Wood-derived Biochar Research Articles

Sustainable enhancement of biogas production from a cold-region municipal wastewater anaerobic digestion process using optimized sludge-derived and commercial biochar additives

Anaerobic digestion (AD) of municipal wastewater sludges produces valuable solid digestate and biogas. Biogas is a source of clean energy and enhancement of its production has been of recent interest for increased electricity generation, among other products. The objective of this study was the development of a novel municipal sludge-derived biochar and its application in a municipal wastewater AD system to increase the biogas production rate. Thickened waste-activated sludge (TWAS) samples were collected from the cold-region municipal wastewater treatment plant and used to synthesize biochar applied in the simulation of AD processes using laboratory-scale reactors. The TWAS-derived biochar was synthesized using the commonly used furnace pyrolysis (sludge-based biochar, SBC), and more novel microwave pyrolysis including phosphoric acid as a microwave activator (activated sludge-based biochar, ASBC). The microwave pyrolysis conditions were optimized using a computational fluid dynamics (CFD) technique. In addition, various commercially available carbon-based additives were assessed for their impacts on the AD process including activated carbon, wood-derived biochar, and forest residue-derived biochar. Results showed that the ASBC increased the cumulative methane production by 50% (333 mL/g VS) versus the control sample (221 mL/g VS) after 30 d. The ASBC showed higher surface area, electrical conductivity, and metal contents versus the other biochars which boosted the AD microbial community growth leading to higher organic matter conversion into biogas. In the ASBC-amended digesters, the bacterial phylum Bacteroidota, which contains a major genus of the dgA-11-gut-group, exhibited a synergy between organic substrate fermentation and volatile fatty acid production, resulting in enhanced biogas production. The TWAS biochar demonstrated promising performance in enhancing the AD process fostering energy and resource self-sufficiency at municipal wastewater treatment plants. This smart sludge management aligns well with sustainable waste management practices and clean energy production strategies, especially considering that the biochar was sourced from a readily available continuous waste-product stream.

Influence of composted manures and co-composted biochar on growth performance of saffron and soil nutrients under varying electrical conductivity soil conditions: A two-year field study

The stigmas of saffron (Crocus sativus L.) are known as “red gold” for being the most expensive spice of the world. This medicinal crop grows well in the regions with cold winter Mediterranean climate. However, salinity can be one of the limiting factors for its cultivation in these regions. Composted manures and their co-compost with wood-derived biochar as fertilizers in saline soil can reduce the salinity stress on saffron. In this study, the composted manures from dairy farms (FYM), sheep and goats (SG) and poultry (PM) and their co-compost with wood-derived biochar (FYM-B, SG-B and PM-B) were amended in non-saline (EC1:2 0.25 dS m−1, SOM; 9.9 g kg−1) and soil with natural slight salinity (EC1:2; 1.95 dS m−1, SOM; 30.9 g kg−1) soil for two consecutive years with the total amendment rate of 65 t ha−1 for each fertilizer. The saline soil was transported from an agricultural farm (30 km away from research field); whereas, the non-saline soil was not transported and was not cultivated before. The amendment of fertilizers and sowing of corms were conducted at the same time in the mid of August. The stigma yield in saline control soil was significantly lower than non-saline control in both cropping years. In non-saline soil, all fertilizers reduced stigma yield of first-year crop by 14–60 % (P < 0.05). No differences between treatments for the stigma yield of second year crop in non-saline and of first year crop in saline soil were observed. In saline soil, FYM and PM fertilizers increased stigma yield of second year crop by 44 % and 41 %, respectively (P < 0.05). The two-year cumulative agronomic efficiencies for nitrogen and phosphorus and the nitrogen use efficiency for leaf biomass were lower under fertilizer treatments than control in non-saline and saline soil. In non-saline soil the PM-B; whereas, in saline soil all fertilizers (except SG) increased the phosphorus use efficiency of leaves (P < 0.05). The two-year amendment of composted manures and FYM-B in non-saline soil resulted in high uptake of sodium in leaves (P < 0.05); whereas, such effect was not observed for saline soil. Almost all fertilizers increased ∼2–5 fold the concentration of bioavailable phosphorus and ∼2–3 fold the concentration of potassium in non-saline and saline soil (P < 0.05). The positive influence of fertilizers on stigma yield and PUE of leaves of second year crop was observed only for saline soil. Furthermore, contrary to the results for non-saline soil, these fertilizers did not cause an increased uptake of Na in leaves in saline soil. We attribute this effect to the high concentration of SOM and gradual decline in EC (0.94–1.71 dS m−1) over time in saline soil. The gradual improvement in yield (from negative to neutral in non-saline and from neutral to positive in saline soil) was observed over time. Amendment of fertilizers and sowing of corms at same time may be the reason for their negative influence on stigma yield in low SOM-containing non-saline soil.

