Year-long Survey Research Articles

Impact of urban landscape patterns on butterfly diversity in Fuzhou City parks

Butterflies are crucial bioindicators of urban environmental changes. Utilizing urban green spaces to create ecological corridors and habitats is essential for maintaining biodiversity. Therefore, understanding the factors that influence landscape patterns in urban green spaces and their impact on butterfly diversity is crucial. This study investigated how landscape patterns in urban green spaces affect butterfly diversity through a year-long survey across 24 urban parks in Fuzhou. The main findings were as follows: (1) A total of 7786 butterflies from 59 species were recorded, with the highest species diversity and richness observed in Fushan Country Park. Seasonal abundance peaked in summer, while richness was highest in autumn. Species composition varied significantly across park types. (2) Monophagous butterfly diversity was positively correlated with woodland proportion and negatively to waterbody largest patch index. Polyphagous and oligophagous butterfly diversity showed positive correlations with woodland aggregation index and grassland proportion within 500 m buffers, but negative with construction land proportion. Factors such as the waterbody proportion and landscape division index influenced butterfly diversity in different activity spaces. (3) Butterfly communities with different feeding types and vertical activity space ranges responded differently to landscape pattern indices. Seasonal variations indicated that polyphagous butterflies and those occupying the middle activity space exhibited similar responses to environmental factors.

Effects of environmental factors on host-parasite interaction patterns in backyard-tethered goats of Kerala, India

The environment is the most important stratum in the epidemiological triad of a parasitic disease and any variations in the environmental factors may decide the dynamic occurrence and existence of different lifecycle stages of these parasites. The present study investigated the correlations between key biometeorological and demographical parameters with the incidence of different gastrointestinal parasites and hemoparasites among goats. Four hundred and thirty-two goats were screened for parasitic infection in a yearlong survey conducted from July 2022 to June 2023 in the Teaching Veterinary Clinical Complex, Mannuthy, Kerala, India. The weather parameters (Tmax, Tmin, RH, THI, and bright sunshine hours), air quality parameters [AQI, PM2.5, and PM10], and demographic parameters (gender and age) were recorded along with the test positivity of different categories of gastrointestinal parasites and hemoparasites in goats by routine fecal sample examination and blood smear examination, respectively. The infection level was ranked based on the severity of the infection. The mean and daily variations in biometeorological parameters were calculated and the data were statistically analyzed to figure out the pertinent correlations in host-parasite-environment interaction patterns. High levels of parasitic infections with significant month-wise variations and climate-correlated peak infection patterns were noticed. The incidence of parasites was negatively correlated to all parameters except humidity, indicating more severe parasitism during monsoon months. The significant variations in the host-parasite interaction dynamics point towards the need for detailed explorations concerning the lifecycle of each specific parasite with a focus on the possible environment-favourable and resistant lifecycle stages. Future studies may be designed from a biometeorological perspective to develop a crucial understanding of host-parasite-environment interactions in goats ensuring sustainable goat farming.

Seasonal Dynamics and Avian Diversity along the Adyar Riverbank: Insights from a Year-Long Survey

The avian survey conducted along the Adyar Riverbank (Manapakkam) from January 2023 to December 2023 documented a total of 3120 individual birds across 75 species from 16 avian orders, highlighting the significant biodiversity within this urban ecosystem. This study aimed to assess seasonal variations in bird diversity and abundance, emphasizing the river’s role as an important habitat. The highest diversity was observed during winter, specifically in February 2023, with a diversity index of 296.80 and a Simpson’s index of 0.22. A total of 36 species and 636 individuals were recorded. In contrast, the lowest diversity was observed in summer, particularly in July 2023, with a diversity index of 29.29 and a Simpson’s index of 0.05, corresponding to 18 species and 114 individuals. The monsoon and post-monsoon periods exhibited a prominent increase in diversity due to the arrival of migratory species and improved habitat conditions. Important species recorded throughout the year included the Paddy Field Pipit, House Crow, and Rufous Treepie, while conservation-significant species such as the Black-headed Ibis and Spot-billed Pelican were also observed. Thus, the present study underscores the Adyar River’s ecological importance and highlights the need for continuous monitoring and targeted conservation efforts to maintain avian biodiversity in urban river ecosystems.

