Wild Grasses Research Articles

Rice and its nutritional significance

Rice is a staple food for millions of populations in the world and originated from a wild grass to a crop. The Asian civilization was built on rice. The rice cultivation changed the lives of hunters into farmers. The domestication of rice in India dates back to the 7th millennium BC. Due to industrialization, the traditional practice of hand pounding was overtaken by rice processing mills, which led to the production of white polished rice, devoid of many nutrients. Studies confirmed that higher consumption of white rice increased the risk for diabetes and cardiovascular disease, high in India. In the current scenario, the disease prevalence is increasing, and customers are in search of nutritious foods with potential health benefits. This paper outlines the origin of rice and its nutritional value.

The Impact of Turmeric, Curcuma, and Ginger Feed Additives on the Productivity of Kaur Cattle as a Genetic Resource in Bengkulu

Kaur cattle, a local breed in Bengkulu, represent a valuable genetic resource that requires preservation and effective utilization to enhance livestock production. Turmeric, curcuma, and ginger are plants known for their essential oils and curcumin content, which offer numerous proven benefits. This study aimed to evaluate the impact of these feed additives on the productivity of Kaur cattle. A quantitative research method employing a completely randomized design was used, consisting of four treatments and four replications. The diet comprised wild grass (10% of body weight) and palm kernel meal in an 80:20 ratio. The treatments were as follows: P0 (control: wild grass and palm kernel meal), P1 (wild grass, palm kernel meal, and turmeric powder Curcuma xanthorrhiza Roxb.), P2 (wild grass, palm kernel meal, and curcuma powder Curcuma longa L.), and P3 (wild grass, palm kernel meal, and ginger powder Zingiber officinale Rosc.). The study involved 16 male Kaur cattle aged 1.5-2 years, with an average body weight of 160-180 kg, observed over 60 days. The variables measured included average daily weight gain, forage consumption, total feed consumption, feed efficiency, and cost efficiency. Data were analyzed using a one-way ANOVA. The results showed that the mean daily weight gain (kg/head/day) was P0=0.35±2.57, P1=0.47±2.39, P2=0.50±2.46, and P3=0.49±2.88, with no significant differences between treatments. The mean feed efficiency (%) was P0=0.12±4.44, P1=0.25±4.71, P2=0.27±5.22, and P3=0.26±4.38, also showing no significant differences. The mean cost efficiency for feed use was P0=35,869±7.47, P1=34,281±7.08, P2=33,220±7.39, and P3=35,410±7.21, with no significant differences observed. Overall, the addition of turmeric, curcuma, and ginger powders to the feed did not significantly impact the productivity of Kaur cattle.

226 Serotonin stimulates relaxation in ovine saphenous vein precontracted with ergovaline

Abstract The predominant ergot alkaloid found in wild type endophyte-infected tall fescue grass is ergovaline. Ergovaline is a potent vasoconstrictor in livestock that consume it. Previous research has shown that ergovaline can bind serotonin (5-HT) receptors and blood vessels exposed to ergovaline become unresponsive to subsequent 5-HT exposure. Serotonin can stimulate vascular contraction or relaxation depending on the receptor subtype targeted. The objective of this experiment was to evaluate 5HT as vasorelaxant in the ovine saphenous artery and vein that were precontracted with ergovaline. Blood vessels were collected from mixed breed market rams (n = 7) at slaughter, cleaned of surrounding adipose and connective tissue, sliced into 2-mm cross-sections, and mounted in a multimyograph. Artery and vein cross-sections were equilibrated to 1 g tension in continuously gassed (95% O2/5% CO2) Krebs-Henseleit buffer (pH 7.4; 37.5 °C) for 90 min, precontracted with 1.0 μM ergovaline (in a tall fescue seed extract) for 30 min, exposed to increasing concentrations of 5-HT that ranged from 1 x 10-9 to 1 x 10-4 M for a 5-min interval. Response data were normalized to the 1.0 μM ergovaline response and were analyzed as a completely randomized design in SAS. The saphenous vein was larger in diameter blood vessel than the saphenous artery. Both vessel types contracted in response to exposure to 1 μM ergovaline. The saphenous artery had a much lower maximal response to ergovaline than did the vein (P < 0.05) that could be attributed to the size difference. The lateral saphenous vein relaxed to increasing concentrations of 5-HT (P < 0.05) with 87 % relaxation occurring by 1 x 10-6 M 5-HT. Conversely, the lateral saphenous artery contracted in response to increasing concentrations of 5-HT (P < 0.05) resulting in a 43% increase from the ergovaline response by the 1 x 10-4 M addition. This response did not become significant in the artery until 1 x 10-5 M 5-HT compared with 1 x 10-8 M for the vein. This resulted in a 50% effective concentration for 5-HT in the artery of 4.5 compared with 8.1 M for the vein (P < 0.05). The differing responses across vessels are likely a result of different receptor populations and this should be verified in future research. This is the first reported observation of stimulated relaxation of a blood vessel constricted ergovaline. Additional research is needed to understand the mechanism of how 5HT can be used to stimulate vasorelaxation in livestock suffering from ergot alkaloid-induced vasoconstriction.

