Yellow Color Research Articles

A Novel Carbon Dot-Bromothymol Blue System for Ratiometric Colorimetric-Fluorometric Sensing of Glutathione in Urine: A Smartphone-Compatible Approach.

This study presents a novel dual-modal approach for glutathione (GSH) detection using blue and yellow dual-emission carbon dots (BY-CDs) and bromothymol blue (BTB) at pH 8.0. The method employs both colorimetric and fluorometric detection modes, offering a new perspective on GSH quantification. BTB's blue coloration induces selective fluorescence quenching of the CDs' 610nm emission peak, with minimal effect on the 445nm peak. Upon GSH addition, the quinonoid structure (blue color) of BTB transforms to its benzenoid form (yellow color). This transformation triggers fluorescence restoration at 610nm and significant quenching at 445nm, enabling ratiometric fluorescence analysis (F610/F445). Concurrently, colorimetric detection is also ratiometric, based on measuring the ratio between the emerging yellow color peak (435nm) and the decreasing blue color peak (618nm) (A435/A618). The state-of-the-art aspect of this detection method lies in the strategic choice of dual-emission CDs and a dye with distinct absorption spectra that closely match the emission spectra of the CDs. This unique combination allows for dual detection with opposite responses in the two detection modes, enhancing selectivity and reliability. The probe was thoroughly characterized, and its detection mechanism was elucidated using various spectroscopic techniques. The method shows excellent linearity, a broad detection range, and low detection limits for both fluorometry (0.02 - 10.0μM, 5.88nM) and colorimetry (1.0 - 35.0μM, 301.25nM). Additionally, a smartphone-based platform was developed for colorimetric GSH determination, enhancing the method's potential for on-site analysis. The assay's practicality was validated through successful application to human urine samples, yielding excellent recovery values (97.33% to 99.13%).

Morpho–Molecular Characterization of Brazilian Common Bean Landraces Varieties and Commercial Cultivars

This study aimed to characterize forty genotypes (31 landrace varieties and nine commercial cultivars) of common bean (Phaseolus vulgaris L.) morphologically and molecularly. Morphological descriptors were evaluated during seedling, flowering, physiological maturation and post-harvest stages. Ten microsatellite markers were used for molecular screening. The markers were analyzed according to the number of alleles per locus, the allele frequency per locus and the polymorphism in content (PIC). Genetic distances and cluster analysis were performed using Bayesian inference and the UPGMA method. All black beans evaluated have anthocyanin in the cotyledons, hypocotyls and stems, and their flowers are purple. For the growth habit, 50% of the evaluated genotypes belong to type III, and at the physiological maturation stage, yellow color is predominant in the pods (85%). Through the genetic dissimilarity, three groups were observed for the likelihood reason, and five groups were observed through the UPGMA method, a strong indication of the wide genetic diversity among the evaluated genotypes. All genotypes from the Andean center of origin were grouped into the same cluster.

Impact of pasture feeding cows and seasonality on the concentration of selected fat-soluble compounds in milk and their ability to be used as biomarkers of “Grass-Fed” dairy

This study investigated the impact of proportion of pasture in the cow diet on the concentration of selected fat-soluble compounds in milk including β-carotene, lutein, retinol (vitamin A), α-tocopherol (vitamin E) and zeaxanthin. Bulk tank milk samples (n = 102) were collected weekly (n = 34) across an entire lactation from three herds operating either a high (GRS), medium (PMR) or no pasture allowance (TMR) feeding system. Concentrations of β-carotene and lutein increased significantly with increasing pasture allowance, with a > 2 fold increase of β-carotene in milk, between high and no pasture allowance treatments. GRS feeding system resulted in milks with higher concentrations of retinol, α-tocopherol, and zeaxanthin, compared to both PMR and TMR systems. A biomarker model based on the fat-soluble compounds concentration in milk, demonstrated excellent differentiation of high pasture allowance derived milks from that of medium and no pasture milks, with area under the curve scores of 0.955 and 0.971 respectively. Strong correlations were observed between the concentrations of lutein and zeaxanthin in milk and the milks corresponding yellow colour, while a significant correlation was exhibited between β-carotene and b*-value. Such correlations with milk colour may have a significant impact on the consumer preference of milk and dairy products produced therefrom.

