Yellow-fleshed Cassava Root Research Articles

Variations in micronutrient concentrations and retentions in fufu made from yellow-fleshed cassava as a function of genotype and processing methods.

The biofortification of staple foods such as cassava is one of the technological breakthroughs in the nutritional improvement of foods. Fufu is one of the fermented cassava products produced and consumed in major West African countries, including Sierra Leone, and the majority of the processes involved in its production have direct and indirect effects on its properties. This study looked at how the concentration and retention of micronutrients in yellow-fleshed cassava fufu varied depending on genotype and processing method. Six yellow-fleshed cassava root genotypes (TMS-070557, TMS-011371, TMS-011412, TMS-011663, TMS-083724, TMS-083774) and one white (TME 419 as a control) were processed into fufu using both conventional (oven and sun-dried) and traditional (bowl and river) methods. The Statistical Analysis System (SAS) version 9.4 was used to analyze data using means, percentages, analysis of variance and means separated by least significant differences (LSD). In the modified traditional river method, raw and cooked fufu samples had significantly higher β-carotene concentrations and true retention (TR) percentages (11.06 g/g (46.77%) and 4.54 g/g (16.94%), respectively) than other genotypes (p < 0.0001). Modified traditional fufu processing methods increased total β-carotene concentrations, while raw roots showed a significant decrease in total carotenoid and β-carotene concentrations, regardless of genotype or processing method. Sun-drying was the most effective method, with significantly higher concentrations and TR percentages of iron (10.01 mg/kg, 18.02%) and zinc (11.49 mg/kg, 40.64%) in raw and cooked fufu samples. Genotype TMS-083724 outperformed both conventional fufu processing methods, displaying a significant total carotenoid concentration and true retention percentage. Finally, this study found that the concentrations and percentages of TR of micronutrients varied depending on the processing method and genotype. It is recommended that a modified traditional river fufu processing method be further developed and improved in order to maximize provitamin A carotenoids, concentrations, and percentage TR.

Influence of traditional processing and genotypes on the antioxidant and antihyperglycaemic activities of yellow-fleshed cassava.

Yellow-fleshed cassava root (YFCR) is processed into traditional products that may influence its bioactivities. In this study, the antioxidant and anti-hyperglycaemic activities of three traditional products (lafun, fufu and gari) from five genotypes (IITA-TMS-IBA070337, 182961, 182962, 182986, 183044) of YFCR were evaluated. The YFCR genotypes were grown at the International Institute of Tropical Agriculture (IITA) research field, Ibadan. The bioactive constituents (total carotenoids, total phenolics, tannins and total flavonoids), antioxidant [2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) radical cation (ABTS•+) and 1,1-diphenyl-2- picrylhydrazyl radical (DPPH•) scavenging capacities, and reducing power], and starch-digesting enzymes (α-amylase and α-glucosidase) inhibitory activities of the products were determined using standard laboratory methods. The glucose response of the products was assessed in human subjects. The concentrations of the bioactive constituents of the products from different genotypes varied significantly (p < 0.05). The ABTS•+ and DPPH• scavenging capacities and the reducing power of the products also differed significantly (p < 0.05), such that the lafun from IITA-TMS-IBA182962, IITA-TMS-IBA070337 and IITA-TMS-IBA070337 had the strongest ABTS•+ and DPPH• scavenging capacities, and reducing power, respectively. The α-amylase and α-glucosidase inhibitory activities of the three products differed significantly (p < 0.05), with the lafun from IITA-TMS-IBA070337 and IITA-TMS-IBA07033 having the strongest α-amylase and α-glucosidase inhibitory activity, respectively. Also, the lafun from IITA-TMS-182986 had the least glucose response, while the fufu from IITA-TMS-IBA070337 had the highest glucose response. Overall, the lafun from different genotypes of YFCR had the most potent antioxidant and starch-digesting enzymes inhibitory activities and the least glucose responses. Hence, lafun may be a promising dietary intervention targeting oxidative stress, hyperglycaemia, and their resultant type 2 diabetes.

