Zinc Sulfate Heptahydrate Research Articles

Bio-inspired synthesis of ZnO nanoparticles using Taraxacum officinale for antibacterial, antifungal, and anticancer applications

A green synthesis method was developed to fabricate ZnO nanomaterials using Taraxacum officinale (Dandelion) leaf extract as a reducing agent and zinc sulfate heptahydrate as a precursor. Characterization by XRD, EDAX, FTIR, FESEM, and UV–vis spectroscopy confirmed the successful formation of ZnO nanocrystals with enhanced crystallinity following annealing. The ZnO nanomaterials exhibited significant bioactivity, demonstrating 85 % antibacterial efficiency against Escherichia coli and Staphylococcus aureus, along with 70 % antifungal activity. Furthermore, the nanomaterials showed promising anticancer effects, achieving a 60 % reduction in human cancer cell viability. This eco-friendly approach minimizes the use of toxic chemicals typically involved in nanoparticle synthesis, providing a sustainable alternative for producing functional oxide nanoparticles. The use of plant-based reducing agents not only makes the process safer and more biocompatible but also enhances the potential for biomedical applications. ZnO nanomaterials produced through this method offer great promise for antimicrobial therapies and cancer treatment. This efficient, green synthesis method can be further scaled for various applications, highlighting its relevance in fields such as nanomedicine, where bioactivity and eco-friendly synthesis are essential.

Optimisation, Synthesis, and Characterisation of ZnO Nanoparticles Using Leonotis ocymifolia (L. ocymifolia) Leaf Extracts for Antibacterial and Photodegradation Applications

This work presents a green synthesis route, which utilises extracts from an indigenous plant in South Africa, eastern and southern Africa that is understudied and underutilised, for preparing zinc oxide nanoparticles (ZnO NPs). This study involved optimisation of the green synthesis method using Leonotis ocymifolia (L.O.) extracts and performing comparative studies on the effects of using different zinc (Zn) salt precursors; zinc sulphate heptahydrate (Z001) and zinc acetate dihydrate (Z002) to synthesise the ZnO NPs. The comparative studies also compared the L.O-mediated ZnO NPs and chemical-mediated ZnO NPs (Z003). The as-prepared ZnO NPs were tested for their effectiveness in the photodegradation of methylene blue (MB) dye. Furthermore, antibacterial studies were conducted using the agar well diffusion method on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria. The structural, morphological, and optical characteristics of the synthesised ZnO NPs were analysed using XRD, FTIR, SEM, EDS, DRS, and BET techniques. The XRD results indicated that the L.O-mediated ZnO NPs had smaller crystallite sizes (18.24–19.32 nm) than their chemically synthesised counterparts (21.50 nm). FTIR confirmed the presence of biomolecules on the surface of the L.O-mediated NPs, and DRS analysis revealed bandgap energies between 3.07 and 3.18 eV. The EDS results confirmed the chemical composition of the synthesised ZnO NPs, which were made up of Zn and O atoms. Photocatalytic studies demonstrated that the L.O-mediated ZnO NPs (Z001) exhibited a superior degradation efficiency of the MB dye (89.81%) compared to chemically synthesised ZnO NPs (56.13%) under ultraviolet (UV) light for 240 min. Antibacterial tests showed that L.O-mediated ZnO NPs were more effective against S. aureus than E. coli. The enhanced photocatalytic and antibacterial properties of L.O-mediated ZnO NPs highlight their potential for environmental remediation and antimicrobial applications, thus supporting sustainable development goals.

Effect of zinc management on yield, nutrient content, and acquisition of zero-tilled wheat (Triticum aestivum)

A field experiment was conducted during the winter (rabi) season 2018–19 and 2019–20 at Khalsa College, Amritsar, Punjab to find out the effect of soil and foliar application of ZnSO4 .7H2 O on zero till wheat. The experi- ment was conducted in a randomized complete block design with three replications. Treatments were control (noZn), soil application of 12.5, 25, 37.5, 50 kg/ha zinc sulfate heptahydrate (ZnSO4 .7H2 O), and foliar application of 0.5% Zn as one spray at heading initiation (5% ear emergence), one spray at 100% heading (complete ear emergence) and two sprays at heading initiation and 100% heading with a recommended dose of fertilizer. Results showed that the soil application of 50 kg Zn/ha with two foliar sprays of 0.5% zinc sulfate heptahydrate at heading initiation and 100% heading produced the highest yield attributes and grain yield of wheat. Application of 50 kg Zn/ ha with two foliar sprays of 0.5 % Zn at heading initiation and 100% heading registered ~25% higher grain yield over control. Grain Zn content (41.77ppm) percent increase was 33.9 with 50 kg Zn/ha with two foliar sprays of 0.5 % zinc at heading initiation and 100% heading over control (31.20 ppm).Likewise, application of 50 kg Zn/ha with two foliar sprays of 0.5 % Zn at heading initiation and 100% heading recorded the highest net returns among the Zn treatments. Overall, soil application of 50 kg Zn/ha with two foliar sprays of 0.5 % zinc at heading initiation and 100% heading improved the yield, uptake, and profitability of zero-tilled wheat in zinc-deficient soils.

