Wild Sources Research Articles

Relative abundance of a mesocarnivore in a human-dominated, semi-arid rangeland in Namibia

Mesocarnivores fill important roles in ecological communities globally, but their distribution and abundance are often understudied. Many species have historically been regarded as vermin and subject to lethal control due to their role in livestock predation. Identifying the factors influencing mesocarnivore populations can help disentangle their relationship within ecological communities and inform conflict mitigation and conservation priorities. To help identify these factors, we used camera traps to study the community of medium and large mammals in four communal conservancies of northeastern Namibia covering the wet and dry seasons using 99 and 97 camera trap stations, respectively. We modelled black-backed jackal (Lupulella mesomelas) abundance using the robust Royle-Nichols model. Black-backed jackal were widespread, with a mean per site abundance of 2.01 (SD=0.66) in the wet season and 2.41 (SD=0.49) in the dry season. Black-backed jackal showed seasonally contrasting covariate associations, with lower abundance in areas with medium and large-sized wild prey during the wet season, and higher abundance in areas with more villages and close to African wild dog (Lycaon pictus) dens in the dry season. We identified localized hotspots of black-backed jackal abundance during the dry season, which may indicate that when resources are scarce, black-backed jackals rely on anthropogenic food sources despite an elevated risk for conflict, and on carcass remains from African wild dog kills. These findings highlight potential drivers of mesocarnivore abundance that would be obscured in a conventional occurrence modelling framework, and illustrate how local abundance may be influenced by seasonal variability, wild and anthropogenic food sources, and a likely facilitative relationship with a large carnivore. Further investigations in areas with more complex carnivore guilds and higher density of dominant predators are needed to understand black-backed jackal-African wild dog interactions and impacts on population dynamics.

Ethnobotanical study of medicinal plants in Dibatie district, Metekel zone, Benishangul Gumuz Regional State, western Ethiopia

BackgroundHerbal medicine has been used for the treatment of human and livestock ailments since ancient times. Numerous rural and urban communities in Ethiopia practice traditional medicine and transfer the knowledge verbally from generation to generation. Thus, this study was conducted to document the traditional medicinal plants and associated indigenous knowledge in Dibatie district, Metekel zone, Benishangul Gumuz Regional State, western Ethiopia.MethodsThree hundred seventy-four (374) informants from 11 kebeles (the smallest administrative units) were selected and participated in the data delivery. The ethnobotanical data collection was carried out using semi-structured interviews, preference ranking, direct matrix ranking, field observation, market surveys, and focus group discussions, including voucher specimen collections. The ethnobotanical data were analyzed using descriptive statistics (frequency and percentage), ranking, comparison, and quantitative ethnobotanical techniques such as informant consensus factor, fidelity level index, Jaccard’s coefficient of similarity, and use value index.ResultsA total of 170 plant species were recorded to treat 79 human and 29 livestock ailments. Fabaceae (with 20 species) and Asteraceae (with 18 species) were the most dominant medicinal plant families in the area. Most remedial plants were herbs (61 species, 35.88%), followed by shrubs (39 species, 22.94%). The majority (135 species, 79.41%) of medicinal plants were harvested from wild sources and mainly possessed multiple remedy parts (41.17%) that are usually prescribed in fresh form (60.13%). The most commonly reported human ailment was snake venom, while blackleg was mostly reported among livestock diseases. The herbal medicines were mostly administered orally (52.20%), followed by dermal (17.62%) application. Embelia schimperi Vatke, Glinus lotoides L., Haplosciadium abyssinicum Hochst., Mucuna melanocarpa Hochst. ex A. Rich., and Phragmanthera macrosolen (Steud. ex A. Rich.) M.G.Gilbert had the highest fidelity level values (100%) against the corresponding ailments.ConclusionThe study area is rich in a diversity of potential medicinal plants and associated indigenous knowledge. Thus, appropriate conservation actions and careful utilization are essential to counteract the rise of anthropogenic threats and to ensure the continuity of plants with the related indigenous knowledge. Additionally, the medicinal plants should be validated through experimentation to integrate local knowledge with modern medications.