Characterization of some quality attributes of vacuum fried yellow fleshed cassava chips from different varieties using designed experiment

A two-level full factorial design was used to explore the influence of process conditions on the qualities of fried chips from TMS 01-1368, TMS 01-1371, and TMS 01-1412 cultivars of yellow fleshed cassava roots under vacuum. Frying temperature (122℃ and 129℃), frying time (8 and 10 min), and vacuum pressure (9.91 and 14.91 cmHg) were used to evaluate changes in color, texture, shrinkage, moisture, oil, and carotenoid contents. Frying temperature, vacuum pressure, and frying time at linear, quadratic, and interaction terms showed significant (p < .05) effect on the qualities and carotenoid contents of fried chips from yellow fleshed cassava cultivars. The coefficients of determination (R2) of all responses were > 0.75. Increased frying temperature and time reduced the L* value. The optimized solutions for fried chips are 129℃/9.91 cmHg/9.57 min for TMS 01-1371; 122℃/14.49 cmHg/8 min for TMS 01-1371; 122℃/ 9.91 cmHg/9.95 min for TMS 01-1412. Novelty impact statement Classical experimental design was used to optimize vacuum frying processing of yellow fleshed cassava roots. Different optimized vacuum frying processing conditions were obtained for three varieties. Vacuum fried chips were superior to the ones fried under atmospheric condition with minimum oil content, maximum textural properties, and carotenoid contents. Fried chips from TMS 01-1368 had lower oil content and breaking force value than others.

Evaluation of qualities of extruded snacks from yellow cassava flour substituted with processed sesame seed’s flour

Advocacy on the utilization of locally grown crops keeps increasing. This study was however carried out to evaluate the qualities of extruded snacks from blends of yellow cassava and sesame seeds flour. The yellow fleshed cassava roots and sesame seeds (germinated and fermented) were dried, milled and sieved prior to further analysis. Flour of each of the processed sesame seed was blended with yellow cassava flour at substitution levels of 0, 15 and 30%. Functional and anti-nutritional analyses were carried out on the flour blends. The flour blends were mixed thoroughly to produce extruded snacks in a single screw extruder before frying in a deep fryer. Anti-nutrient, proximate and sensory evaluations were carried out on the extruded snacks. There was considerable increase in the functional and proximate compositions with an increase in sesame seed flour incorporation most especially samples with 30% fermented sesame seeds flours, but with a considerably low anti-nutrient in the extrudates. Extruded samples with 30% Fermented sesame seed flour also had the highest (7.20) rating with regards to the overall acceptability closely followed by extruded snacks from 100% yellow cassava flour (7.05) and samples with 30% germinated sesame seeds flour. The results indicated significant differences (p&lt;0.05) among the samples. This research revealed that extruded snacks can be produced from non-wheat flours which will reduce over dependence on imported wheat and hence increase the utilization of locally grown crops. It will contribute to large scale production and utilization of the crops for enhanced national food security.

Quantitative Assessment of Trace and Macro Element Compositions of Cassava (Manihot esculenta) Storage Roots Enriched with Β-Carotene as Influenced by Genotypes and Growing Locations

Cassava’s important mineral contents depends on some factors, including genetic and growing locational factors. The study aimed to evaluate the influence of genotype and growing locations on the mineral concentrations in yellow-fleshed cassava root genotypes. Twenty-five pipeline yellow-fleshed cassava genotypes and three white-fleshed varieties (check samples) were planted at five different experimental fields for two seasons, each representing the major agroecological zones in Nigeria. Standard laboratory protocols were employed in the sampling to ensure zero contamination, and the trace and macro elements were determined using the inductively coupled plasma optical emission spectroscopic method (ICPOES). The trace and macro elements identified in all the genotypes and varieties investigated were Fe, Mn, B, Cu, Mo, Co, Ni, Zn, and Al; Ca, Mg, Na, K. P, and S respectively. Genotype and growing location had a highly significant (p &lt; 0.05) effect on all the trace elements except Ti and Cr. However, there was no interactive effect between genotype and growing location on all the trace elements except for Pb and Zn. Among the explanatory variables, the variable growing location was the most influential on macro and trace elements. Conclusively, genotypes 01/1442 and 01/1273 have outstanding trace and macro element concentrations.