New Insights into Phytolith Characteristics in Wild Grass as the Source of Sustainable Silicon Oxide Anodes

New Insights into Phytolith Characteristics in Wild Grass as the Source of Sustainable Silicon Oxide Anodes

Genome architecture of the allotetraploid wild grass Aegilops ventricosa reveals its evolutionary history and contributions to wheat improvement

The allotetraploid wild grass Aegilops ventricosa (2n = 4x = 28, genome DvDvNvNv) has been recognized as an important germplasm resource for wheat improvement owing to its ability to tolerate biotic stresses. In particular, the 2NvS segment from Ae. ventricosa, as a stable and effective resistance source, has contributed greatly to wheat improvement. The 2NvS/2AS translocation is a prevalent chromosomal translocation between common wheat and wild relatives, ranking just behind the 1B/1R translocation in importance for modern wheat breeding. Here, we assembled a high-quality chromosome-level reference genome of Ae. ventricosa RM271 with a total length of 8.67 Gb. Phylogenomic analyses revealed that the progenitor of the Dv subgenome of Ae. ventricosa is Ae. tauschii ssp. tauschii (genome DD); by contrast, the progenitor of the D subgenome of bread wheat (Triticum aestivum L.) is Ae. tauschii ssp. strangulata (genome DD). The oldest polyploidization time of Ae. ventricosa occurred ∼0.7 mya. The Dv subgenome of Ae. ventricosa is less conserved than the D subgenome of bread wheat. Construction of a graph-based pangenome of 2AS/6NvL (originally known as 2NvS) segments from Ae. ventricosa and other genomes in the Triticeae enabled us to identify candidate resistance genes sourced from Ae. ventricosa. We identified 12 nonredundant introgressed segments from the Dv and Nv subgenomes using a large winter wheat collection representing the full diversity of the European wheat genetic pool, and 29.40% of European wheat varieties inherit at least one of these segments. The high-quality RM271 reference genome will provide a basis for cloning key genes, including the Yr17-Lr37-Sr38-Cre5 resistance gene cluster in Ae. ventricosa, and facilitate the full use of elite wild genetic resources to accelerate wheat improvement.

Persistent legacy effects on soil metagenomes facilitate plant adaptive responses to drought.

Both chronic and acute drought alter the composition and physiology of soil microbiomes, with implications for globally important processes including carbon cycling and plant productivity. When water is scarce, selection favors microbes with thicker peptidoglycan cell walls, sporulation ability, and constitutive osmolyte production (Schimel, Balser, and Wallenstein 2007)-but also the ability to degrade complex plant-derived polysaccharides, suggesting that the success of plants and microbes during drought are inextricably linked. However, communities vary enormously in their drought responses and subsequent interactions with plants. Hypothesized causes of this variation in drought resilience include soil texture, soil chemistry, and historical precipitation patterns that shaped the starting communities and their constituent species (Evans, Allison, and Hawkes 2022). Currently, the physiological and molecular mechanisms of microbial drought responses and microbe-dependent plant drought responses in diverse natural soils are largely unknown (de Vries et al. 2023). Here, we identify numerous microbial taxa, genes, and functions that characterize soil microbiomes with legacies of chronic water limitation. Soil microbiota from historically dry climates buffered plants from the negative effects of subsequent acute drought, but only for a wild grass species native to the same region, and not for domesticated maize. In particular, microbiota with a legacy of chronic water limitation altered the expression of a small subset of host genes in crown roots, which mediated the effect of acute drought on transpiration and intrinsic water use efficiency. Our results reveal how long-term exposure to water stress alters soil microbial communities at the metagenomic level, and demonstrate the resulting "legacy effects" on neighboring plants in unprecedented molecular and physiological detail.