Wild rice: unlocking the future of rice breeding.

Germplasm resources serve as the foundations of advancements in breeding and are crucial for maintaining food security. Wild rice species of the genus Oryza include rich sources of genetic diversity and high adaptability, making them a substantial resource for rice breeding. The discovery of wild-type cytoplasmic male sterility resources enabled the achievement of the 'three lines' goal in hybrid rice, significantly increasing rice yields. The application of resistance alleles from wild rice enables rice production to withstand losses caused by stress. Reduced genetic diversity due to rice breeding poses a significant limitation to further advances and can be alleviated through a systematic use of wild genetic resources that integrate geographic, climatic and environmental data of the original habitat, along with extensive germplasm collection and identification using advanced methods. Leveraging technological advancements in plant genomics, the understanding of genetic mechanisms and the application of artificial intelligence and gene editing can further enhance the efficiency and accuracy of this process. These advancements facilitate rapid isolation and functional studies of genes, and precise genome manipulation. This review systematically summarizes the utilization of superior genes and germplasm resources derived from wild rice sources, while also exploring the collection, conservation, identification and utilization of further wild rice germplasm resources. A focus on genome sequencing and biotechnology developments is leading to new breeding and biotechnology opportunities. These new opportunities will not only promote the development of rice varieties that exhibit high yields, superior stress resistance and high quality but also expand the genetic diversity among rice cultivars.

Gene body methylation evolves during the sustained loss of parental care in the burying beetle

Epigenetic modifications, such as 5-methylcytosine (5mC), can sometimes be transmitted between generations, provoking speculation that epigenetic changes could play a role in adaptation and evolution. Here, we use experimental evolution to investigate how 5mC levels evolve in populations of biparental insect (Nicrophorus vespilloides) derived from a wild source population and maintained independently under different regimes of parental care in the lab. We show that 5mC levels in the transcribed regions of genes (gene bodies) diverge between populations that have been exposed to different levels of care for 30 generations. These changes in 5mC do not reflect changes in the levels of gene expression. However, the accumulation of 5mC within genes between populations is associated with reduced variability in gene expression within populations. Our results suggest that evolved change in 5mC could contribute to phenotypic evolution by influencing variability in gene expression in invertebrates.

Characteristics and Influencing Factors of Landscape Pattern Gradient Transformation of Small-Scale Agroforestry Patches in Mountain Cities

Small-scale agroforestry patches possess irreplaceable value compared to large-scale patches. In southwestern mountainous cities of China, the complex terrain and urbanization have led to the presence of numerous small, fragmented agroforestry patches around urban areas. These patches serve as crucial habitats for endemic species and provide essential space for wild food sources, thereby contributing to a range of ecosystem services. Consequently, their proper conservation and utilization planning are of paramount importance. This study investigates the transformation characteristics of landscape patterns of mountainous small-scale agroforestry patches and their constituent elements across urban–rural gradients, identifying the driving factors behind these transformations to support conservation and utilization planning. From an urban–rural gradient perspective, four directional transects were selected and divided into uniform sample grids. Using Fragstats 4.3, landscape indices of small-scale agroforestry patches were calculated, analyzing the transformation characteristics of these patches and their elements across different gradients. Spearman correlation coefficients in SPSS were employed to assess the influence of terrain and relevant anthropogenic factors on the transformation of agroforestry patches. The findings reveal the following: (1) Small-scale agroforestry patches and their elements exhibit similar patterns in terms of size, fragmentation, dispersion, and connectivity, showing an “increasing trend in size and connectivity, decreasing fragmentation, and fluctuating dispersion” from urban centers to natural areas, with slight variations in orchard patches. However, patch cohesion and shape complexity display nonlinear differentiated transformation characteristics. (2) Overall, small-scale agroforestry patches are significantly influenced by anthropogenic construction factors, with the landscape pattern of forest patches notably affected by terrain factors. (3) Across urban–rural gradient zones, the landscape patterns of small-scale agroforestry patches in urban centers, suburbs, and rural natural areas are more affected by terrain factors, whereas those in urban construction zones are significantly influenced by anthropogenic construction factors. The findings of this study provide a scientific basis for the conservation and planning of mountainous small-scale agroforestry patches.

