White Noise Stimuli Research Articles

Enhanced auditory responses in visual cortex of blind rats using intrinsic optical signal imaging

Functional maturation of the visual cortex is induced by visual experiences during critical periods. Blind animals and humans exhibit improved auditory abilities after losing their vision. Here we investigated the response of the visual cortex to white noise stimuli during the progression of photoreceptor degeneration in a rat model of blindness (Royal College of Surgeons [RCS] (rdy/rdy) rats). Optical coherence tomography of RCS (+/+) rats with normal visual function revealed normal photoreceptor cells, whereas 3-month-old RCS (rdy/rdy) rats demonstrated photoreceptor cell degeneration. Visual cortex responses (VCRs) to a single flash stimulus were negligible in 3-month-old photoreceptor-degenerated rats. However, VCRs with white noise stimuli were significantly increased in blind versus RCS rats (+/+). Slight changes in the intrinsic optical signals of the control rats were observed on the ventral side of the visual cortex. In contrast, responses were markedly increased throughout the visual cortex of RCS (rdy/rdy) rats. These results indicate that the visual cortex rapidly acquires auditory system function over the first 3 months of life and that the entire visual cortex, rather than just the portion close to the auditory cortex, responds to white noise.

Contributions of associative and non-associative learning to the dynamics of defensive ethograms.

Defensive behavior changes based on threat intensity, proximity, and context of exposure, and learning about danger-predicting stimuli is critical for survival. However, most Pavlovian fear conditioning paradigms focus only on freezing behavior, obscuring the contributions of associative and non-associative mechanisms to dynamic defensive responses. To thoroughly investigate defensive ethograms, we subjected male and female adult C57BL/6J mice to a Pavlovian conditioning paradigm that paired footshock with a serial compound stimulus (SCS) consisting of distinct tone and white noise (WN) stimulus periods. To investigate how associative and non-associative mechanisms affect defensive responses, we compared this paired SCS-footshock group with four control groups that were conditioned with either pseudorandom unpaired presentations of SCS and footshock, shock only, or reversed SCS presentations with inverted tone--WN order, with paired or unpaired presentations. On day 2 of conditioning, the paired group exhibited robust freezing during the tone period with switching to explosive jumping and darting behaviors during the WN period. Comparatively, the unpaired and both reverse SCS groups expressed less tone-induced freezing and rarely showed jumping or darting during WN. Following the second day of conditioning, we observed how defensive behavior changed over two extinction sessions. During extinction, the tone-induced freezing decreased in the paired group and mice rapidly shifted from escape jumping during WN to a combination of freezing and darting. The unpaired, unpaired reverse, and shock-only groups displayed defensive tail rattling and darting during the SCS, with minimal freezing and jumping. Interestingly, the paired reverse group did not jump to WN, and tone-evoked freezing was resistant to extinction. These findings demonstrate that non-associative factors promote some defensive responsiveness, but associative factors are required for robust cue-induced freezing and high-intensity flight expression.

Defensive behaviors and c-fos expression in the midbrain.

Pavlovian fear conditioning serves as a valuable method for investigating species-specific defensive reactions (SSDRs) such as freezing and flight responses. The present study examines the role of white noise under different experimental conditions. Given that white noise has been shown to elicit both conditional (associative) and unconditional (nonassociative) defensive responses, we compared the response to noise following three separate training conditions: shock-only, white noise paired with shock, and context-only. Results showed that baseline freezing level significantly changed across groups: Both the shock-only group and the white noise paired with shock group froze more than the context-only group on the test day. White noise evoked differential freezing between groups on day 2: The shock-only group froze more than the context-only group although both groups were never exposed to white noise during training. Further, an activity burst triggered by white noise was similar for the shock-only and white noise paired with shock groups during testing, although shock-only group was never exposed to white noise stimuli during training. This aligned with c-fos data, indicating similar c-fos activity levels across different periaqueductal gray (PAG) regions for both shock-only and white noise paired with shock groups. However, the driving force behind c-fos activation-whether freezing, activity burst, or a combination of both-remains uncertain, warranting further analysis to explore specific correlations between SSDRs and c-fos activity within the PAG and related brain areas.