Di-μ-adipato-κ4 O 1,O 1':O 6,O 6'-bis-[(2,2'-di-pyridyl-amine-κ2 N,N')zinc(II)] trihydrate.

The title compound, [Zn2(C6H8O4)2(C10H9N3)2]·3H2O or {Zn2[(C5H4N)2NH]2[μ-(CH2)4(COO)2]2}·3H2O, was separ-ated from the solvothermal reaction of zinc(II) sulfate hepta-hydrate, 2,2'-di-pyridyl-amine and sodium adipate. The dinuclear metal complex has a centrosymmetric structure, with the ZnII atom adopting a highly distorted octa-hedral coordination sphere composed of four oxygen atoms from bridging adipato ligands and two pyridine nitro-gen atoms. In the crystal, the title compound aggregates into a tri-periodic supra-molecular structure through inter-molecular hydrogen-bonding networks of the form O-H⋯O and N-H⋯O.

Nitrogen Requirement and Nitrogen Form Preference by Radermachera hainanensis and R. sinica Plug Seedlings

Abstract Radermachera hainanensis Merr. and R. sinica Hemsl. are popular foliage plants raised from seeds. Nitrogen fertilizer can significantly influence medium pH and EC, plug seedling growth, and photosynthesis. Plug seedlings of both species, each with one pair of leaves, were subjected weekly to nutrient solutions with nitrogen (N) concentrations ranging from 0 to 32 mM. Nitrogen-deficient plants exhibited the lowest leaf area, SPAD-502 value, and shoot dry weight but the highest root-to-shoot ratio. Leaf area, SPAD-502 value, and root and shoot dry weights increased with increasing N concentration up to 12-16 mM, and gradually decreased thereafter. Well-grown plants with 12-16 mM N exhibited peak maximal fluorescence (Fm), photochemical quenching (qP) and the lowest minimal fluorescence (Fo) and non-photochemical quenching (qN). Seedlings received nutrient solutions with varying proportions of NH4-N: NO3-N (0:100, 25:75, 50:50, 75:25, and 100:0, in percentages) at 16 mM N. Both species exhibited reduced leaf area, SPAD-502 value, and plant dry weights when supplied with only NO3-N or NH4-N. Maximum growth was achieved at a 50% NH4-N: 50% NO3-N ratio. Species used in this study: Golden jasmine tree, Radermachera hainanensis Merr.; China Doll, Radermachera sinica Hemsl. Chemicals used in this study: ammonium nitrate; sodium dihydrogen phosphate; potassium sulphate; potassium nitrate; magnesium sulfate heptahydrate; calcium sulfate dihydrate; calcium nitrate tetrahydrate; ammonium sulfate; boric acid anhydrous; potassium chloride; ferrous sulfate heptahydrate; disodium ethylenediaminetetraacetic acid dihydrate; copper sulfate pentahydrate; zinc sulfate heptahydrate; molybdic acid; manganese sulfate monohydrate; nickel sulfate hexahydrate; sodium hydroxide.

Cellulose Nanofiber-Based Nanocomposite Films with Efficient Electromagnetic Interference Shielding and Fire-Resistant Performance.

