Wood Sections Research Articles

Effective section on grapevine plants (Vitis vinifera L.) attacked by Xylotrechus arvicola Olivier (Coleoptera: Cerambycidae) grubs: Influence and effects on its resistance

Xylotrechus arvicola Olivier 1795 (Coleoptera: Cerambycidae) is an insect pest that affects Vitis vinifera L. 1753 (Vitales: Vitaceae) plants in the main wine-producing regions of Spain. X. arvicola larvae bore into grapevine wood, causing both direct damage (ingestion of vascular tissues) and indirect damages (introduction of wood fungi) to the plant. The aim of research was to evaluate the effective section of wood damaged by larvae and assess its resistance capacity through compression tests and loading and breaking times. Compressive tests (on trunks) and flexural tests (on branches) were performed to evaluate the effective section. Trunk samples exhibited a higher effective section than branches samples, with effective section percentages ranging from 91.49 % to 93.53 % in trunks and decreasing from 84.91 % to 86.95 % in branches. Both loading times (Time 1) and breakage times (Time 2) increased with the effective section of the wood, although these times were lower in damaged wood samples of both trunks and branches. Additionally, significant differences were observed in the interactions between loading time x effective section and breakage time x effective section in dry trunks. This indicates a stronger relationship between the effective section and increased resistance in trunks. The results suggest that, in ‘Tempranillo’ variety, branches with a lower effective section are more prone to breakage when affected by X. arvicola larvae, whereas trunks, with a greater effective section, maintain better stability. This research should be continued with the evaluation of other vine varieties and different years of X. arvicola attacks, as the current findings are based on a single variety (‘Tempranillo’) over a period of ten years.

Resource Sharing between the Invasive Sirex noctilio and Native Woodborers and Beetles in Pinus Plantations.

Sirex noctilio, a European woodwasp, occasionally shares resources with the native S. nitobei and other colonizers in northeast China. The impact of its coexistence on individual species remains unclear. Random sampling was conducted to assess the patterns and extent of insect co-colonization across various spatial scales. Additionally, we analyzed wood sections to determine the density, adult size, and distribution of the two Sirex species. Spatial scales revealed negative associations (Asemum striatum and Phaenops sp.) and neutral ones (Ips acuminatus) between woodwasps and other co-colonizers. Clustering of woodwasps and Phaenops sp. occurred at a small scale (0-7.3 m). Regression analysis showed a positive correlation between the chance of woodwasp attacks and past attacks on the same host, with little impact from other colonization factors. The distribution and body size of S. noctilio within the tree appeared unaffected by S. nitobei's presence. In the presence of S. noctilio, S. nitobei tended to lay eggs in damaged sections. At the stand level, the overall impact of S. noctilio on S. nitobei population density is likely positive because S. nitobei prefer weaker trees, a preference potentially influenced by initial attacks from S. noctilio on healthier hosts.

Radial Variation and Early Prediction of Wood Properties in Pinus elliottii Engelm. Plantation

To explore the radial variation in wood properties of slash pine (Pinus elliottii Engelm.) during its growth process and to achieve the early prediction of these properties, our study was carried out in three slash pine harvest-age plantations in Ganzhou, Jian, and Jingdezhen, Jiangxi province of South China. Wood core samples were collected from 360 sample trees from the three plantations. SilviScan technology was utilized to acquire wood property parameters, such as tangential fiber widths (TFWs), radial fiber widths (RFWs), fiber wall thickness (FWT), fiber coarseness (FC), microfibril angle (MFA), modulus of elasticity (MOE), wood density (WD) and ring width (RD). Subsequent systematic analysis focused on the phenotypic and radial variation patterns of wood properties, aiming to establish a clear boundary between juvenile and mature wood. Based on determining the boundary between juvenile and mature wood, a regression equation was used to establish the relationship between the properties of juvenile wood and the ring ages. This relationship was then extended to the mature wood section to predict the properties of mature wood. Our results indicated significant differences in wood properties across different locations. The coefficients of variation for RD and MOE were higher than other properties, suggesting a significant potential for selective breeding. Distinct radial variation patterns in wood properties from the pith to the bark were observed. The boundary between juvenile and mature wood was reached at the age of 22. The prediction models developed for each wood property showed high accuracy, with determination coefficients exceeding 0.87. Additionally, the relative and standard errors between the measured and predicted values were kept below 10.15%, indicating robust predictability. Mature wood exhibited greater strength compared to juvenile wood. The approach of using juvenile wood properties to predict those of mature wood is validated. This method provides a feasible avenue for the early prediction of wood properties in slash pine.