Comparative Analysis of Gracilaria chouae Polysaccharides Derived from Different Geographical Regions: Focusing on Their Chemical Composition, Rheological Properties, and Gel Characteristics.

Polysaccharides derived from diverse sources exhibit distinct rheological and gel properties, exerting a profound impact on their applicability in the food industry. In this study, we collected five Gracilaria chouae samples from distinct geographical regions, namely Rizhao (RZ), Lianyungang (LYG), Ningde (ND), Beihai (BH), and a wild source from Beihai (BHW). We conducted analyses on the chemical composition, viscosity, and rheological properties, as well as gel properties, to investigate the influence of chemical composition on variations in gel properties. The results revealed that the total sugar, sulfate content, and monosaccharide composition of G. chouae polysaccharides exhibit similarity; however, their anhydrogalactose content varies within a range of 15.31% to 18.98%. The molecular weight distribution of G. chouae polysaccharides ranged from 1.85 to 2.09 × 103 kDa. The apparent viscosity of the LYG and BHW polysaccharides was relatively high, whereas that of RZ and ND was comparatively low. The gel strength displayed a similar trend. BHW and LYG exhibited solid-like behavior, while ND, RZ, and BH demonstrated liquid-like characteristics at low frequencies. The redundancy analysis (RDA) analysis revealed a positive correlation between the texture profile analysis (TPA) characteristics and anhydrogalactose. The study could provide recommendations for the diverse applications of G. chouae polysaccharides derived from different geographical regions.

A methodological framework to characterize the wildlife-livestock interface: The case of wild boar in mainland Spain

BackgroundThe representation of wildlife-livestock interface (WLI) at an accurate spatial resolution poses several challenges. Furthermore, there is a lack of published material providing detailed descriptions of geospatial techniques for the purpose of producing visual results that are interpretable and contrastable for epidemiological analysis. ObjectivesOur aim is to develop a standardized, applicable, and scalable methodological framework for describing and characterizing the WLI across a large spatial extent. Subsequently, we aim to employ this framework to depict specific WLI based on different epidemiological scenarios determined by the abundance of wild boar (Sus scrofa) and domestic ungulates as an illustrative case, specifically focusing on mainland Spain. MethodsTo establish a methodological framework, we merged data from both wild and domestic sources into a hexagonal grid. We utilized data on wild boar hunting and the locations of pig, cattle, sheep, and goat farms in mainland Spain. New variables were derived from this combined dataset to illustrate the overlapping abundance between wild boar and domestic species. Finally, a cluster analysis of the generated variables was carried out, with the aim of distinguishing and characterizing various scenarios of the wild boar-domestic ungulate interface in mainland Spain. ResultsThe hexagonal grid proved appropriate to represent and evaluate the WLI at fine spatial resolution over such broad extent. Despite the inability to ascribe a dominant livestock type and production system to a specific region, we were able to identify fifteen main areas of interest in terms of overlap. As for extensive livestock, normally at the highest risk of interaction with wild boar, the primary regions in Spain were those with dehesa agroecosystem and the Atlantic areas. Certain scenarios were particularly relevant in terms of risk for interaction and subsequent transmission of disease, namely, the case of extensive pig production in south western Spain (dehesa agroecosystem), which is especially concerned about the potential introduction of African Swine fever (ASF) in the Country. Discussion and conclusionsWe provide a basis for visualizing and understanding of different WLI scenarios, which is extensible to other regions and interfaces, and automatable where precise source of data from wildlife and livestock are available. This spatial statistics framework enables the utilization of high-resolution data, ensuring consistency on uniform grids. This aligns with the needs of high-resolution disease dissemination models based on wildlife behaviour. Such aspects are crucial for developing risk assessment and improving strategies for the prevention, control, and eradication of shared priority emerging diseases at national and international levels, such as ASF.