Cellulose nanofiber (CNF) has been widely used as a flexible and lightweight polymer matrix for electromagnetic shielding and thermally conductive composite films because of its excellent mechanical strength, environmental performance, and low cost. However, the lack of flame retardancy seriously hinders its further application. Herein, renewable and biomass-sourced l-arginine (AR) was used to surface-modify ammonium polyphosphate (APP) and an environmentally friendly biobased flame retardant was synthesized by the coordination of zinc sulfate heptahydrate (ZnSO4·7H2O), which was named AAZ. AAZ was deposited on the surface of CNF by electrostatic adsorption and Zn2+ complexation. The biobased compatibilizer Triton X-100 was employed to assist the exfoliation of graphene nanoplatelets (GNPs) and their dispersion in the CNF matrix. Due to the formation of a dense lamellar layer resembling a shell structure, the CNF/GNPs composite films with a tensile strength of 52 MPa were obtained via vacuum-assisted filtration. Because the phosphorus-containing group produces a protective layer of PxOy compound and promotes the formation of a carbon layer by CNF and the combustion releases ammonia gas, the fire-resistant performance of the composite films was greatly improved. Compared with the pure CNF film, the composite film exhibits 33% reduction in PHRR value and 40% reduction in THR. In addition, the CNF/GNPs composite film with 20 wt % GNPs possessed high conductivity (2079.2 S/m) and electromagnetic interference (EMI) shielding effectiveness (37 dB). The ultrathin CNF/GNPs composite films have excellent potential for use as efficient flame retardant and EMI shielding materials.

Effect of pH on Toxicity of Zinc Sulphate Hydrate Solutions Using the Spirotox Test

Introduction: Spirostomum ambiguum is commonly used as a biological indicator due to its high sensitivity to heavy metals. It functions normally across a wide pH range of 4.5 to 8.0. Testing on protozoan organisms provides a general understanding of the impact of pH on the toxicity of zinc in pharmaceuticals. By investigating the toxicity of zinc in environment with varying pH levels, the optimal environment for zinc absorption can be determined. Objective: The objective of this study is to assess the toxicity of zinc sulphate solutions using different forms of hydrating and at various pH levels, applying the Spirotox method. Methods: An experiment was conducted using a solution of pharmaceutical substances, specifically zinc sulphate heptahydrate and zinc sulphate monohydrate, with a concentration of 0.01 M. The study aimed to investigate the effect of the solution on the lifespan of S.ambiguum within the pH range of 4.8-6.5. Results: Arrhenius activation energy (Ea linear correlates with LD50 oral toxicity for rats) decreases as pH increases from 4.8 to 6.5 for ⋅7 and ⋅ solutions. The values decrease from 140±10 kJ/mol to 55±10 kJ/mol and from 110±2 kJ/mol to 60±10 kJ/mol, respectively. Conclusion: It was found that the toxicity of zinc sulphate hydrate solution towards S. ambiguum increased as the pH value increased. Hydrating of the active pharmaceutical substance was a crucial factor, it is critically important for preparation of solutions of an active pharmaceutical substance. Keywords: zinc sulphate, Spirotox test, pH, activation energy, toxicity.

Colchicine-capped ZnO nanoparticles: Elucidation of their cytotoxicity potential against MDA-MB 231 cancer cells

This research descibes a pioneering approach aimed at preparing zinc oxide nanoparticles (ZnO-NPs) with colchicine as the reducing and capping agent. Colchicine-loaded ZnO-NPs (CHZnO-NPs) were prepared by adding colchicine to the zinc sulfate heptahydrate solution. The CHZnO-NPs formulation was then characterized to determine the morphology, size, crystallinity, elemental composition and vibrational properties. Upon characterization, CHZnO-NPs were studied for their cytotoxic effect against the breast cancer cell line (MDA-MB-231). The successful biosynthesis of CHZnO-NPs was initially confirmed visually by the changes in the mixture color, from light-yellow to white cloudy. The best CHZnO-NPs formulation selected was F3, which possessed 10 mg/mL of colchicine. Formulation (F3) had the smallest mean particle diameter of 43.77 nm and the lowest zeta potential of −19.60 mV. It also had 92.21 ± 0.012 % encapsulation efficiency and 20.86 ± 0.005 % drug loading. Formulation (F3) displayed a hexagonal wurtzite structure with irregular morphology. The observation of colchicine peaks on the FTIR spectra of F3 proved the role of colchicine as a reducing and capping agent during the synthesis of ZnO-NPs. Besides, the in-vitro cell cytotoxicity study on the MDA-MB-231 cell line revealed a significant reduction in cell proliferation at the concentration of 25 μg of colchicine and F3. Further, studies on the cellular migration potential also demonstrated concentration-dependent activity. Overall, CHZnO-NPs were shown to be successfully synthesized via an environmental-friendly procedure and colchicine acted as a capping agent to regulate the particle size, and aggregation, in addition to its anticancer properties.