Bursaphelenchus glaucae n. sp. isolated from larval faeces of a longhorn beetle, Aeolesthes chrysothrix chrysothrix (Bates), in its pupal chamber constructed in the dead wood of Quercus glauca Thunb. from Japan

Summary A Bursaphelenchus species belonging to the hofmanni group (corneolus subgroup) was isolated from a small piece of larval faeces of the longhorn beetle Aeolesthes chrysothrix chrysothrix in its pupal chamber. The chamber was constructed in a dead wood section of Quercus glauca obtained from a live tree. The material was obtained during a management procedure of a garden tree to remove the dead part from live tree. The species is typologically characterised by its three-lined lateral field, the position of its secretory-excretory pore overlapping with the metacorpus anterior half, a very small spicule with distinctive broad and rounded condyles, a flattened trapezoid-shaped rostrum and small flattened cucullus, the presence of seven (P1, P2 and P3 papilliform and P5 gland) male genital papillae, a female vulva with a side flap and female tail forming an elongate conoid with a strongly ventrally curved posterior half and narrowly rounded or digitate tip. The species is typologically close to B. corneolus, sharing a small cucullus and female tail shape, but can be distinguished from the other species by the position of the secretory-excretory pore and the spicule rostrum shape. Phylogenetically, the new species forms a well-supported clade with B. corneolus, but can be distinguished by its clearly separable phylogenetic status inferred from SSU and D2-D3 LSU sequences and the difference in the ITS sequence. The new species is described as B. glaucae n. sp. Although the nematode was isolated from the larval faeces of A. c. chrysothrix, it has not been directly isolated from the insect body to date, thus, the carrier insect of the nematode has not been determined.

Relationships between diameter growth and functional wood anatomy in Eucalyptus globulus clones

Aim of study: We aimed to 1) analyze the variability of wood hydraulic anatomical traits in 10 clones of E. globulus Labill. with different growth rates, and 2) determine whether the magnitude of diameter growth affects the relationships between anatomical variables and diameter at breast height (DBH). Area of study: 25-year-old common garden trial in Balcarce, Buenos Aires, Argentina. Material and methods: We measured vessel diameter and number per unit area in transverse histological sections of stem wood, and calculated the proportion of vessel lumens, vessel composition (S), and theoretical specific hydraulic conductivity (Ks) of 10 E. globulus clones of high (HG) and low (LG) mean growth rates (measured as DBH) under field conditions. Main results: There was a difference in the range of variability in hydraulic anatomy between HG and LG clones, with LG clones showing a wider range. HG clones had wood with larger and fewer vessels and higher S compared to LG clones, with similar Ks between both growth groups. No clear or strong trends were observed between wood anatomy and DBH within the HG and LG groups, but across all clones a high correlation (Spearman coefficient r; p<0.001) was observed between vessel number ‒ DBH (r= -0.68), and S ‒ DBH (r= 0.74). These correlations were driven by contrasting mean values of both growth groups. Research highlights: Commercial E. globulus clones present a relatively large variation in anatomical and hydraulic strategies. However, in contrast to what is postulated for various woody species, there was no clear relationship between theoretical hydraulic efficiency and individual diameter growth rate in the genotypes studied.

