Yellow Vein Mosaic Disease Research Articles

Begomovirus disease of pumpkin crop in India and its management strategies possibility: a review

Pumpkin is an important commercial crop grown worldwide in tropical and subtropical regions. The whitefly-transmitted Pumpkin yellow vein mosaic disease seriously threatens pumpkin cultivation worldwide. The advent of transgenic technology in the 1980s revolutionized the possibilities for introducing virus resistance into agriculturally important plant species. It offered a powerful tool to enhance crop protection and provided a pathway to potentially unlimited sources of resistance against viral diseases. The ongoing research and development in this field continue to explore and refine conventional and non-conventional approaches for effective virus management in agriculture crops. This review focuses on developing transgenic resistance against begomoviruses and discusses possible management strategies to address these challenges.

Designing of an Innovative Multiplex Model to Control Insects/Pests (Whitefly) in Okra Crop Fields and Assessment for its Efficacy in Control Viral Diseases

Whitefly (Bemisia tabaci) destroys a great proportion of crops in India. Whitefly is the carrier of begomovirus which causes okra yellow vein mosaic viral disease. Preventing the pest infestation without the use of chemical fertilizers and pesticides is the need of the hour. Integrated Pest Management (IPM) involves strategies that maintain pest population below Economic Injury Level (EIL). In the present study a multiplex insect catching trap was constructed with the aim of controlling pest infestation in a sustainable and eco-friendly manner. The multiplex trap was prepared using yellow sticky papers and Methyl Eugenol pheromone as an insect/pest attractant. The traps were placed in field and thereafter plants were tested for the presence of viral DNA using PCR. The fields in which traps were placed showed negligible or few instances of viral infection while the fields in which no traps were placed showed considerable virus infection. Additionally, the fields with the trap showed a higher yield percentage of crop compared to the field with no trap.

First record of tomato leaf curl Kerala virus and betasatellite associated with yellow vein mosaic disease of beet root (Beta vulgaris L. ssp. vulgaris) in India

First record of tomato leaf curl Kerala virus and betasatellite associated with yellow vein mosaic disease of beet root (Beta vulgaris L. ssp. vulgaris) in India

Agronomic evaluation of introgressed lines developed for yellow vein mosaic disease resistance in okra (Abelmoschus esculentus L. Moench)

Agronomic evaluation of introgressed lines developed for yellow vein mosaic disease resistance in okra (Abelmoschus esculentus L. Moench)

Relative toxicity of some newer insecticide molecules against vector and sucking pest complex of okra

Sucking pest like leaf hoppers or jassid (Amrasca biguttula biguttula (Ishida)) and vectors i.e., whitefly (Bemisia tabaci (Gennadius)) are regarded as one of the major biotic stresses in okra cultivation in India. Apart from direct sucking the plant sap and thereby devitalizing the crop through their specially adapted mouthparts, many of them also act as vectors for important plant diseases like okra yellow vein mosaic and okra enation leaf curl disease. To control these sucking pests and vectors, several newer insecticide molecules were tested under open field conditions during Kharif seasons of 2021 and 2022. Based up on the two years of pooled data, among the tested insecticides, Tolfenpyrad 15% EC @ 2 ml/L was the best molecule in reducing leaf hopper population in okra (5.40 per leaf) with 63.49% reduction over control (PROC). The next best molecule was flupyradifurone 17.09% SL (5.70 jassids per leaf with 61.46 PROC). In the case of whitefly, flupyradifurone 17.09% SL was the most promising during both years with percent population reductions of 69.90 over control. The next best molecule in the list was tolfenpyrad 15% EC with 67.08 PROC. Interestingly, Imidacloprid-treated plots had the highest leaf hopper (10.39 per leaf) and whitefly (9.55 per leaf) population among all treatments indicating its low activity against these sucking pests of okra. The highest healthy green fruit yields (152 q/ha) were obtained from the plots treated with tolfenpyrad 15% EC with a maximum cost-benefit ratio of 1:2.19 followed by dinotefuran 20% SG (1:2.16) and acetamiprid 20% SP (1:2.15).

