Background and objective: Job fatigue is considered one of the factors affecting the performance and the productivity of workers in work environments. Therefore, the objective of the present study was to investigate the relationship between job fatigue and its dimensions with fine motor skills in the production line workers of Sabzevar Cable Car Company. Participants and methods: One hundred and forty-eight workers of the production line of Sabzevar Car Company were selected as a sample. The level of occupational fatigue of workers was measured using the standard questionnaire of Excessive Occupational Fatigue. In addition, manual and sorting skills of workers were estimated through Board test. In order to analyze the research data, Spearman's correlation coefficient test was used. Results: The results of the Spearman correlation coefficient test showed that there were negative and significant relationships between job fatigue and the components of hand and finger dexterity skill, including manual skills (r = 0.188, P ≤ 0.022) and sorting skill r (r = -0.246, P ≤ 0.003). Also, a statistically significant relationship was observed between the dimensions of fatigue and coping skills (P≤0.05). Conclusion: According to the results of the present research study, we recommend that employers prevent the occurrence of worker fatigue in industrial environments through providing conditions such as shift breaks and performing short-term activities with low intensity. Otherwise, in order to prevent accidents and the production of defective products, tired workers should refrain from performing fine manual activities such as assembling.