Rockburst, one of the leading types of disaster in mining and rock engineering causing serious injuries and the loss of property, frequently occurs, involving various features and complex evolutionary mechanisms. Compared to rockbursts occurring at mining faces, those occurring in main roadways cause more serious problems for mine production. This paper first analyzes the characteristics of rockbursts in main roadways using two case studies involving the Gaojiapu and Cuimu coal mines. The causes of rockbursts in main roadways were studied using microseismic monitoring, energy density cloud maps, and seismic velocity tomography. During the mining of the 22306 working face in the Cuimu coal mine, targeted measures, such as deep-hole blasting of the roof strata and deep-hole blasting of the coal seam, were implemented to prevent rockbursts in the main roadways. The effectiveness of these measures was verified through long-term analysis of tremor activities. The study found that the influence of mining at two working faces on both sides of main roadways was significantly greater than that from a single-sided working face. The intensity of the tremor activities occurring near the main roadways was correlated with the distance from the working face to the main roadways. The closer the working face was to the main roadways, the stronger the tremor activities were near the main roadways. According to the distribution range of the tremors, the influence area of working face mining exceeded 800 m, with tremors distributed linearly along the main roadways. Even five months after the completion of working face mining, there were still a large number of tremors near the main roadways, which gradually disappeared after another five months. Mining activities were the main reason for the occurrence of main roadway rockbursts and the stress concentration within the main roadways themselves was another reason for the occurrence of rockbursts. The influence of working face mining could be reduced by deep-hole blasting of roof strata and the stress concentration within main roadways themselves could be reduced by large-diameter drilling. Those joint preventive measures effectively prevented the occurrence of rockbursts in main roadways. This study is of important theoretical and practical significance for further studies of rockburst mechanisms and prevention in regard to main roadways in coal mines, and the findings are significant in terms of the enhancement of safety in coal mines.