Prevalence of Okra Yellow Vein Mosaic Viral Disease and its Correlation with Vector Population in Sindh, Pakistan

Okra (Abelmoschus esculentus (L.) Moench) being an important vegetable crop is widely grown in tropical and subtropical regions of the world. Whitefly (Bemisia tabaci) is not only a major insect pest of okra in Pakistan but also the vector insect of Begomoviruses including okra yellow vein mosaic virus which causes okra yellow vein mosaic viral disease (OYVMVD). Regional monitoring of insect vectors and disease prevalence is being emphasized for proper management, therefore this study was focused on determining the intensity of OYVMVD in the fields and the correlation between the disease and vector population. For this purpose, the virus infection was confirmed with DNA sequencing. The disease and vector population was visually assessed approximately 65-70 days after sowing of okra under field conditions at commercial farmer fields in district viz., Hyderabad, Tando Allahyar, Matiari, and Sanghar of Sindh, Pakistan during the growing season of 2016. Ten okra plants with three replications (N = 30) at ten different locations of each district were assessed. Results of nucleotide alignment of DNA amplified from diseased plants confirmed that yellowing and vein clearing were due to the virus infection. The visual assessment showed that the disease was prevailing throughout all region monitored in this study and the disease was significantly correlated with the vectors population. However, maximum disease incidence and severity were recorded in Tando Allahyar. Therefore, it is recommended to validate various management practices at such high disease risk points for better management

Identification of SSR markers through bulk segregant analysis and inheritance of resistance to yellow vein mosaic disease in okra (Abelmoschus esculentus L. Moench).

The online version contains supplementary material available at 10.1007/s13337-023-00844-9.

Field survey and molecular characterization of a bipartite begomovirus causing okra yellow vein mosaic virus disease in the Rajshahi region of Bangladesh

Field survey and molecular characterization of a bipartite begomovirus causing okra yellow vein mosaic virus disease in the Rajshahi region of Bangladesh

Performance of okra backcross derivatives for agronomic traits and yellow vein mosaic disease resistance

Performance of okra backcross derivatives for agronomic traits and yellow vein mosaic disease resistance

Identification of novel sources of yellow vein mosaic disease resistance in okra (Abelmoschus esculentus L. Moench)

Identification of novel sources of yellow vein mosaic disease resistance in okra (Abelmoschus esculentus L. Moench)

First report of a begomovirus and associated betasatellite causing yellow vein mosaic disease of Celosia cristata

First report of a begomovirus and associated betasatellite causing yellow vein mosaic disease of <i>Celosia cristata</i>

Virus–vector relationship of squash leaf curl China virus and its transmission through whitefly vector, Bemisia tabaci (Gennadius) causing yellow vein mosaic disease of pumpkin

Virus–vector relationship of squash leaf curl China virus and its transmission through whitefly vector, Bemisia tabaci (Gennadius) causing yellow vein mosaic disease of pumpkin

Impact assessment of natural variations in different weather factors on the incidence of whitefly, Bemisia tabaci Genn. and yellow vein mosaic disease in Abelmoschus esculentus (L.) Moench

Bemisia tabaci Gennadius, also renowned as the silver leaf whitefly, is among the most damaging polyphagous insect pests in many commercially important crops and commodities. A set of field experiments were conducted for three consecutive years i.e., 2018–2020, to investigate the role of variations in rainfall, temperature, and relative humidity on the abundance of B. tabaci in okra (Abelmoschus esculentus L. Moench). In the first experiment, the variety Arka Anamika was cultivated twice a year to analyse the incidence of B. tabaci concerning the prevailing weather factors and the overall pooled incidence recorded during the dry and wet season was 1.34 ± 0.51 to 20.03 ± 1.42 and 2.26 ± 1.08 to 18.3 ± 1.96, respectively. Similarly, it was observed that the highest number of B. tabaci catch (19.51 ± 1.64 whiteflies/3 leaves) was recorded in morning hours between 08:31 to 09:30 a.m. The Yellow Vein Mosaic Disease (YVMD) is a devastating disease of okra caused by begomovirus, for which B. tabaci acts as a vector. In another experiment, screening was conducted to check the relative susceptibility of three different varieties viz., ArkaAnamika, PusaSawani, and ParbhaniKranti against B. tabaci (incidence) and YVMD ((Percent Disease Incidence (PDI), Disease Severity Index (DSI), and Area Under the Disease Progress Curve (AUDPC)). The recorded data was normalized by standard transformation and subjected to ANOVA for population dynamics and PDI. Pearson's rank correlation matrix and Principal Component Analysis (PCA) have been used to relate the influences of various weather conditions on distribution and abundance. SPSS and R software were used to create the regression model for predicting the population of B. tabaci. Late sown PusaSawani evolved as a highly susceptible variety in terms of B. tabaci (24.83 ± 6.79 adults/3leaves; mean ± SE; N = 10) as well as YVMD i.e., PDI (38.00 ± 4.95 infected plants/50plants), DSI (71.6–96.4% at 30 DAS) and AUDPC (mean β-value = 0.76; R2 = 0.96) while early sown Parbhani Kranti least susceptible to both. However, the variety ArkaAnamika was observed as moderately susceptible to B. tabaci and its resultant disease. Moreover, environmental factors were predominantly responsible for regulating the population of insect pests in the field and hence its productivity like rainfall and relative humidity were negative while the temperature was positively correlated with B. tabaci (incidence) and YVMD (AUDPC). The findings are helpful for the farmers to choose need-based IPM strategies than timing-based, which would fit perfectly with the present agro-ecosystems in all ways.

