Wound Healing In Albino Rats Research Articles

Eggshell membrane and green seaweed (Ulva lactuca) micronized powders for in vivo diabetic wound healing in albino rats: a comparative study

BackgroundNonhealing diabetic wounds are a serious complication associated with extremely lethargic wound closure and a high risk of infection, leading to amputation or limb loss, as well as substantial health care costs and a poor quality of life for the patient. The effects of either eggshell membrane (ESM) and green seaweed (Ulva lactuca) extracts alone or in combination were evaluated for in vivo skin wound healing in a rat model of induced diabetes.MethodsMicronized powders of waste hen ESM, Ulva lactuca, and their 1:1 mixture were prepared using regular procedures. The mechanical, electrical, and surface morphology characteristics of powders were examined using direct compression, LCR-impedancemetry, and scanning electron microscopy. The effect of ESM, Ulva lactuca, and their mixture as compared to standard Dermazin treatments were evaluated on wounds inflicted on male Wistar Albino rats with induced diabetes. Quantitative wound healing rates at baseline and at 3, 7, 14, and 21 days of treatments among all rat groups were conducted using ANOVA. Qualitative histological analysis of epidermal re-epithelization, keratinocytes, basement membrane, infiltrating lymphocytes, collagen fibrines, and blood vessels at day 21 were performed using Image J processing program.ResultsCompressive strength measurements of tablets showed a Young’s modulus of 44.14 and 27.17 MPa for the ESM and ESM + Ulva lactuca mixture, respectively. Moreover, both samples exhibited relatively low relative permittivity values of 6.62 and 6.95 at 1 MHz, respectively, due to the porous surface morphology of ESM shown by scanning electron microscopy. On day 21, rats treated with ESM had a complete diabetic wound closure, hair regrowth, and a healing rate of 99.49%, compared to 96.79% for Dermazin, 87.05% for Ulva lactuca, 90.23% for the mixture, and only 36.44% for the negative controls. A well-formed basement membrane, well-differentiated epithelial cells, and regular thick keratinocytes lining the surface of the epidermal cells accompanied wound healing in rats treated with ESM, which was significantly better than in control rats.ConclusionGround hen ESM powder, a low-cost effective biomaterial, is better than Ulva lactuca or their mixture for preventing tissue damage and promoting diabetic wound healing, in addition to various biomedical applications.

The efficacy of topically applied Sappan wood (Caesalpinia sappan L.) ethanol extract during incision wound healing in albino rats.

Medical manifestations in the form of incisions, burns, and trauma will trigger a natural wound-healing process that involves complex interactions between cells. Brazilin and other secondary metabolites found in Sappan wood have numerous bioactive qualities, including anti-inflammatory, anti-cancer, and antioxidant properties. This study aimed to investigate the efficacy of Sappan wood (Caesalpinia Sappan L.) ethanol extract topically on the incision wound healing of albino rats. Twenty male rats were randomly assigned into five groups with four replications, i.e., (C-) was treated ointment-based, (C+) was treated with 10% povidone-iodine, (T1, T2, and T3 groups) were treated with Sappan wood extract concentration for 6.5%, 15%, and 30%, respectively. The treatment was topically administered to wounded areas twice a day for 15 days. Wound healing was evaluated histologically as the following parameters collagen deposition, polymorphonuclear neutrophils (PMN), angiogenesis, and fibrosis degree using H&E staining. IL-2 level was evaluated using the enzyme-linked immunosorbent assay (ELISA) method. Wound length reduction was calculated on days 8 and 15. As a result, the 6.5% (T1), 15% (T2), and 30% (T3) Sappan wood extract groups were improved significantly (p < 0.05) compared to ointment-based (C-) and povidone-iodine (C+) groups on the collagen deposition, PMN, angiogenesis, fibrosis degree, and IL-2 level. In particular, the 6.5% (T1) Sappan wood extract group was highlighted significantly (p < 0.05) compared to other groups, evidenced by the improvisation of wound healing parameters and reduction of wound length on days 8 and 15. In conclusion, a 6.5% Sappan wood extract revealed its applicability to improve incision wound healing in albino rats.