Using RNA sequencing to unravel molecular changes underlying the defense response in chickpea induced by Phytophthora medicaginis.

Phytophthora root rot (PRR), caused by Phytophthora medicaginis, is a major soil-borne disease of chickpea in Australia. Breeding for PRR resistance is an effective approach to avoid significant yield loss. Genetic resistance has been identified in cultivated chickpea (Cicer arietinum) and in the wild relative C. echinospermum, with previous studies identifying independent genetic loci associated with each of these sources. However, the molecular mechanisms associated with PRR resistance are not known. RNA sequencing analysis employed in this study identified changes in gene expression in roots of three chickpea genotypes grown hydroponically, early post-infection with P. medicaginis zoospores. Analyses of differentially expressed genes (DEG) identified the activation of a higher number of non-specific R-genes in a PRR-susceptible variety than in the resistant genotypes, suggesting a whole plant resistance response occurring in chickpea against the pathogen. Contrasting molecular changes in signaling profiles, proteolysis and transcription factor pathways were observed in the cultivated and wild Cicer-derived resistant genotypes. DEG patterns supported a hypothesis that increased root elongation and reduced adventitious root formation limit the pathogen entry points in the genotype containing the wild Cicer source of PRR resistance. Candidate resistance genes, including an aquaporin and a maltose transporter in the wild Cicer source and GDSL esterases/lipases in the cultivated source of resistance, were oppositely regulated. Increased knowledge of these genes and pathways will improve our understanding of molecular mechanisms controlling PRR resistance in chickpea, and support the development of elite chickpea varieties through molecular breeding approaches.

Small translocations of endangered Gallinula galeata sandvicensis (Hawaiian Common Gallinule) may be sufficient to generate a viable reintroduced population

Abstract Where stable source populations of at-risk species exist, translocation may be a reasonable strategy for re-establishing extirpated populations. However, the success rates of such efforts are mixed, necessitating thorough preliminary investigation. Stochastic population modeling can be a useful method of assessing the potential success of translocations. Here, we report on the results of modeling translocation success for the Gallinula galeata sandvicensis or ‘alae ‘ula (Hawaiian Common Gallinule), an endangered waterbird endemic to the Hawaiian Islands. Using updated vital rates, we constructed a model simulating 3 existing extant (wild) source populations and a hypothetical recipient site on another island. We then projected the effects of 6 different translocation scenarios and sensitivity of the results to variation of three important demographic parameters on the probability of extinction (PE) of the reintroduced and donor populations. Larger translocations, of at least 30 birds, had low probability of extinction in the reintroduced population, but raised extinction risk of the smallest source population. Spacing out translocations in time (e.g., 10 birds translocated in total in 3 installments over 9 years), led to lower PE than translocating all individuals at once (i.e., bulk translocations) for both the source and reintroduced populations. Brood size and hatch-year juvenile survival had a disproportionate impact on reintroduced population viability. Importantly, the reported juvenile survival rate is very near the threshold for population failure. This suggests that post-introduction and subsequent management of wetlands, particularly predator control, could be critical to reintroduction success. We recommend that individuals should be translocated from multiple, genetically distinct subpopulations to reduce the possibility of inbreeding depression. Based on this analysis, the recipient wetland should be sufficiently large that it can support at least 25 pairs of gallinules. Based on recent estimates of population densities on O‘ahu, such a wetland would need to be between 3.75 and 74.6 ha.

Screening and Genomic Analysis of Alkaloid-Producing Endophytic Fungus Fusarium solani Strain MC503 from Macleaya cordata.