Attenuation coefficient for Agarwood and shade tree sections at 59.6keV and 356keV

Attenuation coefficient for Agarwood and shade tree sections at 59.6keV and 356keV

Phloem Sap and Wood Carbon Isotope Abundance (δ13C) Varies with Growth and Wood Density of Eucalyptus globulus under Nutrient Deficit and Inform Supplemental Nutrient Application

Eucalyptus globulus, commonly known as blue gum or southern blue gum, is a tall, evergreen tree endemic to southeastern Australia. E. globulus is grown extensively in plantations to improve the sustainability of timber and fibre production across Australia. Sustainable forest management practices necessitate the consideration of ‘off-site’ carbon and ecological footprints. Pursuing optimal supplemental nutrient application and maximum growth rates is therefore critical to the establishment of a sustainable timber and fibre production industry. Biological indicators that can predict growth responses are therefore of extreme value. We investigated the carbon isotope abundance of wood cellulose (δ13Ccel) in E. globulus to determine potential relationships with the carbon isotope abundance of phloem sap (δ13Cphl) where the trees were subjected to different level of nutrient availability. This study also sought to determine the effect of nutrient additions on the growth of the E. globulus and to quantify the relationship between the volumetric growth of wood and δ13Ccel. Phloem sap and wood cores were collected from trees within study plots which were subjected to seven nutrient treatments over a two-year period in a monoculture E. globulus plantation in South Australia. Phloem sap was collected using the razor blade technique and wood cores were collected using a stem borer. The carbon isotope abundance (δ13C) of phloem sap and wood grown in the radial direction of the stem were determined. The basic and dry densities of wood were determined, and their relationships with phloem and wood δ13C were established. The δ13Cphl was significantly correlated with δ13Ccel. The relationship between δ13Ccel and the wood density of the respective wood sections was significant but did not consistently show the same pattern. There was no significant variation in basic density observed along the radial direction of the stem wood of the short-rotation E. globulus trees. A positive correlation was observed between δ13Ccel and the wood basic density, but the relationship was not consistent along the radial direction of the stem. However, positive correlations were observed between δ13Ccel and the air-dry density of respective wood sections. The relationship between phloem and wood δ13C and the relationship between δ13C and wood density along the radial direction of the stem needs to be considered while monitoring forest growth under nutrient- and water-limited conditions.

Machine learning-based wood anatomy identification: towards anatomical feature recognition

Summary Computer vision-based wood identification has been successfully applied to recognize tree species using digital images of wood sections or surfaces. However, this image-to-species approach can only recognize a limited number of species due to two main reasons: 1) the lack of a good reference database requiring high-quality standardized images from multiple individuals of hundreds or even thousands of traded timber species, and 2) species not included in the reference database cannot be identified without expert knowledge. Another bottleneck is that the feature extraction process used by these species recognition approaches is a black box, thereby creating a discrepancy between machine learning features and wood anatomical features. This discrepancy prevents wood anatomists from understanding how these machine-learning algorithms work. Here, we survey currently existing methods used in feature extraction, classification, and deep learning methods applied in wood identification along with their pitfalls and opportunities. As an example of how the field could move forward, we launch the idea of building an image-to-features-to-species identification approach based on microscopic wood images as well as text files comprising wood anatomical descriptions. If we can manage machine learning-based algorithms to recognize the main wood anatomical traits that experts use to identify species in a (semi-)automated way, this would boost wood identification in two ways: (1) extensive reference databases for each species would become less crucial as the databases are ordered at the trait level, (2) timber identification would become more feasible for species that have not yet been included in the reference database as long as wood anatomical descriptions are available.

