Zinc Nutrition Research Articles

Metabolic adaptations of Escherichia coli to extended zinc exposure: insights into tricarboxylic acid cycle and trehalose synthesis

Balanced bacterial metabolism is essential for cell homeostasis and growth and can be impacted by various stress factors. In particular, bacteria exposed to metals, including the nanoparticle form, can significantly alter their metabolic processes. It is known that the extensive and intensive use of food and feed supplements, including zinc, in human and animal nutrition alters the intestinal microbiota and this may negatively impact the health of the host. This study examines the effects of zinc (zinc oxide and zinc oxide nanoparticles) on key metabolic pathways of Escherichia coli. Transcriptomic and proteomic analyses along with quantification of intermediates of tricarboxylic acid (TCA) were employed to monitor and study the bacterial responses. Multi-omics analysis revealed that extended zinc exposure induced mainly oxidative stress and elevated expression/production of enzymes of carbohydrate metabolism, especially enzymes for synthesis of trehalose. After the zinc withdrawal, E. coli metabolism returned to a baseline state. These findings shed light on the alteration of TCA and on importance of trehalose synthesis in metal-induced stress and its broader implications for bacterial metabolism and defense and consequently for the balance and health of the human and animal microbiome.

Contribution of plant growth-promoting endophytic bacteria from hyperaccumulator to non-host plant zinc nutrition and health

Application of microbial agents is a novel strategy to improve the quality and health of plant, which can be used to increase zinc (Zn) uptake and alleviate Zn toxicity. Here, endophytic bacteria with Zn solubilizing and growth-promoting properties were isolated from hyperaccumulating ecotype (HE) of Sedum alfredii Hance and their effects on Zn absorption and accumulation of non-hyperaccumulating ecotype (NHE) were studied. The results showed that most endophytic bacteria of HE have good Zn solubilizing or growth-promoting properties. Under the condition of 20 μM ZnSO4, the biomass of NHE inoculated with SaPS1, SaEN2, SaPR2, SaBA2, SaBA3 was 2.8–3.2 times higher than that of non-inoculation control, and the Zn concentration of shoots was increased by 45.9, 89.0, 53.7, 77.5, and 42.6% after inoculation with SaPA1, SaP1, SaEN2, SaBA1, and SaBA2. Under the condition of 100 μM ZnSO4, inoculation with SaVA1, SaPS3, SaB1, SaPR1, and SaEN3 alleviated Zn stress and significantly reduced Zn concentration of shoots. Therefore, endophytic bacteria can be an effective means of improving plant Zn nutrition quality in the normal condition and benefit plant health in the stress environment.

Effect of Dietary Zinc on the Antioxidant Parameters of Juvenile Common Carp (Cyprinus carpio)

Zinc (Zn) is an essential micro mineral needed for the proper growth and immune function of fish. This investigation was designed to examine the antioxidant role of a fortified diet with different Zn levels in the muscle and liver tissues of carp fry. A four-iso-nitrogen (35% crude protein) practical diet was produced that included graded levels of dietary zinc sulphate as a nutritional zinc resource in the fundamental diet supplemented with increased zinc levels (T1, control, 85 mg Zn kg-1, T2 105 mg Zn kg-1, T3, 125 mg Zn kg-1 and T4, 145 mg Zn kg-1). Even though the SOD and CAT analysis results did not show a linear increase in the increasing Zn ratio in the diets, higher values were obtained compared to the control groups. SOD highest values in T3 for the liver (0.713 ± 0.220 U/ml) and T1 for muscle (0.751 ± 0.144 U/ml), CAT values were highest in T2 for the liver (0.849 ± 0.115 nmol/dk/m) and T2 for muscle (1.059 ± 0.148 nmol/dk/m) was obtained. MDA values were completely higher for the muscle than for the control group, and for the liver, a lower value was obtained in the T2 trial group than in the control group (1.671 ± 0.230 µM). The results of the study showed that Zn contributed significantly to the nutrition of carp fish. It can be concluded that the findings of SOD and CAT analysis endorse the positive contributions of using 105 mg Zn in the diets to promote the antioxidant defense of juvenile carp fish.