The extensive harvesting of Macleaya cordata, as a biomedicinal plant and a wild source of quaternary benzo[c]phenanthridine alkaloids, has led to a rapid decline in its population. An alternative approach to the production of these bioactive compounds, which are known for their diverse pharmacological effects, is needed. Production of these compounds using alkaloid-producing endophytic fungi is a promising potential approach. In this research, we isolated an alkaloid-producing endophytic fungus, strain MC503, from the roots of Macleaya cordata. Genomic analysis was conducted to elucidate its metabolic pathways and identify the potential genes responsible for alkaloid biosynthesis. High-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) analyses revealed the presence and quantified the content of sanguinarine (536.87 μg/L) and chelerythrine (393.31 μg/L) in the fungal fermentation extract. Based on our analysis of the morphological and micromorphological characteristics and the ITS region of the nuclear ribosomal DNA of the alkaloid-producing endophyte, it was identified as Fusarium solani strain MC503. To the best of our knowledge, there is no existing report on Fusarium solani from Macleaya cordata or other medicinal plants that produce sanguinarine and chelerythrine simultaneously. These findings provide valuable insights into the capability of Fusarium solani to carry out isoquinoline alkaloid biosynthesis and lay the foundation for further exploration of its potential applications in pharmaceuticals.

Regulatory Performance of Tourist Hunting and Legal Game Meat Trade in Tanzania

Recently, the government of Tanzania has legalized the sale of game meat under special rules namely the Wildlife Conservation (Game Meat Selling) Regulations of 2020 as an effort to curb illegal hunting. The regulations mention tourist hunting as one of the sources of commercial game meat. Other sources include resident hunting; wildlife farms/ranches/zoos; and culling, cropping, and control of problem animals. In this paper we examine the performance of tourist hunting, legal game meat in the country, and its regulatory framework from the perspectives of key stakeholders, including both public and private or non-state stakeholders. We use a variant of Likert’s methods of summated ratings and the cumulative ordinal regression model to analyse and compare the opinions of stakeholders. We found that there are still challenges, especially related to sources of wild animals. Operators of game meat facilities find it difficult to source game meat from tourist hunting companies and the latter are not willing to offer the meat to licensed operators of game meat shops. Our results support the hypothesis that authorization of the game meat trade can serve as one of the boldest conservation tools to mitigate illegal poaching if well-planned and regulated. The results of the cumulative ordinal regression model predict an improvement of 42% in the performance of regulatory framework per unit improvement in the procedures for application and registration of game meat selling facilities (p = 0.010). The paper recommends leveraging stakeholders' active engagement and strengthening cooperation between game meat value chain actors and law-enforcement authorities. An innovative participatory model for enforcing game meat sanitary and safety regulations is also recommended to avoid potential health risks to consumers. It underscores the importance of institutional capacity building, awareness creation, and adequate funding in the fight against illegal hunting and game meat trade. In order for the country to be able to achieve sustainable game meat trade in the country, the challenge of limited wild animal sources has to be addressed, possibly by supporting the establishment of privately owned wild animal farms, ranches, and zoos, this also has its own challenges that need to be addressed too.

Ex situ conservation of two rare oak species using microsatellite and SNP markers.

Plant collections held by botanic gardens and arboreta are key components of ex situ conservation. Maintaining genetic diversity in such collections allows them to be used as resources for supplementing wild populations. However, most recommended minimum sample sizes for sufficient ex situ genetic diversity are based on microsatellite markers, and it remains unknown whether these sample sizes remain valid in light of more recently developed next-generation sequencing (NGS) approaches. To address this knowledge gap, we examine how ex situ conservation status and sampling recommendations differ when derived from microsatellites and single nucleotide polymorphisms (SNPs) in garden and wild samples of two threatened oak species. For Quercus acerifolia, SNPs show lower ex situ representation of wild allelic diversity and slightly lower minimum sample size estimates than microsatellites, while results for each marker are largely similar for Q. boyntonii. The application of missing data filters tends to lead to higher ex situ representation, while the impact of different SNP calling approaches is dependent on the species being analyzed. Measures of population differentiation within species are broadly similar between markers, but larger numbers of SNP loci allow for greater resolution of population structure and clearer assignment of ex situ individuals to wild source populations. Our results offer guidance for future ex situ conservation assessments utilizing SNP data, such as the application of missing data filters and the usage of a reference genome, and illustrate that both microsatellites and SNPs remain viable options for botanic gardens and arboreta seeking to ensure the genetic diversity of their collections.

