Flow modulation mechanism in a cylinder with corrugated surfaces is investigated by wind tunnel experiments at Reynolds number Re=25 600. Experimental results include surface pressure measurement for the cylinder wall and particle image velocimetry (PIV) for the wake flow. A pair of corrugated surfaces are symmetrically installed on the cylinder wall. Corrugated surfaces are distributed at three different locations on the cylinder wall, i.e., the windward part (case 1), leeward part (case 2), and lateral part (case 3). Experimental results show that corrugated surfaces can modify surface pressure, aerodynamic forces, and vorticity evolution of the cylinder flow. Compared with the natural cylinder (baseline case), the mean drag and fluctuating lift forces of case 1 are reduced by 58% and 82%, which are optimal among all test cases. Flow modulation effects of case 2 on global cylinder wake flow are unobvious and that of case 3 are between cases 1 and 2. Corrugated surfaces can also modify modal properties of proper orthogonal decomposition (POD) of the cylinder wake. Moreover, characteristics of recirculation bubbles, velocity deficits, turbulence kinetic energy, and Reynolds stresses in the wake are all modulated. The main flow modulation mechanism is that shear-layer shapes and streamline distributions near the corrugated surfaces are changed based on zoom-in PIV results on the cylinder near-wall region.