Designing a biochar-based pretreatment method for distillery effluents entering constructed wetlands

Spent lees is an important distillation waste by-product created during whisky production, characterised by low pH and high dissolved copper (dissCu) content. Constructed wetland systems (CWs) are used in some Scotch whisky distilleries to treat spent lees prior to surface water discharge to prevent substantial environmental risks of spent lees in aquatic ecosystems, by buffering the acidic nature and decreasing the dissCu and organics content. However, as these wetland systems are biological communities (containing bacteria, fungi, plants, etc.), direct disposal of spent lees into CW ecosystems presents a challenge given its nature. This study investigates the efficacy of modified biochar (waste) sourced from whisky cask cooperage operations, as an effluent pretreatment method. The aim is to transform this raw biochar into NaOH-modified biochar, which will be capable of buffering the effluent pH and removing dissCu from spent lees before it enters CWs. To enhance raw biochar, NaOH modification and additional heat treatment were utilised which increased the alkalinity, heavy metal removal capacity and pH buffering potential. Experiments with 0.2% and 2% NaOH treated biochar demonstrated its treatment performance using a diverse range of spent lees samples with varying characteristics, including pH (4.1–4.4), dissCu concentrations (33.8–59.5 mg/L), and total organic carbon (685–754 mg/L). Column studies further supported the efficacy of the modified biochars, showing significant pH elevation and efficient dissCu removal (of 5.72 mg of Cu per g of biochar) when treating spent lees. This dissCu removal capacity compares favorably with other wood-derived biochars studied in the literature, which have reported between 2.75 and 7.44 mg/g. Surface characterisation techniques (EDX, XPS, FTIR, XRD) showed how the Cu ions microprecipitated on the modified biochar surface, validating its remediation potential. This study highlights the potential of biochar as a sustainable pretreatment solution for distillery effluents, paving the way for enhanced and more circular waste management practices within the Scotch whisky industry.

Enhanced lipid production of Auxenochlorella pyrenoidosa using biochar-pretreated tobacco industry wastewater

Tobacco industry wastewater, containing high levels of nicotine, organic carbon, dyes, and other pollutants, presents significant environmental and health challenges. This study evaluates the combined application of wood-derived biochar and microalgal biorefineries for effective treatment of this wastewater. A 3 % biochar dosage was determined to be optimal, achieving complete nicotine removal, enhancing wastewater transmittance from 12.29 % to 88.8 %, and reducing chemical oxygen demand (COD) levels by 87.7 % to 1757.3 mg/L, creating conditions favorable for the growth of Auxenochlorella pyrenoidosa. The pretreatment preserved key nutrients, such as sugars, total nitrogen (TN), and total phosphorus (TP), which supported enhanced microalgal photosynthesis and led to significant increases in biomass and lipid accumulation, particularly in unsaturated fatty acids. This shift in lipid composition contributed to a significant reduction in the cold filter plugging point value, improving the suitability of microalgae-derived biodiesel for use in cold climates. Transcriptomic analysis revealed upregulation of pathways involved in photosynthesis, carbon fixation, and fatty acid biosynthesis, while downregulation of the tricarboxylic acid (TCA) cycle minimized acetyl-CoA consumption, further promoting lipid synthesis. This integrated approach not only effectively detoxifies tobacco industry wastewater but also converts it into valuable biofuels, offering a promising and sustainable strategy for waste management and biofuel production.