Presentation and Management Outcome of Anterior Fontanelle Dermoid Cyst: Report of 6 Cases

Aim: The aim of study is highlight the presentation of anterior fontanelle Dermoid cyst which is relatively uncommon condition, and our ability to excised the cysts in our resource poor environment. Presentation of Cases: A retrospective study of all the consecutive patients managed by pediatric surgery unit over six years was done and the results was analysed and documented. A total of six patients was managed over a period of five years including 4 boys and 2 girls. The age ranges between 6months to five years. Discussion: Dermoid cysts are inclusion cyst mostly seen in the midline. 1, 2 They occur in head and neck or the trunk. It is a benign, slowly growing lesion, dermoid cysts are thought to arise during the third and fifth week of embryogenesis from displacement of germ cells during the formation of the neural tube. All of the patients had excision under general anesthesia. The intra operative finding is a global, cystic swelling containing amber to yellow color jelly fluid. All the patients were operated as day case surgery and were discharged home about four to six hours after recovery from anesthesia. All the patients did well postoperatively, only one patient had surgical site infection which was treated with antibiotics and wound dressing. Conclusion: Dermoid cyst is an uncommon congenital cyst mostly diagnosed during infancy, following excision recurrence is uncommon.

Studying the Influence of Salt Concentrations on Betalain and Selected Physical and Chemical Properties in the Lactic Acid Fermentation Process of Red Beetroot

This study emphasizes the significance of optimizing salt content during the fermentation of red beetroot to produce healthier and high-quality fermented products. It investigates the impact of different salt levels on fermentation, analyzing various parameters such as pH levels, dry matter content, total acidity, salt content, color changes, pigment content, and lactic acid bacteria count. This study identifies the most favorable salt concentration for bacterial growth during fermentation and storage as 2–3%. It was evaluated that salt levels fluctuated significantly during fermentation, with nearly 50% of the added salt absorbed by the beetroot tissues, mainly when lower salt concentrations were used. The fermentation process had a negative effect on the content of betalain pigments, as well as yellow pigments, including vulgaxanthin-I. It was also found that fermentation and storage affected the proportions of red pigments, with betacyanins proving to be more stable than betaxanthins, and that salt addition affected negatively pH and total acidity while causing an increase in yellow color. The pH was negatively correlated with the duration of the process, the amount of red pigment, and bacterial count. The results indicate that lower salt levels can lead to favorable physicochemical and microbiological parameters, allowing for the production of fermented red beetroot with reduced salt content without compromising quality.

Enhancing Nutritional Value of Rhubarb (Rheum rhaponticum L.) Products: The Role of Fruit and Vegetable Pomace

In this study, the quality and health-promoting components in rhubarb products sweetened with isomaltulose and enriched with fruit (apple, chokeberry, black currant) and vegetable (beetroot, carrot) pomace were assessed. All products with added pomace had higher levels of ash (27–65%) and macro- and microelements (5–436% and 5–333%) compared to the sample without pomace. The addition of pomace also increased the total antioxidant activity. The addition of pomace increased the value of gel strength (Fe) by 22–73% compared to the control; the highest value was found in the product enriched with chokeberry pomace (1.71 N). Red (a* > 0) and yellow (b* > 0) dominated all products, and values of the L* parameter ranged from 7.81 to 37.54. The brightest were the products with added carrot, apple, and beet pomace, while the darkest were those containing chokeberry and blackcurrant pomace. The values of the texture parameters decreased after storage, but to a lesser extent in the products with pomace; however, the products with pomace maintained greater antioxidant activity and retained beneficial components better than those without. A slight darkening of the products and a decrease in the proportion of red (a*) and yellow (b*) color was also observed.