Variations of Macro- and Microelements in Yellow-Fleshed Cassava (Manihot esculenta Crantz) Genotypes as a Function of Storage Root Portion, Harvesting Time, and Sampling Method

The correct estimation of the mineral content of cassava (Manihot esculenta) genotypes is vital from a nutritional point of view. This study evaluated the effects of the storage root section, maturity, and sampling method on the macro- and microelements in yellow-fleshed cassava root genotypes. In total, 44 genotypes were grown in replicated field trials of 2 sets (set 25 and set 19) and were harvested at 9 and 12 months after planting. Two sampling methods, sampling with a cork borer (A = proximal, B = middle, C = distal or method 1) and sampling without a cork borer (L = Longitudinal or method 2), were used. The minerals of the samples from the two methods were determined using inductively coupled optical emission spectrometry (ICP-OES). K and Mn were the most abundant minerals, and Na, Mo, and Co were the least abundant. Genotype, method, and maturity had a strong influence on mineral concentrations. Harvesting time affected the concentration level of some macro- and microelements in cassava roots. Additionally, Ca, Mg, K, P, Mn, Cu, Ni, and Zn contents were significantly (p &lt; 0.05) higher in the proximal and middle portions for method 1. K and P and Mn and B were positively correlated, but K and Na and Fe and Ni were negatively associated.

Impact of Processing and Genotype on Concentration and Bioaccessibility of Fe in Fufu Produced from Yellow-Fleshed Cassava (Manihot esculenta crantz) Roots (P02-010-19)

ObjectivesFe deficiency is relatively high in areas that grow and consumed cassava as staple food. This study investigated the Fe concentration and bioaccessibility in fufu produced from yellow-fleshed cassava roots genotypes. MethodsSix genotypes of yellow-fleshed and one genotype of white cassava roots were processed into fufu using conventional (oven and sun dried) and traditional (bowl and river) methods and the samples were analyzed for Fe using standard laboratory procedures. Data were analyzed using means which were then separated by Duncan’s multiple range tests. ResultsResult shows significant differences (P < 0.05) in mean Fe concentration (ranged from 6.67–13.22 μg/g in cooked fufu per dried weight) with sun-dried fufu recording the highest value and the Traditional Bowl fufu recording the least value. Genotype 3724 scored the highest while SLICASS 7 scored the least Fe concentration. The in-vitro result revealed a strong ferritin formation in traditional river fufu than sun-dried fufu. Indeed, these were identified in higher levels in the sun-dried fufu than all the other fufu samples. ConclusionsThis result indicates both processing and genotype affect Fe and further identifies cassava roots potential to enhancing Fe nutrition in populations that produced and consumed this product. Funding SourcesWest African Agricultural Productivity Program 1c_Sierra Leone, Normman Borlaug Leadership Enhancment Agricultural Program, Sierra Leone Agricultural Research Institute.

Impact of Cowpea and Broad Bean Fortification on the Proximate, Functional and Sensory Properties of Cassava Based Fermented Product (Gari) (P04-032-19)