Agronomic Effectiveness of Zinc Sources as Micronutrient Fertilizers: A Comprehensive Review’

Zinc naturally occurs in the earth's crust as part of rocks and ore minerals. The average concentration of zinc in the lithosphere is 80 mg kg-1. While zinc is relatively immobile in soil, it is moderately mobile in plants. As a vital micronutrient for both humans and animals, zinc plays an essential role in plants as a catalytic, structural and regulatory cofactor for many enzyme reactions. It is necessary for the metabolism of carbohydrates, protein synthesis, the biosynthesis of growth hormones (particularly indoleacetic acid) and the maintenance of cell membrane integrity. Zinc deficiency is a widespread problem affecting both plants and humans in many regions of the world. To address this issue and improve plant zinc nutrition and yields, both soil and foliar zinc fertilizers have been applied. The agronomic effectiveness of Zn fertilizers has been related to the management factors such as placement, source type, seed treatment, foliar spray and biofortification that can affect the effectiveness of Zn fertilizers.

Zinc Protects against Swine Barn Dust-Induced Cilia Slowing.

Agricultural workers exposed to organic dust from swine concentrated animal feeding operations (CAFOs) have increased chances of contracting chronic lung disease. Mucociliary clearance represents a first line of defense against inhaled dusts, but organic dust extracts (ODEs) from swine barns cause cilia slowing, leading to decreased bacterial clearance and increased lung inflammation. Because nutritional zinc deficiency is associated with chronic lung disease, we examined the role of zinc supplementation in ODE-mediated cilia slowing. Ciliated mouse tracheal epithelial cells were pretreated with 0-10 µg/mL ZinProTM for 1 h, followed by treatment with 5% ODE for 24 h. Cilia beat frequency (CBF) and protein kinase C epsilon (PKCε) activity were assayed. ODE treatment resulted in cilia slowing after 24 h, which was reversed with 0.5 and 1.0 µg/mL ZinPro pre-treatment. No zinc protection was observed at 50 ng/mL, and ciliated cells detached at high concentrations (100 µg/mL). ZinPro alone produced no changes in the baseline CBF and showed no toxicity to the cells at concentrations of up to 10 µg/mL. Pre-treatment with ZinPro inhibited ODE-stimulated PKCε activation in a dose-dependent manner. Based on ZinPro's superior cell permeability compared to zinc salts, it may be therapeutically more effective at reversing ODE-mediated cilia slowing through a PKCε pathway. These data demonstrate that zinc supplementation may support the mucociliary transport apparatus in the protection of CAFO workers against dust-mediated chronic lung disease.

Enhancing rice yields through foliar application of essential micronutrients: A study on zinc, copper, and boron nutrition in context of Nepal

Quantitative data on the effects of essential micronutrients—boron, zinc, and copper—on rice (Oryza sativa L.) are limited, which hampers optimized crop management strategies. This study aimed to enhance rice yields through the foliar application of these micronutrients. Conducted in 2022 at Gokuleshwor Agriculture and Animal Science College using a randomized complete block design with three replications, the research tested different combinations of these elements on the local rice variety Kaljade. The treatments included: T1 (control), T2 (zinc), T3 (copper), T4 (boron), T5 (Zn + Cu + B), T6 (Zn + Cu), and T7 (Zn + B), with doses of 5 kg/ha for zinc and 2 kg/ha each for boron and copper. The results demonstrated significant effects of the micronutrient treatments on all parameters studied. Notably, the combination of zinc, copper, and boron (Zn + Cu + B) led to the most favorable outcomes. This treatment resulted in the shortest maturity duration (116.3 days after transplanting), the highest number of tillers (22.5), and the tallest plant height (107.7 cm). Additionally, Zn + Cu + B produced the greatest leaf area index (0.0724), the highest number of grains per panicle (22.6), and the heaviest thousand-grain weight (21.83 g). Furthermore, Zn + Cu + B achieved the highest biological yield (12.35 t/ha) and grain yield (5.9 t/ha), markedly higher than the control treatment (4.12 t/ha). These findings highlight the significant role of zinc, copper, and boron in rice cultivation. The study underscores the potential of foliar application techniques to optimize micronutrient availability, thereby enhancing rice yields. For future agricultural practices in the study area, focusing on zinc, copper, and boron nutrition is crucial to further augment crop productivity and ensure food security.