Multiple Horizontal Mini-chromosome Transfers Drive Genome Evolution of Clonal Blast Fungus Lineages.

Crop disease pandemics are often driven by asexually reproducing clonal lineages of plant pathogens that reproduce asexually. How these clonal pathogens continuously adapt to their hosts despite harboring limited genetic variation, and in absence of sexual recombination remains elusive. Here, we reveal multiple instances of horizontal chromosome transfer within pandemic clonal lineages of the blast fungus Magnaporthe (Syn. Pyricularia) oryzae. We identified a horizontally transferred 1.2Mb accessory mini-chromosome which is remarkably conserved between M. oryzae isolates from both the rice blast fungus lineage and the lineage infecting Indian goosegrass (Eleusine indica), a wild grass that often grows in the proximity of cultivated cereal crops. Furthermore, we show that this mini-chromosome was horizontally acquired by clonal rice blast isolates through at least nine distinct transfer events over the past three centuries. These findings establish horizontal mini-chromosome transfer as a mechanism facilitating genetic exchange among different host-associated blast fungus lineages. We propose that blast fungus populations infecting wild grasses act as genetic reservoirs that drive genome evolution of pandemic clonal lineages that afflict cereal crops.

Stomatal dynamics in Alloteropsis semialata arise from the evolving interplay between photosynthetic physiology, stomatal size and biochemistry.

C4 plants are expected to have faster stomatal movements than C3 species because they tend to have smaller guard cells. However, little is known about how the evolution of C4 photosynthesis influences stomatal dynamics in relation to guard cell size and environmental factors. We studied photosynthetically diverse populations of the grass Alloteropsis semialata, showing that the origin of C4 photosynthesis in this species was associated with a shortening of stomatal guard and subsidiary cells. However, for a given cell size, C4 and C3-C4 intermediate individuals had similar or slower light-induced stomatal opening speeds than C3 individuals. Conversely, when exposed to decreasing light, stomata in C4 plants closed as fast as those in non-C4 plants. Polyploid formation in some C4 plants led to larger stomatal cells and was associated with slower stomatal opening. Conversely, diversification of C4 diploid plants into wetter environments was associated with an acceleration of stomatal opening. Overall, there was significant relationship between light-saturated photosynthesis and stomatal opening speed in the C4 plants, implying that photosynthetic energy production was limiting for stomatal opening. Stomatal dynamics in this wild grass therefore arise from the evolving interplay between photosynthetic physiology and the size and biochemical function of stomatal complexes.

Temporal resource continuity for egg parasitoids of Dalbulus maidis (Hemiptera: Cicadellidae) during winter on irrigated maize crops and edge grasses.

Little is known about winter-season parasitism of eggs of the corn leafhopper Dalbulus maidis DeLong (Hemiptera: Cicadellidae), an important pest of maize throughout the Americas. Our study, conducted in Mexico, aimed to characterize winter-season parasitism of corn leafhopper eggs on maize crops cultivated with drip irrigation and on wild grasses that grow on the edges of maize crops when maize is not present. Maize leaves baited with D. maidis eggs were used to trap the egg parasitoids in the field. In the first year (2022), parasitism of D. maidis eggs was investigated in maize fields planted contiguously on different dates (asynchronous planting). In the second year (2023), parasitism of D. maidis eggs was evaluated in edge grasses and in adjacent maize crops planted on the same date (synchronous). The highest percentage of parasitism (53%), percentage of emergence, and total abundance of egg parasitoids were found in asynchronous maize fields. Here, Anagrus virlai Triapitsyn (Hymenoptera: Mymaridae), Paracentrobia subflava (Girault) (Hymenoptera: Trichogrammatidae), and Pseudoligosita sp. (Hymenoptera: Trichogrammatidae) wasps were found parasitizing the D. maidis eggs, with P. subflava being the most abundant. In wild edge grasses, only P. subflava was found, showing low levels of parasitism, while in synchronous maize, P. subflava increased its percentage of parasitism (up to 37%), percentage of emergence, and abundance, during winter. These results suggest that P. subflava acts as an efficient biological control agent of D. maidis in irrigation-grown maize crops during the winter season, and that edge grasses are overwinter habitats for P. subflava.

