Metabolic engineering of carotenoid biosynthesis in Yarrowia lipolytica.

RNA helicase CgDBP4 improves tolerance and fermentation performance of Candida glycerinogenes and Yarrowia lipolytica from undetoxified cellulose hydrolysate

Lipid accumulation in nitrogen and phosphorus-limited yeast is caused by less growth-related dilution.

Staying productive under pressure: systems evaluations of β-carotene production in Yarrowia lipolytica under continuous fermentation.

Metabolic model-guided strain design for improved succinic acid production in Yarrowia lipolytica.

The tropical marine yeast Yarrowia lipolytica NCIM 3589 as a feed for Artemia salina: evaluation of nutritional and immunostimulatory properties

2'-Fucosyllactose (2'-FL), the most abundant oligosaccharide in human milk, exhibits diverse bioactivities and is added to infant formula to simulate breastfeeding. Herein, Yarrowia lipolytica was employed as a microbial chassis for the de novo biosynthesis of 2'-FL. A complete biosynthetic pathway was established via genomic integration, resulting in an initial titer of 2.16 g/L. Systematic metabolic analysis identified the fucosyltransferase (FutC)-catalyzed reaction as the key rate-limiting step, alleviated by promoter engineering upregulating FutC, enhancing 2'-FL production. Additional strategies, including enhancing GDP-fucose supply, knocking out the PFK gene to redirect carbon flux, and strengthening the pentose phosphate pathway (PPP), further boosted production. Fed-batch fermentation with a mixed carbon source led to a titer of 105.88 g/L and a productivity of 0.98 g/L/h in strain YL16-6, representing the highest reported 2'-FL production in a plasmid- and antibiotic-free microbial system to date. This work demonstrates the potential of Y. lipolytica as a robust, scalable cell factory for the sustainable and antibiotic-free industrial production of 2'-FL.

A workflow to explore elongase diversity and extend the repertoire of fatty acids produced by Yarrowia lipolytica.

The increasing demand for palm oil has drastic effects on the ecosystem, as its production is not sustainable. To that end, developing a sustainable alternative to fatty acids and oils is urgent and of utmost interest. Oils produced by oleaginous yeasts present a promising solution, particularly because the fatty acid profile of the oil produced by these yeasts is comparable to that of plant-based oils and fats. The fatty acid composition of the oil determines its physiological properties, thereby determining its potential applications. Accordingly, the production of microbial oil with an optimal composition profile for a specific application is of great importance. In this study, we evaluated the variation that occurred in fatty acid composition due to different cultivation parameters (temperature, C/N ratio, carbon, and nitrogen sources) and applied genetic modifications to improve the lipid accumulation of Cutaneotrichosporon oleaginosus and Yarrowia lipolytica. We showed that specific fatty acid profiles associated with a particular application can be obtained by carefully selecting the microorganism and cultivation conditions.

Abstract Microbial lipases are widely used in biotechnology and industrial processes. Yarrowia lipolytica exhibits strong lipolytic activity and optimizing fermentation conditions is crucial for efficient enzyme production. This research investigated lipase production of some newly isolated Yarrowia strains, including Yarrowia lipolytica, Yarrowia divulgata , and Yarrowia yakushimensis . The Y. lipolytica 854/4 strain has reached the highest lipase activity after 3 days of fermentation, whereas other isolates require a longer fermentation time of 6–7 days. The lipase synthesis was not significantly affected by three tested cell count (10 6 , 5*10 6 and 10 7 CFU mL −1 ) during optimization of the initial inoculum concentration. Different concentrations of Triton X-100 and Tween 80 supplementation enhanced the lipase production, although the extent of this effect varied from strain to strain. These findings highlight Y. yakushimensis as a promising species, in addition to Y. lipolytica for industrial application involving lipase production.

Waste from the fat-processing industry represents a challenging stream due to its physicochemical properties and environmental impact. Valorization through recovery and reuse offers ecological, economic, and social benefits. This study focused on mushy lipid residues generated during cold pressing of oilseeds (sunflower, flax, blue poppy, hemp, black cumin, and walnut) and evaluated their potential for lactone biosynthesis. The waste was analyzed for protein and fat content, while fatty acid profile, acid and peroxide values, oxidation stability, and health-related indices characterized the extracted oils. Polyphenol content and antioxidant activity of the residues were also determined. Subsequently, the waste was used as a substrate in biotransformation processes with Lactiplantibacillus plantarum and Yarrowia lipolytica. The results showed high protein (13.1–19.4%) and fat levels (65.0–77.3%) across all residues. The lipid fractions were rich in monounsaturated and polyunsaturated fatty acids, comprising nearly 90% of the total fatty acids, with oleic and linoleic acids being the dominant components. These features highlight their strong valorization potential, particularly for the microbial synthesis of aroma-active lactones. Under the applied conditions, the production of γ-dodecalactone and δ-decalactone reached 0.76 g/L and 1.62 g/L, respectively, confirming the suitability of cold-press residues as substrates for sustainable biotechnological applications.

