White Oyster Mushroom Pleurotus Ostreatus Research Articles

Amplified Fragment Length Polymorphism Diversity of Cultivated White Oyster Mushroom Pleurotus ostreatus

Amplified fragment length Polymorphism (AFLP) analysis was performed to study the genetic diversity of fifteen isolates of white oyster mushroom ( Pleurotus ostreatus ) originated from different places in Indonesia (Java, Sumatra, Bali, and Kalimantan) and Thailand. The majority of studied isolates originated from commercial mushroom, and some of them were collected from nature. AFLP analysis revealed 202 loci of positive DNA bands. Heterozygosity value showed polymorphisms of the fifteen studied isolates. Correlation and cluster analyses of the isolates showed that isolate from Thailand (BNK isolate), isolate from Bogor (AMD isolate), and isolate from Purwokerto (USX isolate) were distinctly different from the other isolates indicated by positive bands with DNA size greater than 650 bp. Isolate from Kalimantan (BJM isolate) was distinctly different from Java island isolates. Generally there was no correlation of AFLP variability and geographic distribution of commercial strain isolates.

Evaluation of Alternate Substrate Pasteurization Techniques for Culinary-Medicinal White Oyster Mushroom, Pleurotus ostreatus var. florida (Agaricomycetideae) Cultivation

Three traditional substrate pasteurization methods (chemical, steam, and hot water) and seven alternate methods (solarization, botanicals, and sanitizers) were evaluated for the cultivation of culinary-medicinal white oyster mushroom Pleurotus ostreatus var. florida. The investigation was undertaken to scientifically evaluate current methods and to educate farmers to adopt appropriate methods that will not only prevent contamination and give optimal yields, but that will also be eco-friendly and pose no health hazards to the workers. Steam pasteurization (80°C, 2 h) was found to be the most efficient method and showed the highest biological efficiency (BE) at 82.8%, followed by the hot water method (80°C, 60 min) with BE at 77.6%. The chemical sterilization technique (500 ppm formaldehyde +75 ppm carbendazim-Bavistin) showed 49.6% BE and solarization 34.7% BE. All other treatments showed low BE. The hot-water, chemical sterilization, and solarization techniques were the most effective in preventing competitor molds.