ObjectivesThe objective of this study was to improve the nutritional value of gari with legumes found in Sierra Leone. Hence, the need for Fortification with legumes. MethodsGari from white and yellow-fleshed cassava root were fortified separately with cowpea and broad bean paste in proportions ranging from 0 to 40% and evaluated for proximate, functional and sensory characteristics using standard laboratory methods. ResultsResults showed that protein, fat and ash content increased with an increasing proportion of bean paste. Protein content of the product also increased significantly (P < 0.05) from 1.38% to 9.27%, while the starch content decreased from 95.01% to 69.51%. Swelling power ranged from 12.36% to 7.05% at 20% level of fortification substitution with cowpea or broad beans paste. The 20% cowpea-fortified white root gari was the most preferred in terms of aroma (4.23), taste (4.89) and chewiness (3.98), while 20% cowpea- fortified yellow root gari rated highest for colour (4.27). Generally, 20% both yellow and white root gari fortified with 20% were accepted than those of 40% substitution. ConclusionsConclusion, nutritional values of both gari increased with increasing level of fortification with of beans. Therefore the 20% fortified gari being most preferred is recommended to improved nutritional status of gari consumers. Funding SourcesSierra Leone Agricultural Research Institute.

Retention of pro-vitamin A carotenoid in composite bread baked with high quality cassava flour from yellow-fleshed cassava root

Background: As one of the most widely consumed foods, bread is one of the most important agricultural products. Bread made from high-quality cassava flour is consumed in some parts of Sub-Sahara Africa (SSA). The bread has no pro-vitamin S carotenoids (pVAC) due to the use of artificial colorants. Consequently, there is a need for the use of pVAC rich foods for bread production. Foods that are rich with pro-vitamin A carotenoids can be converted into retinol in the human body and whose bioconversion contributes to the reduction of vitamin A deficiency diseases (VAD). VAD has caused annual loss of life in SSA, especially in Nigeria. The yellow-fleshed cassava root might contribute to the reduction of this disease. The high quality yellow cassava flour (YHQCF) produced from yellow-fleshed cassava root may contribute to the pVAC composition of bread. As a result, there is a need for the evaluation of the retention of pVAC in composite bread baked with high quality cassava flour from yellow-fleshed cassava roots. Methods: The YHQCF was produced from TMS01/1368 cassava variety. The bread loaves consisted of 20% and 100% YHQCF and were produced by mixing the sugar, margarine, yeast, improver, and salt with the composite flour and YHQCF respectively, after which water was added and mixed to get the homogenous dough. The dough was proofed for 2.5 hours, kneaded, cut into shape, placed in a lubricated baking pan, and baked at 200oC for 30 min. Analyses of the pro-vitamin A (cis and trans-β carotene) and dry matter content were carried out on all the samples, including samples from the YHQCF production steps using standard methods. The samples from the YHQCF production steps were chosen and analyzed for pVAC in order to check the levels of degradation of the pVAC from the raw cassava root to using the root for flour production and the quantity of pVAC retained when 100% of the YHQCF is used for bread production compared to 20% composite. The β-carotene nutrient retention of the bread was also calculated.Results: The results demonstrated how the total pVAC content of the raw yellow-fleshed cassava root was 16.83 µg/g dry basis with 29% dry matter (DM) content. Subsequent processing by peeling, washing, grating, and dewatering into granules (56% DM) caused 48% reduction in the pVAC content which was reduced to 40% after drying and milling the dried grits into YHQCF (97% DM). Preparation of recipe for bread demonstrated how the 20% composite flour dough (61% DM) contained 0.29 µg/g db pVAC representing 1.72% retention, which was later reduced to 0.25 µg/g db pVAC or 1.49% retention after baking (62%DM). On the other hand, bread loaves baked from 100% YHQCF (67% DM) retained 0.74 µg/g db pVAC representing 4.40% of the 16.83 µg/g db pVAC in the starting raw material. Conclusions: The bread produced from 100% YHQCF may contribute to the pro-vitamin A status of bread consumers in SSA more than the 20% YHQCF composite. However, both bread samples are low in pVAC. In order to attain the required retinol equivalent level after bioconversion in the human body, consumption of other foods rich in vitamin A would be required to attain the required retinol equivalent level after bioconversion in the human body but can be enhanced if consumed with other foods rich in vitamin A.Keywords: High quality cassava flour; composite flour; Bread; Pro-vitamin A carotenoid; Nutrition

