Antimycotic activity of bacterial strains against the pathogen of grape necrotic leaf spotting Alternaria sp.

Margarita Burovinskaya,Eugeniya Yurchenko,Lyubov Maslienko

doi:10.1051/e3sconf/202125405004

Margarita Burovinskaya, Eugeniya Yurchenko + Show 1 more

Open Access

https://doi.org/10.1051/e3sconf/202125405004

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Abstract

Species of the genera Bacillus Cohn and Pseudomonas Migula are successfully used as biocontrol agents for many mycopathogens, including Alternaria sp. To assess the bacteria antagonistic potential against Alternaria sp, we used the method of counter cultures on various nutrient media – universal and specialized. Of the 24 bacterial strains of the genus Bacillus, 18 strains were developed antagonistic activity to Alternaria sp. after 10 days of co-cultivation on the Tylona-3 medium. The maximum sterile zone (8.0 mm) was formed by bacterial strains (R-9, 5B-1, 01 cor f Bacillus sp.). 3 strains of 9 bacteria of the genus Pseudomonas, developed antagonistic activity to Alternaria sp. on Kinga B medium - 14-3 Pseudomonas sp., Oif 2-1 Pseudomonas sp., 14-4 Pseudomonas sp. The maximum antibiotic activity on potato-sucrose agar was established in strains 3-3 Bacillus sp., and K 1-1 Bacillus sp. (sterile zone 7.5 mm). All the tested bacteria most often caused one sign of antagonism – antibiosis.

Highlights

Alternaria Nees species are mainly saprotrophic fungi
The process of plant infection with Alternaria fungi includes the germination of conidia, penetration and colonization of the plant surface
The results showed that volatile organic compounds (VOCs) and unstable lipopeptides showed significant antagonistic activity against A. solani, with a decrease in colony diameter and significant inhibition of conidia germination [19]

Summary

Introduction

Alternaria Nees species are mainly saprotrophic fungi. some species have acquired the ability to cause diseases of a wide range of host plants [1]. The process of plant infection with Alternaria fungi includes the germination of conidia, penetration and colonization of the plant surface. The site of pathogen penetration is surrounded by necrotic and often chlorotic halos Such tissue damage is caused by the diffusion of secondary fungal metabolites – toxins. The authors of the published works provide signs of grape infection with Alternaria blight with reference to the only source F. When studying the Alternaria blight of grapes in the Krasnodar Krai, the species A. tenuissima was noted as the causative agent, while the signs of the disease were radically different from those listed above. According to the results of Aldib et al P. brassicacearum and P. jessenii strains effectively suppressed the potato pathogen Alternaria solani [17]. SEB1 secretes thermally stable antimycotic metabolites [20]

Materials and methods

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Conclusion