Weighted power summation and contrast normalization mechanisms account for short-latency eye movements to motion and disparity of sine-wave gratings and broadband visual stimuli in humans.

In this paper, we show that the model we proposed earlier to account for the disparity vergence eye movements (disparity vergence responses, or DVRs) in response to horizontal and vertical disparity steps of white noise visual stimuli also provides an excellent description of the short-latency ocular following responses (OFRs) to broadband stimuli in the visual motion domain. In addition, we reanalyzed the data and applied the model to several earlier studies that used sine-wave gratings (single or a combination of two or three gratings) and white noise stimuli. The model provides a very good account of all of these data. The model postulates that the short-latency eye movements-OFRs and DVRs-can be accounted for by the operation of two factors: an excitatory drive, determined by a weighted sum of contributions of stimulus Fourier components, scaled by a global contrast normalization mechanism. The output of the operation of these two factors is then nonlinearly scaled by the total contrast of the stimulus. Despite different roles of disparity (horizontal and vertical) and motion signals in visual scene analyses, the earliest processing stages of these different signals appear to be very similar.

Estimating and approaching the maximum information rate of noninvasive visual brain-computer interface

An essential priority of visual brain-computer interfaces (BCIs) is to enhance the information transfer rate (ITR) to achieve high-speed communication. Despite notable progress, noninvasive visual BCIs have encountered a plateau in ITRs, leaving it uncertain whether higher ITRs are achievable. In this study, we used information theory to study the characteristics and capacity of the visual-evoked channel, which leads us to investigate whether and how we can decode higher information rates in a visual BCI system. Using information theory, we estimate the upper and lower bounds of the information rate with the white noise (WN) stimulus. Consequently, we found out that the information rate is determined by the signal-to-noise ratio (SNR) in the frequency domain, which reflects the spectrum resources of the channel. Based on this discovery, we propose a broadband WN BCI by implementing stimuli on a broader frequency band than the steady-state visual evoked potentials (SSVEPs)-based BCI. Through validation, the broadband BCI outperforms the SSVEP BCI by an impressive 7 bps, setting a record of 50 bps. The integration of information theory and the decoding analysis presented in this study offers valuable insights applicable to general sensory-evoked BCIs, providing a potential direction of next-generation human-machine interaction systems.

Single opsin driven white noise ERGs in mice.

Electroretinograms elicited by photopigment isolating white noise stimuli (wnERGs) in mice were measured. The dependency of rod- and cone-opsin-driven wnERGs on mean luminance was studied. Temporal white noise stimuli (containing all frequencies up to 20 Hz, equal amplitudes, random phases) that modulated either rhodopsin, S-opsin or L*-opsin, using the double silent substitution technique, were used to record wnERGs in mice expressing a human L*-opsin instead of the native murine M-opsin. Responses were recorded at 4 mean luminances (MLs).Impulse response functions (IRFs) were obtained by cross-correlating the wnERG recordings with the corresponding modulation of the photopigment excitation elicited by the stimulus. So-called modulation transfer functions (MTFs) were obtained by performing a Fourier transform on the IRFs.Potentials of two repeated wnERG recordings at corresponding time points were plotted against each other. The correlation coefficient (r2repr) of the linear regression through these data was used to quantify reproducibility. Another correlation coefficient (r2ML) was used to quantify the correlations of the wnERGs obtained at different MLs with those at the highest (for cone isolating stimuli) or lowest (for rod isolating stimuli) ML. IRFs showed an initial negative (a-wave like) trough N1 and a subsequent positive (b-wave like) peak P1. No oscillatory potential-like components were observed. At 0.4 and 1.0 log cd/m2 ML robust L*- and S-opsin-driven IRFs were obtained that displayed similar latencies and dependencies on ML. L*-opsin-driven IRFs were 2.5-3 times larger than S-opsin-driven IRFs. Rhodopsin-driven IRFs were observed at -0.8 and - 0.2 log cd/m2 and decreased in amplitude with increasing ML. They displayed an additional pronounced late negativity (N2), which may be a correlate of retinal ganglion cell activity.R2repr and r2ML values increased for cones with increasing ML whereas they decreased for rods. For rhodopsin-driven MTFs at low MLs and L*-opsin-driven MTFs at high MLs amplitudes decreased with increasing frequency, with much faster decreasing amplitudes for rhodopsin. A delay was calculated from MTF phases showing larger delays for rhodopsin- vs. low delays for L*-opsin-driven responses. Opsin-isolating wnERGs in mice show characteristics of different retinal cell types and their connected pathways.