Multivariate mesoporous MOFs with regulatable hydrophilic/hydrophobic surfaces as a versatile platform for enzyme immobilization

Zeolitic imidazole frameworks materials-8 (ZIF-8), a member of the metal-organic framework (MOFs) series, have been extensively used as a host matrix for enzyme immobilization. However, the microporous structure and hydrophobicity, as well as the protonation of the precursor 2-methylimidazole (2-MeIm) of ZIF-8, remain considerable challenges in maintaining the activity of immobilized enzymes. Here, novel multivariate mesoporous MOFs (mMOFs) with regulatable hydrophilic/hydrophobic surfaces were designed by the multivariate competitive strategy and pore modification engineering. 3-Methyl-1H-1,2,4-triazole (3-MTZ) and 5-methyltetrazole (5-MTA) were employed to partially replace 2-MeIm. These were then combined with zinc sulfate heptahydrate (soft templates) to yield mMOFs in a methanol solution. As a proof-of-concept application, we used mMOFs as carriers for enzyme immobilization and investigated the properties of the immobilized enzymes. Benefiting from their mesoporous structure, hydrophilic surface, and improved microenvironment, multivariate mMOFs exhibit a strong ability to stabilize enzyme conformation and increase enzyme activity compared with traditional ZIF-8. Our study offers an avenue for the controllable preparation of well-designed MOF structures, which will further broaden the application opportunities of MOF materials for enzyme immobilization.

Interactions of Microbial Inoculations with Fertilization Options and Crop Establishment Methods on Modulation of Soil Microbial Properties and Productivity of Rice

A field investigation was undertaken to assess the interactive effects of different crop establishment methods (CEMs) and microbial formulations in field conditions. The two year experiment was carried out in kharif season (June to October) of both 2013–14 and 2014–15 at Research Farm of ICAR-Indian Agricultural Research Institute, New Delhi India. The CEMs studied consist of puddled transplanted rice (PTR), system of rice intensification (SRI) and aerobic rice system (ARS); while microbial formulations consist of Anabaena sp. (CR1)+Providencia sp. (PR3) consortium (MC1) and Anabaena–Pseudomonas biofilm formulation (MC2). The microbial inoculation was applied with 75% recommended dose of nutrients (RDN) (90 kg nitrogen ha-1 and 19.35 kg phosphorus ha-1) and compared with 100% RDN. Soil microbiological properties showed significantly higher values in SRI in the first year; while in the second year, SRI and PTR remained on par and superior to ARS. Among the combinations of nutrient supplements, application of Anabaena–Pseudomonas biofilm formulation (MC2) with 75% RDN was superior in terms of all microbiological attributes studied [acetylene reduction activity (ARA), soil chlorophyll, soil dehydrogenase activity, microbial biomass carbon and alkaline phosphatase activity]. Application of microbial inoculation led to an increase in milled rice yield from 230 to 240 kg ha-1. Among the different combinations of CEMs and inputs investigated the application of 75% RDN+MC2+zinc (Zn) (soil applied 5 kg Zn ha-1 through zinc sulphate heptahydrate) in SRI can be a judicious option to maximize rice yield and soil health.

Synthesis and Characterization of Zn(II) Complex of 4-chloro-2-(((2-phenoxyphenyl)imino)methyl)phenol and its Biological Efficacies: DNA Interaction, ADMET, DFT and Molecular Docking Study.

Condensing 2-phenoxyaniline with 5-chlorosalicyldehyde under reflux conditions, a 4-chloro-2-(((2-phenoxyphenyl)imino)methyl)phenol Schiff base has been Synthesized. A zinc complex was synthesized by combining the ligand in a 1:1 molar ratio with zinc sulphateheptahydrate. Mass spectroscopy, NMR, infrared, and elemental analysis were used to characterize the ligand and zinc complex. By measuring the molar conductance, the non-electrolytic character of the complex was confirmed. The zinc ion is coordinated in a pentadentate manner, according to an IR and NMR investigation. Viscosity measurements, absorption and fluorescence spectroscopy were utilized to examine the complex's interaction with CT (calf thymus) DNA. Furthermore, the ligand and complex's ADMET characteristics were ascertained through the use of ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) study. Calculation of the different electronic parameters of the optimized structure through Density Functional Theory (DFT) indicated the stability of the Zn(II) complex. Molecular docking study reflected the future opportunity for the consideration of Zn(II) complex to fight against Alzheimer and Glaucoma diseases.