Exploring the Efficacy of Four Apiaceae Essential Oils against Nine Stored-Product Pests in Wheat Protection.

The Apiaceae family, known for aromatic plants producing bioactive essential oils (EOs), holds significance across sectors, including agrochemicals. This study evaluated the insecticidal potential of four Apiaceae EOs from Crithmum maritimum L., Trachyspermum ammi (L.) Sprague ex Turrill, Smyrnium olusatrum L., and Elwendia persica (Boiss.) Pimenov and Kljuykov against various significant storage pests (Sitophilus oryzae (L.), Trogoderma granarium Everts, Rhyzopertha dominica (F.), Tribolium castaneum (Herbst), T. confusum Jacquelin du Val, Oryzaephilus surinamensis (L.), Alphitobius diaperinus (Panzer), Acarus siro L., and Tenebrio molitor L.) on wheat. Insect mortality rates were monitored at intervals of 1, 2, 3, 4, 5, 6, and 7 days. Smyrnium olusatrum EO exhibited the highest efficacy, followed by T. ammi, C. maritimum, and E. persica EOs, although efficacy varied by species, developmental stage, and concentration. Notably, complete mortality occurred for several pests at 1000 ppm of S. olusatrum and T. ammi EOs. Gas chromatography-mass spectrometry (GC-MS) analysis revealed key compounds in these EOs, including myrcene, germacrone, and curzerene in S. olusatrum EO, and thymol, γ-terpinene, and p-cymene in T. ammi EO. These findings emphasize their potential as botanical insecticides. Smyrnium olusatrum and T. ammi EOs emerge as promising eco-friendly pest management options due to their efficacy, highlighted compound composition, and availability of biomass from both wild and cultivated sources.

Integrated Transcriptomics and Metabolomics Analysis Reveals the Effects of Cutting on the Synthesis of Flavonoids and Saponins in Chinese Herbal Medicine Astragalus mongholious.

Astragali Radix, derived from the roots of Astragalus mongholicus, is a traditional Chinese medicine containing flavonoids and saponins as its key ingredients. With a shortage in the wild sources of the herbal plant, it is especially important to explore a cultivation mode for A. mongholicus for medicinal purposes. Cutting, a physical environmental stress method, was used in this study with the objective of improving the quality of this herbal legume. We found that cutting of the top 1/3 of the aboveground part of A. mongholicus during the fruiting period resulted in a significant increase in the content of flavonoids and saponins, as well as in root growth, including length, diameter, and dry weight. Furthermore, the leaves were sampled and analyzed using a combined transcriptome and metabolome analysis approach at five different time points after the treatment. Sixteen differentially expressed unigenes (DEGs) involved in the biosynthesis of flavonoids were identified; these were found to stimulate the synthesis of flavonoids such as formononetin and calycosin-7-O-β-D-glucoside. Moreover, we identified 10 DEGs that were associated with the biosynthesis of saponins, including astragaloside IV and soyasaponin I, and found that they only regulated the mevalonic acid (MVA) pathway. These findings provide new insights into cultivating high-quality A. mongholicus, which could potentially alleviate the scarcity of this valuable medicinal plant.

