Abstract
We studied the effect of different levels of membrane-bound hemoglobin on the level of red-cell membrane proteins and also their interrelation in patients with essential hypertension with and without metabolic syndrome. It was found that high membrane-bound hemoglobin is closely related to the low level of high-density lipoproteins and high level of low-density lipoproteins in patients with essential hypertension complicated with metabolic syndrome. In patients with essential hypertension not complicated with metabolic syndrome high membrane-bound hemoglobin is related to the increased prothrombin time and decreased blood urea nitrogen. In patients with essential hypertension com-plicated with metabolic syndrome high membrane-bound hemoglobin significantly influences the level of membrane contractile proteins (actin, tropomiosine). In patients with essential hypertension without metabolic syndrome high membrane-bound hemoglobin is accompanied by the decrease of structural and integral membrane proteins levels (anion-transport protein and protein 4.1). As the result of quantitative changes in these proteins and change in their interrelations in patients with ssential hypertension complicated with metabolic syndrome more intensive disorders of structural and functional organization of red-cell membrane can appear.
Highlights
We studied the effect of different levels of membrane-bound hemoglobin on the level of red-cell membrane proteins and their interrelation in patients with essential hypertension with and without metabolic syndrome
It was found that high membrane-bound hemoglobin is closely related to the low level of high-density lipoproteins and high level of low-density lipoproteins in patients with essential hypertension complicated with metabolic syndrome
In patients with essential hypertension not complicated with metabolic syndrome high membrane-bound hemoglobin is related to the increased prothrombin time and decreased blood urea nitrogen
Summary
В исследовании принимал участие 51 мужчина с ЭАГ I–II степени. Средний возраст больных – 42 ± 1,5 лет. Диагноз ЭАГ и дифференциальная диагностика для исключения симптоматической артериальной гипертонии проводились в соответствии с рекомендациями ВНОК (2008). У больных ЭАГ определяли содержание основных белков мембраны эритроцитов и мембраносвязанного гемоглобина. С помощью автоматического биохимического анализатора «Синхрон-9» фирмы Bekman (США) изучали комплекс биохимических параметров крови (липидный спектр, уровень мочевины, креатинина, мочевой кислоты, С-реактивного белка и глюкозы). Концентрацию белков определяли с использованием набора Qubit Protein Assay Kit (Invitrogen, США) на приборе Gubit Protein, согласно инструкции фирмы-изготовителя. В результате исследования 205 электрофореграмм белкового спектра была проведена количественная оценка 10 мембранных белков эритроцитов: α-спектрина, β-спектрина, анкирина (полоса 2.1), анион-транспортного белка (АТБ), белка полосы 4.1, трансмиттера глюкозы (GLUT), актина, глицеральдегид-3-фосфатдегидрогеназы (Г-3-ФДГ), тропомиозина, глутатион-S-трансферазы (Гл.-S-Тр.). Определение МСГ в структурных фрагментах мембраны эритроцитов проводили в гемолизатах по убыли гемоглобина до и после центрифугирования при 6000 об/мин.
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