Assessment of the resistance of wild grasses in the northwestern region of the Russian Arctic to chloride salinization

Wild grasses are prevalent across various types of vegetation, playing a significant role in both natural ecosystems and human economic endeavors. The potential of using grasses to restore areas affected by tidal erosion and saline soils has not been fully investigated. A study was conducted to examine the impact of different levels of NaCl salinity (ranging from 20 to 200 mM) on seed germination and early growth of Agrostis capillaris L., Deschampsia cespitosa (L.) P. Beauv., Phalaris arundinacea L., and Phleum pratense L. These plants are commonly found in the Arctic region within the Belomorsky district of the Republic of Karelia. Through research, a direct connection was discovered between the germination of seeds and the growth of seedlings based on the salt levels in the root environment and the type of grass species present. In summary, all species successfully germinated at NaCl concentrations of 20–80 mM. However, germination rates decreased at 100 and 200 mM in most cases. When exposed to NaCl concentrations of 60 mM and higher, the growth of root and shoot in the grasses was slowed down to some extent (depending on the species), but not completely inhibited. Due to the resilience of wild grasses to cold temperatures, it is proposed that they could be used in the phytoremediation of Arctic areas with a salinity level of up to 100 mM (0.6 % salinity). An analysis of the data resulted in a ranking of grass species based on their salt resistance: P. pratense ˃A. capillaris ˃P. arundinacea ˃D. cespitosa.

Wild grass-derived alleles represent a genetic architecture for the resilience of modern common wheat to stresses.

This review explores the integration of wild grass-derived alleles into modern bread wheat breeding to tackle the challenges of climate change and increasing food demand. With a focus on synthetic hexaploid wheat, this review highlights the potential of genetic variability in wheat wild relatives, particularly Aegilops tauschii, for improving resilience to multifactorial stresses like drought, heat, and salinity. The evolutionary journey of wheat (Triticum spp.) from diploid to hexaploid species is examined, revealing significant genetic contributions from wild grasses. We also emphasize the importance of understanding incomplete lineage sorting in the genomic evolution of wheat. Grasping this information is crucial as it can guide breeders in selecting the appropriate alleles from the gene pool of wild relatives to incorporate into modern wheat varieties. This approach improves the precision of phylogenetic relationships and increases the overall effectiveness of breeding strategies. This review also addresses the challenges in utilizing the wheat wild genetic resources, such as the linkage drag and cross-compatibility issues. Finally, we culminate the review with future perspectives, advocating for a combined approach of high-throughput phenotyping tools and advanced genomic techniques to comprehensively understand the genetic and regulatory architectures of wheat under stress conditions, paving the way for more precise and efficient breeding strategies.

PENGARUH KEKUATAN IMPAK DAN TARIK KOMPOSIT BERPENGUAT SERAT SEMBUKAN DENGAN MATRIK EPOXY

Composite is a combination of two or more elements arranged in a certain way to get new properties in matrial. Plants of Pukan (Peaderiafoetida L.) is a kind of wild grass that spreads and grows wild in nature. This study aims to determine the effect of the mechanical strength of the composite reinforced dying in the Healing with epoxy matrix. In this test, using Sembukan fiber as a reinforcement and epoxy as a composite matrial adhesive. The tests include: Tensile tests referring to ASTMD 3039, and Impact Tests referring to ASTMD 256. These tests use fiber volume fractions of 20%, 30%, and 40% with woven fiber orientation. The results showed, the greater the fiber volume fraction, the greater the toughness, the highest toughness was found in the fiber volume fraction variance of 40%, amounting to 0.10396 J/mm2. Same as Tensile testing, the stress increases with increasing fiber volume fraction, ie at 40% fiber at 9,1596 MPa. The influencing factor is none other than the percentage of fibers in the composite increasing the mechanical strength of the matrix. Keywords: Fiber Heal, toughness Impact, Tensile strength.