Efficacy of Sauropus androgynous Leaves Extract Gel on Burn Wound Healing in Albino Rats

Burns not only damage skin locally but generally affect the body system and have been related as the secondary cause of death. Burns can be effectively treated with a topical drug to prevent chronic inflammation. Burn wound healing is really important for ensuring overall health and well-being. This study aimed to determine the effect of Sauropus androgynous leaves extract gel on the number of fibroblasts, fibrocytes, and collagen density on burn wound healing in albino rats. A total of 50 male albino rats were randomly divided into five groups i.e. (C-) was normal skin, (C+) was skin burn treated with placebo, (T1) was skin burn treated with 2.5% of S. androgynous leaves extract gel, (T2) was skin burn treated with 5% of S. androgynous leaves extract gel, and (T3) was skin burn treated with 10% of S. androgynous leaves extract the gel. The amount of 25 albino rats’ skin samples were collected on the 8th day, and the remaining samples were collected on the 15th day. The results showed that 10% of S. androgynous leaves extract gel improved faster, as indicated by the increased number of fibroblasts, fibrocytes, and collagen density. This study can be concluded that 10% of S. androgynous leaves extract gel was the effective dosage to increase the number of fibroblasts, fibrocytes, and collagen density in burn wound healing.

Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats

This study aimed to evaluate the in-vivo burn wound healing process in albino rats treated using transdermal delivery nano ethosomal gel from ashitaba leaves. Ethosomal vesicles were formulated using soy lecithin, cholesterol, ethanol, water, and ashitaba leaf nanoparticles using the cold method. A total of 25 male rats were randomly divided into 5 groups, i.e., (C-) treated without nanoparticle extract, (C+) treated using 1% Silver Sulfadiazine®, treatment group treated nano ethosomal gel from ashitaba leaves with the respective doses were (T1) 1%, (T2) 2,5%, and (T3) 5%. Therapy was initiated on day 1 or after being induced by a burn wound for 14 days, twice a day. On the 15th day, a termination was carried out to take skin tissue from burn scars, then histopathology preparations were made with routine staining. Microscopic observations with an optical microscope on collagen, polymorphonuclear cell infiltration, angiogenesis, and re-epithelialization. The T3 group that was treated using 5% nano ethosomal gel showed the best burn wound healing, this may be caused by compounds in ashitaba leaf nanoparticles which have antioxidant, anti-inflammatory, and antibacterial effects, thus the use of transdermal delivery therapy of 5% ashitaba leaf nano ethosomal gel was effective for wound treatment burn on rat skin.

Wound Healing and Curative Properties of Alligator Pepper (Aframomum Melegueta) seed extract in Albino Rats (Rattus Norvegicus)

The aim of this study was to determine the effects of Alligator pepper, Aframomum melegueta, seed extract on wound healing in albino rats. Four treatment groups of eight albino rats each were used for the experiment. Group 1 were given feed and water only. Group 2, 3 and 4 were exposed to a reference drug (Cicatrin), 10% and 20% of the extract respectively for 21 days. Excision wounds of 30mm were made on dorsal thoracic central region of the rats. Serum from blood collected from the dorsal aorta of sacrificed rats were analysed for oxidative stress biomarkers (TNF-α, MDA and SOD) using standard methods. The tissue samples from the wounds were collected and histhopathologically analysed under a light microscope. The decrease in wound size was measured using an electronic Vernier Calliper at intervals of two days. The values of TNF-α ranged from 87pg/ml to 136pg/ml while those of MDA ranged from 0.92 to 3.19µmol/L. The maximum and minimum values of SOD recorded were 25.15U/mg protein and 12.6U/mg protein respectively. The smallest wound size value, 5.5mm, was measured in Group 3 on day 19 of the experiment. Behavioural changes were observed which included lethargy and anorexia. The histopathological study of the extract groups showed the formation of new tissues, keratinized substances and hair follicle. The results obtained from the wound measurements supported the conclusion that the extract had a better effect on the wound healing in experimental animals.

Wound Healing Study of Panchavalkal Ointment in Wistar Albino Rats

Introduction: Panchavalkal is a well-known Ayurvedic poly-herbal formulation that has been reported to be used against inflammation, to clean ulcer, wound.&#x0D; Aims and Objectives: To investigate the wound healing activity of Panchavalkal ointment.&#x0D; Materials and Methods: Wistar strain albino rats of either sex weighing 200±20 g were used for the experiments divided in four groups each consisted of six rats.&#x0D; Statistical Analysis: One-way analysis of variance (ANOVA) was used to compare the mean values of quantitative variables among the groups followed by Dunnett’s multiple ‘t’ test&#x0D; Observations &amp; Results: Sesame oil and Panchvalkal ointment showed almost similar wound healing effects in comparison to control group.&#x0D; Discussion: Panchavalkal ointment showed statistically highly significant percentage of contraction of excision wound area compared to the normal control. Epithelization period was significantly decreased in oil and Panchavalkal ointment treated group.&#x0D; Conclusion: Panchavalkal ointment decreased the pain, tenderness, redness and swelling that helps to control infection and enhanced the rate of wound healing in albino rats.

