Whole-crop Silage Research Articles

Post-heading accumulation of nonstructural carbohydrates and nitrogen in rice (Oryza sativa L.) roots

ContextRoots are plant organs that absorb nutrients and water. While roots serve as sink organs for nutrients in perennial and woody plant species, the dynamics of nutrient accumulation in roots for annual plant species, particularly in crop species like Oryza sativa L. (rice), are not well understood. Japanese rice cultivars bred for whole-crop silage exhibit large aboveground biomass and a notably small number of spikelets. This sink-source imbalance leads to the accumulation of non-structural carbohydrates (NSC) in stems after heading. Research questionIt is hypothesized that rice cultivars with substantial aboveground biomass might also develop significant belowground biomass. Consequently, the sink-source imbalance may result in NSC accumulation in roots. This study aims to test two hypotheses: firstly, that newly developed small-panicle rice cultivars accumulate NSC in roots post-heading, and secondly, that this post-heading NSC accumulation in roots can be influenced by cultural practices affecting spikelet number per panicle. MethodsTwo small-panicle cultivars, Tachisuzuka and Tachiayaka, and two large-panicle cultivars, Kusanohoshi and Hoshiaoba, were grown using different methods of three cultural practices: nitrogen application, transplanting season, and planting density. Measurements of dry matter, NSC (starch and sugars), and nitrogen in each plant part (panicle, leaf, stem, and roots) were taken over time after heading. The spikelet number per panicle was also counted. ResultsPost-heading, the small-panicle cultivars exhibited NSC accumulation in roots, a phenomenon not observed in the large-panicle cultivars. Although the three cultural practices altered the spikelet number per panicle and thus the sink capacity of the small-panicle cultivars, root NSC accumulation remained unaffected. Additionally, nitrogen weight per unit field area increased with increases in root weight in the small-panicle cultivars. ConclusionsThe imbalance in the sink-source relationship is likely responsible for the post-heading NSC accumulation in roots observed in small-panicle cultivars. However, the lack of root response in NSC accumulation to changes in panicle sink capacity suggests other mechanisms might be involved in root NSC accumulation. Notably, significant amounts of NSC and nitrogen remain in the roots of small-panicle cultivars after harvest. ImplicationsThe accumulation of NSC and nitrogen in roots post-heading could potentially enhance crop growth in subsequent years.

Adapting the grassland model BASGRA to simulate yield and nutritive value of whole-crop barley

Ensiling of whole-crop biomass of barley before full maturity is common practice in regions with a short growing season. The developmental stage of barley at harvest can have a large impact on yield and nutritive composition. The relationships between crop growth, environmental conditions and crop management can be described in process-based simulation models. Some models, including the Basic Grassland (BASGRA) model, have been developed to simulate the yield and nutritive value of forage grasses, and usually evaluated against metrics of relevance for whole-crop silage. The objectives of this study were to: i) modify the BASGRA model to simulate whole-crop spring barley; ii) evaluate the performance of this model against empirical data on dry matter (DM) yield and nutritive value attributes from field experiments, divided into geographical regions; and iii) evaluate DM yield, nutritive value and cutting date under current and future climate conditions for three locations in Sweden and four cutting regimes. Main model modifications included addition of a spike pool, equations for carbon (C) and nitrogen (N) allocation to the spike pool and equations for C and N translocation from vegetative plant parts to spikes. Model calibration and validation against field trial data from Sweden, including samples harvested from late anthesis stage to hard dough stage that were either pooled or divided into regions, showed better prediction accuracy, evaluated as normalised root mean squared error (RMSE), of neutral detergent fibre (NDF) (7.58–18.4%) than of DM yield (16.8–27.8%), crude protein (15.5–23.2%) or digestible organic matter in the DM (DOMD) (12.0–22.2%). Model prediction using weather data representing 1990–2020 and 2021–2040 climate conditions for three locations in Sweden (Skara, Umeå, Uppsala) showed lower DM yield, earlier harvest and slightly higher NDF concentration on average (across locations and developmental stage at cutting) when using near-future climate data rather than historical data. The model can be used to evaluate whole-crop barley performance under production conditions in Sweden or in other countries with similar climate, soils and crop management regimes.