Rapid and effective protocol to measure glutathione peroxidase activity

Abstract Background Intracellular hydroperoxides are harmful reactive oxygen species that can cause damage. Cells depend on antioxidant enzymes such as glutathione peroxidase (GPx) to prevent their accumulation. GPx is present in all cell compartments, including the cytosol, mitochondria, and peroxisomes. This article will present a rapid and reliable method for measuring GPx activity, which has been rigorously tested and proven dependable. Results The enzyme samples are incubated in a phosphate buffer containing specific concentrations of glutathione (GSH) and peroxide and then incubated for a suitable time to react before Ellman's reagent (5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) is added. This method, designed for ease of use, is effective and straightforward. When GSH reacts with DTNB, mixed disulfides (GS-S-DTNB) are formed, where the thiol group replaces one of the nitrobenzoic acid moieties in DTNB. The release of the nitrobenzoic acid group as nitro-2-thiobenzoate anion (TNB−) creates a yellow anion that can be measured spectrophotometrically at a wavelength of 412 nm. The intensity of the yellow color is directly proportional to the unreacted glutathione concentration. GPx activity was linked to decreased absorbance of the TNB− anion, providing a sensitive and relatively specific method for detecting thiol groups in various biological samples. Conclusion This study utilized a Box–Behnken design (BBD) to apply to response surface modeling (RSM) to determine the best conditions for measuring GPx activity. The efficiency of the new protocol was validated by comparing GPx activity in matched samples using the Bland–Altman plot analysis and reference methods. The new protocol was just as accurate as the reference one, with a correlation coefficient of 0.9991. Graphical abstract

From the few main colors to their spectra: discussion on robustness or mutability of a material’s color

The present work intends to bring a new approach to the creation of target colorations in pigment research and development. Rather than proposing a “chemist's” approach, i.e. based on knowledge of the accessible energy levels of the electrons in matter whose transfer will produce absorption phenomena, we propose here, “without any compositional guide”, to establish the spectra corresponding to the 6 main colors (red-green-blue-cyan-magenta-yellow): the created spectra for the modeling are created without trying to correspond to any specific composition. Hence, we propose herein a “process” which can be directly transferred to experimental spectra associated with experimental compounds, whatever their nature: organic or inorganic, metallic, semi-conducting or insulating, etc. Since “out of all compositional guide” does not mean “unrealistic”, we have first shown that inorganic pigments, which are made up of absorption phenomena associated with the three main types of electronic transfer, can be robustly simulated in absorbance space by a linear combination of Gaussian functions. Briefly, this work led us to show several interesting conclusions in successive stages: • A single phenomenon can already create yellow, magenta, and blue colors with very good agreement, • All colors can be created with two-Gaussian spectra, • Very different spectra can lead to the same coloration (metamerism, which is already largely documented in literature). We showed that these “metameric” spectra then have properties of robustness (maintenance of coloration) for a change of the illuminant or a shift in the energy position of the absorption bands, which can be highly variable. Some colorations are intrinsically more robust than others to these environmental changes.

Encapsulation of Curcumin With Persian Gum and Its Application to the Production of Functional Yogurt: Physicochemical, Antioxidant, Sensory Properties, and Starter Bacteria Survival Study

ABSTRACTThis study aimed to evaluate the ability of Persian gum to encapsulate curcumin and enhance its stability in dairy products. Given the increasing interest in functional foods, this study investigated the incorporation of nanocapsules containing curcumin (CLN) into functional stirred yogurt (CLN‐Y). CLN was prepared with Persian gum (PG) at different levels and included in the yogurt formula (CLN‐Y1%, CLN‐Y2%, and CLN‐Y3%). The physicochemical properties, rheological characteristics, antioxidant activity (AA), survival of starter bacteria, and sensory properties of the yogurt were evaluated over a 21‐day storage period at 4°C. The results showed that CLN significantly improved AA (reduction of 7.31% in the control vs. 1.85% in CLN‐Y3%), viscosity, hardness, and water holding capacity (reduction of 2.27% in control vs. 1.14% in CLN‐Y3%), while reducing syneresis (an increase of 10.87% in control vs. 3.16% in CLN‐Y3%) during storage (p < 0.05). CLN concentration directly affected AA. Although CLN‐Y exhibited a yellowish color and lower light intensity than the control, the samples were well accepted during storage. Increasing CLN levels led to decreased taste, color, and overall acceptance scores, and a 3% concentration is recommended for yogurt formulation due to its potential to improve yogurt quality, provide antioxidant benefits, maintain probiotic viability, and achieve high consumer acceptance during storage.