Clinicopathological Studies on the Effect of Insulin as Spray on Diabetic Wound Healing in Albino Rats

Clinicopathological Studies on the Effect of Insulin as Spray on Diabetic Wound Healing in Albino Rats

The effectiveness of ethanolic extract of propolis on wound healing in albino rats

The effectiveness of ethanolic extract of propolis on wound healing in albino rats

Histological Evaluation of the Emerging Role of Adipose Stem Cells-Derived Exosomes in Cutaneous Wound Healing in Albino Rats

Prolonged healing and scar formation are two major challenges in treating wounds, resulting in physical, mental and socioeconomic burden to the affected subjects. Exosomes (Exos) are naturally secreted vesicles playing a vital role in the transfer of RNA to neighboring or distant recipient cells and there are numerous on-going studies on their role of in tissue regeneration and wound healing.Aim of the work: Evaluation of the efficiency of Exos derived from adipose stem cells on cutaneous wound healing in adult male albino rats.Materials and Methods: Thirty-four rats were divided into three groups; Group I: control. Group II: a dorsal cut wound (1.5 × 1.5 cm) was done to each rat. Group III: the same wound was done and rats were injected with 200μ PKH26-labeled Exos intravenously. Rats were sacrificed after 21 days. Skin sections were subjected to hematoxylin and eosin (HandE), Mallory's trichrome and orcein stains, alpha smooth muscle actin (α-SMA) and Ki67 immunohistochemical stains.Results: Skin sections from group II showed scar formation, lost epidermal layers and hair follicles. There was significant increase in collagen fibers and α-SMA immunoreaction, with significant decrease in elastic fibers and Ki67 immunoexpression compared to the control. In group III, the skin revealed almost normal histology, significant decrease in collagen fibers and α-SMA, with significant increase in elastic fibers and Ki67 immunoreaction compared to group II.Conclusion: Exos injection has demonstrated a beneficial effect on cutaneous wound healing and could prevent scar formation in adult male albino rats.

Assessment of the Topical and Systemic Effects of Omega-3 on Oral mucosal wound healing in albino rats: A Histopathological and Biochemical study

Assessment of the Topical and Systemic Effects of Omega-3 on Oral mucosal wound healing in albino rats: A Histopathological and Biochemical study

Evaluation of dipeptidyl peptidase-4 enzyme inhibition by some commonly used gliptins on cutaneous wound healing in albino rats

Background: Dipeptidyl peptidase-4 enzyme not only inhibits incretins but also many other substrates and has wide distribution. Inhibition of this enzyme may have many implications, one of which is to improve wound healing. Aims and Objectives: This aim of this study is to evaluate wound healing property of linagliptin, saxagliptin, sitagliptin, and vildagliptin on excised wounds in non-diabetic Wistar albino rats. Materials and Methods: Some commonly used gliptins, linagliptin, saxagliptin, sitagliptin, and vildagliptin were assessed for pro-wound healing property on excised wound in non-diabetic Wistar albino rats. Wound healing was assessed by measuring the scar area at 4,8,12 & 16 days and the time taken for the complete scar formation.On the 16th day, rats were sacrificed, wound tissue biopsy was taken for assessing granulation tissue and serum was subjected for tumor necrosis factor-1α, interleukin-6, inducible nitric oxide synthase, hydroxyproline and glucose estimation. Results: Pro-healing property was not uniform among various tested gliptins, only linagliptin and sitagliptin were found to have improved wound healing potential in rat excised wound. Conclusion: Gliptins show variable effect on wound healing.

The Effect of Mesenchymal Stem Cells and Chitosan Gel on Full Thickness Skin Wound Healing in Albino Rats: Histological, Immunohistochemical and Fluorescent Study.