Impact of foliar application of iron and zinc fertilizers on grain iron, zinc, and protein contents in bread wheat (Triticum aestivum L.).

Micronutrient deficiencies, particularly iron (Fe) and zinc (Zn), are prevalent in a large part of the human population across the world, especially in children below 5 years of age and pregnant women in developing countries. Since wheat constitutes a significant proportion of the human diet, improving grain Fe and Zn content in wheat has become important in improving human health. This study aimed to quantify the effect of foliar application of iron sulfate heptahydrate (FeSO4.7H2O) and zinc sulfate heptahydrate (ZnSO4.7H2O) and their combination on grain Fe and Zn concentrations, as well as grain protein content (GPC). The study also aimed to assess the utility of these applications in large field conditions. To address this issue, field experiments were conducted using 10 wheat cultivars and applying a foliar spray of FeSO4.7H2O (0.25%) and ZnSO4.7H2O (0.50%) separately (@400 L of solution in water per hectare during each spray) and in combination at two different crop growth stages (flowering and milking) for three consecutive crop seasons (2017-2020). The study used a split-plot design with two replications to assess the impact of foliar application on GFeC, GZnC, and GPC. In addition, an experiment was also conducted to assess the effect of soil (basal) @ 25 kg/ha ZnSO4, foliar @ 2 kg/ha, ZnSO4.7H2O (0.50%), and the combination of basal + foliar application of ZnSO4 on the grain micronutrient content of wheat cultivar WB 02 under large field conditions. GFeC increased by 5.1, 6.1, and 5.9% with foliar applications of FeSO4, ZnSO4, and their combination, respectively. GZnC increased by 5.2, 39.6, and 43.8% with foliar applications of FeSO4, ZnSO4, and their combination, respectively. DBW 173 recorded the highest increase in GZnC at 56.9% with the combined foliar application of FeSO4 and ZnSO4, followed closely by HPBW 01 at 53.0% with the ZnSO4 foliar application, compared to the control. The GPC increased by 6.8, 4.9, and 3.3% with foliar applications of FeSO4, ZnSO4, and their combination, respectively. Large-plot experiments also exhibited a significant positive effect of ZnSO4 not only on grain Zn (40.3%, p ≤ 0.001) and protein content (p ≤ 0.05) but also on grain yield (p ≤ 0.05) and hectoliter weight (p ≤ 0.01), indicating the suitability of the technology in large field conditions. Cultivars exhibited a slight increase in GFeC with solitary foliar applications of FeSO4, ZnSO4, and their combination. In contrast, a significant increase in GZnC was observed with the foliar application of ZnSO4 and the combined application of FeSO4 and ZnSO4. In terms of GPC, the most significant enhancement occurred with the foliar application of FeSO4, followed by ZnSO4 and their combination. Data demonstrated the significant effect of foliar application of ZnSO4 on enhancing GZnC by 39.6%. Large plot experiments also exhibited an increase of 40.3% in GZnC through the foliar application of ZnSO4, indicating the effectiveness of the technology to be adopted in the farmer's field.

Agronomic performance and zinc concentration in plant tissues of several rice fields varieties with zinc sulfate heptahydrate (ZnSO4) fertilization

Agronomic performance and zinc concentration in plant tissues of several rice fields varieties with zinc sulfate heptahydrate (ZnSO₄) fertilization

Green synthesis of ZnO nano-crystals using Chenopodium album L. Leaf extract, their characterizations and antibacterial activities

We present an effective, simple and eco-friendly method for the green synthesis of ZnO nano-crystals. An aqueous extract of Chenopodium album leaf was used as a biological reducing agent for the fabrication of ZnO nano-crystals from the zinc sulphate heptahydrate. The as-prepared and annealed powder samples were characterized using advanced techniques. The results confirmed the presence of ZnO crystals with a broad size distribution, whose crystallinity was noticed to improve substantially after the annealing. Further, in antibacterial activity test of ZnO nano-crystals performed on Escherichia coli and Staphylococcus aureus microorganisms, inhibitory effect was found to increase with concentration. Compared to Gram-positive bacteria, ZnO nano-crystals showed more resistance to Gram-negative bacteria. This green synthesis route of ZnO nano-crystals is novel and aims to reduce the use of toxic chemicals in fabricating the nanoparticles of functional oxides, which have a wide range of potential applications in general and biomedical science, in particular.