Evaluation of F1 hybrids for horticultural and quality traits and pumpkin yellow vein mosaic disease resistance in pumpkin

Pumpkins being cheap and having higher medicinal value can act as a saviour to low-income families. Butbeing a minor crop, little attention has been paid to its improvement. The hybridization in pumpkins is economicallyfeasible due to the monoecious nature and higher number of seeds per fruit. Moreover, the development of superiorhybrids can enhance production and consumption through ease in cultivation, meeting consumers’ preferencesand suitability to different seasons. Hitherto, 34 F1 hybrids were evaluated during spring and rainy seasons forstandard heterosis. H9 (P-225 × GFR-6783) and H28 (VR-1365 × VR-13364) hybrids exhibited maximum standardheterosis for yield per plant and can be exploited for commercial cultivation. Resistance to PYVMD is a pre-requisitefor the successful cultivation of pumpkin in the rainy season. 11 hybrids (H24, H25, H26, H27, H28, H29, H30, H31,H32, H33 and H34) tolerant to PYVMD offered the advantage of cultivating pumpkin in both seasons.

Molecular characterization of monopartite bhendi (Abelmoschus moschatus) yellow vein mosaic virus and screening of wild okra

Bhendi yellow vein mosaic disease (BYVMD) is the most devastating viral disease of okra (Abelmoschus moschatus ssp. moschatus) which affects yield and quality of the produce in India. The causative agent of BYVMD is begomovirus i.e. bhendi yellow vein mosaic virus (BYVMV) having DNA-A molecule along with betasatellite. Present study was carried out during 2019–21 at research farm of ICAR-National Bureau of Plant Genetic Resources, New Delhi to find out the diversity of begomovirus and its satellite nucleotide sequences derived from wild okra infected samples exhibiting conspicuous symptom of BYVMD using PCR-based detection technique on two wild okra symptomatic samples, viz. EC361170 and EC361148. Full length amplification of BYVMV satellite molecule and partial amplification of DNA-A was carried out using PCR and cloning of both randomly selected samples showed the presence of monopartite BYVMV. In both samples, presence of DNA-A molecule, betasatellite and alphasatellite were noticed. Two year (kharif 2019 and 2021) field screening of 10 accessions of wild okra (Abelmoschus moschatus ssp. moschatus) was carried out at ICAR-NBPGR, New Delhi. Out of 10 accessions, viz. EC360586, EC360794, EC360830, EC360900, EC359730, EC359836, EC359870, EC360351, EC361111 and EC361171 screened, 4 accessions, viz. EC360794, EC360586, EC360830 and EC361171 showed resistant (R) reaction during both the seasons against BYVMD. This is the first study which showed the presence of alpha-satellite molecule of BYVMV from New Delhi region in wild okra along with its resistance source.

Grower’s Guide: A Review for Sustainable Production of Okra (Abelmoschus Esculentus) in West Africa and Other Regions

Abstract: This paper provides detailed information specifically the botany, history, and current statistical report on okra as well as how it is cultivated. Major biotic and abiotic stress factors impeding the production of okra and the sustainability aspect in the production of the crop are discussed. Many reasons for poor growth and development, low yield of okra as well as seed dormancy or okra plant death are mentioned. These include poor quality of seed and some biotic stresses particularly yellow vein mosaic virus (YVMV) and abiotic stresses such as salinity, drought, various water stresses and high moisture content. However, okra as a tropical and subtropical crop is drought-resistant, thus does well under drought conditions. Notwithstanding, high moisture content, salinity, and the yellow vein mosaic virus disease are the major factors affecting the crop. These conditions especially the yellow vein mosaic virus disease reduce okra yield to about 94% depending on the time of infestation after germination. To control the mosaic virus disease, very limited success has been achieved by chemical methods (including the application of neem oil, Acetamiprid, Imidacloprid and Trizophos), which also are not permanent since some resistant cultivar become susceptible with time. Therefore, advance breeding and development of host resistance to viruses and measures to combat salinity are the important strategies against these impeding conditions.