Onset-offset cortical auditory evoked potential amplitude differences indicate auditory cortical hyperactivity and reduced inhibition in people with tinnitus

ObjectiveThe current study investigates evidence of hypothesized reduced central inhibition and/or increased excitation in individuals with tinnitus by evaluating cortical auditory onset versus offset responses. MethodsCortical auditory evoked potentials (CAEPs) were recorded to the onset and offset of 3-second white noise stimuli in tinnitus and control groups matched in pairs by age, hearing, and sex (n = 26 total). Independent t-tests and 2-way mixed model ANOVA were used to evaluate onset-offset differences in amplitude, area, and latency of CAEP components by group. The predictive influence of tinnitus presence and associated participant characteristics on CAEP outcomes was assessed by multiple regression proportional reduction in error. ResultsThe tinnitus group had significantly larger onset minus offset P2 amplitudes (ΔP2 amplitudes) than control group participants. No other component variables differed significantly. ΔP2 amplitude was best predicted by tinnitus status and not significantly influenced by other variables such as hearing loss or age. ConclusionsHypothesized reduced central inhibition and/or increased excitation in tinnitus participants was partially supported by a group difference in ΔP2 amplitude. SignificanceThis was the first study to evaluate CAEP onset minus offset differences to investigate changes in central excitation/inhibition in individuals with tinnitus versus controls in matched groups.

EEG Interchannel Causality to Identify Source/Sink Phase Connectivity Patterns in Developmental Dyslexia.

While the brain connectivity network can inform the understanding and diagnosis of developmental dyslexia, its cause-effect relationships have not yet enough been examined. Employing electroencephalography signals and band-limited white noise stimulus at 4.8 Hz (prosodic-syllabic frequency), we measure the phase Granger causalities among channels to identify differences between dyslexic learners and controls, thereby proposing a method to calculate directional connectivity. As causal relationships run in both directions, we explore three scenarios, namely channels' activity as sources, as sinks, and in total. Our proposed method can be used for both classification and exploratory analysis. In all scenarios, we find confirmation of the established right-lateralized Theta sampling network anomaly, in line with the assumption of the temporal sampling framework of oscillatory differences in the Theta and Gamma bands. Further, we show that this anomaly primarily occurs in the causal relationships of channels acting as sinks, where it is significantly more pronounced than when only total activity is observed. In the sink scenario, our classifier obtains 0.84 and 0.88 accuracy and 0.87 and 0.93 AUC for the Theta and Gamma bands, respectively.

Modulation of narrow-field amacrine cells on light-evoked spike responses and receptive fields of retinal ganglion cells