Optimizing native vegetation establishment in urban soils: Assessing the impacts of organic amendments on specific growth parameters

Following soil disturbances, establishing healthy roadside vegetation can reduce surface water runoff, improve soil quality, decrease erosion, and enhance landscape aesthetics. This study explores the use of organic soil amendments (OAs) as alternatives to conventional vegetation growth approaches, aiming to provide optimal compost mixing ratios for poor soils, and clarify guidelines for OAs’ use in roadside projects. Three sandy loam soils and one loam soil were chosen for the study. Organic amendments included yard waste (Y), food waste (F), turkey litter and green waste-based (T) composts, and wood-derived biochar (B). Treatment applications targeted specific increases in the organic matter (OM) percentage of the soils. A selection of seven native species (grasses and forbs) in a total of 156 pots (4 control soils + 4 soils x 4 OAs x 3 application rates, all prepared in triplicates) was used for the pot study experiment. A significant correlation between electrical conductivity (soluble salts) in soil-OA blends and corresponding percent green coverage (%GC) was found. High salts from the T compost either delayed or curtailed growth. Notably, 3 out of the 4 soils amended with biochar exhibited rapid vegetation coverage during initial growth stages compared to other soil-OA blends but reduced the nitrogen (N) uptake and leaf area in black-eyed Susan (BES) plants. In contrast, N uptake was higher in the BES plants emerging from composts T, F, and Y compared to biochar. It is recommended to minimize concentrated manure-based (e.g., turkey litter) composts for roadside projects as an OM source, and alternatively, enriching wood-based biochar with nutrients when used as a soil amendment. Within the current study, composts such as F and Y were well-suited to establish healthy and long-lasting vegetation.

Wood-derived biochar as a matrix for cost-effective and high-performing composite thermal energy storage materials

Wood-derived biochar as a matrix for cost-effective and high-performing composite thermal energy storage materials

Importance of pyrolysis temperature and particle size on physicochemical and adsorptive properties of urban wood-derived biochar

Physicochemical properties of biochars made from urban wood waste at different pyrolysis temperatures (400-700 °C) and particle sizes (1-2 mm and 63-75 μm) were compared to their ability for heavy metals adsorption at various contact times (30-300 min) and adsorbent dosages (0.1-5 g L-1) in real landfill leachate. The yield, volatile matter, hydrogen and oxygen contents, and functional groups of biochars decreased with increasing pyrolysis temperature; while pH, electrical conductivity, ash, fixed carbon, and specific surface area (SSA) increased. FE-SEM images showed that the creation of pores on the surface of biochars increased significantly with increasing pyrolysis temperature. Moreover, particle size exhibited no significant effect on the properties of biochars (p>0.05), except for the SSA. The SSA of biochars with a particle size of 63-75 μm was significantly higher than that measured in 1-2 mm biochars. The adsorption efficiency of heavy metals from landfill leachate increases with increasing pyrolysis temperature, contact time and adsorbent dosage. Conversely, reducing the particle size increases the adsorption efficiency. The highest adsorption efficiency for Pb, Zn, Ni, Cd, and Mn was obtained as a result of using biochar pyrolyzed at 700 °C with a particle size of 63-75 μm (i.e., BC700/63-75) by 60.39%, 75.01, 64.36, 58.87%, and 71.15%, respectively. Pseudo second-order kinetic and the Langmuir isotherm were recognized as the most appropriate models to describe the adsorption process. The chemical adsorption was identified as the main mechanism for the adsorption of metal ions onto BC700/63-75.

Phytotoxicity and hormesis in common mobile organic compounds in leachates of wood-derived biochars