Close relationship between potassium accumulation in internodes and translocation of non-structural carbohydrate to grains in rice cultivars with different yields

Potassium (K) is an important macronutrient together with nitrogen and phosphorous. Potassium mostly accumulates in the internodes of rice plants during ripening stage. Since internodes are responsible for translocation of carbohydrates to the grains during ripening, the involvement of internode K in grain yield has been suggested, however, the precise mechanism remains unclear. This study aims to examine the relationship between rice grain yield in different cultivars and the dynamics of K and non-structural carbohydrates (NSC) in the internodes during the ripening period. Field experiments were conducted to investigate the differences in the dynamics of K and NSCs in internodes from heading to maturation in 10 cultivars with different yields and three mutants with different grain numbers. To reveal the localization of K in internode tissues, we conducted laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) analysis. During the early ripening period from heading to 14 days after heading (14 DAH), the decrease in NSC contents in lower internodes was higher in the high-yielding indica cultivars than in japonica cultivars. In the late ripening period from 14 DAH to maturing, re-accumulation of NSC in lower internodes was higher in the japonica than in the indica. In contrast, the increase in K contents in the internodes was larger in the indica than in the japonica throughout the ripening stage. In the internodes of low-yielding whole crop silage (WCS) cultivar, the NSC contents were maintained at high levels during ripening, while K contents were consistently low. Similar results were observed in the mutant with low grain numbers. The mutant had lower K contents and higher NSC contents than the wild type (WT). Correlation analysis of different cultivars indicated that the change in K content in whole internodes from heading to maturing was negatively correlated with the change in NSC content. LA-ICP-MS analysis of internode tissues of the indica cultivar revealed that K accumulated more in the parenchyma cells than in large vascular bundles. These results suggest that K accumulation in the internodes is positively correlated with NSC translocation from internodes to grains, which could be through indirect effect of K accumulation in parenchyma on enhancement of long distance transport of NSC from internodes to the grains. This study provides new insights into the mechanism underlying the role of K in grain filling, which would help improving usage of K fertilizer for optimum plant growth and yields.

Performance of beef animals after different corn harvest strategies in a crop-livestock integrated system

The objective of this experiment was to evaluate three harvest strategies of corn intercropped with pigeon pea + Palisade grass on weight gain since grazing through finishing. A triple intercropping of corn + pigeon pea + Palisade grass was established in nine hectares (ha) during the 2016/17 season. Treatments were determined according to each corn harvest strategy: whole crop silage (WCS), high moisture corn (HMC) or corn grain (CG). The latter was not harvested due to a low yield and difficulties to hire a combine harvester for only three ha. After harvest, the 9-ha area was divided in six 1.5-ha pens for the grazing phase of the experiment with 30 non-castrated Nelore steers. The same animals were fed feedlot diets based on three sources of corn following the same harvest strategy (treatments): WCS, HMC or CG (bought from a feed industry). During the grazing phase the area that was supposed to be harvested for CG resulted in the greatest (p≤0.05) stocking rate (3.02 LU/ha) and weight gain per area (210 kg of BW/ha) compared with WCS and HMC. There was no response (p≥0.05) of corn source (according to each harvest strategy) on feed conversion ratio (FCR), body weith (BW) growth, subcutaneous fat thickness (SFT), pre-slaughter BW, hot carcass weight (HCW) and dressing percentage (DP) during the finishing phase. The data reported here may be a valuable guideline for decision making according to the beef production system and economic scenario.