By using multi-electrode array (MEA) recording technique in conjunction with white-noise checkerboard stimuli and reverse correlation methods, we studied modulatory actions of glycinergic narrow-field amacrine cells (NFACs) on spatiotemporal profiles of five functional groups of ganglion cells (GCs) in dark-adapted mouse retinas. We found that application of 2 µM strychnine significantly altered light-evoked spike rates of three groups of GCs. It also decreased receptive field center radii of all five groups of GC by a mean value of 11%, and shifted the GC receptive field (RF) centers of all GCs and the mean shift distances for the sustained GCs are significantly longer than the transient GCs. On the other hand, strychnine did not affect temporal profiles of the GC center responses, as it did not alter the time-to-peak or the biphasic index of the spike triggered average (STA) functions of GC RF centers. Strychnine also exerts limited actions on RF surrounds of most GCs, except that it moderately weakens the antagonistic surround of sustained OFF GCs and strengthens the antagonistic surround of the ON/OFF GCs, possibly through serial connections between NFACs and GABAergic wide-field amacrine cells (WFACs). Using the Sum of Separable Subfilter (SoSS) model and singular value decomposition method, we decomposed GCs’ STAs into five space–time separable subfilters, studied the observation rates of each subfilter in the five functional groups of GCs and determined NFAC-dependent and -independent synaptic circuitries that mediate center and surround responses of various groups of mouse retina retinal ganglion cells.

Stimulus-evoked and resting-state alpha oscillations show a linked dependence on patterned visual experience for development

Persistent visual impairments after congenital blindness due to dense bilateral cataracts have been attributed to altered visual cortex development within a sensitive period. Occipital alpha (8–14 Hz) oscillations were found to be reduced after congenital cataract reversal, while participants performed visual motion tasks. However, it has been unclear whether reduced alpha oscillations were task-specific, or linked to impaired visual behavior in cataract-reversed individuals. Here, we compared resting-state and stimulus-evoked alpha activity between individuals who had been treated for dense bilateral congenital cataracts (CC, n = 13, mean duration of blindness = 11.0 years) and age-matched, normally sighted individuals (SC, n = 13). We employed the visual impulse response function, adapted from VanRullen and MacDonald (2012), to test for the characteristic alpha response to visual white noise. Participants observed white noise stimuli changing in luminance with equal power at frequencies between 0 and 30 Hz. Compared to SC individuals, CC individuals demonstrated a reduced likelihood of exhibiting an evoked alpha response. Moreover, stimulus-evoked alpha power was reduced and correlated with a corresponding reduction of resting-state alpha power in the same CC individuals. Finally, CC individuals with an above-threshold evoked alpha peak had better visual acuity than CC individual without an evoked alpha peak. Since alpha oscillations have been linked to feedback communication, we suggest that the concurrent impairment in resting-state and stimulus-evoked alpha oscillations indicates an altered interaction of top-down and bottom-up processing in the visual hierarchy, which likely contributes to incomplete behavioral recovery in individuals who experienced transient congenital blindness.

Stimulator compensation and generation of Gaussian noise stimuli with defined amplitude spectra for studying input-output relations of sensory systems.

Gaussian noise is an important stimulus for the study of biological systems, especially sensory and neural systems. Since these systems are inherently nonlinear, the properties of the noise strongly influence the outcome of the analysis. Therefore, it is crucial to use a well-defined and controlled noise stimulus. In this paper, we first use the example of an insect filiform sensillum, a simple mechanoreceptor with a single sensory cell, to show that changes in the amplitude and spectral properties of the noise stimulus indeed affect the linear transfer function of the sensillum. We then explain step-by-step how to use the inverse fast Fourier transform to generate a Gaussian noise that has an arbitrary user-defined amplitude spectrum, including a band-limited white noise with a perfectly sharp cutoff edge. Finally, we demonstrate how such a perfect band-limited Gaussian white noise stimulus can also be generated with a non-perfect stimulator using a simple procedure that compensates for the filtering properties of the stimulator. With this approach, one can generate well-defined Gaussian noise stimuli that can be adapted to any application. For example, one can generate visual, sound, or vibrational stimuli for experimental research in visual physiology, auditory physiology, and biotremology, as well as inputs for testing various models in theoretical research.

Luminance white noise electroretinograms (wnERGs) in mice.