AbstractAlthough addition of pyrolyzed organic materials (biochars) to soil generally results in increased growth and physiological performance of plants, neutral and negative responses have also commonly been detected. Toxicity of organic compounds generated during pyrolysis, sorbed by biochars, and then released into the soil solution, has been implicated as a possible mechanism for such negative effects. Conversely, water-soluble biochar constituents have also been suggested to have “hormetic” effects (positive effects on plants at low concentrations); however, no specific compounds responsible have been identified. We investigated the relative phytotoxicity—and possible hormetic effects—of 14 organic compounds common in aqueous extracts of freshly produced lignocellulosic biochars, using seed germination bioassays. Of the compounds examined, volatile fatty acids (VFAs: acetic, propionic, butyric, valeric, caproic, and 2-ethylbutyric acids) and phenol, showed acute phytotoxicity, with germination-based ED50 values of 1–30 mmol L−1, and 2-ethylbutyric acid showed ED50 values of 0.1–1.0 mmol L−1. Other compounds (benzene, benzoic acid, butanone, methyl salicylate, toluene, and 2,4-di-tert-butylphenol) showed toxic effects only at high concentrations close to solubility limits. Although phytotoxic at high concentrations, valeric and caproic acid also showed detectable hormetic effects on seedlings, increasing radicle extension by 5–15% at concentrations of ~ 0.01–0.1 mmol L−1. These data support the hypothesis that VFAs are the main agents responsible for phytotoxic effects of lignocellulosic biochar leachates, but that certain VFAs also have hormetic effects at low concentrations and may contribute to positive effects of biochar leachates on early plant development in some cases. Graphical Abstract

Biochar-based polymeric film as sustainable and efficient sorptive phase for preconcentration of steroid hormones in environmental waters and wastewaters

BackgroundThe environmental impact of sample preparation should be minimized through simplification of the procedures and the use of natural, renewable and/or reusable materials. In such scenario, thin-film microextraction fulfils the former criteria, as it enables few steps and miniaturization, thus small amount of extraction phase. At the same time, the use of sorbents such as biochars obtained from biomass waste is even more promoted due to their availability at low cost and increased life-cycle in a circular economy vision. However, it is not always easy to combine these criteria in sample preparation. ResultsA thin film microextraction was developed for the determination of steroids in aqueous samples, entailing a membrane made of cellulose triacetate and a wood-derived biochar (Nuchar®) as carbon precursor. Different characterization techniques showed the successful preparation, whereas the sorption kinetics experiments demonstrated that biochar is responsible for the extraction with the polymer acting as a smart support. After a study about membranes’ composition in terms of biochar amounts (4 %, 10 %, 16 % wt) and type of synthesis set up, the ceramic 3D-mold was selected, achieving reproducible and ready-to-use membranes with composition fixed as 10 %. Different elution conditions, viz. type and time of agitation, type, composition and volume of eluent, were evaluated. The final microextraction followed by HPLC-MS/MS quantification was successfully validated in river and wastewater treatment plant effluent samples in terms of accuracy (R% 64–123 %, RSD<19 % in river; R% 61–118 %, RSD <18 % in effluent, n = 4), sensitivity (MQLs 0.2–8.5 ng L−1) and robustness. SignificanceThis novel biochar-based polymeric film proved to be a valid and sustainable sorbent, in terms of extraction capability, ease of preparation and greenness. By comparison with literature and the greenness evaluation with the most recent metric tools, this method expands the potential applicability of the thin-film microextraction and opens up innovative scenarios for sustainable procedures entailing the use of biochars entrapped in bio-polymers.

Accumulation and Release of Cadmium Ions in the Lichen Evernia prunastri (L.) Ach. and Wood-Derived Biochar: Implication for the Use of Biochar for Environmental Biomonitoring.

Biochar (BC) boasts diverse environmental applications. However, its potential for environmental biomonitoring has, surprisingly, remained largely unexplored. This study presents a preliminary analysis of BC's potential as a biomonitor for the environmental availability of ionic Cd, utilizing the lichen Evernia prunastri (L.) Ach. as a reference organism. For this purpose, the lichen E. prunastri and two types of wood-derived biochar, biochar 1 (BC1) and biochar 2 (BC2), obtained from two anonymous producers, were investigated for their ability to accumulate, or sequester and subsequently release, Cd when exposed to Cd-depleted conditions. Samples of lichen and biochar (fractions between 2 and 4 mm) were soaked for 1 h in a solution containing deionized water (control), 10 µM, and 100 µM Cd2+ (accumulation phase). Then, 50% of the treated samples were soaked for 24 h in deionized water (depuration phase). The lichen showed a very good ability to adsorb ionic Cd, higher than the two biochar samples (more than 46.5%), and a weak ability to release the metal (ca. 6%). As compared to the lichen, BC2 showed a lower capacity for Cd accumulation (-48%) and release (ca. 3%). BC1, on the other hand, showed a slightly higher Cd accumulation capacity than BC2 (+3.6%), but a release capacity similar to that of the lichen (ca. 5%). The surface area and the cation exchange capacity of the organism and the tested materials seem to play a key role in their ability to accumulate and sequester Cd, respectively. This study suggests the potential use of BC as a (bio)monitor for the presence of PTEs in atmospheric depositions and, perhaps, water bodies.