Optimizing Grain Zinc Biofortification in Bread Wheat: Innovative Fertilization Strategies for Field Conditions

Zinc deficiency in humans is of worldwide concern. The aim of this study is to enrich grains with Zn through soil application at different doses and foliar application from various sources. For this purpose, two independent field experiments have been conducted. In Experiment I, Zn (ZnSO4.7H2O) was applied through soil as basal and top dressing. The treatments were Zn0: control, Zn1: 5 kg Zn ha−1 as basal, Zn2: 2.5 kg Zn ha−1 as basal and 2.5 kg Zn ha−1 as top dressing and Zn3: 5 kg Zn ha−1 as top dressing. In Experiment II, Zn was applied as foliar from different sources (ZnSO4.7H2O, Zn-Methionine, Zn-Polyol and Zn-EDTA) at different growth stages with or without urea. In both experiments, Zn concentration in the grain and leaves of wheat and grain yield increased while phytic acid and PA/Zn molar ratio decreased due to soil or foliar Zn application. It was determined that the application of ZnSO4.7H2O in the soil at the top dressing period in Experiment I and the application of ZnSO4.7H2O and Zn-Methionine forms together with urea in Experiment II were effective methods in increasing grain yield and grain Zn concentration. The application of ZnSO4.7H2O from both soil and foliar sources, especially in combination with urea, has proven to be highly effective in the Zn biofortification of wheat grains. It is possible to assert that the use of ZnSO4.7H2O, which is cost-effective compared to other sources, could contribute significantly to enhancing zinc nutrition in humans.

Zinc nutrition at first feeding imprints a programming effect on growth and hepatic lipid metabolism in juvenile rainbow trout

The objective of this study was to determine whether supplementation with deficient zinc (Zn) or/and excess copper (Cu) in the first-feeding diet of rainbow trout fry influenced the growth and physiological regulation of juvenile fish subjected to similar challenge feeds again. The feeding trial lasted for 24 weeks and had three phases. In phase I, rainbow trout fry was treated with one of four different plant-based feeds containing or exempting Zn or Cu supplementation (coded Zn-Cu-, Zn+Cu-, Zn-Cu+, Zn+Cu+) for 6 weeks. Thereafter, all groups were fed a common commercial feed for the next 12 weeks (phase II). In phase III, all groups were fed with the “challenge diet” for another 6 weeks which was the basal diet (Zn-Cu-) except that the vegetable oils were replaced by fish oil. The results demonstrated that fish fed a Zn+ diet at first feeding increased larval growth (phase-I), but it had no effect on growth at the end of phase II when fed commercial feeds. In phase III, when re-introduced to a Zn- challenge diet, the feed intake and growth of juveniles with Zn+ history significantly increased, with reduced feed efficiency. Fish growth was neither influenced by dietary Cu levels or by the dietary Cu history in any of the growth phases, but dietary Cu excess (Cu+) reduced body lipid and energy content in fry. In phase I, lower whole-body Zn concentration was observed in the fry of Zn- group compared to Zn+, while the contrary was observed in the juvenile fish as affected by dietary Zn history (phase III). In addition, Zn- dietary history showed increased levels of PUFA and higher mRNA expression of fatty acid biosynthesis genes in the liver. To conclude, early-stage dietary Zn+ history improved growth of juvenile rainbow trout, while dietary Zn- history exhibited signs of improved fatty acid biosynthesis capacity in the liver.

Effectiveness of Bacillus paramycoides for Improving Zinc Nutrition of Rice Irrigated with Alkali Water

Effectiveness of Bacillus paramycoides for Improving Zinc Nutrition of Rice Irrigated with Alkali Water

Modeling Zinc Absorption in the Adult Population of Colombia: Insights for Nutritional Evaluation and Intervention Strategies.

Zinc is a vital trace element, yet its deficiency is common in various populations. This study addresses the gap in understanding zinc intake and its relationship with key nutritional parameters in a Colombian population. We analyzed data from 12,987 individuals, focusing on the daily intake of zinc, phytate, protein, and calcium, and used the phytate/zinc molar ratio as an input parameter in the Miller et al. (2013) model. This model was employed to estimate the total absorbed zinc (TAZ) and the fractional absorption of zinc (FAZ). Our findings highlight a general trend towards insufficient intake compared to the standards of the Institute of Medicine (IOM) and Colombia, with a significant percentage of the population falling below the estimated average requirement (EAR) and recommended daily allowance (RDA) for zinc, underscoring the need for targeted nutritional strategies. Our study contributes to a broader understanding of zinc nutrition and public health implications in Colombia, providing a basis for future dietary guidelines and health interventions.