Fast, sensitive, and sustainable colorimetric detection of chlorogenic acid in artichoke waste material

Caffeoylquinic acids (CQAs) are nutraceutical polyphenols highly represented in natural sources, including artichoke waste (AW). In this study a colorimetric method for rapid and sustainable detection of a 5-CQA isomer (Chlorogenic acid) in AW extract was developed by using alkaline Tris buffer (10 mmol L−1, pH 9) to generate a yellow color associated with 5- to 3-CQA isomerization reaction, as suggested by NMR and MS analyses. The strong absorbance at 360 nm was followed by standard UV–Vis methodology. The colorimetric assay was exploited for detection of 5-CQA into leaf extract from artichoke, obtaining a value of 15.2 ± 0.3 μg/mg of dry extract, in agreement with HPLC analysis (14.3 ± 0.7 μg/mg, 106 ± 2 % recovery) used as validation technique, with excellent linear correlation and precision (R2 = 0.9996, avRSD% = 3.2 %). The method is fast and selective, offering a valuable tool for nutraceuticals identification and food waste valorization.

Inhibition of HIV-1 infection with curcumin conjugated PEG-citrate dendrimer; a new nano formulation

BackgroundNano-drug delivery systems have become a promising approach to overcoming problems such as low solubility and cellular uptake of drugs. Along with various delivery devices, dendrimers are widely used through their unique features. PEG-citrate dendrimers are biocompatible and nontoxic, with the ability to improve drug solubility. Curcumin, a naturally occurring polyphenol, has multiple beneficial properties, such as antiviral activities. However, its optimum potential has been significantly hampered due to its poor water solubility, which leads to reduced bioavailability. So, the present study attempted to address this issue and investigate its antiviral effects against HIV-1.MethodThe G2 PEG-citrate dendrimer was synthesized. Then, curcumin was conjugated to it directly. FTIR, HNMR, DLS, and LCMS characterized the structure of products. The conjugate displayed an intense yellow color. In addition, increased aqueous solubility and cell permeability of curcumin were achieved based on flow cytometry results. So, it could be a suitable vehicle for improving the therapeutic applications of curcumin. Moreover, cell toxicity was assessed using XTT method. Ultimately, the SCR HIV system provided an opportunity to evaluate the level of HIV-1 inhibition by the curcumin-dendrimer conjugate using a p24 HIV ELISA kit.ResultsThe results demonstrated a 50% up to 90% inhibition of HIV proliferation at 12 μm and 60 μm, respectively. Inhibition of HIV-1 at concentrations much lower than CC50 (300 µM) indicates a high potential of curcumin-dendrimer conjugate against this virus.ConclusionThereby, curcumin-dendrimer conjugate proves to be a promising tool to use in HIV-1 therapy.

2,4-dinitrophenol intoxication and its morphological findings as an indication of substance intake

Lethal intoxications can only very rarely be recognized during an external examination of corpses, as poisoning does not leave any characteristic findings on the deceased. The present study is a retrospective review on 2,4-dinitrophenol (2,4-DNP) intoxications in human subjects from the beginning of the 20th century until today, as well as a case report on a fatal intoxication of a 50-year old obese man in Rostock (Germany) and an introduction for toxicological analysis in post-mortem specimens of the substance ingested in these rare cases.Via selective literature search, the information on occurrence and localization of abnormal pathomorphological external and/or internal findings in cases of 2,4-DNP ingestion/ intoxication was gathered. By 2021, a total of 13 case reports with information on morphological findings due to 2,4-DNP ingestion/intoxication were found. The external findings were dominated by yellowing of the skin, followed by exanthemas/rashes and yellowing of the sclera. The internal findings included yellowing of the internal organs, yellow color of the stomach contents, yellowing of the mucous membranes and an intense yellow color of the urine. Yellowish discoloration of the skin, sclera, mucous membranes, internal organs, sweat and/or an intensive yellow discoloration of the urine are not observed in every 2,4-DNP intoxication. However, when they do occur, they are a characteristic indication of 2,4-DNP ingestion and, if localized to the skin, indicate prolonged consumption. A fatal case from Rostock in 2016 due to prolonged intake of 2,4-DNP for weight loss is exemplified. A simple, fast and cost-effective workup combined with HPLC-DAD for post-mortem toxicology ultimately delivers reliable analysis results.