Developing Prussian blue/wood-derived biochar catalyst for persistent organic pollutant degradation: Preparation, characterization, and mechanism

Biomass-derived biochar shows broad promise for persistent organic pollutants (POPs) degradation and thus establishes a more sustainable homestead. However, effective catalytic performance is still challenging. Herein, an efficient catalyst (Prussian blue decorated wood-derived biochar, PBB) was constructed by introducing Prussian blue (PB) into wood-based biochar to activate peroxymonosulfate (PMS) for removing POPs. After anchoring of PB, the degradation performance of biochar was enhanced (degradation efficiency of methylene blue (MB, 20 mg/L) increased from 52% of biochar to 95% of PBB within 60 min). The PBB presents effective MB degradation performance with a wide pH value (3.0 < pH < 11.0) or co-existing diverse anions (Cl−, NO3−, H2PO4−, and HCO3−). Electron paramagnetic resonance (EPR) analysis as well as electrochemical tests confirmed that the non-radical pathway (1O2) is the key to biochar activation of PMS, but by restricting PB into the biochar, the radical pathway (SO4•- and •OH), the non-radical pathway (1O2), and direct electron transfer can work together to activate PMS. In addition, the degradation efficiency could remain about 80% after five-time cyclic tests. This work elucidates the role of PB nanoparticles in enhancing biochar catalysts, which can inspire the development of a carbon-neutralized, cost-effective, and effective strategy for POPs removal.

Biochar-based organic fertilizers: Influence on yield and concentration of antioxidants in the stigma of saffron and rhizosphere bacterial diversity of slightly saline and non-saline soils

Being the most expensive spice, saffron has great economic importance. This crop grows well in cold arid deserts. Salinity is one of the important limiting factors for the cultivation of this crop. However, the use of composted manured and co-composted biochar and fertilizers can play a role in attenuating the salinity stress on this crop. In this two-year field study, manures from three sources: sheep and goat (SG), cow and buffalo (FYM), and poultry (PM) farms, and their co-compost with slow-pyrolyzed wood-derived biochar (B) were used for saffron cultivation in slightly saline (electrical conductivity 1.95 dS m−1) and non-saline soils. Yield and concentration of antioxidants of stigma and bacterial diversity in the rhizosphere of this crop, under salinity and non-salinity conditions, were evaluated. Results revealed that in non-saline soil of first-year crops, all fertilizers decreased the yield of stigma than control by 15–49 % (P ≤ 0.05) but increased the concentration of carotenoids and total polyphenolics (P ≤ 0.05). In saline soil, no difference in yield was observed between treatments for the first-year crop; however, for the second-year crop, as compared to control, PM and FYM significantly increased yield by 41 % and 44 % respectively, whereas FYM also increased the concentration of total polyphenolics (P ≤ 0.05). The FYM fertilizer was found suitable for the yield and quality of saffron stigma for second-year crops in both soils (non-saline and saline). The observed OTUs, Chao1, Fischer, and ACE indexes based on 16 s rRNA metagenomic analysis revealed 2–4 times greater bacterial diversity in the rhizosphere soil of PM-B and SG-B treatments than in the control. Furthermore, 347 bacterial species were found in PM-B- or SG-B-amended soils absent in control treatments.