To record and analyse electroretinograms (ERGs) to luminance stimuli with white noise temporal profiles in mice. White noise stimuli are expected to keep the retina in a physiologically more natural state than, e.g., flashes. The influence of mean luminance (ML) was studied. Electroretinograms to luminance temporal white noise (TWN) modulation (wnERGs) were measured. The white noise stimuli contained all frequencies up to 20 Hz with equal amplitudes and random phases. Responses were recorded at 7 MLs between -0.7 and 1.2 log cd/m2. Impulse response functions (IRFs) were calculated by cross correlating the averaged white noise electroretinogram (wnERG) responses with the stimulus. Amplitudes and latencies of the initial trough and subsequent peak in the IRFs were measured at each ML. Fourier transforms of the IRFs resulted in modulation transfer functions (MTFs). wnERGs were averaged across different animals. They were measured twice and the responses at identical instances in the 1st and 2nd recordings were plotted against each other. The correlation coefficient (r 2 repr) of the linear regression quantified the reproducibility. The results of the first and second measurement were further averaged. To study the underlying ERG mechanisms, the ERG potentials at the different MLs were plotted against those at the lowest and highest ML. The correlation coefficients (r 2 ML) were used to quantify their similarities. The amplitudes of the initial (a-wave-like) trough of the IRFs increased with increasing ML. The following positive (b-wave-like) peak showed a minimum at -0.4 log cd/m2 above which there was a positive correlation between amplitude and ML. Their latencies decreased monotonously with increasing ML. In none of the IRFs, oscillatory potential (OP)-like components were observed. r 2 repr values were minimal at a ML of -0.1 log cd/m2, where the MTFs changed from low-pass to band-pass. r 2 ML values increased and decreased with increasing ML when correlated with responses obtained at the highest or the lowest ML, respectively. White noise electroretinograms can be reliably recorded in mice with luminance stimuli. IRFs resemble flash ERGs superficially, but they offer a novel procedure to study retinal physiology. New components can be described in the IRFs. The wnERGs are either rod- or cone-driven with little overlap.

Weighted summation and contrast normalization account for short-latency disparity vergence responses to white noise stimuli in humans.

Natural images are typically broadband, whereas detectors in early visual processing are selective for narrow ranges of spatial frequency. White noise patterns are widely used in laboratory settings to investigate how responses are derived from Fourier components in the image. Here, we report disparity vergence responses (DVRs) to white noise stimuli in human subjects and compare these with responses to white noise patterns filtered with bandpass filters and notch filters and to sinusoidal gratings. Although the contribution of these short-latency eye movements to the overall vergence response to a given stimulus is generally small, they have proven to be a valuable tool for the study of the early mechanisms that process disparity stimuli in human subjects. Removing lower spatial frequency (SF) components reduced DVR amplitude, whereas removing higher SF components led to an increase in DVR amplitude. For larger disparities, the transition occurred at lower SFs. All of these effects were quantitatively well described by a model that combined two factors: (a) an excitatory drive determined by a weighted sum of stimulus Fourier components, which was scaled by (b) a contrast normalization mechanism.

The Effects of White Noise on Attentional Performance and On-Task Behaviors in Preschoolers with ADHD.

Several models have tentatively associated improving attention-deficit/hyperactivity disorder (ADHD) symptoms with arousal and external environmental stimulation. In order to further clarify the relationships between ADHD symptoms, arousal, and external stimulation, this study focused on exploring the "simultaneous" effects of white noise on intrinsic attentional performance and extrinsic on-task behaviors in preschoolers with and without ADHD. By using the computerized task (K-CPT 2), 104 preschoolers, including 52 ADHD children and 52 typically developing (TD) children, were tested and analyzed for their intrinsic attention (such as detectability, omission errors, commission errors, and reaction time). Simultaneously, these preschoolers' external on-task behaviors were recorded for analysis through systematic observation. This study showed that white noise could effectively improve attention performance, including enhancing the ability to differentiate non-targets from targets and decreasing omission errors. It could also reduce the extrinsic hyperactive behaviors of preschoolers with ADHD. The findings of this study highlighted that white noise stimulation is a beneficial non-pharmacological treatment for preschoolers with ADHD. In contrast, for TD preschoolers, the results of this study showed that the external white noise stimuli were not only unhelpful but also a burden.

