Abstract
In order to optimize the productivity of plant cell cultures for pharmacologically valuable compounds(steroidal glycosides), the effect of the elevated concentrations copper ions on growth and biosynthetic characteristics of the suspension culture of Tribulus terrestris L. has been studied. It was shown that the increase in the content of copper ions in nutrient medium by 5-100 times (up to 10 uM) did not practically affect growth characteristics of the cell culture; however, high concentrations (5 and 10 uM) caused a significant (below 50%) decrease in the cell viability. It was proved by UPLC ESI MS that the T. terrestris biomass contains a furostanol glycoside, terrestrinin B, and its 25(R)-stereoisomer. An increase in the concentration of copper ions in the medium provided a significant (up to 3 times) increase in the content of furostanol glycosides in the first cultivation cycle, and this compound was maintained in the next 5-7 cultivation cycles, although its content was 1.5-2 times lower. On the contrary, changes in the hormonal status of the medium (removal of α-NAA) could result in changes in the composition of furostanol glycosides (disappearance or a drastic decrease in the content of terrestrozin N derivatives, the presence of which in this plant cell culture was shown previously). It may be concluded that heavy metals (copper ions) can intensify the formation of steroidal glycosides in T. terrestris cell culture, whereas growth regulators (for example, auxins) can change their qualitative composition. copper ions, steroidal glycosides, plant suspension cell culture, Tribulus terrestris The work was financially supported by the Russian Science Foundation (№ 16-14-00126).
Published Version
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