BackgroundWound healing involves the integration of complex biological processes. Several studies examined numerous approaches to enhance wound healing and to minimize its related morbidity. Both chitosan and mesenchymal stem cells (MSCs) were used in treating skin wounds. The aim of the current work was to compare MSCs versus chitosan in wound healing, evaluate the most efficient route of administration of MSCs, either intradermal or systemic injection, and elicit the mechanisms inducing epidermal and dermal cell regeneration using histological, immunohistochemical and fluorescent techniques.Material and MethodsForty adult male Sprague Dawley albino rats were divided into four equal groups (ten rats in each group): control group (Group I); full thickness surgical skin wound model, Group II: Wound and chitosan gel. Group III: Wound treated with systemic injection of MSCs and Group IV: Wound treated with intradermal injection of MSCs. The healing ulcer was examined on day 3, 5, 10 and 15 for gross morphological evaluation and on day 10 and 15 for histological, immunohistochemical and fluorescent studies.ResultsChitosan was proved to promote wound healing more than the control group but none of their wound reached complete closure. Better and faster healing of wounds in MSCs treated groups were manifested more than the control or chitosan treated groups. It was found that the intradermal route of administration of stem cells enhanced the rate of healing of skin wounds better than the systemic administration to the extent that, by the end of the fifteenth day of the experiment, the wounds were completely healed in all rats of this group. Histologically, the wound areas of group IV were hardly demarcated from the adjacent normal skin and showed complete regeneration of the epidermis, dermis, hypodermis and underlying muscle fibers. Collagen fibers were arranged in many directions, with significant increase in their area percent, surrounding fully regenerated hair follicles and sebaceous glands in the dermis of the healed areas more than in other groups.ConclusionMSCs enhanced the healing process of wound closure more than chitosan gel treatment. Furthermore, MSCs injected intradermally, were more efficient in accelerating wound healing than any other mode of treatment.

Effect of Dehydrozingerone, a half analog of curcumin on dexamethasone-delayed wound healing in albino rats

Oxidative stress is triggered by the wound which results in the production of reactive oxygen species (ROS), thereby delaying normal wound repair. Therefore, it is important to reduce the level of ROS to improve healing. A known antioxidant, dehydrozingerone (DHZ) was synthesized and selected for the study. The authors aimed to investigate the wound healing action of topical (100 mg/wound) and systemic (100 mg/kg, p. o.). DHZ on different wound models in normal and dexamethasone (DEX)-suppressed healing. Topical DHZ showed a significant (P < 0.05) rise in tensile strength when compared to control in normal healing. Significant (P < 0.05) wound closure was observed from 3 to 9 days in DHZ oral and gel treated groups. There was a significant (P < 0.05) rise in hydroxyproline content with the DHZ treated groups when compared to control. Systemic DHZ exhibited a significant (P < 0.05) increase in lysyl oxidase (LO) levels of 3.73 ± 0.15 nmol of H(2)O(2) when compared to control. In DEX-suppressed healing, showed good pro-healing activity with respect to the parameters mentioned above. DHZ treatment exhibited a parabolic dose response of ROS inhibition with a plateau effect at 75 μM. There was a steady and constant increase in the % NO inhibition with increasing doses of DHZ. Oral DHZ is effective in accelerating the healing process in both normal and dexamethasone-suppressed wounds. Our study suggests that DHZ (half analog of curcumin) supplementation reduces the steroid-induced delay in wound healing.

Wound Healing Profile of Asparagus Racemosus (Liliaceae) Wild

Asparagus racemosus is a well known plant of medicinal value, with proved antioxidant, anti-inflammatory and immunomodulatory properties. Antioxidants are reported to enhance wound healing. The present study is aimed to investigate the wound healing profile of Asparagus racemosus on incision and excision wound models in rats. The aqueous extract of the roots of Asparagus racemosus is made use of to study the effect on wound healing in albino rats using incision and excision wound models in 200mg/kg and 400mg/kg orally for 10 to22 days. The skin breaking strength, epithelialisation period, wound contraction rate were estimated. The extract showed significant improvement in the epithelialisation period, remarkable enhancement of wound contraction rate, increased skin breaking strength in the test groups suggesting the possible utility of this plant to enhance wound healing

Effect of crushed garlic extract on wound healing in albino rats

Garlic extract is gaining increasing acceptance as an antimicrobial and anti-inflammatory agent and as a folk remedy on wound healing. We studied the effect of crushed garlic extract on wound healing in albino rats. 20 male albino rats had a linear 3 cm incision made over skin of back. The animals were randomly divided into two experimental groups, as control and treatment. Animals of control group received topical cold cream once a day from beginning of experiments to complete wound closure. Animals of treatment group treated topically by crushed garlic extract cream at the same time. For computing the percent of wound healing, the area of wound was measured at the beginning of experiments and 2, 4, 6, 8, 10, 12, 14 and 16 days after that. There was statistically significant difference between treatment and control animals in some of days (P