Investigation of thermal comfort and preferred temperatures among rural elderly in Weihai, China: Considering metabolic rate effects

As the population ages, the thermal environment indoors for the elderly has garnered extensive attention. However, current research on thermal comfort for the elderly is not comprehensive, particularly for rural elderly individuals who experience unique thermal perceptions and adaptation behaviors due to physical changes. Moreover, metabolic rates, which are often overlooked in thermal comfort studies for the elderly, play a crucial role. A year-long survey of thermal comfort was conducted in the rural areas of Weihai, a coastal city in China's cold region, collecting 203 questionnaires. Three different values of metabolic rates (recommended values, estimated values, and values calculated using Basal Metabolic Rate and Physical Activity Ratio) were analyzed. The results demonstrate that the Basal Metabolic Rate and Physical Activity Ratio method provides predictions of thermal sensation that more closely align with actual thermal sensations. The adaptive coefficient of the aPMV model was found to be −0.38 in colder environments and 0.272 in warmer ones. Additionally, using PMV regression, TSV regression, and Griffiths' method, we calculated the local elderly's comfort temperatures to be 18.9 °C (15.43–22.57 °C), 19.3 °C (15.16–22.75 °C), and 21.63 °C (21.34–23.49 °C) respectively. This study introduces a straightforward method for calculating metabolic rates in elderly individuals, offering new insights for research on thermal comfort in the elderly and expanding the database for thermal comfort studies. It lays a foundation for future design and standardization of housing for rural elderly populations.

Acoustic study of urban bat diversity in Veliko Tarnovo, Bulgaria

Urbanisation presents both challenges and opportunities for bat populations. Understanding the composition and distribution of urban bat species is the first step toward their effective conservation. However, comprehensive studies on urban bat diversity remain scarce in Bulgaria. To address this gap, we conducted a year-long acoustic survey on five different habitats in the urbanised territory of Veliko Tarnovo. Our study identified the presence of eight bat species and other distinct acoustic groups within the urban environment. Bats were found to utilise various habitats across the urban landscape. Although the activity was highest during May and September, bats remained active all year round, including the winter. Our study provides insights into synanthropic bats’ seasonal and microhabitat-dependent use of urban areas. These findings will establish a baseline for future research focusing on habitat preferences, population dynamics, and interactions with urbanisation. Such research is essential for the effective management and conservation of bat populations in urban environments.

Riverine concentrations and export of dissolved silicon, and potential controls on nutrient stoichiometry, across the land–ocean continuum in Great Britain

Silicon (Si) is an essential nutrient element in freshwater and marine ecosystems, and its abundance relative to macro-nutrients (N, P) can impact phytoplankton communities in eutrophic rivers and estuaries. This study is the first national assessment examining (i) the primary sources (geological, biological, landcover) and controls (geomorphological, precipitation) on the transport of terrestrial dissolved silicon across Great Britain to the ocean, and (ii) the current extent and nature of its interactions with macro-nutrients in these catchments in relation to its potential impacts on phytoplankton community structure. It uses results from a year-long survey of 41 rivers along with historical data. Highest concentrations of dissolved Si (4–5.5 mg L-1) were found in rivers of the chalk- and sedimentary sandstone-based catchments of southern Great Britain and the hard sandstone catchments of Scotland. Catchment yield rates for dissolved Si varied between 0.2 and 2.6 t km−2 yr−1, with highest yields found in catchments with higher precipitation and runoff. Analysis of river N:P and dissolved Si:N ratios suggested that the sampled rivers were typically N enriched, and P limited with respect to dissolved Si. Molar dissolved Si:N ratios < 1, an indicator of river eutrophication, were associated with total nitrogen concentrations exceeding 1.8 mg L-1 or greater. The Indicator of Coastal Eutrophication index was used to assess the potential role of dissolved Si in the eutrophication of coastal waters. Negative values indicating limited eutrophication potential to non-siliceous algae were generally found, although some rivers had annual Indicator of Coastal Eutrophication index values exceeding 0, with values as high as 35 kg C km−2 day−1. In many eutrophic rivers, high dissolved Si concentrations derived from catchment lithology, kept the Indicator of Coastal Eutrophication index values below zero. Results have demonstrated that high N and P export have likely shifted most Great Britain rivers and coastal waters beyond the stoichiometric range where diatoms dominate production and into one where non-siliceous algae maybe increasingly present. Thus, future assessments of macro-nutrient management schemes, such as those involving wetlands should include dissolved Si routinely due to its stoichiometric importance.

