Abstract
With the aim of developing a culture method for efficient plant regeneration in vitro, the aseptically treated seeds of Lolium perenne L., Linum grandiflorum L., Brachycome iberidifolia L., Chrysanthemum carinatum L. and Trifolium repens L. were cultured on B 5 and Murashige and Skoog (MS) media, containing different combinations of plant growth regulators to induce a callus. The highest percentage of calluses of Linum grandiflorum was observed on В 5 with 2,4-D (6 mg/l) and МS with 2,4-D (6 mg/l) and kinetin (2 mg/l). In Lolium perenne L., MS with 2,4-D (4 mg/l) was used for the best callus formation. In Brachycome iberidifolia, В 5 with 2,4-D (4 mg/l) and kinetin (2 mg/l) or B 5 with 2,4-D (6 mg/l) and kinetin (2 mg/l) were the most effective conbinations. The best callus formstion for Chrysanthemum carinatum and Trifolium repens were achieved on MS supplemented with 6-BAP (1 mg/l) and IAA (0,1 1 mg/l) and В 5 enriched with 2,4-Д (8 mg/l) and kinetin (2 mg/l), respectively. MS free of phytphormones, B 5 with 6-BAP (1 mg/l) and NAA (0,1 mg/l), and 1/ 2MS with 6-BAP (2 mg/l) and NAA (0,1 mg/l) were identified as the optimal combination for plant regeneration for Lolium perenne L., Linum grandiflorum and Brachycome iberidifolia, respectively.
Published Version
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