Investigation of biological-rhythm patterns: physiological and biochemical effects in herbaceous plants exposed to low-level chronic radiation – part 1: nonirradiated seeds

Hypothesis Because reactive oxygen species are involved in the regulation of biological rhythms, we hypothesized that intra-annual variability of seed progeny quality at low doses of ionizing radiation (LDIRs) would differ from that of background plants. Materials and methods We conducted 12 consecutive experiments using the roll culture method by germinating seeds (monthly for 3 weeks) of six herbaceous plant species (Bromus inermis, Geum aleppicum, Plantago major, Rumex confertus, Silene latifolia, and Taraxacum officinale) growing under conditions of chronic radiation in the East Ural Radioactive Trace (EURT). We assessed physiological (seed viability and abnormality frequency) and biochemical (low-molecular-weight antioxidants, LMWAs) parameters of seedlings. Results Total absorbed dose rates of maternal plants (TADRplants) and seed embryos (TADRseeds) in the EURT exceeded background levels by 1–3 and 1–2 orders of magnitude, respectively. Nonlinear dependencies on TADR were mainly characteristic of physiological and biochemical parameters. For most populations of the studied species (B. inermis, G. aleppicum, R. confertus, and S. latifolia), seedling survival and root length decreased in the autumn–winter period, while the frequency of abnormal seedlings increased. The content of LMWAs could be ranked as R. confertus > B. inermis > G. aleppicum > S. latifolia, in good agreement with the presence of anthocyanin pigmentation in the plants. The lowest synthesis of antioxidants in seedlings was observed in winter. A high LMWA content promoted growth and reduced the frequency of abnormal seedlings. Conclusions These results underscore a multistage nature of the impact of LDIRs on intra-annual biological rhythms in plants. High heterogeneity in reference group ‘wild grasses’ and diversity of their radiobiological effects should help to develop methods of radiation protection for natural ecosystems and facilitate approaches used by the International Commission on Radiological Protection. Highlights Absorbed dose rates for six plant species in the East Ural Radioactive Trace (EURT) area range from 0.11 to 73.89 µGy h−s (plants) and 0.11 to 6.88 µGy h−s (seed embryos). Intra-annual rhythms of physiological and biochemical parameters in the EURT zone differ from those in background seedlings. Plants in the EURT area exhibit a wide range of trait variability, asynchrony of the manifestation of the effects, nonlinear dose–response relations, and hormesis. A high content of low-molecular-weight antioxidants (LMWAs) is associated with low frequency of developmental abnormalities and high viability of seed progeny.

Old Foe, New Host: Epidemiology, Genetic Diversity, and Pathogenic Characterization of Maize Streak Virus in Rice Fields from Burkina Faso

Rice is of critical significance regarding food security worldwide, including in Africa. Only two viruses impacting rice production in Africa have been deeply investigated for decades: the rice yellow mottle virus ( Solemoviridae) and the rice stripe necrosis virus ( Benyviridae). Using viral metagenomics, we aimed at broadening knowledge on interacting communities associated with plants in rice landscapes and exploring the diversity and epidemiological status of viruses circulating in rice fields from Burkina Faso. We performed an epidemiological survey in this country between 2016 and 2019 involving 57 small farmers’ rice fields under two production systems: rainfed lowlands and irrigated areas. More than 2,700 rice samples were collected without regard to disease symptoms following a regular scheme. Wild and cultivated Poaceae (maize and sugarcane) growing in nearby rice fields were also collected. Unexpectedly, metagenomics detected maize streak virus (MSV) ( Geminiviridae) in analyzed rice samples. Further molecular analyses using rolling circle amplification-polymerase chain reaction showed that MSV was widely distributed and highly prevalent in both rainfed lowlands and irrigated rice areas. MSV-A and MSV-G strains were identified. MSV-G, exclusively identified thus far in wild grasses, was the most prevalent strain, whereas MSV-A, known to cause severe symptoms in maize, was sporadically identified. Using infectious clones in experimental conditions, we confirmed the pathogenicity of both MSV strains in rice. Thus, in addition to contributing to the epidemiological surveillance of rice production in Africa, our results illuminate new epidemiological and pathogenic aspects of one of the most studied plant viruses with significant economic consequences in Africa. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

Japanese Planocerid Flatworms: Difference in Composition of Tetrodotoxin and Its Analogs and the Effects of Ingestion by Toxin-Bearing Fishes in the Ryukyu Islands, Japan