Changes in serum and faecal zinc concentrations following supplementation with zinc sulphate heptahydrate of extensively grazed dairy cattle in New Zealand

ABSTRACTAimsTo examine the relationship in dairy cattle between serum and faecal Zn concentrations and daily intake of Zn supplemented with an oral drench; and whether total daily intake (TDI) of Zn in dairy cattle can be predicted from single measurements of Zn concentration in serum or faeces.MethodsA convenience sample of 20 animals from three stock classes (lactating cows, dry cows, heifers), that had not received Zn supplementation in the previous 60 days, was enrolled in the study. From Days −7 to −1, animals received no Zn supplementation. On Day 0, 15 animals per class were assigned daily drenching with increasing doses of ZnSO4.7H2O while five remained controls. From Days 0–6, treatment animals received 12.5 mg/kg LWT of Zn/day; from Days 7–13, 25 mg/kg LWT Zn/day and from Days 14–20, 37.5 mg/kg LWT Zn/day. Animals co-grazed within each stock class. Pasture, serum and faecal samples were collected at the start and at weekly intervals before each increase in Zn supplementation. Mixed and non-parametric models were used to assess treatment effects and whether daily intake of Zn could be predicted from Zn concentrations in serum and faeces.ResultsDosing with 0, 12.5, 25.0 and 37.5 mg Zn/kg LWT resulted in serum Zn concentrations of 12.1, 16.7, 27.2 and 35.8 µmol/L in heifers, 13.3, 17.1, 26.4 and 40.0 µmol/L in dry and 11.9, 12.1, 23.4 and 27.2 µmol/L in lactating cows. Dosing with the same amounts of Zn resulted in faecal Zn concentrations of 2.95, 21.72, 40.32 and 53.27 mmol/kg DM in heifers, 2.81, 23.77, 55.16 and 68.20 mmol/kg DM in dry and 3.00, 12.71, 34.86 and 57.53 mmol/kg DM in lactating cows, respectively. Treatment elevated serum and faecal Zn concentrations above controls (p < 0.001). Supplemented lactating cows had lower serum Zn concentrations than dry cows or heifers (p < 0.01). Supplemented dry cows had faecal DM Zn concentrations higher than heifers or lactating cows (p < 0.05). Analysis showed serum and faecal Zn concentrations could predict TDI of Zn (p < 0.001). Concentrations of Zn in faeces estimated TDI of Zn within a narrower predictive interval than serum Zn concentrations.Conclusions and clinical relevanceConcentrations of Zn in serum and faeces were positively associated with TDI of Zn in dairy cattle and could predict TDI of Zn. When using serum and faecal Zn concentrations to estimate TDI Zn, stock class must be accounted for.

Fabrication, Characterization and In Vitro Cell Cytotoxicity of ZnO Nanoparticles on MDA‐MB 231 Breast Cancer Cell Line

AbstractThe intention of this study was to prepare zinc oxide nanoparticles (ZnO‐NPs) via bottom‐up approach and evaluate their physiochemical, antimicrobial and anticancer properties. ZnO‐NPs were first prepared by the direct precipitation method with zinc sulphate heptahydrate, followed by characterization tests using sophisticated analytical and imaging instruments. Upon characterization, ZnO‐NPs were studied for their antimicrobial activities against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa as well as their anticancer activity against breast cancer cell line (MDA‐MB 231). The ZnO‐NPs possessed a mean particle diameter of 225.2 nm and a zeta potential of −15.8 mV. A sharp absorption peak of ZnO was recorded at 354 nm via UV‐Visible spectroscopy. Hexagonal wurtzite shape of ZnO‐NPs has been observed and confirmed by Scanning Electron Microscopy (SEM) and X‐Ray diffraction (XRD). ZnO‐NPs exhibited excellent antimicrobial activity against the Gram‐positive bacteria, Staphylococcus aureus. In an in vitro cell cytotoxicity study, ZnO‐NPs showed a dose‐dependent relationship against MDA‐MB 231 breast cancer cells. It was observed that as the concentration of ZnO‐NPs increased (0.25 μg to 35 μg), there was a sharp reduction in the proliferation rate of the cancer cell line. Overall, our study reported successful synthesis of ZnO‐NPs, which can be efficient antibacterial and anticancer agents.