Present Status, Constraints and Potentials of Mud-crab Culture in West Bengal

Background: Mud crab (Scylla serrata) farming offers better prospects for all sections of rural people, particularly those who have a poor land base and an abundant labour force. It offers reliable incomes to both small and large farming operations, but there are several barriers to crab farming that limit its potential. Methods: The present study was conducted in three randomly selected villages of Gosaba block in the South 24-Parganas district of West Bengal, which has the highest concentration of crab farmers. Three villages from the Lahiripur Gram Panchayat area of Gosaba block were selected by simple random sampling without replacement technique. A total of 60 crab farmers were randomly selected for the present study. Data were collected with the help of a specially constructed structured interview schedule and non-participant observational technique. Result: The findings of the study showed that the majority (61.67%) of the respondents practiced traditional crab culture techniques and 83.33 per cent preferred the November-February months of the year as an ideal time for crab farming. A good number of respondents (68.33%) reported that they collected seeds from wild sources, i.e., swamps and deserted water bodies. It was conspicuous from the present study that the majority of the respondents used Puntias sp. as supplementary feed. The major diseases of crabs in the study area were ulcers on carapace, necrosis of appendages and fungal diseases and they applied lime and potassium permanganate (KMnO4) to get rid of these diseases. Lack of crab seeds emerged as a prime constraint, followed by marketing problems, transport problems, credit problems and problems related to diseases.

Physicochemical and Phytochemical analysis of Wild and Cultivated Chitrak (Plumbago zeylanica Linn.)

Chitrak (Plumbago zeylanica Linn.) is a perennial shrub that grows slowly. Its roots become mature enough for medicinal use after a long time. The major source of Chitrak is wild and mainly roots are used in medicine preparation, so it needs to be uprooted and plant sustainability can’t be maintained. Therefore, this study was conducted to evaluate the physicochemical and phytochemical parameters of wild and cultivated root, stem, and leaves of Chitrak. Wild and cultivated Chitrak was collected from Kharangana forest and Herbal garden, MGACH&RC, Dist. Wardha respectively in an appropriate season as per the guidelines mentioned in Charak Samhita. Collected plant material was cleaned properly, shed-dried, ground, stored in an airtight container, and labeled for further study. For the Chromatographic study, a standard Plumbagin marker was procured from Sigma Aldrich Company, Bengaluru. This study concluded that all test samples contain Plumbagin but cultivated Chitrak root contains more Plumbagin than leaves and stem. So, according to ancient Ayurveda texts, the use of Chitrak root for various diseases as compared to leaves and stem is justified and ideal as far as the Plumbagin is concerned. But as leaves and stems also contain some amount of Plumbagin then it may be used for less chronic diseases or in patients who cannot tolerate the Tikshnatva and Ushnatva of Chitrak root. In this study, cultivated Chitrak root showed more Plumbagin than the wild source. So we can say that cultivated sources can be used in therapeutics instead of the wild sources after confirmation by clinical trials thereby minimizing the unauthorized collection practices and motivating the cultivation of this plant to some extent.

Seasonal and culture period variations in the lipid and fatty acid content of Ulva lactuca cultivated in Mikhmoret onshore (Israel)

Abstract The use of high-value fatty acids (FA), omega-3 (n-3), and omega-6 (n-6) from seaweed could relieve the pressure from natural wild fish sources and reduce overfishing worldwide. This research is designed to explore how the harvest season (winter, spring, and summer) and culture period influence the biomass production yield, lipid content, and FA composition in the green seaweed Ulva sp. The studied seaweed was grown in plastic sleeves with flowthrough seawater in Mikhmoret (East Mediterranean, Israel) from July 2019 to December 2020. The Ulva species was identified as Ulva lactuca Linnaeus by DNA barcoding using rbcL, ITS, and tufA markers. No detectable genetic variability in U. lactuca samples was found throughout the research period. A quantitative examination of the lipid and FA content in U. lactuca in different harvest seasons revealed that the maximum content of lipids was in the summer, and that of polyunsaturated fatty acids (PUFAs) was in the winter. The PUFA profile included eicosapentaenoic acid, docosapentaenoic acid, docosahexaenoic acid, n-3, and n-6, where the n-6/n-3 ratio was comparable with the desired range for a balanced nutritional diet.