Spatio-Temporal Influence on the Distribution of Forensically Relevant Blowflies (Diptera: Calliphoridae) in Gyeongsangnam-do, South Korea.

The study of blowfly (Diptera: Calliphoridae) biodiversity and distribution is crucial for forensic investigations. Abiotic and biotic factors, such as season and habitat type, have a significant impact on blowfly populations. However, only a few forensic entomology studies have been conducted in South Korea, particularly in the Gyeongsangnam-do region. To address this, an extensive year-long survey was conducted to analyze the compositions, habitat preferences, distribution, and seasonal abundance of forensically relevant blowflies in urban and forested habitats of Gyeongsangnam-do, with sampling conducted twice a month using mouse carcass-baited traps set for 48 h each time. A total of 3470 adult blowflies were recorded, encompassing five genera and 13 species, with a noted absence of specimens during the winter months. The predominant species was Lucilia porphyrina, accounting for 37.2% of the total sample, followed by Chrysomya pinguis (27.6%), Lucilia sericata (7.6%), and Lucilia illustris (7.1%). The species composition was consistent across all surveyed regions; however, seasonal variation in species diversity was evident, with a peak in spring and a decline in summer. Notably, certain species exhibited clear preferences for either urban (Calliphora calliphoroides and L. sericata) or forested habitats (L. porphyrina and Ch. pinguis). This pioneering study elucidates the diverse blowfly communities in Gyeongsangnam-do, highlighting significant seasonal and habitat-dependent variations. These findings enrich our understanding of blowfly ecology in this region, offering valuable insights for forensic applications and underscoring the necessity for ongoing entomological surveillance and research.

Effects of Seasonality on the Large and Medium-Sized Mammal Community in Mountain Dry Forests

Seasonality drives natural processes, impacting environmental factors like temperature and resource availability, leading to shifts in wildlife communities. The Andean dry forests exhibit a marked seasonality, with a dry and cold season (May–September) and a warm, wet season (October–April). In a year-long remote camera survey in Southern Bolivia, we identified 29 medium to large mammal species, 18 outside their known distribution ranges. While overall species richness remained stable, photographic records varied between seasons. Capture rates, reflecting species richness and abundance, were more influenced by season and habitat. Wet season rates were lower, but higher in all other habitats compared to the mountain bush and grasslands. Rates increased with altitude and distance to hiking trails, but decreased with increasing distance from main roads. Medium to large mammals were more active during the dry season, indicating adjustments in response to seasonal changes. Our results suggest a cumulative impact of various factors beyond mere seasonality, and call for adjustments in global species distributions. Moreover, emphasize the need for biodiversity monitoring in dry forest habitats, particularly regarding responses to environmental shifts and human-induced alterations.