Effects of forage and grain legume-based silages supplemented with faba bean meal or rapeseed expeller on lactational performance, nitrogen utilization, and plasma amino acids in dairy cows

The objective of this experiment was to investigate the effect of forage type [red clover (51%)-grass silage, i.e., RCG; vs. faba bean (66%)-grass silage, i.e., FBG] and concentrate type (faba bean, FB; vs. rapeseed expeller, RE) on lactational performance, milk composition and nitrogen (N) utilization in lactating dairy cows. Eight lactating multiparous Nordic Red cows were used in a replicated 4 × 4 Latin Square experiment, with 21-d periods, in a 2 × 2 factorial arrangement of treatments. The experimental treatments were as follows: (1) RCG with RE, (2) RCG with FB, (3) FBG with RE, and (4) FBG with FB. Inclusion rates of RE and FB were isonitrogenous. Crude protein contents of the experimental diets were 16.3, 15.9, 18.1, and 17.9% of dry matter, respectively. All diets included oats and barley and were fed ad libitum as total mixed rations with forage-to-concentrate ratio of 55:45. Dry matter intake and milk yield were recorded daily, and spot samples of urine, feces, and blood were collected at the end of each experimental period. Dry matter intake did not differ across diets, averaging 26.7 kg/d. Milk yield averaged 35.6 kg/d and was 1.1 kg/d greater for RCG versus FBG, and milk urea N concentration was lower for RCG compared with FBG. Milk yield was 2.2 kg/d and milk protein yield 66 g/d lower for FB versus RE. Nitrogen intake, urinary N, and urinary urea N excretions were lower, and milk N excretion tended to be lower for RCG compared with FBG. The proportion of the dietary N excreted as fecal N was larger in cows fed RCG than for those fed FBG, and the opposite was true for urinary N. We detected an interaction for milk N as percentage of N intake: it increased with RE compared with FB for RCG-based diet, but only a marginal increase was observed for FBG-based diet. Plasma concentration of His and Lys were lower for RCG than for FBG, whereas His tended to be greater and Lys lower for FB compared with RE. Further, plasma Met concentration was around 26% lower for FB than for RE. Of milk fatty acids, saturated fatty acids were decreased by RCG and increased by FB compared with FBG and RE, respectively, whereas monounsaturated fatty acids were increased by RCG versus FBG, and were lower for FB than for RE. In particular, 18:1n-9 concentration was lower for FB compared with RE. Polyunsaturated fatty acids, such as 18:2n-6 and 18:3n-3, were greater for RCG than for FBG, and 18:2n-6 was greater and 18:3n-3 was lower for FB versus RE. In addition, cis-9,trans-11 conjugated linoleic acid was lower for FB compared with RE. Faba bean whole-crop silage and faba bean meal have potential to be used as a part of dairy cow rations, but further research is needed to improve their N efficiency. Red clover-grass silage from a mixed sward, without inorganic N fertilizer input, combined with RE, resulted in the greatest N efficiency in the conditions of this experiment.

Biomass and Methane Production in Double Cereal Cropping Systems with Different Winter Cereal and Maize Plant Densities

The biogas supply chain requires a correct combination of crops to maximize the methane yield per hectare. Field trials were carried out in North Italy over three growing seasons, according to a factorial combination of four cropping systems (maize as a sole-crop or after hybrid barley, triticale and wheat) and two maize plant densities (standard, 7.5 plants m−2 and high, 10 plants m−2) with the plants harvested as whole-crop silage. The specific methane production per ton was measured through the biochemical methane potential (BMP) method, while the methane yield per hectare was calculated on the basis of the BMP results and considering the biomass yield. The average methane yield of wheat resulted to be equal to 4550 Nm3 ha−1, and +17% and +28% higher than triticale and barley, respectively, according to the biomass yield. A delay in maize sowing reduced the yield potential of this crop; the biomass of maize grown after barley, triticale and wheat was 20%, 33% and 47% lower, respectively, than maize cultivated as a single crop. The high plant population increased the biomass yield in the sole-crop maize (+23%) and in the maize grown after barley (+20%), compared to the standard density. The highest biomass (32 t ha−1 DM) and methane yield (9971 Nm3 ha−1) within the cropping systems were obtained for barley followed by maize at a high plant density. This cropping system increased the methane yield by 46% and 18%, respectively, compared to the sole-crop maize or maize after triticale at a standard density. The smaller amount of available solar radiation, resulting from the later sowing of maize, reduced the advantage related to the application of a high plant density.