Plant-Microbe Interaction in Improving Zinc Nutrition in Rice: A Review

Zinc is an essential micro-nutrient that affects metabolic activities, including growth and cell proliferation in all living organisms. Zinc deficiency in agricultural soil has been increasing at an accelerated rate all over the world, leading to its deficiency in plants as well as humans. Zinc solubilising bacteria (ZSB) solubilise complex zinc in soil into plant absorbable compounds through several mechanisms such as the production of acid, chelating compounds, protons etc. further improving its bioavailability in plants and humans. Improving zinc nutrition through microbes is an effective measure to overcome its deficiency. ZSB with Plant Growth Promoting (PGP) traits can be an additional advantage as along with increasing zinc amount in plant it would also promote overall growth of plants through PGP traits and can act as a biocontrol agent against several crop pathogens. In this review we attempt to study the significance of zinc; status and deficiency of zinc in Indian soil and to understand how zinc solubilizing bacteria can prove to be an effective measure to increase zinc content in plants and overcome its deficiency.

Insights into the alleviation of cadmium toxicity in rice by nano-zinc and Serendipita indica: Modulation of stress-responsive gene expression and antioxidant defense system activation

The adversities of cadmium (Cd) contamination are quite distinguished among other heavy metals (HMs), and so is the efficacy of zinc (Zn) nutrition in mitigating Cd toxicity. Rice (Oryza sativa) crop, known for its ability to absorb HMs, inadvertently facilitates the bioaccumulation of Cd, posing a significant risk to both the plant itself and to humans consuming its edible parts, and damaging the environment as well. The use of nanoparticles, such as nano-zinc oxide (nZnO), to improve the nutritional quality of crops and combat the harmful effects of HMs, have gained substantial attention among scientists and farmers. While previous studies have explored the individual effects of nZnO or Serendipita indica (referred to as S.i) on Cd toxicity, the synergistic action of these two agents has not been thoroughly investigated. Therefore, the gift of nature, i.e., S. indica, was incorporated alongside nZnO (50 mg L−1) against Cd stress (15 μM L−1) and their alliance manifested as phenotypic level modifications in two rice genotypes (Heizhan43; Hz43 and Yinni801; Yi801). Antioxidant activities were enhanced, specifically peroxidase (61.5 and 122.5% in Yi801 and Hz43 roots, respectively), leading to a significant decrease in oxidative burst; moreover, Cd translocation was reduced (85% for Yi801 and 65.5% for Hz43 compared to Cd alone treatment). Microstructural study showed a decrease in number of vacuoles and starch granules with ameliorative treatments. Overall, plants treated with nZnO displayed gene expression pattern (particularly of ZIP genes), different from the ones with alone or combined S.i and Cd. Inferentially, the integration of nZnO and S.i holds great promise as an effective strategy for alleviating Cd toxicity in rice plants. By immobilizing Cd ions in the soil and promoting their detoxification, this novel approach contributes to environmental restoration and ensures food safety worldwide.

Zinc Nutrition in Rice Based Cropping Systems: A Comprehensive Review

Rice-based cropping systems being the most practiced cropping system in India, it suffers zinc deficiency as majority of the Indian soils are deficient in the Zn micronutrient. Zn is considered the fourth most important yield limiting nutrient of Agricultural crops. Apart from plant nutrition Zn also plays crucial role in human nutrition. Zn plays a vital role in the physiological function of plants and it is associated with phytohormone activity, protein synthesis and carbohydrate metabolism. To understand better zinc nutrition and its associated benefits from optimum nutrition and ways to manage Zn in rice-based cropping systems, the present review critically examines nutrient mobilization and transformation of Zn in rice-based cropping systems of India.

Comparative assessment of nano nitrogen and nano zinc nutrition on growth, yield and profitability of chilli (Capsicum annuum. L)

In contemporary agriculture, sustainability challenges like climate change, declining productivity, and nutrient deficiencies threaten food production. Judicious use of methodologies, dosages, and timing is crucial for sustainable practices. Nano-fertilizers gain importance due to their nutrient-release properties and increased use efficiencies. Chili is a commercial crop that requires high nutrients, the present field trial was carried out at AHRS, Bavikere, Shivmogga (India) during 2020 and 2021, to investigate its growth and yield responses to foliar applications of nano nitrogen (nN) and zinc (nZn) alongside conventional urea and ZnSO4. The experiment comprised combinations of recommended doses of nitrogen (RDN) (75, 100 & 125%) along with two foliar sources, nano (nN & nZn @ 0.4%) and conventional (urea @ 2% & ZnSO4@ 0.2%) against control. Foliar application of nN and nZn @0.4%+125% RDN (T3) improved growth and yield attributes & green chili yield (33.07 t ha−1), compared to RDF (T1, 23.8 t ha−1) and conventional sources (T6, 30.9 t ha−1). The utilization of nano-fertilizers in T3 resulted in a substantial enhancement of 39.0% in yield. Increments of 57.2% and 49.3% in net returns compared to conventional N and zinc sources respectively were also noted. Further, the land productivity reached $13.5 per day in T3, indicating economic viability. Application of 75% RDN with nN and nZn saved 25-50% of RDN compared to conventional sources. The results propose cutting nitrogen fertilizer by up to 50%, showcasing the eco-friendly aspect of nano-fertilizers. They boost productivity and profitability while supporting sustainable farming by reducing nitrogen usage.