Assessing construction and demolition wood-derived biochar for in-situ per- and polyfluoroalkyl substance (PFAS) removal from landfill leachate

With regulations for per-and polyfluoroalkyl substances (PFAS) impending, the abundance of these chemicals of emerging concern in municipal solid waste (MSW) landfill leachate increasingly challenges landfill operators to seek on-site leachate pre-treatment options. This two-staged study explores the potential reuse of biochar derived from construction and demolition debris (CDD) wood as an in-situ PFAS sorbent for application within MSW landfill leachate collection systems. Batch leaching tests were first used to examine the feasibility of capturing PFAS from landfill leachate using two sources of CDD-wood-derived biochar. Then, columns were used to test the in-situ sorption capabilities of the same biochars under simulated landfill conditions. All leachates were characterized for pH, chemical oxygen demand, ammonia-nitrogen, and 92 PFAS. Seventeen PFAS were detected in the batch leaching experiment, and nine PFAS were detected in column leachates. In the batch leaching scenario, Biochar 1 achieved a maximum of 29% PFAS reduction compared to controls. Columns containing Biochar 1 generated leachates with PFAS concentrations 50% to 80% higher than those in control columns for the duration of the experiment. Columns containing Biochar 2 generated leachates with PFAS concentrations 44% less than controls in week 1 and similar concentrations in weeks 2, 3, and 4. In this study, PFAS removal from landfill leachate using biochar derived from CDD wood was not significant. Further research on biochar derived from CDD wood is needed before it can be recommended as an in-situ landfill leachate pre-treatment method.

Pyrogenic Black Carbon Suppresses Microbial Methane Production by Serving as a Terminal Electron Acceptor.

Methane (CH4) is the second most important greenhouse gas, 27 times as potent as CO2 and responsible for >30% of the current anthropogenic warming. Globally, more than half of CH4 is produced microbially through methanogenesis. Pyrogenic black carbon possesses a considerable electron storage capacity (ESC) and can be an electron donor or acceptor for abiotic and microbial redox transformation. Using wood-derived biochar as a model black carbon, we demonstrated that air-oxidized black carbon served as an electron acceptor to support anaerobic oxidation of organic substrates, thereby suppressing CH4 production. Black carbon-respiring bacteria were immediately active and outcompeted methanogens. Significant CH4 did not form until the bioavailable electron-accepting capacity of the biochar was exhausted. An experiment with labeled acetate (13CH3COO-) yielded 1:1 13CH4 and 12CO2 without biochar and predominantly 13CO2 with biochar, indicating that biochar enabled anaerobic acetate oxidation at the expense of methanogenesis. Methanogens were enriched following acetate fermentation but only in the absence of biochar. The electron balance shows that approximately half (∼2.4 mmol/g) of biochar's ESC was utilized by the culture, corresponding to the portion of the ESC > +0.173 V (vs SHE). These results provide a mechanistic basis for quantifying the climate impact of black carbon and developing ESC-based applications to reduce CH4 emissions from biogenic sources.

Understanding a wood-derived biochar's impact on stormwater quality, plant growth, and survivability in bioretention soil mixtures

Bioretention systems are planted media filters used in stormwater infrastructure. Maintaining plant growth and survival is challenging because most designs require significant sand. Conventional bioretention soil media (BSM) might be augmented with biochar to make the BSM more favorable to plants, to improve nutrient removal efficiency, and enhance plant survivability during drought while replacing compost/mulch components that have been linked to excess nutrient export. Pots with BSMs representing high and moderate sand content were amended with wood biochar, planted with switchgrass, and subjected to weekly storms for 20 weeks, followed by a 10-week drought. After 20 weeks, 4% biochar amendment significantly increased stormwater infiltration (67%) and plant available water (52%) in the high sand content BSM (NC mix, which meets requirements for the state of North Carolina (US) and contains no compost/mulch), and these favorable hydraulic properties were not statistically different from a moderate sand content, biochar-free BSM with compost/mulch (DE mix, which meets requirements for state of Delaware (US)). While biochar amendment improved plant height (25%), the number of shoots (89%), and total biomass (70%) in the NC mix, these parameters were still less than those in the biochar-free DE mix containing compost/mulch. TN and NO3−1 removal were also improved (28–35%) by biochar amendment to NC mix, and the resulting TN and TP loadings to groundwater were 10 and 7 times less, respectively than biochar-free DE mix with compost/mulch. During the drought period, biochar amendment increased the time to switchgrass wilting by ∼8 days in the NC mix but remained 40% less than the biochar-free DE mix. A recalcitrant carbon-like biochar mitigates some of the deleterious effects of high sand content BSM on plants, and where nutrient pollution is a concern, replacement of compost/mulch with wood biochar in BSM may be desired.