Tetrodotoxin (TTX), known as pufferfish toxin, is a potent neurotoxin blocking sodium channels in muscle and nerve tissues. TTX has been detected in various taxa other than pufferfish, including marine polyclad flatworms, suggesting that pufferfish toxin accumulates in fish bodies via food webs. The composition of TTX and its analogs in the flatworm Planocera multitentaculata was identical to those in wild grass puffer Takifugu alboplumbeus. Previously, Planocera sp. from Okinawa Island, Japan, were reported to possess high level of TTX, but no information was available on TTX analogs in this species. Here we identified TTX and analogs in the planocerid flatworm using high-resolution liquid chromatography–mass spectrometry, and compared the composition of TTX and analogs with those of another toxic and non-toxic planocerid species. We show that the composition of TTX and several analogs, such as 5,6,11-trideoxyTTX, dideoxyTTXs, deoxyTTXs, and 11-norTTX-6(S)-ol, of Planocera sp. was identical to those of toxic species, but not to its non-toxic counterpart. The difference in the toxin composition was reflected in the phylogenetic relationship based on the mitochondrial genome sequence. A toxification experiment using predatory fish and egg plates of P. multitentaculata demonstrated that the composition of TTX and analogs in wild T. alboplumbeus juveniles was reproduced in artificially toxified pufferfish. Additionally, feeding on the flatworm egg plates enhanced the signal intensities of all TTX compounds in Chelonodon patoca and that of deoxyTTXs in Yongeichthys criniger.

The evolution of transposable elements in Brachypodium distachyon is governed by purifying selection, while neutral and adaptive processes play a minor role.

Understanding how plants adapt to changing environments and the potential contribution of transposable elements (TEs) to this process is a key question in evolutionary genomics. While TEs have recently been put forward as active players in the context of adaptation, few studies have thoroughly investigated their precise role in plant evolution. Here, we used the wild Mediterranean grass Brachypodium distachyon as a model species to identify and quantify the forces acting on TEs during the adaptation of this species to various conditions, across its entire geographic range. Using sequencing data from more than 320 natural B. distachyon accessions and a suite of population genomics approaches, we reveal that putatively adaptive TE polymorphisms are rare in wild B. distachyon populations. After accounting for changes in past TE activity, we show that only a small proportion of TE polymorphisms evolved neutrally (<10%), while the vast majority of them are under moderate purifying selection regardless of their distance to genes. TE polymorphisms should not be ignored when conducting evolutionary studies, as they can be linked to adaptation. However, our study clearly shows that while they have a large potential to cause phenotypic variation in B. distachyon, they are not favored during evolution and adaptation over other types of mutations (such as point mutations) in this species.

The evolution of transposable elements in Brachypodium distachyon is governed by purifying selection, while neutral and adaptive processes play a minor role

Understanding how plants adapt to changing environments and the potential contribution of transposable elements (TEs) to this process is a key question in evolutionary genomics. While TEs have recently been put forward as active players in the context of adaptation, few studies have thoroughly investigated their precise role in plant evolution. Here, we used the wild Mediterranean grass Brachypodium distachyon as a model species to identify and quantify the forces acting on TEs during the adaptation of this species to various conditions, across its entire geographic range. Using sequencing data from more than 320 natural B. distachyon accessions and a suite of population genomics approaches, we reveal that putatively adaptive TE polymorphisms are rare in wild B. distachyon populations. After accounting for changes in past TE activity, we show that only a small proportion of TE polymorphisms evolved neutrally (&lt;10%), while the vast majority of them are under moderate purifying selection regardless of their distance to genes. TE polymorphisms should not be ignored when conducting evolutionary studies, as they can be linked to adaptation. However, our study clearly shows that while they have a large potential to cause phenotypic variation in B. distachyon, they are not favored during evolution and adaptation over other types of mutations (such as point mutations) in this species.

The development of crop production in the northern Horn of Africa: a review of the archaeobotanical evidence