Diversity and status of butterfly fauna at Kurukshetra University campus, Haryana, India

Campuses of educational institutions in India serve as important reservoirs for different faunal components, including ecologically sensitive species like butterflies. To record the diversity, status, and occurrence of butterfly fauna in Kurukshetra University Campus, Haryana, a year-long survey was done from July 2021 to June 2022. A total of 710 individuals of butterflies belonging to 39 species, 32 genera, and five families were recorded. Nymphalidae represented the highest diversity with 13 species followed by Lycaenidae (11 species), Pieridae (10 species), Papilionidae (3 species), and Hesperiidae (2 species). The number of individuals encountered was maximum in family Pieridae (n = 158) while the minimum was in family Hesperiidae (n = 4). Species richness, abundance, and diversity differed significantly (P &lt;0.05) across the different seasons. Species richness was recorded to be the highest in summer season (35 species) followed by monsoon, post monsoon, and winter. Among the recorded species, one species, i.e., Common Baron Euthelia aconthea is protected under Schedule II of the Wildlife (Protection) Amendment Act (2022). The findings of the present study support the importance of the higher educational institution campuses in providing valuable habitat and resources for butterflies.

Why do Chinese multinationals in South Africa get benefits from digital business strategy?

Background: Chinese multinational enterprises (MNEs) are obsessed with implementing digital business strategy in global competition, but there is limited knowledge about how and when the MNEs can achieve performance.Aim: This study aims to clarify the influence of digital business strategy on MNEs’ performance in South Africa, and whether this impact is mediated by exploratory and exploitative learning and moderated by organisational memory level and dispersion.Setting: Senior executives from the MNEs participated in the year-long survey. Before the survey, the participants were communicated and agreed, and the survey was completed by email.Method: Two-stage data from 314 MNEs in South Africa were obtained. Hierarchical regression analysis and Hayes Process Macros were used.Results: The results show that digital business strategy positively influenced performance, and the relationship was mediated by exploratory and exploitative learning. Organisational memory level and dispersion had an inverted U-shaped moderating effect on the relationship between digital business strategy and exploratory and exploitative learning.Conclusion: This study provides the first insight into the relationship between digital business strategy and MNEs’ performance in South Africa. It reveals the mediating mechanism and boundary conditions of this relationship, making an important contribution to the literature concerning digital business strategy.Contribution: This study encourages MNEs in South Africa to implement digital business strategies according to local conditions. What’s more, exploratory and exploitative learning is a strategic process that cannot be ignored, and moderate organisational memory can help these MNEs benefit from digital business strategy better.

Impact of Energy-Consuming Air Conditioning Systems on People's Thermal Comfort and Preferences: Comparative Study of Iraq and Gulf Cooperation Council Countries

This study investigates the impact of the intense usage of air-conditioning systems on the thermal comfort requirements of building occupants. It compares the thermal comfort requirements of building occupants in Iraq and Gulf Cooperation Council (GCC) countries. Iraqis have limited usage of air-conditioning units, whilst people in GCC countries intensely operate these systems. Research work underpinning this article involved undertaking a year-long thermal comfort survey in Iraq, and an intensive analysis of thermal comfort studies conducted in GCC countries. Results show that, in Iraq, people experience a 2–50 °C annual indoor temperature range, and their annual comfort range is 14–35 °C. In GCC countries, due to the intense usage of air-conditioning systems, the widest recorded annual indoor temperature range is 17.2–31.0 °C, and the widest annual comfort range is 20.0–27.8 °C. These results demonstrate the significant impact of air-conditioning systems on narrowing the thermal comfort limits of building occupants leading to high energy consumption. This study presents a novel comparison between two cases highlighting the impact of air-conditioning systems on the thermal comfort requirements of building occupants. The results of this study can be used to inform the development of thermal comfort standards that better consider people’s adaptation capabilities to help reduce energy consumption for heating and cooling purposes.

Uncovering a Severe Patient Group With Pollen-Related Extrarespiratory Allergic Symptoms: A Year-Long Diary Survey in Japan