Enzymatic alterations of soil and plant system on applied Zn sources, organics and microbial inoculants in zinc deficient calcareous and non-calcareous soils

The study aimed to investigate the impact of zinc (Zn) nutrition on enzymatic activity in soil and plant systems of zinc-deficient soils. Additionally, it aimed to understand the impact of solubilizer (such as Zinc Solubilizing Bacteria) and mobilizer (like AM Fungi) on enzyme activity and zinc nutrition in plants across calcareous and non-calcareous soil environments. A pot experiment was carried out with two different soils with three Zn sources (ZnSO4, ZnO and Zn-EDTA), organic manure (FYM) and microbial inoculants - Zinc Solubilizing Bacteria (ZSB) and Arbuscular Mycorrhizal Fungi (AM F). The DTPA-Zn and plant Zn content increased with applied sources of Zn. The microbial inoculants and FYM enhanced the use efficiency of all sources of Zn compared to unapplied treatments. Regarding soil enzyme activity, FYM and AM fungi played a major role which in turn altered the dehydrogenase (SDHA) and alkaline phosphatase (AlP) activities in both soils. The Zn sources and ZSB addition enhanced the plant enzyme activity. Calcareous soil recorded higher CA, SOD and alkaline phosphatase activities compared to non-calcareous soil the latter registered higher dehydrogenase activity and fruit Zn content.

Trade-Off Strategy for Usage of Phosphorus Fertilizer in Calcareous Soil-Grown Winter Wheat: Yield, Phosphorus Use Efficiency, and Zinc Nutrition Response

Although phosphorus (P) fertilizer application is widely used to improve production, irrational P application has a negative impact on the zinc (Zn) nutrition of cereal crops. Previous researchers observed and confirmed that P application decreases grain Zn concentrations and bioavailability in cereal crops. However, it remains unclear whether different P fertilizer types can alleviate the antagonism of P and Zn in the soil and grain and, thus, enhance the Zn nutritional level of cereal crops while maintaining production. Thus, a completely randomized pot experiment was conducted on winter wheat grown in two calcareous soils (lime concretion black soil and fluvo-aquic soil). Five P fertilizer types (single superphosphate, diammonium phosphate, fused calcium–magnesium phosphate, triple superphosphate, and ammonium polyphosphate, abbreviated, respectively, as SSP, DAP, FMP, TSP, and APP) were applied to each soil compared to no P fertilizer (CK). Plant and topsoil samples were collected during the flowering and maturity stages of winter wheat, and biomass, Zn concentrations in each organ, and grain phytic acid concentrations were analyzed. Grain yield was not affected by the application of different P fertilizer types to lime concretion black soil, while it was significantly increased by the application of TSP and APP to fluvo-aquic soil. The application of DAP and APP effectively promoted soil available Zn concentrations in both calcareous soils. In lime concretion black soil, the application of FMP significantly increased Zn remobilization to grains, while the application of DAP increased post-anthesis Zn uptake, thereby increasing grain Zn concentrations and its bioavailability. In fluvo-aquic soil, post-anthesis Zn remobilization and uptake were significantly increased by the application of TSP and APP, finally achieving higher grain Zn concentrations and Zn harvest index and effectively promoting grain Zn bioavailability. In conclusion, the rational application of DAP to wheat grown in lime concretion black soil and of TSP or APP to fluvo-aquic soil can achieve superior grain Zn nutrition quality while concurrently retaining high production and high P use efficiency, reducing micronutrient deficiency and further contributing to green agricultural development and human health.

