated. Doxorubicin is an effective antitumor antibiotic, but this drug has a large amount of side effects. This significantly reduces the number of cases when you can change the drug. To reduce the toxic effect or to increase the effectiveness of anticancer therapy, the introduction of additional substances is used. For a long time, magnetic and electromagnetic fields have been used as a therapeutic factor by three therapies for cancer alone, or as an adjunct. Doxorubicin at a concentration of 2 μg/ml for 2 hours has been shown to increase of cell membrane permeability and heterochromatin granule quantity in cell nuclei. Exposure of cells to 25 mT magnetic field for 30 and 60 minutes results in decrease in cell viability and increase in cell membrane permeability and the number of heterochromatin granules in nuclei. Combined exposure to static magnetic field and doxorubicin results in decrease of the number of heterochromatin granules in the nuclei of cells from one of the donors, as compared to the variant of exposure only to doxorubicin, which indicates the protective effect of the magnetic field. A possible mechanism of the protective action of the magnetic field in our experiments is that the stress under the influence of the magnetic field activates the protective mechanisms of the cell (including the transition of chromatin to a heterochromatinized state). That way, been pre-activated due to the action of the magnetic field, the defense mechanisms of the cell reduce the toxic effects caused by doxorubicin.