Research data in paleobotany: petrographic thin sections fossil wood dataset

Objective: This study was aimed at analyzing and disseminating the fossil wood collection data stored in the thin slide sections of the paleobotany collection of the Department of Paleontology and Stratigraphy of the Institute of Geosciences of a university in Southern Brazil. Thin sheets of petrified wood are described. Research data used in geosciences specifically seek to develop a model for the use of this type of sheet in paleobotany, enabling visualization of its representativeness in studies published over 40 years to obtain the anatomical characteristics of fossil woods and define their systematic affinities as a specific typology of research data in geosciences. Methods: The methodology involved interviewing a paleobotanical specialist and using different techniques applied in metric studies to map scientific production. Thus, a dataset of (20) thin slide sections of petrified fossil wood used in “Stressing environmental conditions in the “petrified forest” from the Mata Sequence in the Triassic context of the Paraná Basin,” a paper published by the Journal of South American Earth Sciences (DOI:10.1016/j.jsames.2023.104415), was used. Depending on the methodology, these thin petrified wood sheets can reveal paleoclimatic signatures based on the anatomical characteristics of the fossil wood. In addition to this case study, which represents a collection of more than (2.000) thousand blades of fossil wood and approximately 40 years of research, the paleobotanical collection of the department is reused in methodology classes. The collection comprises a database created by research physicists that provides information on anatomical features, systematic affinities, paleoclimatic conditions, and paleoenvironmental insights. Potential for reuse: The reuse, registration, storage, identification, and preservation of thin slide sections as a type of research data used in paleobotany aims to improve the methodology associated with the organization of the physical database of the institution. The research data and blades of fossil wood are being digitized, and soon will be available under the CC BY 4.0 license in the ZENODO repository, according to the sample described here in this data paper (DOI Mendeley:10.17632/b8phfcsync.1). Furthermore, the data can be reused in optical and electronic scanning microscopy software.

Using disposable food packaging materials as printing, embedding, and sectioning media in the plant anatomy lab.

During the COVID-19 pandemic lockdown, all laboratory work was suspended, and we were obliged to work from home, causing delays in our research. As the disruption to supply chains made it difficult to obtain our regular lab supplies, we were obliged to search for substitutes. We became familiar with a plastic material known as biaxially oriented polypropylene (BOPP) that is widely used in the food industry for wrapping or storing fruits, vegetables, and meat. BOPP is easily dissolved in organic solvents such as xylenes, acetone, or thinner, but these reagents are very toxic, flammable, and can cause nausea in some users. After testing several alternatives, we found a polyurethane remover that proved to be an effective and relatively harmless BOPP solvent. By dissolving thin slices of BOPP in a polyurethane solvent, we obtained a clean fluid that we used to obtain leaf surface prints that could be mounted on microscope slides with a coverslip. This fluid produced excellent bark and wood sections and can be used to obtain wood or charcoal surface prints. Our attempts to use it as a mounting medium were unsuccessful. BOPP dissolved in a polyurethane remover is a handy, versatile resource for plant microtechniques. In addition to its economic advantages, it is useful in terms of reducing plastic pollution.

Experimental evaluation of combustion performance in the three main anatomical sections of poplar wood

ABSTRACT The interrelationship and organization between the axial and transversal systems gives rise to three main views, namely cross, radial and tangential sections. This study investigated the differences in combustion properties of the three main anatomical sections of poplar wood and explains the reasons for the differences regarding wood microstructure, pore structure, heat conduction, and microstructure after heat treatment. The thermal conductivity of poplar wood in the cross-section (Wood-CS) was 0.1864 W/m. K, exceeding those of the radial (Wood-RS) and tangential sections (Wood-TS) by 47% and 38%, respectively. In the cone calorimeter test (CC), the peak heat-release rate (pHRR) of Wood-CS samples was 92% and 73% higher than those of the Wood-RS and Wood-TS samples, respectively. This is attributed to the differences in microstructures and the occurrence of macropores in different anatomical sections. This study explored the combustion performance and mechanisms of wood samples in the three main sections, providing crucial theoretical insights for flame retardant impregnation levels in treating wood and aiding in the selection of fire-resistant wood-based building materials.

