Sediment cores were collected from Wrong (WL) and Lenore (LL) lakes in boreal central Yukon Territory, Canada, spanning circa 6000 years. Analyses of pollen, charcoal, magnetic susceptibility, levoglucosan, and down-scaled paleoclimatic data reveal the vegetation history and character/drivers of Holocene fire disturbance. Boreal forest has persisted in the region for millennia, with a regional mid-Holocene expansion of Picea mariana followed by expansion of Pinus contorta in the latest Holocene. The shortest reconstructed fire return intervals (FRI; ca. 110-125 years) occurred in the mid-Holocene, coincident with the development of highly flammable Picea-dominated forest and warm, dry summers that were characterized by elevated JJA Z500 anomalies and associated with an eastward/intense Aleutian Low. In the late-Holocene, FRI increased to ca. 240 years at WL and 280 years LL. Drivers of this change include regional cooling and increased precipitation, with a westward/weaker Aleutian Low position delivering more moisture to interior Yukon. Longer FRIs allowed for greater fuel accumulation between fires, enhancing fire size/severity. While higher fire frequency is noted in the mid-Holocene and increased fire size/severity in the late-Holocene, recent changes in Yukon fire disturbance suggest that the fire regime may soon lie outside the natural range of mid- and late-Holocene variability.