Research and Control Techniques for Volunteer Rice

Recently, the Japanese government policy has recommended the cultivation of multipurpose high-yield paddy rice cultivars for animal feed (not only in the form of brown rice but also of whole crop silage (WCS) and others) in response to the decrease in demand on palatable rice for human consumption in Japan. However, when palatable cultivars are cropped in the following year of cultivations of high-yield cultivars for animal feed, the seeds of the high-yield cultivars are often left in the paddy field and grow as volunteer rice in the next crop season over winter, resulting in decrease in grain yield and lowering the quality of the palatable rice. Up to date, many ecological studies on overwintering ability of rice seeds have been carried out, and several key techniques on how to reduce volunteer rice were developed. This review summarizes ecological characteristics of volunteer rice, and describes effective countermeasures with cultural method, herbicides, lime nitrogen, and premature harvest (for only WCS).

Development of QAgriSupport, a GIS-based Agricultural Production Process Management System, and Foregis, a Mobile Application

In Japan, subsistence feed production has gained momentum in recent years. There has been an increase in the establishment of feed production contractors and efforts by cultivating farmers to grow feed crops, which are then harvested and purchased by contractors and others. These efforts serve a large production scale where the target fields are dispersed over a wide area. Thus, it is challenging to manage the work progress, which is a significant issue in the production field. Multipurpose process management methods and software are available for general manufacturing industries. However, applications for agricultural production, especially those that allow process management while displaying field information on a map, are expensive and lack sufficient functionality. To address these issues, this study developed a free, map-based agricultural production process management system, QAgriSupport, and a mobile application, Foregis, which can be coupled with QAgriSupport for agricultural enterprises that are large-scale and have widely dispersed fields. These applications have been introduced at a farm-type TMR (total mixed ration) center engaged in large-scale double-cropping of corn and a large-scale rice production corporation engaged in rice WCS (whole crop silage) and forage rice production. In addition, these applications have made a significant contribution to agricultural production process management by improving the efficiency of field-to-field movement and reducing the time required for work orders.

Physical, fermentative, and nutritional quality of silages made from three Sorghum bicolor varieties as affected by ensiling duration in South-west Nigeria.

Despite large-scale sorghum production in Nigeria, its utilization as livestock feed is limited to the stover following grain harvest. Therefore, we evaluated the physical, fermentative, and nutritive quality of whole-crop silages from three Sorghum bicolor varieties at different ensiling durations. The experiment was 3×5 factorial comprising three varieties (Samsorg14, Samsorg17, and Samsorg41) and five ensiling durations (0, 4, 8, 12, and 16 weeks). Forages were ensiled at the dough stage, and the silos were opened at the pre-determined durations for quality analyses. Samsorg14 silage recorded higher pH (5.88) and significant titratable acidity (8.32 g kg-1), while the least pH was observed for Samsorg17 silage (4.63). The forages ensiled for 8 weeks had a higher pH (5.04) compared with 4.51, 5.03, and 4.57 recorded at 4, 12, and 16 weeks, respectively. In contrast, forages ensiled for 4 weeks recorded the highest titratable acidity (8.39) and Flieg point (104.07). CP content was higher in fresh Samsorg17 (110.64 g kg-1) and lower (71.01 g kg-1) in Samsorg41 ensiled for 8 weeks as influenced by variety × ensiling duration. Cumulative gas volume and methane were higher for Samsorg41 silage (21.21 and 6.76 ml 200 mg-1 DM respectively). Ensiling for 16 weeks resulted in higher silages' IVDMD (44.00%) compared with other ensiling durations. Samsorg14 and Samsorg17 had a relatively stable silage pH, higher CP, and digestibility. Therefore, their silages could be conserved up to 16 weeks to provide high-quality feed for ruminants during the dry season to maintain animal productivity and ultimately enhance food security.