EFFECT OF YELLOW VEIN MOSAIC VIRUS DISEASE OF OKRA ON GROWTH AND PROXIMATE ANALYSIS OF SOME OKRA CULTIVARS IN DUTSIN-MA

The study was conducted on okra, an important food consumed in Dutsin ma, Katsina State, Nigeria. Reports have indicates the effect of viral infections on produce quantity and quality, this study determined and document the effect of yellow vein mosaic virus disease of okra on performance and nutritional composition of some okra cultivars in Dutsin-ma, Sudan Savannah. Seedlings were inoculated with the virus at 1 week post emergence. Each cultivar was evaluated as inoculated (infected) and uninoculated (Healthy) treatments. Percent disease severity of infection (%), yield related parameters and proximate analysis of pod were measured. Data were subjected to independent t test and significance was determined at 5 % level of probability. Percent disease severity was highest in ‘Yar Balla’ (21.3%) as compared to the remaining cultivars. Reductions in plant height (8.8 %), fruit weight (37.6 %) and crude protein were significantly lower in ‘Yar Balla’ and ‘Jikin Mutum’ The present study reveal that the reduction in crude protein as a result of YVMV infection would worsen the protein deficiency problem of the populace who depend on the crop as the cheapest means of obtaining their daily protein and mineral requirement. There is therefore the need to prevent viral infection of vegetable crops in order to ensure high yield and guarantee its nutritive value.

Molecular characterization and identification of bhendi yellow vein mosaic virus with satellite DNAs infecting okra plants of district Mardan, Pakistan

Abstract Background Bhendi or okra (Abelmoschus esculentus) is an important crop widely cultivated in the Indian subcontinent. The production of okra in tropical regions is constrained by several abiotic and biotic factors. Among biotic stresses, yellow vein mosaic disease (YVMD), transmitted by the whitefly (Bemisia tabaci Genn.), causes significant production losses. Results Leaves showing symptoms of YVMD were collected from okra plants in the district of Mardan, Pakistan, from which the total nucleic acid was extracted. It was found that the viral genome was 2739 bp in the length and had seven conserved open reading frames, comparable to those of monopartite begomovirus species from the Old World. Nucleotide sequence comparison revealed that the genome has 97.7% identity with bhendi yellow vein mosaic virus (BYVMV). Additionally, alpha- and betasatellite DNA components were amplified and sequenced. The alpha- and betasatellite DNA sequences were 1367 and 1346 nt in length, respectively. Sequence analysis revealed that the alpha- and betasatellite sequences shared 97.9 and 98.7% similarity with cotton leaf curl Multan alphasatellite DNA (CLCuMuA) and croton yellow vein mosaic betasatellite DNA (CroYVMB), respectively. Conclusions Based on the prevailing classification system, the isolate was identified as a variant of BYVMV, CLCuMuA, and CroYVB. Alphasatellite presence in the begomovirus betasatellite complex detected in the present study indicated a recent mobilization into the viral complex infecting okra in this region. The study findings may facilitate the design of new management strategies to protect this valuable crop against begomovirus infection.

Copy number variation of two begomovirus acquired and inoculated by different cryptic species of whitefly, Bemisia tabaci in Okra.

The whitefly, B.tabaci is a major pest of agricultural crops which transmits begomovirus in a species-specific manner. Yellow vein mosaic disease (YVMD) and okra leaf curl disease (OLCD) caused by distinct begomovirus are a major limitation to production of okra in India. In this framework the present investigation reports, for the first time, comparative study of begomovirus species viz. yellow vein mosaic virus (YVMV) and okra enation leaf curl virus (OELCuV) ingested and egested by two cryptic species (Asia I and Asia II 5) of B.tabaci at different time interval using detached leaf assay. A gradual increase of both virus copies were observed with increased feeding exposure in Asia I and Asia II 5. Both the genetic groups of whitefly could acquire the viruses within just 5 minutes of active feeding however, a significant amount of variation was noted in virus uptake by the both. At 24 hours of active feeding Asia II 5 acquired more of YVMV whereas, Asia I ingested more OELCuV. Similarly, the genetic group acquiring higher titre of virus egested higher amount during inoculation period. On the whole, it can be presumed that Asia I is a more effective transmitter of OELCuV whereas, Asia II 5 of YVMV further suggesting increased risk of virus pandemics (both YVMV and OELCuV) in regions where Asia I and Asia II 5 is dominant.

Evaluation of okra genotypes against yellow vein mosaic disease (YVMD)

Evaluation of okra genotypes against yellow vein mosaic disease (YVMD)