BackgroundThe most common symptoms of pollen allergy are rhinitis and conjunctivitis. However, in real-world clinical practice, we sometimes encounter patients with pollen allergy suffering from severe extrarespiratory symptoms including skin, gastrointestinal, or flu-like symptoms in relation to exposure to sensitized pollen. ObjectiveThe aim of this study was to elucidate the extrarespiratory symptoms in patients with pollen allergy MethodsWe performed a non-drug-focused, prospective study of patients with pollen allergy (n=384). During the 1-year observational period, they were asked to complete a weekly e-diary consisting of visual analogue scale (VAS) scores to assess all symptoms experienced in various organs over the past week. An association between seasonal pollen levels and seasonal increase in VAS scores was evaluated using a mixed-effect model for repeated measures. A k-means cluster analysis was performed to identify a group of patients experiencing stronger extrarespiratory symptoms. ResultsIn patients sensitized to grass or birch pollen, higher seasonal levels of these pollen grains were associated with higher VAS scores for headache, gastrointestinal symptoms, skin symptoms, and fatigue. A cluster analysis identified a group of severe pollen-allergic patients with higher extrarespiratory symptoms (n=42). This group was characterized by a higher frequency of comorbid food allergy/atopic dermatitis, higher rate of IgE-sensitization to pollens, and higher impaired activity and work productivity. ConclusionThis 1-year survey identified a small but non-negligible group of patients with pollen-related extrarespiratory symptoms. More attention should be paid to this patient group considering their impaired activity and work productivity.

Impacts of extreme weather events on microplastic distribution in coastal environments

Microplastic pollution is a pressing environmental threat to the ecosystem, which can be influenced by varying weather factors. With arising weather extremes in recent years, it is crucial to assess the weather effects on coastal microplastic pollution. In this study, we conducted a year-long baseline survey on beach sediment and surface seawater in Hong Kong, and additional surveys after rainstorm and typhoon events. Our data reveals that microplastic abundance was 5 times higher in wet season over dry season. Yet, the seasonal variation was insignificant when considering only the baseline condition, suggesting the role of extreme weather as the main driver of the seasonal variation in microplastic distribution. Typhoons and rainstorms induced 5.1 to 36.4 times and 1.9 to 11.7 times more microplastics in beach sediment, respectively, and 3.5 to 6.0 times and 2.5 to 4.3 times more microplastics in surface seawater, respectively. The larger microplastic mass in beach sediment and the increased proportion of hard fragments under extreme weather conditions indicate the larger mobility of heavier plastic debris from a wider source. We identified positive correlations between plastic levels and multiple weather factors (including rainfall, wind and tide), suggesting the potential terrestrial inputs of microplastics via surface runoff and wind transport, and the potential redistribution of microplastics from deep to surface sediment via wave agitation. We also identified a strongly positive correlation between macro- and microplastic abundance in beach sediment, suggesting the potential plastic fragmentation under strong wave abrasion, which may intensify the coastal microplastic pollution. This study sheds light on the need for reinforced security of waste management systems to avoid terrestrial plastic inputs under extreme weather forces, as well as the timing of coastal cleanup work in terms of limiting plastic fragmentation and achieving better cleanup efficiency.

Dietary patterns and environmental impacts of Chongqing hotpot in China

Understanding the food choices of local people is key to identifying practical opportunities for sustainable dietary change. Here, we assess the dietary pattern and environmental impacts of Chongqing hotpot in China, a local food in the Szechuan Basin enjoyed by ∼110 million people primarily in restaurants with an increasing popularity nationwide. Through a yearlong survey, we find that hotpot meals are characterized by overconsumption (588.7 g/cap/meal), especially of animal-based products (∼70%). The embedded carbon footprint (CF) of hotpot reaches 3.5 kg CO2e per capita per meal; by contrast, the CF of recommended diets per capita per day in China is 2.3–3.7 kg CO2e. Its water footprint shows a similar trend. Hotpot meals are also more wasteful than other types of restaurant food (80.4 vs. 54.1–75.0 g/cap/meal). Our study raises concern about the environmental impacts of Chongqing hotpot. To mitigate these impacts, intervention strategies that should be considered include small-portion plates and plant-based meat alternatives.