Anatomical Study for Wood Identification of Shorea Species in Sri Lanka

Wood anatomy is a rapid method for identifying different timber species. Shorea is a diverse genus of trees in the subfamily Dipterocarpaceae and encompasses a group of some 470 tree species that dominate much of the tropical forests of South and Southeast Asia. Many of these are valued commercially for their hardwoods. However, the anatomical identification of Shorea sp. in Sri Lanka has not been fully studied yet. Six Shorea species available in the lowland wet agroecological zone in Ratnapura District, in Sri Lanka, were selected for this study. The study was conducted in the wood Science laboratory of the State Timber Corporation, Sri Lanka. Six Shorea species, including Beraliya dun (S. disticha), Navada dun (S. stupularis), Dun (S. zeylanica), Yakahalu dun (S. trapezifolia), Yakal dun (S. astylosa) and Thiniya dun (S. congestiflora) were used to identify the variation of wood anatomical features to differentiate the species. As a reference, the Publication List of the International Association of Wood Anatomists on anatomical characteristics was used. To determine the microscopic features, six wood sections (Radial, Tangential, and Transverse sections of the wood) representing each species were employed. The anatomical characteristics of the timber were examined using Micrometrics SE Premium 4 software. According to the results, Yakahal Dun species showed the highest number of vessels and Navada represented the lowest number of vessels. The highest value for the vessel diameter showed the Thiniya species and the lowest for the Dun. Beraliya species showed the highest no of rays and the lowest number of rays showed in Yakahalu dun. A Dichotomous key was developed for the exact identification of Shorea species based on the anatomical characteristics.
 
 Keywords: Dichotomous key, IAWA features, Identification, Shore sp., Wood anatomy

Исследование структуры и механических свойств годичных колец древесины дуба черешчатого (Quercus robur) методами наноиндентирования и скретч-теста

Optical methods and optical properties are usually used to research the structure of wood and its ring structure. However, these properties are not directly related to its mechanical and other physical characteristics. To study them, methods of x-ray densitometry, synchrotron radiation, nuclear magnetic resonance, etc., which are not very common in wood science, are used. These methods are quite labor-intensive and require expensive equipment. In this regard, there is a need to develop simple and convenient means and methods for studying the micromechanical properties of wood. The main goal of the work is to develop such an approach using nanoindentation and digital scratching of a cross section of wood and to identify its potential in the further development of dendrochronology and related disciplines. Using the NI method, radial dependences of hardness H and Young's modulus E were obtained for eleven consecutive annual pedunculate oak (Quercus robur L.) wood rings for 3 different loads Pmax = 2, 100 and 500 mN. The values of H in the range from 70 to 340 MPa and Young's modulus E in the range from 2 to 10 GPa were determined for the corresponding loads and early (EW) and late wood (LW). Using the scratch test method, profiles of the normal force Fn and the corresponding hardness HS (in the range from 53 to 225 MPa) were obtained for the period 2007-2020. According to both methods, the widths of annual rings were determined; the discrepancy between the values and the optical method was < 3 %.

Study on the image of fragrant plants in Wood section of Compendium of Materia Medica

As a representative work of materia medica in the Ming Dynasty, the plant images attached to Compendium of Materia Medica retain the results of Li Shizhen's research on the original plants of medicinal materials in another form, which truly reflects the understanding and utilization of herbs in the Ming Dynasty.Taking the image of fragrant plants in Wood section in the book as an example, the original of the plants depicted in the images is examined to reveal the level of understanding of plant knowledge and medicinal properties in the Ming dynasty.Among the 25 images contained in the woody section, 20 images accurately depict the leaves, flowers and fruits of plants, and the description of the morphological details of the plant Cephalotaxus fortunei Hook. f. proves a high level of understanding of plant morphology and medicinal properties at the time. However, the errors of a total of 5 diagrams such as Wuyao and A wei also showed limitations. By comparing the results of the image with the medicinal properties contained in the text,it is clear that the level of understanding of medicinal properties is very high in the Ming dynasty, and the understanding of the medicinal properties of some plants is even higher than the understanding of their morphology.

