Первичный скрининг бактериальных штаммов антагонистов из коллекции лаборатории биометода ВНИИМК к возбудителю фомоза подсолнечника (часть 2)

Abstract

There are presented data of the primary screening of bacterial strains antagonists from a collection of the biological methods lab of the V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK) to an aggressive isolate of the Phoma rot pathogen (Phoma macdonaldii Boerema) affecting sunflower by a method of double or crops. We estimated 27 bacterial strains of Bacillus genus on the three nutrient mediums (potato-sucrose agar (PSA), Taylon-3 and oat agar (OA). We stated 21 of them possessing antagonistic activity with a type of action mode against Phoma pathogen on sunflower named antibiosis. We determined for three strains presence of a competition for nutrient area without formation of a sterile zone with pathogen. Maximal sterile zone to Phoma rot pathogen was formed by strains: 11-3 Bacillus sp. (21.0 mm) on PSA; B-4 B. circulans (19.0 mm) on a Taylon-3 medium and D 1-1 Bacillus sp. (17.0 mm) on OA. Eight from the nine collection bacterial strains of Pseudomonas genus demonstrated antagonistic activity to Phoma rot on PSA and King’s B, and seven strains – on OA with one type of action mode – antibiosis. Maximal sterile zone to Phoma rot pathogen was formed by strains: Oif 2-1 Pseudomonas sp., 16-2 Pseudomonas sp. and 12-2 Pseudomonas sp. (12.5– 12.0 mm) – on PSA; 16-2 Pseudomonas sp. (16.5 mm) – on King’s B; Oif 2-1 Pseudomonas sp. (18.0 mm) – on ОА.