The Effect of Foliar Zinc Application on the Leaf Chlorophyll Concentrations and Grain Yields of the Winter Wheat (Triticum aestivum L.) in the Field Experiments of Two Seasons

This research examined the effectiveness of zinc foliar fertilizers. Two winter wheat varieties, GK Pilis and Zvezdana, were tested in a Zn-limited meadow chernozem soil. Crop yields and chlorophyll concentrations of the leaves were examined in the growing seasons of 2020/2021 of favorable weather conditions and 2021/2022 of drought weather conditions. In the experiment, three treatments were carried out with monozinc foliar fertilizer of a concentrated 12% (w/v) zinc sulfate heptahydrate solution, and the fourth plot was designed as the untreated control. The first monozinc treatment was applied at a dose of 1 L ha−1 and the second monozinc treatment at a dose of 2 L ha−1. These treatments were applied at tillering (BBCH 21–22). The third monozinc treatment was applied at a dose of 1 L ha−1 at tillering and flag leaf emergence (BBCH 21–22, BBCH 37). Samples were taken several times in the vegetation period, and measurements were made using different methods (SPAD, determination of total chlorophyll concentration from acetone solution) before and after Zn treatments. In 2020/2021 of favorable weather conditions, the yield of Zvezdana was 5.47 t ha−1 and that of Gk Pilis was 6.11 t ha−1, compared to 2021/2022 of drought weather conditions, in which year the yield of Zvezdana was 3.14 t ha−1 and that of Gk Pilis was 2.79 t ha−1. Yields of both varieties increased with increasing NPK nutrient doses. In 2021/2022, even at nutrient level D (NPK basal fertilizer and two-times N topdressing), we harvested on average twice as much as in 2020/2021 at nutrient level A (N basal fertilizer and once N topdressing). No significant effects were found between the foliar fertilizers in 2020/2021. In 2021/2022, Zn treatments, mainly at four nutrient levels tended to increase the yields of both varieties. The first treatment resulted in a grain yield reduction of 3% for GK Pilis, and the second treatment resulted in a grain yield reduction of 1.2% for Zvezdana. After the second and third treatments, grain yield reductions were 0.1%–0.5%. Although there was no significant difference in terms of the main averages, foliar fertilizer was found to be effective in the second year. The relationship between chlorophyll and grain yield was found to depend on the year and variety. A weak correlation (r = 0.33, p &lt; 0.05) was found between the total chlorophyll concentration in leaves for Zvezdana in 2021, and a medium correlation for GK Pilis (r = 0.67, p &lt; 0.05). Concerning grain yield and chlorophyll concentration, a strong correlation (r = 0.81, p &lt; 0.05) was found for Zvezdana in 2022, and a weak correlation (r = 0.44, p &lt; 0.05) for GK Pilis. A strong correlation was found between the total leaf chlorophyll concentration and SPAD index in both years (r = 0.81, p &lt; 0.05)

The Structural Changes and Its Impact on Optical Dispersion of PVA/PVP Embedded with Zinc Sulphate Nanoparticles and Irradiated with Electron-Beam

PVA/PVP films doped with zinc sulphate heptahydrate (ZnSO4·7H2O) nanoparticles (NPs) are prepared using an in situ chemical method. Furthermore, as-prepared samples are irradiated with an electron beam at doses of 20, 25, 30, 35, and 40 kGy. Elemental analyses using energy dispersive X-ray spectroscopy (EDXS) of the fresh films showed the existence of both zinc oxide and zinc sulphide as main and minor phases in sequence. Moreover, scanning electron microscope measurements (SEM) revealed that embedded Zn NPs have regular and evenly distributed pores inside PVA/PVP network. On the other hand, the structural variations due to e-beam irradiation are monitored by a high-resolution-transmission electron microscope (HR-TEM), which displayed that encapsulated Zn NPs are well dispersed and capped inside PVA/PVP structural network. The measured optical absorption coefficient (α) of PVA/PVP/zinc sulphate nanocomposite films showed an increase against e-beam dose. Moreover, the calculated optical energy gap, Eg, exhibited a reduction from 3.31 to 2.82 eV against the increase in irradiation dose. Evaluated values of wavelength at zero material dispersion (WZMD) indicated that e-beam irradiation can tune the structure of the studied samples to be used as a data transmitter in optical fiber telecommunication in the IR spectral range of 1.6842–1.0351 μm.