Improving bifunctional catalytic activity of biochar via in-situ growth of nickel-iron hydroxide as cathodic catalyst for zinc-air batteries

Expanding the application scenarios of wood-derived biochar guided by the conversion of traditional energy to new energy shows great promise as a field. As thrilling energy conversion apparatus, zinc-air batteries (ZABs) require cathode catalysts with high oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) activities and stability. Herein, two-dimensional nickel-iron hydroxide nanosheets were creatively assembled in N-doped wood-derived biochar (NiFe-LDH@NC) by an in-situ growth method. The categorized porous organization in wood-derived biochar facilitates the rapid seepage of electrolytes and rapid diffusion of reaction gases. The unique interfacial structure of biochar and NiFe-LDH accelerates electron transfer during oxygen electrocatalysis, and endows NiFe-LDH@NC with first-class catalytic activity and durability for ORR and OER. The ZAB derived from NiFe-LDH@NC showed elevated discharge productivity and cycle endurance, making it promising for viable applications. This work provided a convenient way for the conversion of wood-derived biochar to high-value added electrocatalysts.Graphical

Biological treatment as a green approach for enhancing electrochemical performance of wood derived carbon based supercapacitor electrodes

Wood-derived biochar is an eco-friendly and porous carbon material that can be used to prepare supercapacitor electrodes. For the purpose of addressing the deficiencies in the specific surface area and pore structure of wood-derived carbon as the raw material of electrodes, a biological pre-treatment method was developed to construct the desirable pore structure of wood by leveraging the lignin-degrading property of white-rot fungi. First, white-rot fungi with permeability and biodegradability on lignocellulosic substrates were used to regulate the pore structure of natural paulownia wood, which was then carbonized to produce a porous hyphae/wood-derived carbon conductive substrate. Afterward, manganese dioxide (MnO2) was deposited on the substrate to fabricate the MnO2/hyphae/wood-derived carbon (HWC-3M) electrode by the hydrothermal method. The degradation of lignin by white-rot fungi broke the original pore structure of wood, strengthened effective channels in the cell walls, and facilitated the loading of MnO2 into the channels to improve the ion transport rate. The HWC-3M had richer energy storage sites, more electrolyte ion transport channels, and better electrochemical performance than the control electrode made of untreated wood-derived carbon (HWC-0), showing an excellent areal specific capacitance (3395 mF/cm2 at a current density of 1.0 mA/cm2), moderate gravimetric specific capacitance (138.3 F/g), and good cycling stability (retention of 88.6% after 2000 cycles). The results indicate that pretreating wood-derived carbon composite electrode with white-rot fungi serves as a new green approach to develop high-performance electrochemical energy storage devices.

Biochar effects on early decomposition of standard litter in a European beech forest (northern Italy)

The release of biochar (BC) on forest soil is a strategy aimed at increasing carbon reserves and forest productivity. The effect of BC amendments on the decomposition of different quality litter is, however, poorly understood. With this study we investigate the effects of wood-derived BC applications on early decomposition in a European beech (Fagus sylvatica L.) forest through the burial of standard material, i.e. green tea and rooibos tea (high- and low-quality litter surrogates, respectively). Two main questions were addressed: 1) Do BC applications influence the decomposition of high- and low-quality standard litter and, if so, in what way? and 2) Does this effect (if measurable) depend on where the sample is placed with respect to the BC application layer? To test BC amendment effects, four application percentages were employed (0, 10, 20 and 100 %), after which standard litter mass loss was recorded. To investigate the effects of sample position, only three BC application percentages were used (0, 10 and 20 %), with teabags buried at three different depths – within the BC amended layer, between this layer and the unamended soil, and below the latter. Results show that early decomposition of high-quality standard litter was not influenced by BC applications, while a significant reduction in mass loss of low-quality standard litter was observed when the percentage of BC application was higher, specifically of litter within the 20 % and 100 % BC amended layers. Decomposition was also affected by sample position relative to the BC layer, exhibiting higher levels of mass loss when samples were placed within the BC amended layer. Overall, BC applications on beech forest soils not only seem to produce negligible effects on the early decomposition rate of high-quality standard litter, but such applications also seem to have the ability to reduce carbon loss following plant material degradation.