Effect of propolis on wound healing in albino rats

Propolis is a resinous, sticky substance that bees collect from conifer buds and use to repair cracks in their hives. Propolis was used in folk medicine as early as 300 BC as an anti-inflammatory drug and wound healing agent. We studied the effect of Propolis on wound healing in albino rats. Twenty male albino rats had a linear 3 cm incision made over skin of back. The animals were randomly divided into two experimental groups, as control and treatment. Animals of the control group received topical cold cream once a day from beginning of experiments to complete wound closure. Animals of the treatment group were treated topically by Propolis cream at the same time. For computing the percent of wound healing, the area of wound was measured at the beginning of experiments and 2, 4, 6, 8, 10, 12, 14 and 16 days after that. There was no statistically significant difference between treatment and control animals in most of days. In spite of some beneficial effects of Propolis, it had no effect on the rate of wound closing in an incisional wound model in rats.

Evaluation of topical Matricaria chamomilla extract activity on linear incisional wound healing in albino rats

In this investigation, the effect of Matricaria chamomilla extract on linear incisional wound healing was studied. Thirty male Wistar rats were subjected to a linear 3 cm incision made over the skin of the back. The animals were randomly divided into three experimental groups, as control, olive oil, and treatment. Control group did not receive any drug or cold cream. Olive oil group received topical olive oil once a day from beginning of experiments to complete wound closure. Treatment group were treated topically by M. chamomilla extract dissolved in olive oil at the same time. For computing the percentage of wound healing, the area of the wound measured at the beginning of experiments and the next 2, 5, 8, 11, 14, 17, and 20 days. The percentage of wound healing was calculated by Walker formula after measurement of the wound area. Results showed that there were statistically significant differences between treatment and olive oil animals (p < 0.05) in most of the days. We conclude that the extract of M. chamomilla administered topically has wound healing potential in linear incisional wound model in rats.

METHIONINE and cystine in wound healing in albino rats.

Journal Article Methionine and Cystine in Wound Healing in Albino Rats Get access Nutrition Reviews, Volume 11, Issue 4, April 1953, Pages 124–126, https://doi.org/10.1111/j.1753-4887.1953.tb01310.x Published: 01 April 1953

An experimental study of the effects of Matricaria chamomilla extract on cutaneous burn wound healing in albino rats

Previous studies conducted on the anti-inflammatory, antimicrobial and antioxidant effects of Matricaria chamomilla (chamomile) extract led us to study the effect of topical chamomile extract on burn wound healing in albino rats. Thirty male albino rats (250–300 g) were randomly divided into three groups, as control, vehicle, and treatment. Second–degree burning was induced in 20% of whole surface area of animal body by placing the back of animal into boiling water for 8s. Animals of control group received no treatment. Animals of vehicle and treatment groups were treated topically by olive oil and extract dissolved in olive oil twice a day respectively from the first day of burn induction to complete wound healing. The percentage of wound healing was calculated weekly. The results showed that there was significant difference (p < 0.05) between vehicle and treatment groups. So we concluded that the chamomile extract in the form of rubbing oil had a good potential for acceleration of burn wound healing in rats.

The influence of some azoles on wound healing in albino rats

Objective: To investigate the effect of azoles viz metronidazole (MTZ), tinidazole (TNZ), ketoconazole (KCZ), and fluconazole (FLZ) on resutured incision, excision, and dead space wounds in albino rats. Materials and methods: Resutured incision, excision, and dead space wounds were inflicted in albino rats of either sex under light ether anesthesia, taking aseptic precautions. Control animals received vehicle, and other groups received MTZ, TNZ, KCZ, and FLZ orally for a period of 10 days in incision and dead space wounds, whereas in excision wounds till their complete closure. On the 11th day, after estimating breaking strength of resutured incision wounds (under anesthesia), animals were sacrificed and granulation tissue removed from dead space wounds to estimate breaking strength, hydroxyproline content, quantification of granulation tissue, and histological studies in control and treated groups. In the excision wound model, wound closure rate, epithelization time, and scar features were studied from the day of wounding till the day of scab falling off with no residual raw area. Results: Except ketoconazole, rest of the azoles significantly (P Conclusion: Metronidazole, tinidazole and fluconazole promote wound healing, whereas ketoconazole does not do so.