Anisotropic structural carbon skeleton decorated with Co nanoparticles towards oxygen evolution reaction

Hierarchical carbon skeletons derived from natural wood can be employed as the substrates to fabricate self-supported catalysts. The typically three sections (cross section, radial section, and tangential section) of wood would significantly affect the electrocatalytic performance. Therefore, the correlations between anisotropy structure of wood derived hierarchical carbon skeletons and oxygen evolution reaction (OER) performances was systematically investigated. The cross-sectional wood-derived carbon decorated with Co nanoparticles requires an overpotential of 370 mV to deliver 50 mA cm−2 superior to those of radial-sectional and tangential-sectional counterparts. The cross-section of wood derived carbon skeleton possesses higher content of Co nanoparticles, which is accounted for the enhanced OER catalytic activity. This work highlights the critical contribution of anisotropy of carbon skeletons in promoting the OER properties and provides a new paradigm for designing advanced electrocatalysts.

First Report of Diplodia intermedia Causing Canker and Dieback Diseases on Apple Trees in Canada.

A dieback of apple trees (Malus domestica (Suckow) Borkh.) associated with cankers was observed in commercial orchards in southwestern Ontario, Canada, in 2019. Fifteen 2 to 10-year-old symptomatic trees were collected from three orchards exhibiting up to 37% disease incidence. Small sections of diseased wood (1 cm long) were surface sterilized with 70% ethanol for 30 sec and 1% NaClO for 20 min, rinsed thrice in sterile water, placed on 2% PDA (Difco) amended with kanamycin (50 mg liter-1), and incubated at 22°C for 5 days in the dark (Ilyukhin et al. 2023). Fungal colonies that were consistently isolated were hyphal-tipped, transferred to individual PDA plates and incubated at 22°C for 7 days in the dark. Purified isolates with same characteristics were classed into morphotypes. One morphotype was initially white and turned dark olivaceous with dense aerial mycelium. Pycnidia were produced on pine needles on PDA (Fig. S2) after incubation at 22°C for 17 days in the dark. Conidia were brown, aseptate, ovoid, and measured 27.9 to 31.3 μm x 12.1 to 14.2 μm (mean ± S.D. of 15 conidia = 29.9 ± 0.9 μm × 13.2 ± 0.6 μm), the typical morphology of a Diplodia sp. (Phillips et al. 2012). Genomic DNA was extracted from a 7-day-old culture of a representative isolate M45-28, using the Plant/Fungi DNA Isolation Kit (Norgen Biotech, Canada). The internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α) and β-tubulin gene regions were amplified and sequenced with primers ITS1/ITS4, EF1-728F/EF1-986R and Bt2a/Bt2b and deposited in GenBank with accession numbers MZ970605, MZ995430 and MZ995431, respectively. Based on the sequence, the fungus was identified as Diplodia intermedia A.J.L. Phillips et al. and matched isolates from different hosts and countries (ITS: 100%, MG220378; EF1-α: 100%, MG220385; β-tubulin: 99.24%, MT592502). The maximum likelihood-based phylogenetic analysis of ITS, EF1-α and β-tubulin concatenated sequences was performed using IQ-Tree 2.2.2.7 (Minh et al. 2020). M45-28 was clustered with high bootstrap support values with D. intermedia isolates from the Westerdijk Fungal Biodiversity Institute collection, including the ex-holotype (CBS 124462) (Fig. S1). A living culture of M45-28 was deposited in the Canadian Collection of Fungal Cultures (DAOMC 252253). Pathogenicity assay was conducted by inoculating mycelial plugs from a 7-day-old culture of M45-28 into wounds made on the trunk of 5 eight-month-old potted healthy 'Royal Gala' apple seedlings. Five control seedlings were inoculated with sterile plugs. Canker symptoms appeared 15 days after inoculation, spread around, up and down the main stem from the inoculation point, and by 7 weeks the upper portion of the seedling was dead (Fig. S2). Diplodia intermedia was re-isolated from all inoculated seedlings and species identity was confirmed by sequencing as described above, fulfilling Koch's postulates. Control seedlings remained symptomless and the fungus was not isolated from the wood. Diplodia intermedia was reported to cause cankers on apple in Uruguay (Delgado-Cerrone et al. 2016), wild apple (Malus sylvestris) in Portugal (Phillips et al. 2012), grapevines in France (Comont et al. 2016) and forest trees in Iran (Kazemzadeh Chakusary et al. 2019). To the best of our knowledge, this is the first report of D. intermedia causing canker and dieback diseases on apple trees in Canada. Further studies are required to better understand the epidemiology involved in the dynamic spread of the disease in order to recommend an adequate phytosanitary program for its control.