Rediscovery of the lost skink Proscelotes aenea and implications for conservation

Biodiversity loss is recognized as a grand challenge of the twenty-first century but ascertaining when a species is “lost” can be incredibly difficult—since the absence of evidence is not evidence of absence. This may be a relatively easy task for large and conspicuous animals, but extremely difficult for those living hidden lives or at low population sizes. We showcase this challenge by focusing on Africa’s montane skink, Proscelotes aenea (Barbour & Loveridge 1928). In this study, we embarked on a year-long intensive survey to find this fossorial species in Lumbo, Northern Mozambique, the only remaining location where it may still occur but was recorded for the last time over 100 years ago. We located the species already after 20 days of intensive and targeted searching by five members of our team. The finding allowed us to describe, for the first time, details on the biology and ecology of the species, alongside photos and videos of live specimens (including a pregnant female), and to sequence DNA from the species, which we used to infer the phylogenetic placement. Our combined 12S and 16S phylogenetic analysis weakly suggest that the genus Proscelotes may not be monophyletic and therefore requires further phylogenetic work and potentially taxonomic revision. We also gathered evidence of a possible decrease in population abundance and, based on the species' ecology, we identified urbanization as a potential key threat, which could lead to the local or global extirpation of the species. We call for urgent conservation actions that help protect the future of the montane skink, and additional surveys to map its full distribution. As countries now work towards implementing the goals and targets of the Kunming-Montreal Global Biodiversity Framework, our study demonstrates the need for proper investments in biodiversity inventories and monitoring in order to halt species extinctions by 2030.

A quantitative evaluation model of outdoor dynamic thermal comfort and adaptation: A year-long longitudinal field study

The understanding of human outdoor thermal comfort demand and thermal adaptation contributes to sustainable urban design as well as city resilience in the context of human health and wellbeing. Humans' past thermal experience influence their outdoor thermal comfort. However, the quantitative relationship between the past thermal experience and outdoor thermal comfort is still not clear. This study aims to reveal quantitative relations of the impact of people's past thermal experience on adaptive thermal comfort and to develop a new outdoor adaptive thermal comfort model. A year-long longitudinal questionnaire survey along with a combination of outdoor thermal environment campaigns was carried out in Chongqing, China. It began on August 15, 2020, and finished on August 19, 2021. Through the analysis of 2240 valid responses to the questionnaire survey, the outdoor thermal adaptation characteristic and dynamic thermal comfort evaluation of the respondents were revealed. The results show that the quantified temperature of past outdoor thermal experience is the quadratic correlation with the thermal sensitivity coefficient and deviation constant, and the linear correlated with outdoor thermal demands. Based on the quantitative analysis, a new outdoor adaptive thermal comfort model has been developed as a function of the exponentially weighted sum of historical mean air temperature series (MeanTrm). The outdoor adaptive thermal comfort zones by 80% and 90% satisfactions thereby have been first drawn based on the Universal Thermal Climate Index (UTCI). The study developed a methodology for the evaluation of dynamic outdoor thermal comfort which can be used for different climate regions.

The difference in thermal comfort between southern and northern Chinese living in the Xi'an cold climate region.

A year-long longitudinal survey regarding perceptions of outdoor thermal conditions and thermal comfort was conducted in Xi'an, a City in a Chinese cold region. The survey included micrometeorological measurements and a longitudinal questionnaire. The thermal comfort and adaptability of southern Chinese (people from Changsha and Guangzhou) and northern Chinese (people from Xi'an) in Xi'an were studied from the three aspects of psychological, physiological, and behavioral differences. The results of similar studies in other regions were compared with those of this study. Regarding psychological differences, northerners were more adapted to Xi'an's climate than southerners, with an expected temperatures of 20.7℃ and 24.1℃ for northerners and for southerners, respectively. Regarding physiological differences, the neutral temperature of the northern population was 22.12℃, while that of the southern population was 21.12℃. The neutral temperature for the southern population in Xi'an is similar to that of northern people living in Xi'an. Regarding behavioral differences, northerners were more likely than southerners to maintain their thermal comfort by adjusting their clothing when they experienced a change in the outdoor environment. This study not only indicates that there were differences regarding the thermal comfort of people originating different regions but also provided support for fully explaining the mechanism of climate adaptation of human thermal comfort. In addition, this work provides basic data regarding formulating outdoor thermal comfort standards and provided data support for personalized thermal comfort.