Effect of trap bait and colours, and number of entry holes in monitoring of Drosophilidae (Diptera) species in a fig orchard

The study's objective was to evaluate how the capture rate of bottle traps is influenced by their color, number of entry holes, and bait liquids.For this purpose, yellow, blue, green, red, black, white, and transparent (colourless) traps were tested. Apple cider vinegar, grape vinegar, white wine, yeast, and water (control) were used as bait liquids. The number of entry holes was 2, 4, 6, and 8 holes per trap on the side of the bottle traps. The experiments were conducted in a randomised block design with three replicates in a fig orchard (variety Bursa Siyahı) with 437 trees in Aydın province from September 2018 to March 2019. In total, 48 traps were mounted on the experimental trees (1 trap per 1 tree), and counting of the drosophilid individuals in the traps was performed weekly. Red-coloured traps attracted the highest number of drosophilid individuals, followed by yellow-coloured. Regarding different baits, grape vinegar attracted the most drosophilid individuals, followed by white wine. Concerning the number of trap entry holes, the highest number of individuals were caught in traps with the highest number of holes, 8. In all traps, Zaprionus tuberculatus Malloch, 1932 was the species caught in the highest numbers, followed by Drosophila subobscura Collin, 1936 and Drosophila suzukii (Matsumura, 1931). Z. tuberculatus was mostly caught in traps containing grape vinegar, while D. subobscura and D. suzukii were mainly caught in traps containing white wine. In our study, the red-coloured traps containing vinegar with 8 entry holes were the most effective in monitoring drosophilid populations.

Structural basis of selective beta-carotene binding by a soluble protein

β-carotene (BCR) is the most abundant carotenoid, a colorant, antioxidant, and provitamin A. The extreme hydrophobicity of this hydrocarbon requires special mechanisms for distribution in aqueous media, including water-soluble carotenoproteins. However, all known carotenoproteins prefer oxygenated carotenoids and bind BCR inefficiently. Here, we present the crystal structure of the BCR-binding protein (BBP) from gregarious male locusts, which is responsible for their vivid yellow body coloration, in complex with its natural ligand, BCR. BBP forms an antiparallel tubular homodimer with α/β-wrap folded monomers, each forming a hydrophobic 47Å long, coaxial tunnel that opens outward and is occupied by one s-cisC6-C7, all-trans BCR molecule. In the BCR absence, BBP accepts a range of xanthophylls, with reduced efficiency depending on the position and number of oxygen atoms, but rejects lycopene. The structure captures a pigment complex with a Takeout 1 protein and inspires potential applications of BBP as a BCR solubilizer.

“Sunlight-Driven Catalytic Degradation of MB Dye and Multi-Cycle Re-usability Analysis of Cu2-xSe Nanoparticles”

One of the best ways to remove organic dyes based contaminant from water resources and industrial waste water is the sunlight driven photo-catalytic degradation method. The theme of the present investigation is the photocatalytic degradation of methylene blue (MB) dye using economically produced Cu2-XSe nanoparticles (NPs) catalyst under solar radiations. The Cu2-XSe NPs crystallized in the cubic structural phase with an average crystallite size around 19 nm. The direct band gap was found to be 2.1 eV. The PL spectra and corresponding CIE diagram show the Cu2-xSe NPs emitted yellow color. The SEM micrographs show that the small grains staked over others to form large grains or patches. The FTIR and EDX spectra confirmed the formation of Cu2-XSe NPs. The obtained optimum photocatalytic degradation efficiency for Cu2-XSe NPs is 90.3%. for first cycle analysis. The pseudo-first order and second order models were used to analyze the kinetic data of Cu2-XSe NPs for varying concentrations. The multiple-cycle degradation analysis by catalyst of MB dye in one day spam for continuous four cycles were also analysed and discussed. The sun light driven multi-cycle catalytic degradation confirms the re-usability of Cu2-xSe NPs for the treatment of industrial waste water and other contaminated water bodies for the survival of aquatic life and for saving environment.