Variations of β-carotene retention in a staple produced from yellow fleshed cassava roots through different drying methods

Background: New yellow cassava fresh roots biofortified with β-carotene are becoming popular in Sub-Saharan Africa in the making of traditional products for households. However, β- carotene is susceptible to rapid depletion during processing. Therefore, we investigated the impact of using different drying methods in processing these fresh Cassava roots.Methods: We processed four newly released yellow fleshed cassava varieties (01/1371, 07/593, 07/539, and 07/0220) into fermented cassava chips, flour, and corresponding dough with sun, oven, and flash drying methods respectively. The β-carotene contents were analyzed using High Performance Liquid Chromatography (HPLC). Percentage true retention (%TR) was computed.Results: The results demonstrated that %TR in chips (13.7%), flour (11.7%), and dough (5.48%) from sun drying method had the highest level of β-carotene retention compared to oven (11.3%, 7.30%, and 3.47%) and flash (8.8%, 3.33%, and 1.24%) drying methods. The duration, intensity of heat used for drying, and variety had a significant effect on β-carotene retention. Variety 07/0220 with the highest β-carotene concentration (7.81±0.13µ/g) in the fresh roots did not necessarily have the highest concentration after processing. These results suggest that β-carotene retention is not only variety specific but also depends on the method of processing. Conclusions: These results will support ongoing breeding efforts aimed at increasing pro-vitamin A content in Cassava. The information may also be significant to solutions considering fermented cassava flour and dough in Vitamin A deficient populations.Keywords: Cassava, drying, fermented flour, carotenoids, retention, Lafun

Development of an Optimized Cassava Starch-Based Custard Powder

ABSTRACTCustard powder is a dry-formulated food product primarily made from imported corn starch in developing countries. To reduce over-dependence on corn starch, this study investigated its replacement with yellow-fleshed cassava root starch (YfCRS) in custard powder formulation. Response surface methodology was used to develop an optimized cassava starch-based custard powder based on various combinations of YfCRS (90–98%) and whole egg powder (WEP) (2–10%). The result showed that the blends of YfCRS and WEP led to custard powder with wide functional, physicochemical, chemical, and micronutrient properties, with sensorily acceptable gruel. The second order response surface regression model accurately predicted most of these quality parameters (R2 > 0.90). The optimum formulation achieved to produce an acceptable custard was 90% YfCRS and 6.07% WEP. Therefore, this study showed that YfCRS might be a very good replacement for corn starch in the production of an acceptable custard.

Effect of Frying Treatments on Texture and Colour Parameters of Deep Fat Fried Yellow Fleshed Cassava Chips

Effects of frying treatments on texture (hardness) and colour parameters (L,a,b,ΔE) during deep fat frying of yellow fleshed cassava root slices (TMS 01/1371) were investigated. Slices (dimension of 40 mm × 25 mm × 3 mm) were divided into three portions and subjected to vacuum frying (fresh slices) and atmospheric frying (fresh and predried slices) and equivalent thermal driving forces (ETDF) of 60°C, 70°C, and 80°C were maintained during frying. The quality attributes investigated were best preserved in vacuum fried chips. The overall colour change in chips fried under vacuum conditions at 118°C and 8 min was the least (21.20) compared to fresh and atmospherically predried ones (16.69 and 14.81, resp.). A sharp reduction in the breaking force was obtained for all frying treatments after 8 min and this effect was the least in vacuum fried chips. First-order kinetics modeled the changes in quality attributes for all the temperatures investigated. Rate constants k (min−1) obtained for vacuum frying were almost equal to that of atmospheric frying while activation energies for hardness and colour change were 53.30 and 467.11 KJ/mol, respectively. Quality attributes studied were best preserved during vacuum frying.