Art and craft material use patterns by pre-school and elementary school children at home and school: a year long survey for refining exposure assessments.

Use frequency and times are critical parameters for estimating realistic chemical exposures associated with the use of consumer products. Very limited information is available in the published literature for children's use patterns of art and craft materials at home and school. Conduct a year-long survey of art materials use at home and school by pre-school and elementary school children, teachers, and parents which can be used to refine chemical exposure assessments for these consumer products. Parent and teacher online surveys were conducted on the daily use of markers and monthly use of fifteen additional art and craft materials. Daily marker use by elementary children was widespread at home and school (65% and 80%, respectively). On average, pre-school and elementary students used markers for 27 min per day, more than double daily home use. Adults used markers for longer durations relative to their children/students with teachers reporting the highest average daily usage time. School use of general art materials exceeded home use for both age groups, with elementary children using art materials more frequently than their pre-school counterparts. Examples of how these data can be used to refine exposure estimates are provided. Accurate art material usage data contributes to refined estimates of chemical exposure for these consumer products. A year-long online survey was conducted which measured daily frequency and duration use for markers and comparable monthly use of other art materials for pre-school and elementary school children, their parents and teachers. Such use information is critical for estimating chemical exposures associated with this class of consumer products.

Answers blowing in the wind: Detection of birds, mammals, and amphibians with airborne environmental DNA in a natural environment over a yearlong survey

AbstractAnalysis of environmental DNA (eDNA) from passively collected airborne dust has demonstrated broad success for sensitive and robust detection of plants. Recent experiments at small spatial scales have suggested that animals can also be detected using airborne eDNA. However, airborne eDNA analysis has never been used for a long‐term whole‐community assessment of a natural terrestrial community or with passive dust collectors. We conducted a metabarcoding survey targeting vertebrate eDNA from dust carried in the air on an approximately 130‐acre shortgrass prairie passively collected over the course of a year. Our survey detected a wide variety of animal forms including an amphibian species, several bird species, and both small and large mammals. We found that airborne eDNA signals changed with known patterns of animal activity, wind speed, and rainfall. Overall, we demonstrate that passively collected airborne dust carries eDNA from terrestrial animals and could be used to detect a wide variety of terrestrial vertebrate species in a natural environment with minimal effort. To develop this as a valuable monitoring tool, research needs to focus on the ecology of eDNA carried in the air, which includes the origin, state, transport, dispersal, and fate of eDNA in the environment.

Monitoring threatened species with environmental DNA and open ecological data: Local distribution and habitat preferences of scalloped hammerhead sharks (Sphyrna lewini)

Evidence-based environmental management is urgently required to enable progress towards global conservation commitments. To fill large-scale biodiversity knowledge gaps, governments, organisations and communities must adopt innovative ecological monitoring techniques. The emergence of environmental DNA (eDNA) survey methods, as well as the increased availability of in situ ocean observation and satellite remote sensing data, offers opportunities to overcome significant logistic and financial challenges previously associated with traditional biodiversity monitoring. Here, a 1.5 year-long eDNA survey reveals the spatial and temporal presence of ‘critically endangered’ scalloped hammerhead sharks (Sphyrna lewini) in Apra Harbor, Guam. The survey confirms the presence of S. lewini in the region, and shows eDNA detections are most frequent at the beginning of the dry season, in January. Combined analysis of eDNA results with open (freely available) ecological data identifies that S. lewini presence is associated with increases in water velocity, turbidity and wind direction, as well as decreases in temperature and wind speed. Additionally, the data indicate S. lewini are most frequent at sampling sites characterised by high turbidity, shallow depths, and large distances from the harbor entrance. Information on local distribution and habitat preferences generated here is fundamental to the establishment of management protocols that aim to minimise negative anthropogenic impacts on S. lewini in Apra Harbor. The work provides an example of contemporary marine monitoring for the efficient generation of biodiversity data; an approach that will enable informed management decisions and help to reduce the risk and rate of species extinctions throughout the next decade.