Effect of inoculation with different Eurotium cristatum strains on the microbial communities and volatile organic compounds of Fu brick tea

Eurotium cristatum is the primary fungus in Fu brick tea (FBT) and plays a crucial role in its special flavor. This study investigated the effect of inoculation with different E. cristatum strains (i.e., ZJ, GX, GZ, HN, and SX) on the microbial communities and volatile organic compounds (VOCs) of FBT. A total of 113 VOCs were identified in all samples, with the concentration of VOCs (alcohols, aldehydes, and ketones) significantly higher in GXE FBT than in other samples. The core VOCs of GXE (19), GZE (16), HNE (19), SXE (15), and ZJE (13) FBT were identified using orthogonal partial least squares discriminant analysis and relative odor activity value (ROAV) analysis. Methional (a27), butanal (a41), 1-octen-3-one (a69), and ethyl acetate (a77) were key markers for inoculated FBTs, and 1-octen-3-ol, dimethyl disulfide, and acetoin-M were the specific markers of HNE. Linalool and (E)-2-octenal were particularly prominent in GXE, and isoamyl acetate-D was an important aroma component of GZE. Differences in microbial diversity were observed among the different inoculated fermented FBTs, and E. cristatum inoculation remarkably influenced the richness and diversity of bacterial communities. The VOCs were closely associated with fungi and bacteria, and 19 potentially dominant microorganisms (10 fungal and 9 bacterial genera) correlated with VOCs were identified. Among them, Aspergillus (fungi) and Pseudomonas (bacteria) exerted the greatest role. The FBT inoculated with E. cristatum from ZJ had the highest content of theaflavins and theabrownins, which intensified the red and yellow colors of the tea. E. cristatum greatly decreased the free amino acids and fatty acids, contributing to the aroma formation of FBT. Therefore, inoculating FBT with E. cristatum remarkably influenced the microbial communities and improved its flavor profile. This work provides a theoretical foundation on the role of E. cristatum in the formation and regulation of FBT flavor.

Colourimetric changes experienced in three types of aligners according to the L'Eclairage Commission.

The objective was to compare the colourimetric increment (L*, a*, and b*) of three types of aligners after subjecting them to two stains and to evaluate the initial colourimetric characteristics. A total of 120 aligners (40 Invisalign®, 40 Spark®, and 40 QuickSmile®) were immersed in two different agents (distilled water and coffee). Measurements were taken using a spectrophotometer before immersion (T0), after 12 hours (T1), and after 7 days (T2). Colour changes (ΔE*) were evaluated based on the International Commission of L'Eclairage, and subsequently converted to National Bureau of Standards units. The measurement was repeated three times. The intraclass correlation coefficient, one-way ANOVA, Tukey's post hoc test, and the independent t test were used (P ≤ .05). At T0, significant differences were observed for a*: Invisalign® and Spark® tended towards redder tones, whereas Quicksmile® leaned towards greener shades. Regarding b*, all aligners tended towards yellow, with Invisalign® exhibiting the least tendency. No differences were found in water between T1 and T0. In the intervals, T2-T1 and T2-T0, Spark® showed more noticeable changes, whereas for Invisalign® and Quicksmile®, the change was only slight. In coffee, a shift to another colour was detected in T1-T0, T2-T1, and T2-T0 for Invisalign®, whereas for other brands, it was barely noticeable except for T2-T0 in Spark®, where the change was appreciable. In coffee, Invisalign® tended towards darker values (L*), turning redder (a*), and more yellow (b*) in T2-T1 and T2-T0. Invisalign® aligners initially presented a more reddish and less yellowish colour. In water, after 7 days, a noticeable colour change was found for Spark®. In coffee, a greater loss in brightness and a change to red and yellow were observed after 12 hours and 7 days for Invisalign®. This study highlights the importance of considering the impact of coffee on the colouring of aligners, especially with Invisalign®, which shows more noticeable changes.