Exploring Microbial Solutions: A Comprehensive Study on Isolating, Characterizing, and Selecting Zinc-Solubilizing Fungi from Rhizospheric Soil

This study investigates the zinc solubilization potential of fungal isolates and their antagonistic activity against rice pathogens to address zinc deficiency and disease incidence in rice cultivation. Rhizospheric soil samples were collected, and zinc-solubilizing fungi were isolated and purified. Molecular characterization identified Talaromyces sp, Talaromyces versatilis, Talaromyces pinophilus, and Aspergillus terreus as zinc solubilizers. Qualitative and quantitative assays revealed varying solubilization efficiencies among isolates over time, with Talaromyces versatilis exhibiting the highest zinc solubilization. In inhibition assays conducted against rice pathogens, fungal isolates exhibited antagonistic potential, with Talaromyces versatilis demonstrating the highest percentage of inhibition. These findings underscore the potential of fungi as bio-based solutions for improving zinc nutrition and disease management in rice cultivation.

Dietary Supplementation of Tannins: Effect on Growth Performance, Serum Antioxidant Capacity, and Immunoglobins of Weaned Piglets-A Systematic Review with Meta-Analysis.

In recent years, the swine industry has witnessed the withdrawal of antibiotics and continuous regulation of zinc and copper oxides in the early-life nutrition of piglets. Due to this development, alternative additives from plant sources have been extensively explored. Therefore, this study's objective was to evaluate the effect of dietary supplementation with tannins on weaned piglets' growth performance, serum antioxidant capacity, and serum immune status using a systematic review and meta-analysis approach. A total of 16 studies with parameters of interest were deemed eligible after a two-step screening process following a comprehensive literature search in the scientific databases of Web of Science, Scopus, ScienceDirect, PubMed, and Google Scholar. The inclusion criteria were mainly (1) studies involving basal diet supplemented with tannins and (2) studies with the quantification of tannin doses, while the exclusion criteria were (1) studies with pre- and post-weaning pigs and (2) challenged studies. Applying the random-effects models, Hedges' g effect size of supplementation with tannins was calculated using R software to determine the standardized mean difference (SMD) at a 95% confidence interval. Sub-group analysis and meta-regression further explored heterogeneity (PSMD < 0.05, I2 > 50%, n ≥ 10). Supplementation with tannins reduced the feed conversion ratio (p < 0.01) but increased the final body weight (p < 0.01) of weaned piglets. Chestnut and grape seed proanthocyanidin tannin sources yielded higher effects on growth performance. In addition, meta-regression models indicated that tannin dosage and supplementation duration were directly associated with tannins' effectiveness on productive performance. In the serum, the concentration of glutathione peroxidase, superoxide dismutase, and total antioxidant capacity were elevated (p < 0.01) in response to tannin supplementation, whereas malondialdehydes was reduced (p < 0.01). Likewise, increased immunoglobin M and G levels (p < 0.01) were detected. In conclusion, dietary supplementation with tannins, particularly with chestnut and grape seed proanthocyanidins, increases the productivity of weaned piglets. At the same time, it is a possible nutritional strategy to mitigate oxidative stress and stimulate gut health. Thus, supplementing chestnut and grape seed proanthocyanidin tannins in the early phase of swine production could be used to alleviate the incidence of diarrhea.

Electropolymerization of poly(phenol red) on laser-induced graphene electrode enhanced adsorption of zinc for electrochemical detection

We present a highly sensitive and selective electrode of laser-induced graphene modified with poly(phenol red) (P(PhR)@LIG) for measuring zinc nutrition in rice grains using square wave anodic stripping voltammetry (SWASV). The physicochemical properties of P(PhR)@LIG were investigated with scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), Fourier infrared spectroscopy (FT-IR) and Raman spectroscopy. The modified electrode demonstrated an amplified anodic stripping response of Zn2+ due to the electropolymerization of P(PhR), which enhanced analyte adsorption during the accumulation step of SWASV. Under optimized parameters, the developed sensor provided a linear range from 30 to 3000 μg L−1 with a detection limit of 14.5 μg L−1. The proposed electrode demonstrated good reproducibility and good anti-interference properties. The sensor detected zinc nutrition in rice grain samples with good accuracy and the results were consistent with the standard ICP-OES method.

Nutrient impact: Unveiling the growth and biochemical dynamics of Kalmegh (Andrographis paniculata Nees.) through zinc and iron nutrition

Nutrient impact: Unveiling the growth and biochemical dynamics of Kalmegh (Andrographis paniculata Nees.) through zinc and iron nutrition