The level of job satisfaction is one of the key pushing factors for improving employee productivity and efficiency in every organization. On the other hand job stress is pulling factor of job satisfaction, productivity and efficiency in the long run. Therefore, two are two extreme variables result into two opposite outcomes However, levels of job stress and job satisfaction varies as per the personalities of employees such as internal and external personalities and gender wise. The present study emphasized on the understanding the relationship between job stress, job satisfaction and work locus of control and variation between genders and type of locus of control among the 150 selected IT professionals in Bangalore. The outcome of the study evidence that, internal locus of control leads to higher job satisfaction of the employees in IT sector irrespective of gender variation and external locus of control causes lower job satisfaction. There is existence of negative correlation between job stress and external locus of control on job satisfaction. Besides, the study found insignificant variation in workplace stress, job satisfaction, and work locus of control. The study also observed high job satisfaction of the employees in IT sector with regarding to promotion, supervision, nature of work and support from colleagues and dissatisfaction regarding remuneration and operating conditions.