Worldwide, aquaculture is among the fastest-growing food-producing sectors. To boost the health and growth of aquaculture organisms, microbial supplements are utilized. In particular, yeasts with desirable amino acid and lipid profiles are appealing options for inclusion in aquaculture feed. Besides Ascomycetous yeasts (mainly Saccharomyces cerevisiae, Debaryomyces hansenii, and Yarrowia lipolytica), two Basidiomycetous genera- Please consider reducing length of dashRhodotorula and Phaffia and their derived products (β-glucans, mannan oligosaccharides, and pigments) are used in the industry. They function as probiotics, feed additives, nutritional supplements, growth promoters, pigment enhancers, immunomodulators, immunostimulants, pathogen protectants, and stress relievers. They also enhance digestion, serum biochemical parameters, antioxidant capacities, meat quality, overall yields, and market value of aquaculture species. Since large quantities of yeast biomass can be produced by cultivating on inexpensive substrates, incorporating them into aquaculture feed offers a sustainable way to meet nutritional needs, increase productivity, and promote the overall health of aquaculture species.

Biocatalytic Deacetylation of Crystalline Chitin via Engineered <i>Yarrowia lipolytica</i>

Transportation relies heavily on fuel, which is mostly derived from fossil fuels. Fossil fuel comes from unsustainable sources. Such sources that consistently supply fuel are necessary. Oil seeds, lipid-accumulating microbes and inedible plant biomass might be better substitutes. The procedure of cultivating plant sources is laborious. Oleaginous yeast, a type of yeast that accumulates lipids inside the cells, may be a promising source. In terms of lipid buildup, Yarrowia lipolytica, an unconventional and well researched yeast, occurs with a range of carbon sources, including waste cooking oils, dairy industry waste, non-edible oil seeds, free fatty acids and numerous other media. This yeast accumulates lipids toward the conclusion of the log phase and the beginning of the stationary phase, under the lower nitrogen concentrations. A lot of research has been done on the genetics of lipid accumulation in Y. lipolytica, and efforts are being made to control the β-oxidation process for increased lipid accumulation in yeast cells. Through the process of transesterification, the recovered lipid from the yeast is transformed into fatty acid methyl esters, ie., biodiesel. Yeast biodiesel and petroleum diesel have similar qualities. Biodiesel blends are widely used in businesses and transportation, as they can be obtained based on the needs. Research on these oleaginous yeasts for their lipids as a source of biodiesel is being undertaken by numerous academic institutions and private businesses. The difficulty that needs to be successfully addressed, is making yeast-based biodiesel accessible and affordable.

Volatile fatty acids (VFAs) derived from organic waste offer promising and cost-effective carbon sources for the production of microbial lipids. This study demonstrates the significant influence of nitrogen nutrition on cell proliferation and microbial lipid synthesis in Yarrowia lipolytica during high-concentration acid cultivation. Further investigations into nitrogen sources revealed that NH4Cl and urea are suitable options for cultivating Y. lipolytica to produce microbial lipids, resulting in lipid yields ranging from 2.00 to 2.50g/L. Moreover, pH fluctuations were found to be influenced by both the nitrogen source and acid utilisation, with pH adaptation helping alleviate acid inhibition caused by high-concentration VFAs. Under optimised cultivation conditions, the highest yield of microbial lipids reached 4.00g/L, accompanied by a dry cell weight of 9.91g/L and a microbial lipid content of 40.37%, consisting predominantly of C16 ~ 18 fatty acids. These findings highlight the central role of nitrogen metabolism and pH adaptation in enhancing VFA assimilation, offering guidance for cost-effective microbial lipid production from organic waste streams.

Sustainable high-titer production of β-elemene in Yarrowia lipolytica via modular engineering, pathway refactoring, and a chimeric germacrene A synthase

Developing a Yarrowia lipolytica platform for conversion of mannitol-containing waste streams.

Machine learning-assisted rational design and evolution of novel signal peptides in Yarrowia lipolytica.

Alternative protein and microbial food production with Yarrowia lipolytica: precision, biomass and traditional fermentation strategies

The key role of Cytochrome P450 and ALDH in citrus flavor (+)-valencene biotransformation to (+)-nootkatone with Yarrowia lipolytica D21173