Interactive effects of temperature and salinity on toxicity of zinc oxide nanoparticles towards the marine mussel Xenostrobus securis

Growing application of zinc oxide nanoparticles (ZnO-NPs) in global market has led to the concern over their potential environmental impacts. Filter feeders like mussels are prone to nanoparticles due to their superior filter-feeding ability. Temperature and salinity of coastal and estuarine seawaters often vary seasonally and spatially, and their changes may jointly influence physicochemical properties of ZnO-NPs and thus their toxicity. This study, therefore, aimed to investigate the interactive effect of temperatures (15, 25 and 30 °C) and salinities (12 and 32 PSU) on physicochemical properties and sublethal toxicity of ZnO-NPs towards a marine mussel Xenostrobus securis, and to compare that with the toxicity caused by Zn2+ ions (zinc sulphate heptahydrate). The results revealed increased particle agglomeration but decreased zinc ion release of ZnO-NPs at the highest temperature and salinity condition (30 °C and 32 PSU). After exposure, ZnO-NPs significantly reduced survival, byssal attachment rate and filtration rate of the mussels at high temperature and salinity (30 °C and 32 PSU). Glutathione S-transferase and superoxide dismutase activities in the mussels were suppressed at 30 °C. These aligned with the augmented zinc accumulation with increasing temperature and salinity which could likely be attributable to increased particle agglomeration of ZnO-NP and enhanced intrinsic filtration rate of the mussels under these conditions. Together with the observed lower toxic potency of Zn2+ compared to ZnO-NPs, our results suggested that the mussels might accumulate more zinc through particle filtration under higher temperature and salinity, eventually resulting in elevated toxicity of ZnO-NPs. Overall, this study demonstrated the necessity to consider the interactive effect of environmental factors such as temperature and salinity during the toxicity assessment of nanoparticles.

Study on the involvement of Zinc in homeostasis under intestinal Inflammation

Background &amp; Aim: Zinc is one of the essential trace elements, and adequate amounts of zinc are crucial for good health. It is thought that zinc is absorbed primarily in the small intestine and also plays an important role in maintaining intestinal homeostasis. Small intestine is a key organ involved in digestion/absorption and is also sensitive to intestinal bacteria and easily inflamed. Alkaline phosphatase (ALP), which has zinc as its active center, detoxifies lipopolysaccharide and is thought to be involved in the suppression of small intestinal inflammation. In this study, we investigated the role of zinc in small intestinal inflammation using a mouse model, as well as the transporters ZIP4 and ZIP14, which are involved in ALP and zinc absorption. Materials &amp; Methods: Male ICR mice were used. Indomethacin (IM, 12 mg/kg s.c.) was administered to mice to induce small intestinal inflammation. Four days prior to IM administration, zinc sulfate heptahydrate (Zn) was administered twice daily for 6 days. Forty-eight hours after IM administration, the animals were sacrificed and the lesion area was measured. Hematoxylin and eosin staining was performed. Myeloperoxidase (MPO) activity in the ileum was measured as an indicator of the degree of inflammation. ZIP4 and ZIP14 mRNA expression levels were examined by RT-PCR. ALP activity of the ileal was examined. IEC-6 cells, a rat small intestinal epithelial cell line, were seeded in 96-well plates, and Zn was applied 24 hours after seeding, and IM was added 24 hours after seeding. Cell viability was measured 24 hours after the addition of IM. Results: After administration of IM, bloody stools were observed from 24 hours after administration. At 48 hours after IM administration, disruption of villi structure and shortening of small intestine length were observed in the jejunum and ileum, and MPO activity was significantly increased. ZIP4 and ZIP14 mRNA expression levels were decreased in the small intestine 48 hours after IM treatment, and ALP activity was increased compared to the Vehicle group. On the other hand, pretreatment with Zn suppressed the occurrence of small intestinal inflammation, and the disruption of small intestinal villi structure was weak. The increase in MPO activity induced by IM tended to be suppressed by Zn pre-administration. ZIP4 and ZIP14 mRNA expression in Zn pre-administration group was same as in the IM alone group, and ALP activity was higher than in the IM alone group. IM treatment of IEC-6 also decreased cell viability in a concentration-dependent manner, which was inhibited by pretreatment with Zn in a concentration-dependent manner. Conclusion: These results suggest that zinc is effective in preventing the occurrence of NSAID-induced small intestinal inflammation. ZIP4 and ZIP14 are normally highly expressed in the intestinal epithelium and contribute greatly to zinc absorption, but the amount of zinc absorption may be decreased during inflammation induc University This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.