Effect of Processing on the Retention of Total Carotenoid, Iron and Zinc Contents of Yellow-fleshed Cassava Roots

BACKGROUND: It was envisaged that processing of yellow-fleshed cassava roots might affect the micronutrient composition. Hence, three yellow-fleshed cassava roots were grown for 12 months in a randomized complete block design with three replications at Ibadan, Nigeria, to evaluate the effects on total carotenoid, iron, and zinc retention after processing the roots. Raw and processed storage roots were analyzed using standard methods. Percentage true nutrient retention was calculated using the concentration of each parameter adjusted for changes in weight. RESULT: There were significant genotypic differences (P<0.01) for all the evaluated characteristics. The mean total carotenoid concentration of the unprocessed storage roots was 4.90 µg/g, mean iron content was 7.47 mg/kg, and mean zinc content was 8.95 mg/kg. The concentration after processing varied depending on the product. Results indicated that boiled cassava retained the highest amount of iron and zinc, also of total carotenoid (73.5%) This was followed by gari (44.9%) and raw fufu (40.8%); cooked fufu had the lowest (21.5%). CONCLUSION: Processing cassava storage roots resulted in a significant reduction in micronutrient retention and this depended on the processing method and genotype.

Kinetics of mass transfer during deep fat frying of yellow fleshed cassava root slices

Kinetics of mass transfer [moisture content, oil uptake, total carotenoid (TC) and shrinkage] during frying of yellow fleshed cassava roots (TMS 01/1371) was investigated. Slices were divided into (i) fresh and (ii) pre-dried to 75 % moisture content before atmospheric frying and (iii) vacuum fried. Percentage TC and activation energies of vacuum, fresh and pre-dried fried samples were 76, 63 and 61 %; and 82, 469.7, 213.7 kJ/mol, respectively.

Effect of storage on the chemical composition, microbiological load, and sensory properties of cassava starch-based custard powder.

The effect of storage on the chemical, microbiological, and sensory properties of cassava starch-based custard powder (CbCP) blends as mixture of yellow-fleshed cassava root starch (YfCRS) (90–98%) and whole egg powder (WEP) (2–10%) was investigated. These were prepared using central composite rotatable design, and separately packaged in polyvinyl chloride plastic can and stored in storage box (30 ± 2°C). The chemical and microbiological analyses of the stored CbCP were evaluated at 3 weeks intervals, while the sensory property was determined at 6 weeks interval for 24 weeks. The result showed that the protein, fat, and the total-β-carotene contents of the CbCP decreased significantly (P ≤ 0.001) after storage while moisture content and microbiological load increased. All the CbCP sensory attributes were accepted at the end of storage, except taste and color. The CbCP gruel prepared from 94% YfCRS: 0.34% WEP and 90% YfCRS: 2% WEP blends were the most acceptable after storage.

Spatial distribution of dry matter in yellow fleshed cassava roots and its influence on carotenoid retention upon boiling

Understanding retention of carotenoids after different processing methods is important. This study was conducted to quantify dry matter content and carotenoids found in different sections of the cassava roots from six clones and to assess true retention of carotenoids after 30min of boiling. Retention was quantified in normalized prisms taken from proximal, central and distal sectors of the root. Dry matter content (DMC) was measured along and across the roots and varied from 14.1 to 51.0%. DMC tended to be lower at the center of the root and in distal sections. DMC affected the homogeneity of the food matrix and, therefore, contributed in spatial variation in retention of carotenoids. Average true retention (dry matter basis) was 86.6% and ranged from 76.0 and 96.7% (averages per clone and section of the root, respectively). Retention was positively associated with carotenoid content in unprocessed samples, although the relationship was weak. The study shows that during boiling weight of samples changed from slight losses to gains of up to 40% (depending on original DMC of the uncooked root), resulting in an “apparent dilution” of the carotenoids. Results suggested the occurrence of some isomerization. All-trans β-carotene losses (13%) were partially explained by increases in the 13-cis (34%) and 15-cis (8%) isoforms, as well as lixiviation (<1%) into the boiling water.