Effects of biochar on soil microbial communities: A meta-analysis

Changes in soil microbial communities may impact soil fertility and stability because microbial communities are key to soil functioning by supporting soil ecological quality and agricultural production. The effects of soil amendment with biochar on soil microbial communities are widely documented but studies highlighted a high degree of variability in their responses following biochar application. The multiple conditions under which they were conducted (experimental designs, application rates, soil types, biochar properties) make it difficult to identify general trends. This supports the need to better determine the conditions of biochar production and application that promote soil microbial communities. In this context, we performed the first ever meta-analysis of the biochar effects on soil microbial biomass and diversity (prokaryotes and fungi) based on high-throughput sequencing data. The majority of the 181 selected publications were conducted in China and evaluated the short-term impact (<3 months) of biochar. We demonstrated that a large panel of variables corresponding to biochar properties, soil characteristics, farming practices or experimental conditions, can affect the effects of biochar on soil microbial characteristics. Using a variance partitioning approach, we showed that responses of soil microbial biomass and prokaryotic diversity were highly dependent on biochar properties. They were influenced by pyrolysis temperature, biochar pH, application rate and feedstock type, as wood-derived biochars have particular physico-chemical properties (high C:N ratio, low nutrient content, large pores size) compared to non-wood-derived biochars. Fungal community data was more heterogenous and scarcer than prokaryote data (30 publications). Fungal diversity indices were rather dependent on soil properties: they were higher in medium-textured soils, with low pH but high soil organic carbon. Altogether, this meta-analysis illustrates the need for long-term field studies in European agricultural context for documenting responses of soil microbial communities to biochar application under diverse conditions combining biochar types, soil properties and conditions of use.

N,S-codoped biochar outperformed N-doped biochar on co-activation of H2O2 with trace dissolved Fe(Ⅲ) for enhanced oxidation of organic pollutants

Co-activation of H2O2 with biochar and iron sources together provides an attractive strategy for efficient removal of refractory pollutants, because it can solve the problems of slow Fe(Ⅱ) regeneration in Fenton/Fenton-like processes and of low •OH yield in biochar-activated process. In this study, a wood-derived biochar (WB) was modified by heteroatom doping for the objective of enhancing its reactivity toward co-activation of H2O2. The performance of the co-activated system using doped biochars and trace dissolved Fe(Ⅲ) on oxidation of organic pollutants was evaluated for the first time. The characterizations using X-ray photoelectron spectroscopy (XPS), Raman spectra and electrochemical analyses indicate that heteroatom doping introduced more defects in biochar and improved its electron transfer capacity. The oxidation experiments show that heteroatom doping improved the performance of biochar in the co-activated process, in which the N,S-codoped biochar (NSB) outperformed the N-doped biochar (NB) on oxidation of pollutants. The reaction rate constant (kobs) for oxidation of sulfadiazine in NSB + Fe + H2O2 is 2.25 times that in NB + Fe + H2O2, and is 72.9 times that in the Fenton-like process without biochar, respectively. The mechanism investigations indicate that heteroatom doping enhanced biochar's reactivity on catalyzing the decomposition of H2O2 and on reduction of Fe(Ⅲ) due to the improved electron transfer/donation capacity. In comparison with N-doping, N,S-codoping provided additional electron donor (thiophenic C–S–C) for faster regeneration of Fe(Ⅱ) with less amount of doping reagent used. Furthermore, co-activation with NSB maintained to be efficient at a milder acidic pH than Fenton/Fenton-like processes, and can be used for oxidation of different pollutants and in real water. Therefore, this research provides a novel, sustainable and cost-efficient method for oxidation of refractory pollutants.