Abstract
PURPOSE OF THE ARTICLE. In the past decades a number of extraction methods for biologically active compounds of ginseng coupled with different detection techniques were created. For HPLC-MS profiling of these compounds in plant material and related products a fast non-destructive way to extract ginsenosides from plant materials and ginseng products based on ultrasound-assisted extraction was developed. FINDINGS. Calculated by means of "Added-Found" approach recoveries of ginsenosides Rg1, Rb1, Rc from ginseng root and model sample known to be free of analytes ranged from 80 to 110 %. The effect on the recovery of the following factors: the composition of the extracting mixture, the volume of the mixture and the number of successive extractions was studied. The extraction in case of using 50-70 % methanol/ethanol water mixtures was 1.5-2 times more effective than with 10-20 % mixtures. CONCLUSIONS. After optimization developed method of extraction without subsequent evaporation and reconstitution of the extract was used in combination with HPLC-MS determination of ginsenosides in various objects with a complex matrix. Keywords : ultrasound-assisted extraction, ginsenosides, Panax ginseng , HPLC- MS/MS (Russian) DOI: http://dx.doi.org/10.15826/analitika.2013.17.4.012 A.N. Stavrianidi, I.A. Rodin, A.V. Braun, O.A. Shpigun Lomonosov Moscow State University , Moscow, Russian Federation
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