Feed Value of Whole-Crop Silage Rice by Cultivars and Plant Parts at Different Transplanting and Harvest Dates

Feed Value of Whole-Crop Silage Rice by Cultivars and Plant Parts at Different Transplanting and Harvest Dates

Double Cropping Productivity of Main Whole-Crop Silage Rice and Winter Feed Crops in the Central Plains of Korea

Double Cropping Productivity of Main Whole-Crop Silage Rice and Winter Feed Crops in the Central Plains of Korea

Effects of ethyl ester supplementation to forage on short-term dry matter intake and preference by goats

ABSTRACTIn whole-crop maize silages with atypical smell and decreased acceptance by ruminants, high concentrations of the volatile organic compounds ethyl acetate (EA) and ethyl lactate (EL) were detected. The aim of this study was to evaluate the impact of different concentrations of ethyl esters added to forage on preference and short-term feed intake of goats. In the first of three trials, whole-crop maize silage was supplemented with different concentrations of EA and EL and then vacuum-stored before use. Forages sampled during the preference trial showed a good recovery of EL with a high accordance of target (naturally formed + supplemented) and analysed concentrations. Supplemented EA was not recovered, making transient storage of substrates before use in feeding trials equivocal. However, four treatments with different concentrations of EL (approximately 330, 560, 920 and 1300 mg/kg dry matter (DM)) were used for the preference trial. In Trials 2 and 3, EA and EL (with and without ethanol, respectively) were added to grass hay directly before offering the feed, each in concentrations of 0, 600 and 1200 mg/kg DM to have six treatments each. In all trials, each possible combination of treatments was offered to Saanen-type wethers (n = 10, Trial 1; n = 5, Trials 2 and 3) as free choice in preference trials. In Trial 1, there was only a weak impact of EL on preference behaviour as goats avoided medium EL concentrations, but did not avoid silages with higher concentrations. In Trials 2 and 3, there was no influence of added volatiles on short-term DM intake and preference at all. It can be concluded that it is unlikely that ethyl esters as single substance or in combination with ethanol affect preference behaviour and feed intake of ruminants. Possibly a combination or still unidentified fermentation products cause avoidance instead of a single compound.

Comprehensive evaluation of growth performance and meat characteristics of a fattening system combining grazing with feeding rice whole-crop silage in Japanese Black steers.

We investigated the effect of a system for fattening steers combining grazing with feeding rice whole‐crop silage (rWCS) on growth performance, meat characteristics, and the expression of genes involved in skeletal muscle growth. Steers were randomly assigned to grazing or concentrate‐fed groups (CT). The grazing group (GZ) was fed rWCS after grazing until 16 months of age. The final body weight was the same in the two groups, but the dressed weight was lower in the GZ than in the CT. The beef color standard was higher in the GZ than in the CT. Although beef marbling did not differ between the two groups, there was less intramuscular fat and subcutaneous fat in the GZ than in the CT. The α‐tocopherol and β‐carotene contents in the muscle were higher in the GZ than in the CT. The GZ showed a lower daily gain (DG) during the grazing period, which may have resulted from decelerating skeletal muscle growth caused by the increased expression of genes encoding myostatin and atrogin‐1. However, the DG and feed efficiency of the GZ increased after grazing. The two groups exhibited a similar level of beef productivity.