First Report of Neofusicoccum ribis Causing Cankers and Dieback Diseases on Apricot Trees in Canada and Worldwide.

Apricot trees (Prunus armeniaca L.) with cankers, gummosi and dieback symptoms were observed in a commercial orchard in Niagara-on-the-Lake, Ontario, Canada. In October 2018, up to 44.9% disease incidence (n = 318) was observed on 2-year-old 'Harostar™' trees grafted onto 'Haggith' rootstocks. Fungal colonies were consistently isolated and purified from small sections of wood collected from canker margins of symptomatic trunk and shoot tissue, as described by Ilyukhin et al. (2023). Purified mycelial isolates sharing similar morphological characteristics were categorized into five distinct morphotypes. One representative isolate from each morphotype was used to inoculate excised apricot shoots as described by Ilyukhin and Ellouze (2023). One morphotype displayed necrotic lesions on the shoots consistently yielded abundant white aerial mycelium that turned grey-brown on PDA after 7 days (Figure S1) and produced black pycnidia three weeks following incubtion at 22°C in the dark. Conidia were hyaline, one-celled, fusiform, with dimensions of 19.7 - 24.2 × 3.6 - 4.8 μm (average 22.1 × 4.3 μm, n = 30), the typical morphology of a Neofusicoccum sp. (Crous et al. 2006). Species identification was verified by extracting genomic DNA of the representative isolate M1-105, amplifying and sequencing the internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α) and β-tubulin (TUB2) gene regions with primers ITS1/ITS4, EF1-728F/EF1-986R and Bt2a/Bt2b. Nucleotide sequences (GenBank Accession No. ITS: OK287034; EF1-α: OK346636; TUB2: OK346633) have 100%, 99.61% and 99.55% identity with Neofusicoccum ribis isolates from different hosts and countries (MT587514, DQ235142, OL455952, respectively). Randomized accelerated maximum likelihood analysis (Stamatakis et al. 2008), using ITS, EF1-α and TUB2 sequence data, clustered M1-105 with the highest bootstrap support values with the N. ribis ex-epitype CBS 115475 (Figure S2). A living culture of M1-105 was deposited in the Canadian Collection of Fungal Cultures (DAOMC 252247). Pathogenicity was verified using 5 potted healthy 1-year-old 'Haroblush™' apricot cultivar grafted onto 'Krymsk® 86' rootstocks. Trunks and shoots were inoculated in a shallow wound made by a scalpel with mycelial plugs from a 5-day-old culture of M1-105. Five control trees were inoculated with sterile plugs. Trees were put in an open-air area and watered as needed. Canker symptoms appeared 7 days after inoculation, and spread above and below the inoculation point. Fifteen days post-inoculation, the upper portion of inoculated shoots showed necrosis, gummosis and wilt (Fig. S1). Neofusicoccum ribis was re-isolated from all infected trees and species identity was confirmed by sequencing as described above. Controls remained symptom-free and no fungi were isolated from the wood. Therefore, Koch's postulates were completed. Neofusicoccum ribis was reported to cause branch dieback of olive trees in Spain (Romero et al. 2005) and pistachio in Italy (Corazza et al. 1986), stem blight and dieback of blueberry in Michigan (Heger et al. 2023) and Florida (Wright and Harmon 2010) and postharvest decay of apple fruit from cold storage in Pennsylvania (Jurick et al. 2013). To the best of our knowledge, this is the first report of N. ribis causing canker and shoot dieback of apricot trees in Canada and worldwide. This report reveals N. ribis as a potential threat, causing canker and dieback. Without proper management, it could lead to significant losses in apricot orchards and the stone fruit industry. This study paves the way for crucial research on N. ribis outbreaks and effective disease control.

