Вплив препаратів «Бровермектин-грануляттм» і «Авесстимтм» на рівень продуктів ПОЛ та активність ферментів АОС у гепатопанкреасі однорічок білого амура, уражених моногенеями

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The data on the influence of the anti-parasitic drug «Granulated brovermectin» and immunomodulator «Avesstim» on the level of lipid oxidation products (LOPs) and the activity of antioxidant system enzymes (AOS) in hepatopancreas of white currant annuals affected by various ectoparasites are given. The research was carried out at the garden and fish farms of the state enterprise «Rybgosp Galitsky» (now Rybgosp «Burshtynskyy» LLC) of the Rogatyn district of the Ivano-Frankivsk region and the farm enterprise «Dobrotvirskiy rybzavod» in the Kamianko-Buzsky district of the Lviv region, located on the warm waters of the Burshtynskaya cooling water reservoirs and Dobrotvirskaya TPS. For the study, white amur of the same age (with body weight 45–47 g) was selected, infected with Dactylogyrus lamellatus, Gyrodactylus ctenopharyngodonis and both parasites at the same time.It was found that white amur of the same age infected with Dactylogyrus lamellatus, Gyrodactylus ctenopharyngodonis and in mixed infestations, the level of TBK-products in hepatopancreas decreased by 25.7% after the treatment with «Granulated brovermectin»; 29.3 and 32.4%, diene conjugates – by 13.5; 21.7 and 18.9% at P < 0.001 in all cases. At the same time using the drugs «Granulated brovermectin» and «Avesstim», the reduction of the above-mentioned indicators in all cases was also reliable (P < 0.001) and was 68.0; 66.3 and 64.0 and 32.0; 35.4 and 36.2%. Concerning the content of lipid hydroperoxides in hepatopancreas of treated fish, its decrease was probable (P < 0,05) only for the combined application of the studied drugs.After use of the above-mentioned preparations the activity of enzymes of antioxidant protection in hepatopancreas of white amur of the same age increased. However, it should be noted that fish affected by dactylosurids, only the activity of superoxide dismutase (SOD) in the complex use of «Granulated brovermectin» and «Avesstim» increased significantly (P < 0.001). In the case of damage by hydroactylics, the activity of superoxide dismutase in hepatopancreas of fish that was administered only «Granulated brovermectin» probably (P < 0,05) increased by 9,4%, and the use of a complex of drugs – by 28,4% (P < 0,001). After mixed infections, as well as for monoinvasia, in the hepatopancreas of white amur of the same age after the use of drugs, an increase in the activity of all the enzymes studied, however, was only significant increase in the activity of SOD (P < 0,05; 0,001). Thus, the complex application of drugs for monogenous doses of fish had a more significant impact on the biological equilibrium in the system LOPs↔AOS.