PHARMACOGNOSTIC EVALUATION OF APIUM GRAVEOLENS LINN. AND TRACHYSPERMUM ROXBURGHIANUM (DC.) CRAIB.: THE DRUG “AJMODA” IN INDIAN SYSTEM OF MEDICINE

Two species, namely, Apium graveolens Linn. and Trachyspermum roxburghianum (DC.) Craib., are being popularly known as ‘Ajmoda’ in India and ‘Celery’ in English. Both the species belong to the family Apiaceae. The plant specimens are mostly collected from wild sources by unskilled collectors on the basis of the local names, which leads to adulteration and substitution for genuine drugs. There is a chance of improper identification because of similar name. The seeds of these species are morphologically very similar and it is difficult to identify because of their smaller size. Therefore, this study aims to find out the detailed pharmacognostical evaluation to differentiate the seeds of the two species on the basis of microscopic characters, fluorescence analysis, physicochemical values and TLC fingerprint profile.

COLOSSAL LOSS OF FISH BIODIVERSITY DURING FRY COLLECTION IN THE RUPSHA RIVER

A key challenge for sustainable fish production is the loss of fish biodiversity caused by the indiscriminate capture of non-target creatures, particularly in Bangladesh's inland and coastal regions. In addition to serving as a crucial navigational channel in Bangladesh's coastal regions, the Rupsha River in Khulna City is also home to over 15000 fishermen who harvest fish and shrimp fry. Therefore, a study was carried out to assess the existing fry collection scenarios, fish bycatch and its impact on the biodiversity to enhance the production and biodiversity of the Rupsha River. Between May 2022 and July 2023, information was gathered from stakeholders through a combination of personal observation, focus groups, and cross-check interviews. In this study, Macrobrachium rosenbergii and Penaeus monodon fry were collected from wild sources by fixed blue net (FBN) and drag net (DN) as target species. For FBN compared to DN, efficiency and survival rate were both higher. FBN and DN caught 20 different bycatch species, including one highly endangered (Channa striata), four vulnerable species, and four commercial species. During the study period, the mortality rates of bycatch was 50%. The assessed value of the commercial adult species lost during fry collection stands at 1,245 BDT (Bangladesh Taka) per person per day, whereas the earnings of the fry collectors’ amount to 80 BDT per day. Hence, it is imperative to rigorously enforce a ban on larvae fishing through community participation and integrated coastal zone management to preserve aquatic biodiversity and protect the livelihoods of the coastal population.

National spatial and temporal patterns of the global wildlife trade

The international wildlife trade is a vast global business contributing billions of dollars to national economies and spanning thousands of species. The Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) outlines a framework for the effective management of the global trade in threatened species. A key question is understanding how exports and imports between the 184 CITES Parties have changed across space and time, and how the successful implementation of CITES’ legal requirements impacts these patterns. Focusing on birds, mammals, amphibians, and reptiles traded from wild sources between 2000 and 2020, we highlight a small number of Parties imported or exported the majority of trade volume and that these Parties vary distinctly between taxa. Imports and exports of wild-sourced origin were mostly declining or fluctuating over time, with the majority of Parties maintaining very low but fluctuating volumes, even for Parties with high historic total volumes (>10,000 individuals). Nevertheless, reptiles had eight high-volume exporters with increasing trends, while exports of Indonesian reptiles and USA mammals had high and stable roles in trade. Parties with legislation that meets all the Convention’s legal requirements had faster-declining bird, mammal, and reptile import volumes versus those without, while export volumes were not associated with differing legislative status. This research highlights the declining role in the trade of many Parties that historically traded high volumes, but the emergence of a few newly dominant Parties as suppliers for whom confirming the robustness of their sustainability assessments is an urgent priority.