Optical and mechanical properties of multilayered transparent wood

The versatility of utilizing transparent wood composite (TWC) as structural building component has been envisioned by producing thicker section. The challenges and limitation while preparing transparent wood using thick wood sections can be addressed by the concept of multilayered construction. TWCs were produced using 2 mm thick lignin modified wood veneer sections of three wood species viz., Populus deltoides (Poplar), Melia dubia (Melia) and Grevillea robusta (Silver oak) followed by infiltration of epoxy polymer. To increase the thickness of composite, an easy method of multilayer construction of 2 mm thick epoxy infiltrated wood samples to produce thicker TWC composite was adopted. Assembly of 2 mm thick epoxy infiltrated wood samples one above the other to produce two layer (4 mm thickness), three layer (6 mm thickness) and four layer (8 mm thickness) thicker TWC of Poplar, Melia and Silver oak wood was carried out. The optical transmittance of TWC samples was measured using UV–VIS–NIR spectrophotometer equipped with an integrating sphere. The optical transmittance of multilayered TWC was reduced on increasing number of layers. The three-point static bending test of multilayer TWC indicated an improvement in flexural strength and slight decrease in the modulus of elasticity on increasing number of layers.

Comparison of nail-holding performance of Pinus massoniana and Cunninghamia lanceolata dimension lumber based on round steel nails

In this study, the influence of the diameter of round steel nails, the guiding bores, and the wood sections on the nail holding performance of Pinus massoniana and Cunninghamia lanceolata dimension lumber was explored. The results showed that the nail-holding power of round steel nails mainly came from their friction with wood fibers, while the radial and tangential sections were also affected by the shearing action of wood fibers. The tangential section reached the largest nail-holding power, followed by the radial section and cross section. Greater wood density was associated with higher nail holding power. Under a large nail diameter, however, high-density wood was prone to plastic cracking, which influenced the nail holding power greatly. Prefabricated guiding bores could prevent plastic cracking in wood to some extent and improve the nail holding power of Pinus massoniana and Cunninghamia lanceolata dimension lumber when diameter of round steel nails was more than 3.0 mm. For Cunninghamia lanceolata characterized by low density and rigidity, the wood fiber was in close contact with the round steel nail and internal cracking could not be easily generated under a large diameter of round steel nails.

Minimum invasive sampling method using hollow punch for tropical wood identification on Indonesian cultural heritage

The collection of wood samples is an important step, especially for the identification of wood in cultural heritage. Furthermore, in wood sampling, it is preferable to avoid any damage to minimize destruction to the objects. This study aimed to observe the effectiveness of the minimally invasive sampling method using a hollow punch for wood identification on wooden cultural heritage, such as the Panji mask (Java).The materials used as a testing model were three tropical wood species. Sampling was conducted with various diameters of hollow punch at transverse, tangential, and radial sections. The anatomical structures were compared to the features according to the IAWA list of microscopic features for hardwood identification. The result showed that the most effective method was to take samples using a 1.5 mm diameter of hollow punch at the tangential section of wood.