Something in the Sway: Effects of the Shepard-Risset Glissando on Postural Activity and Vection.

This study investigated claims of disrupted equilibrium when listening to the Shepard-Risset glissando (which creates an auditory illusion of perpetually ascending/descending pitch). During each trial, 23 participants stood quietly on a force plate for 90s with their eyes either open or closed (30s pre-sound, 30s of sound and 30s post-sound). Their centre of foot pressure (CoP) was continuously recorded during the trial and a verbal measure of illusory self-motion (i.e., vection) was obtained directly afterwards. As expected, vection was stronger during Shepard-Risset glissandi than during white noise or phase-scrambled auditory control stimuli. Individual differences in auditorily evoked postural sway (observed during sound) were also found to predict the strength of this vection. Importantly, the patterns of sway induced by Shepard-Risset glissandi differed significantly from those during our auditory control stimuli - but only in terms of their temporal dynamics. Since significant sound type differences were not seen in terms of sway magnitude, this stresses the importance of investigating the temporal dynamics of sound-posture interactions.

Electroretinographic responses to luminance and cone-isolating white noise stimuli in macaques.

Electroretinograms (ERGs) are mass potentials with a retinal origin that can be measured non-invasively. They can provide information about the physiology of the retina. Often, ERGs are measured to flashes that are highly unnatural stimuli. To obtain more information about the physiology of the retina, we measured ERGs with temporal white noise (TWN) stimuli that are more natural and keep the retina in a normal range of operation. The stimuli can be combined with the silent substitution stimulation technique with which the responses of single photoreceptor types can be isolated. We characterized electroretinogram (ERG) responses driven by luminance activity or by the L- or the M-cones. The ERGs were measured from five anesthetized macaques (two females) to luminance, to L-cone isolating and to M-cone isolating stimuli in which luminance or cone excitation were modulated with a TWN profile. The responses from different recordings were correlated with each other to study reproducibility and inter-individual variability. Impulse response functions (IRFs) were derived by cross-correlating the response with the stimulus. Modulation transfer functions (MTFs) were the IRFs in the frequency domain. The responses to luminance and L-cone isolating stimuli showed the largest reproducibility. The M-cone driven responses showed the smallest inter-individual variability. The IRFs and MTFs showed early (high frequency) components that were dominated by L-cone driven signals. A late component was equally driven by L- and M-cone activity. The IRFs showed characteristic similarities and differences relative to flash ERGs. The responses to TWN stimuli can be used to characterize the involvement of retinal cells and pathways to the ERG response. It can also be used to identify linear and non-linear processes.

A mechanistically interpretable model of the retinal neural code for natural scenes with multiscale adaptive dynamics.

The visual system processes stimuli over a wide range of spatiotemporal scales, with individual neurons receiving input from tens of thousands of neurons whose dynamics range from milliseconds to tens of seconds. This poses a challenge to create models that both accurately capture visual computations and are mechanistically interpretable. Here we present a model of salamander retinal ganglion cell spiking responses recorded with a multielectrode array that captures natural scene responses and slow adaptive dynamics. The model consists of a three-layer convolutional neural network (CNN) modified to include local recurrent synaptic dynamics taken from a linear-nonlinear-kinetic (LNK) model [1]. We presented alternating natural scenes and uniform field white noise stimuli designed to engage slow contrast adaptation. To overcome difficulties fitting slow and fast dynamics together, we first optimized all fast spatiotemporal parameters, then separately optimized recurrent slow synaptic parameters. The resulting full model reproduces a wide range of retinal computations and is mechanistically interpretable, having internal units that correspond to retinal interneurons with biophysically modeled synapses. This model allows us to study the contribution of model units to any retinal computation, and examine how long-term adaptation changes the retinal neural code for natural scenes through selective adaptation of retinal pathways.