ABSTRACT This article presents a synthesis of the hypotheses and evidence for plant domestication and the origins of agriculture in the northern Horn of Africa. To date, available archaeological data point to an introduction of agricultural practices to Ethiopia during the late Holocene, c. 1600 BC, influenced by adjacent areas including eastern Sudan, Egypt and southern Arabia. The archaeobotanical record shows that farming in the northern highlands was based on the cultivation of barley, linseed and lentils, combined with the exploitation of local wild grasses from the Panicoideae and Chloridoideae sub-families. This indicates that local populations also played a role in the development of productive activities in the region, a process that might have been in place before the arrival of the southwest Asian agricultural package. After the first millennium BC, amidst an increase in sedentary settlements in the region, new domesticated crops appeared in the archaeological record. These include exogenous crops such as emmer wheat, but also indigenous plants such as t’ef and noog, which were locally domesticated likely throughout the Pre-Aksumite period. With the rise of the Aksumite Kingdom, c. 50 BC – AD 700, the agricultural package again expanded to include of a wide range of pulses, geophytes and other economic crops. Macrobotanical remains of sorghum and finger millet also appear for the first time during this period, although this contrasts with the microbotanical record of the region, which points to an earlier presence. In the southern highlands and southwest Ethiopia, evidence of Plectranthus edulis (Vatke) Agnew (Ethiopian potato or Oromo potato) has been identified by the late first millennium BC, pointing towards an earlier domestication than previously considered. Similarly, enset and coffee remains have been documented during the early first millennium AD. Altogether, new studies are needed to confirm some of these hypotheses, as archaeobotanical studies in the northern Horn are still limited. The combination of macrobotanical and microbotanical data, along with ethnoarchaeological and experimental research programs and linguistic studies, will be of critical importance for refining our current understanding of the processes associated with plant domestication and the introduction of crop production in the region.

Walking With A Camera

In his work, the author primarily focuses on the urban landscape and its impacts on human life. His entire documentary work is linked by a unified authorial idea. In the “Jungle City” cycle, he works with the visual counterpart of the current form of the Bratislava metropolis, full of transportation routes, dense modern development, and concrete surfaces. The means to create unexpected views of the city for him are carefully selected locations on the urban peripheries surrounded by dense vegetation – trees and wild grasses. He thus achieves a completely new view of Bratislava, in which it returns to the wilderness. The project “Quiet Sunday Afternoons in the Town by the Refinery” is the author’s reflection on the impacts of industry in the rural surroundings. The focal point is the technical structure of an oil factory, which covers a significant part of an otherwise tranquil landscape of a small Austrian town. However, through austere visual expression, Korček delivers an urgent message about the adverse impact of the factory on the ecosystem. As part of the series “Here Is Our Paradise”, which unifies the theme of lawns and trees, Korček addresses the disruption of the stereotype of urban housing estates through the small self-help interventions of their residents. While walking with a camera, he supplements these plants with various found mixtures of folk interventions placed in front of panel buildings. In the “Searching” series, he adopts the position of an observer of urban movement, consisting of figures of pedestrians in narrow rays of the low-lying sun. This time he works in the environment of New York, but unlike the previous series, he does not specify this location, only utilizing its elemental potential. Korček does not work with photographic effects; he fully utilizes the poetics of the chosen locations. He skillfully works with perspective, multiple plans, or the selection of a location. Through their specific combination, he succeeds in creating authentic images of the location, in which we still find a lot of personal fantasy.

Evaluating the use of common grasses by the wheat stem sawfly (Hymenoptera: Cephidae) and its native parasitoids in rangeland and Conservation Reserve Program grasslands.

The wheat stem sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae), is a major pest of wheat (Triticum aestivum L., Poales: Poaceae) across the northern Great Plains of North America. Cephus cinctus has a wide host range, attacking numerous wild grasses and cultivated cereals in crop and grassland habitats, where it is, in turn, attacked by 2 native braconid parasitoids. Quantitative assessments of C. cinctus infestation and parasitism levels in different grass species across the full spectrum of available hosts are important in assessing the extent to which grasslands, or specific constituent grass species, may be reservoirs of pests or parasitoids moving into wheat. We quantified infestation and parasitism levels in over 25,000 stems collected from 17 grass species and wheat spanning 35 sites in central Montana, United States, over 2 yr. Infestation levels in 5 grass species, primarily wheatgrasses, were high (38%-65%) and similar to the levels observed in wheat (55%). In contrast, the majority of grass species (12 of 17) had significantly lower levels of infestation (<10%), suggesting that most grasses are not important reservoirs of C. cinctus. Parasitism levels in highly infested wheatgrasses were, on average, 3 times higher than those in cultivated wheat, suggesting that these grasses could provide important conservation habitat for parasitoids. Future work examining the relative performance of pests and parasitoids in these grasses will be important in gauging their relative value as plant materials to bolster parasitoid conservation in reseeded grassland habitats.