Dry-Cured Sausages “Salchichón” Manufactured with a Valorized Ingredient from Red Grape Pomace (Var. Tempranillo)

The inclusion of an ingredient made from red grape pomace (RGP) var. Tempranillo was evaluated for the preservation of a traditional dry-cured sausages (salchichón). The pomace was valorized through thermal blanching (103 °C for 1 min) and hydrostatic high-pressure treatment (600 MPa/5 min) before the addition to salchichón. Four formulations of salchichón were evaluated, including a negative control (NC—without red grape pomace or synthetic additives), positive control (PC—with ascorbic acid and nitrites), low level (LL—0.5%), and high level (HL—1%) of RGP. Physicochemical, microbiological, and sensorial effects were analyzed. RGP reduced the final pH of salchichón and favored the growth of lactic acid bacteria at similar levels as PC. The addition of ascorbic acid and nitrites resulted in a final product with a redder and less yellow color than the other formulations. This cured color was not reached with the addition of RGP. However, its inclusion slightly reduced lipid and protein oxidation in salchichón. PC showed high levels of sulfur and terpene levels in a volatile profile, although at a sensory level, only differences in spicy taste were not noticed by panelists. The incorporation of the ingredient could enable the substitution of nitrites with valorized red grape pomace in sausages, although the desirable color achieved with nitrifying salts was not fully attained.

Civic Cultural Literacy Research Trend in Primary Schools

This study uses VOSviewer bibliometric analysis to chart the upward trajectory of civic cultural literacy research, particularly in primary schools. The goal of this study was to examine civic cultural literacy research trends using bibliometric analysis. Article data were collected using publication reference management systems and the Publish or Perish (PoP 8) database. Direct the search process by including the keywords "Cultural Literacy, Civic Literacy, Civic Cultural Literacy, and Elementary School" in the article's title and abstract. 1000. articles are utilized as samples and deemed appropriate. The literature included in the Google Scholar index between 2014 and 2024 provides research material. The statistics reveal that over time, the citations related to this paper are very few and even declined considerably. The statistics reveal that the number of citations linked with this work has decreased dramatically over time, as seen by the fact that there were 9004 citations in 2014. However, there will be just 410 citations at the end of 2023, and 28 citations by 2024. Based on density visualization data, the yellow color in the visualization suggests a dark direction, implying that this research is still extremely poorly done and provides a huge chance for future research on the phrase above

ANALISIS SIFAT FISIKA DAN SIFAT KIMIA GEL BIODIESEL DARI MINYAK BIJI PALEM PUTRI (Adonidia merrillii)

Biodiesel is an alkyl ester of long-chain fatty acids, the most common of which are methyl esters or ethyl esters. The manufacturing process involves esterification and transesterification. Traditional sources of biodiesel raw materials such as palm oil, soybeans, and jatropha have been widely studied. The princess palm, widely known as an ornamental plant, produces fruit containing seeds with quite high oil potential. This research aims to produce biodiesel and determine the physical and chemical properties from putri palm seeds. This research is experimental research with a quantitative descriptive approach. Putri palm seed oil is extracted using the soxhletation method with n-hexane solvent. The oil was then esterified using methanol with a catalyst of 1% sulfuric acid with a mole ratio of oil and methanol of 1:8 at a temperature of 60°C for 3 hours. The esterified oil is then transesterified using methanol and KOH with a methanol to oil ratio of 1:20 with KOH of 0.8% for 2 hours at a temperature of 60°C. This research has succeeded in making biodiesel from putri palm seeds. The biodiesel produced is in the form of a gel referred to as biodiesel gel with a yellowish brown color with a density of 1,236 gr/ml and a pour point of 36-38°C. The FFA/ALB value is 0.587%, the acid number is 1.177 mg KOH/g, the IOD number is 34.514 (g-I2/100 g), the saponification number is 63.19 mg KOH/g, the heating value is 48.088 MJ/Kg, and the cetane number 38,620. The IOD number, saponification number, and heating value meet the SNI standards for liquid biodiesel in general, while the cetane number and ALB value are close to SNI standards.