Neural mechanism of spatio-chromatic opponency in the Drosophila amacrine neurons

Visual animals detect spatial variations of light intensity and wavelength composition. Opponent coding is a common strategy for reducing information redundancy. Neurons equipped with both spatial and spectral opponency have been identified in vertebrates but not yet in insects. The Drosophila amacrine neuron Dm8 was recently reported to show color opponency. Here, we demonstrate Dm8 exhibits spatio-chromatic opponency. Antagonistic convergence of the direct input from the UV-sensing R7s and indirect input from the broadband receptors R1-R6 through Tm3 and Mi1 is sufficient to confer Dm8's UV/Vis (ultraviolet/visible light) opponency. Using high resolution monochromatic stimuli, we show the pale and yellow subtypes of Dm8s, inheriting retinal mosaic characteristics, have distinct spectral tuning properties. Using 2D white-noise stimulus and reverse correlation analysis, we found that the UV receptive field (RF) of Dm8 has a center-inhibition/surround-excitation structure. In the absence of UV-sensing R7 inputs, the polarity of the RF is inverted owing to the excitatory input from the broadband photoreceptors R1-R6. Using a new synGRASP method based on endogenous neurotransmitter receptors, we show that neighboring Dm8s form mutual inhibitory connections mediated by the glutamate-gated chloride channel GluClα, which is essential for both Dm8's spatial opponency and animals' phototactic behavior. Our study shows spatio-chromatic opponency could arise in the early visual stage, suggesting a common information processing strategy in both invertebrates and vertebrates.

Spatial and temporal resolution of optogenetically recovered vision in ChR2-transduced mouse retina

Objective. Retinal ganglion cells (RGCs) represent an attractive target in vision restoration strategies, because they undergo little degeneration compared to other retinal neurons. Here we investigated the temporal and spatial resolution in adult photoreceptor-degenerated (rd10) mouse retinas, where RGCs have been transduced with the optogenetic actuator channelrhodopsin-2 (ChR2). Approach. The RGC spiking activity was recorded continuously with a CMOS-based microelectrode array during a variety of photostimulation protocols. The temporal resolution was assessed through Gaussian white noise stimuli and evaluated using a linear-nonlinear-Poisson model. Spatial sensitivity was assessed upon photostimulation with single rectangular pulses stepped across the retina and upon stimulation with alternating gratings of different spatial frequencies. Spatial sensitivity was estimated using logistic regression or by evaluating the spiking activity of independent RGCs. Main results. The temporal resolution after photostimulation displayed an about ten times faster kinetics as compared to physiological filters in wild-type RGCs. The optimal spatial resolution estimated using the logistic regression model was 10 µm and 87 µm based on the population response. These values correspond to an equivalent acuity of 1.7 or 0.2 cycles per degree, which is better than expected from the size of RGCs’ optogenetic receptive fields. Significance. The high temporal and spatial resolution obtained by photostimulation of optogenetically transduced RGCs indicate that high acuity vision restoration may be obtained by photostimulation of appropriately modified RGCs in photoreceptor-degenerated retinas.

Absence of Nonlinear Coupling Between Electric Vestibular Stimulation and Evoked Forces During Standing Balance.

The vestibular system encodes motion and orientation of the head in space and is essential for negotiating in and interacting with the world. Recently, random waveform electric vestibular stimulation has become an increasingly common means of probing the vestibular system. However, many of the methods used to analyze the behavioral response to this type of stimulation assume a linear relationship between frequencies in the stimulus and its associated response. Here we examine this stimulus-response frequency linearity to determine the validity of this assumption. Forty-five university-aged subjects stood on a force-plate for 4 min while receiving vestibular stimulation. To determine the linearity of the stimulus-response relationship we calculated the cross-frequency power coupling between a 0 and 25 Hz bandwidth limited white noise stimulus and induced postural responses, as measured using the horizontal forces acting at the feet. Ultimately, we found that, on average, the postural response to a random stimulus is linear across stimulation frequencies. This result supports the use of analysis methods that depend on the assumption of stimulus-response frequency linearity, such as coherence and gain, which are commonly used to analyze the body’s response to random waveform electric stimuli.