The Relationship among Preference, Chemical-components and Fermentation-products of Apple-pomace Added Rice Whole-crop Silages

The Relationship among Preference, Chemical-components and Fermentation-products of Apple-pomace Added Rice Whole-crop Silages

Feed Value and Yield Potential of Main Whole-Crop Silage Rice Cultivars with Harvesting Time in the Central Plains of Korea

Feed Value and Yield Potential of Main Whole-Crop Silage Rice Cultivars with Harvesting Time in the Central Plains of Korea

Effects of offering grass silage alone or in combination with lupin/triticale, lupin/wheat or pea/oat whole-crop silages on animal performance, meat quality and fatty acid composition of beef from cattle offered two levels of concentrate

AbstractAn experiment was carried out to examine the effects of offering beef cattle five silage diets. These were perennial ryegrass silage (PRGS) as the sole forage, tall fescue/perennial ryegrass silage (FGS) as the sole forage, PRGS in a 50:50 ratio on a dry matter (DM) basis with lupin/triticale silage (LTS), lupin/wheat silage (LWS) and pea/oat silage (POS). Each of the five silage diets was supplemented with 4 and 7 kg of concentrates/head/day in a five silages × two concentrate intakes factorial design. A total of 90 cattle were used in the 121-day experiment. The grass silages were of medium digestibility and were well preserved. The legume/cereal silages had high ammonia N, high acetic acid, low lactic acid, low butyric acid and low digestible organic matter concentrations (542, 562 and 502 g/kg DM for LTS, LWS and POS, respectively). Silage treatment did not significantly affect liveweight gain, carcass gain, carcass characteristics, the instrumental assessment of meat quality or fatty acid composition of theM. longissimus dorsimuscle. In view of the low yields of the legume/cereal crops, it is concluded that the inclusion of spring-sown legume/cereal silages in the diets of beef cattle is unlikely to be advantageous.

飼料用イネ栽培ほ場における病害虫の発生実態 第1報 主要病害の発生実態

飼料用イネ栽培ほ場における病害虫の発生実態 第1報 主要病害の発生実態

飼料用イネ栽培ほ場における病害虫の発生実態 第2報 主要害虫の発生実態

飼料用イネ栽培ほ場における病害虫の発生実態 第2報 主要害虫の発生実態

窒素施肥と収穫時期が稲発酵粗飼料用品種「たちすずか」の材料草およびサイレージの化学成分と発酵品質に及ぼす影響

窒素施肥と収穫時期が稲発酵粗飼料用品種「たちすずか」の材料草およびサイレージの化学成分と発酵品質に及ぼす影響

Characterization of sugar metabolism in the stem of Tachisuzuka, a whole-crop silage rice cultivar with high sugar content in the stem

Tachisuzuka, a rice cultivar for whole-crop silage, is characterized by a small panicle and high sugar content in the stem. Our previous study suggests that the high sugar content in the stem of Tachisuzuka is due to a factor other than the small panicle. To characterize sugar metabolism in the stem of Tachisuzuka, here we compared carbohydrate content, enzyme activity, and the expression of genes involved in sugar metabolism in the stem between Tachisuzuka and its parental variety, Kusanohoshi. Thinning the panicles of Kusanohoshi increased the starch content in the leaf sheath and internode but did not increase the sucrose content in the leaf sheath to the same level as that of Tachisuzuka. This suggests that Tachisuzuka has high potential to accumulate sucrose in its leaf sheath. Comparison of enzyme activity showed that the hexokinase activities in the leaf sheath tended to be higher in Tachisuzuka than Kusanohoshi or panicle-thinned Kusanohoshi, suggesting that glucokinase or fructokinase affects sugar accumulation in the stem of Tachisuzuka. Comparative transcriptome analysis revealed the differences in expression levels of carbohydrate-related genes between Tachisuzuka and Kusanohoshi. In particular, the expression levels of ISA2, which encodes starch-debranching enzyme, and TMT2, which encodes tonoplast monosaccharide transporter – both of which maybe involved in sugar accumulation in grass stems – were higher in Tachisuzuka than Kusanohoshi. Thus, these enzymes and transporters may contribute to the high sugar content in the stem of Tachisuzuka.